AngiogenesisPub Date : 2025-03-18DOI: 10.1007/s10456-025-09974-8
Angelo Nigro
{"title":"Microvascular and cerebrovascular alterations in Raynaud’s phenomenon and fibromyalgia","authors":"Angelo Nigro","doi":"10.1007/s10456-025-09974-8","DOIUrl":"10.1007/s10456-025-09974-8","url":null,"abstract":"","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AngiogenesisPub Date : 2025-03-13DOI: 10.1007/s10456-025-09971-x
James T. Brash, Guillermo Diez-Pinel, Luca Rinaldi, Raphael F. P. Castellan, Alessandro Fantin, Christiana Ruhrberg
{"title":"Endothelial transcriptomic, epigenomic and proteomic data challenge the proposed role for TSAd in vascular permeability","authors":"James T. Brash, Guillermo Diez-Pinel, Luca Rinaldi, Raphael F. P. Castellan, Alessandro Fantin, Christiana Ruhrberg","doi":"10.1007/s10456-025-09971-x","DOIUrl":"10.1007/s10456-025-09971-x","url":null,"abstract":"<div><p>The vascular endothelial growth factor VEGF drives excessive vascular permeability to cause tissue-damaging oedema in neovascular and inflammatory diseases across multiple organs. Several molecular pathways have been implicated in VEGF-induced hyperpermeability, including binding of the VEGF-activated tyrosine kinase receptor VEGFR2 by the T-cell specific adaptor (TSAd) to recruit a SRC family kinase to induce junction opening between vascular endothelial cells (ECs). Inconsistent with a universal role for TSAd in permeability signalling, immunostaining approaches previously reported TSAd only in dermal and kidney vasculature. To address this discrepancy, we have mined publicly available omics data for expression of TSAd and other permeability-relevant signal transducers in multiple organs affected by VEGF-induced vascular permeability. Unexpectedly, TSAd transcripts were largely absent from EC single cell RNAseq data, whereas transcripts for other permeability-relevant signal transducers were detected readily. TSAd transcripts were also lacking from half of the EC bulk RNAseq datasets examined, and in the remaining datasets appeared at low levels concordant with models of leaky transcription. Epigenomic EC data located the TSAd promoter to closed chromatin in ECs, and mass spectrometry-derived EC proteomes typically lacked TSAd. By suggesting that TSAd is not actively expressed in ECs, our findings imply that TSAd is likely not critical for linking VEGFR2 to downstream signal transducers for EC junction opening.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09971-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AngiogenesisPub Date : 2025-03-06DOI: 10.1007/s10456-025-09973-9
David M. Smadja, Yanis Berkane, Nun K. Bentounes, Jeanne Rancic, Audrey Cras, Cécile Pinault, Marie Ouarne, Elise Paucod, Walid Rachidi, Alexandre G. Lellouch, Maxime Jeljeli
{"title":"Immune-privileged cord blood-derived endothelial colony-forming cells: advancing immunomodulation and vascular regeneration","authors":"David M. Smadja, Yanis Berkane, Nun K. Bentounes, Jeanne Rancic, Audrey Cras, Cécile Pinault, Marie Ouarne, Elise Paucod, Walid Rachidi, Alexandre G. Lellouch, Maxime Jeljeli","doi":"10.1007/s10456-025-09973-9","DOIUrl":"10.1007/s10456-025-09973-9","url":null,"abstract":"<div><p>Cord blood-derived endothelial colony-forming cells (CB-ECFCs) hold significant promise for regenerative medicine due to their unique vasculogenic and immunomodulatory properties. These cells exhibit a superior proliferative capacity, robust ability to form vascular networks, and lower immunogenicity compared to adult and embryonic stem cell-derived counterparts. The immune-privileged characteristics of CB-ECFCs, including reduced expression of pro-inflammatory mediators and tolerance-inducing molecules such as HLA-G, further enhance their therapeutic potential. Their low immunogenicity minimizes the risk of immune rejection, making them suitable for allogenic cell therapies. Their application extends to complex tissue engineering and organ revascularization, where their ability to integrate into three-dimensional scaffolds and support vascular tree formation represents a significant advancement. Moreover, CB-ECFCs’ capability to adapt to inflammatory stimuli and retain immunological memory highlights their functional versatility in dynamic microenvironments. This review highlights the remarkable ontogeny of ECFCs while unveiling the unparalleled potential of CB-ECFCs in revolutionizing regenerative medicine. From pre-vascularizing engineered tissues and organoids to pioneering cell-based therapies for cardiovascular, dermatological, and degenerative diseases, CB-ECFCs stand at the forefront of cutting-edge biomedical advancements, offering unprecedented opportunities for therapeutic innovation. By leveraging their vasculogenic, immune-regulatory, and regenerative capacities, CB-ECFCs offer a robust alternative for addressing the challenges of vascular repair and organ engineering. Future research should focus on unraveling their transcriptomic and functional profiles to optimize clinical applications and advance the field of regenerative medicine.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09973-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AngiogenesisPub Date : 2025-03-06DOI: 10.1007/s10456-025-09972-w
Sarah M. Moss, Thomas Gerton, Hannah A. Strobel, James B. Hoying
{"title":"Isolated human adipose microvessels retain native microvessel structure and recapitulate sprouting angiogenesis","authors":"Sarah M. Moss, Thomas Gerton, Hannah A. Strobel, James B. Hoying","doi":"10.1007/s10456-025-09972-w","DOIUrl":"10.1007/s10456-025-09972-w","url":null,"abstract":"<div><p>With interest growing in modeling more complex aspects of human disease in the laboratory, the need for effectively vascularizing human tissue models is becoming paramount. However, fully recreating human tissue microvasculatures is challenging given the multicellular complexity of the microvessel and microvessel-tissue interplay. Importantly, effective models should capture the dynamic activity of the perivascular cells of the perivascular niche, which are critical to tissue hemostasis and function. Isolated microvessel fragments from rodent adipose have been extensively studied and used in a variety of vascularization models. We have progressed this proven technology by deriving isolated fragments of intact human microvessels harvested from adipose (haMVs) to model human vascularization and advance human vascularized tissue models. Here we show the haMVs retain native microvessel structures, including perivascular cellularity, and recapitulate bona fide sprouting angiogenesis in vitro through distinct sprouting and neovessel elongation phases. As primary isolates, the angiogenic potential varies between donor lots and correlates with the presence of haMV perivascular cells. In an in vitro model of tumor angiogenesis, the addition of anti-tumor agents impacted tumor cell expansion in the presence of the haMVs but not endothelial cells alone demonstrating the importance of the perivascular cells in tissue modeling. The human adipose microvessels offer, in a single reagent, a more complex, dynamic human tissue model vascularization solution.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cxcl9 modulates aging associated microvascular metabolic and angiogenic dysfunctions in subcutaneous adipose tissue","authors":"Xin Fu, Yu Zhao, Xiwei Cui, Siyuan Huang, Yanze Lv, Chen Li, Fuxing Gong, Zhigang Yang, Xiaonan Yang, Ran. Xiao","doi":"10.1007/s10456-025-09970-y","DOIUrl":"10.1007/s10456-025-09970-y","url":null,"abstract":"<div><p>Microvascular aging, predominantly driven by endothelial cells (ECs) dysfunction, is a critical early event in cardiovascular diseases. However, the specific effects of aging on ECs across the microvascular network segments and the associated mechanisms are not fully understood. In this study, we detected a microvascular rarefaction and a decreased proportion of venular ECs in the subcutaneous adipose tissue of aged mice using light-sheet immunofluorescence microscopy and single-cell RNA sequencing. Moreover, aged ECs, especially in the venular subtype, exhibited a pseudotemporal transition to a terminal state characterized by diminished oxidative phosphorylation and strengthened cytokine signaling. Metabolic flux balance analysis predicted that among the 13 differentially expressed cytokines identified in aged EC subpopulations, Cxcl9 was strongly correlated with impaired oxidative phosphorylation in aged ECs. It was further validated using microvascular ECs treated with Cxcl9. Notably, the G protein-coupled receptor signaling pathway was subsequently suppressed, in which Aplnr suppression was also observed in aged ECs, contributing to their impaired energy metabolism and reduced angiogenesis. Based on these findings, we propose Cxcl9 as a biomarker for aging-related dysfunction of microvascular ECs, suggesting that targeting Cxcl9 signaling may help combat microvascular aging.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09970-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of liver sinusoidal endothelial cells in metabolic dysfunction-associated steatotic liver diseases and liver cancer: mechanisms and potential therapies","authors":"Hanjun Mo, Pengfei Yue, Qiaoqi Li, Yinxi Tan, Xinran Yan, Xinyue Liu, Yuanwei Xu, Yingzhe Luo, Suruiya Palihati, Cheng Yi, Hua Zhang, Minlan Yuan, Biao Yang","doi":"10.1007/s10456-025-09969-5","DOIUrl":"10.1007/s10456-025-09969-5","url":null,"abstract":"<div><p>Liver sinusoidal endothelial cells (LSECs), with their unique morphology and function, have garnered increasing attention in chronic liver disease research. This review summarizes the critical roles of LSECs under physiological conditions and in two representative chronic liver diseases: metabolic dysfunction-associated steatotic liver disease (MASLD) and liver cancer. Under physiological conditions, LSECs act as selective barriers, regulating substance exchange and hepatic blood flow. Interestingly, LSECs exhibit contrasting roles at different stages of disease progression: in the early stages, they actively resist disease advancement and help restore sinusoidal homeostasis; whereas in later stages, they contribute to disease worsening. During this transition, LSECs undergo capillarization, lose their characteristic markers, and become dysfunctional. As the disease progresses, LSECs closely interact with hepatocytes, hepatic stellate cells, various immune cells, and tumor cells, driving processes such as steatosis, inflammation, fibrosis, angiogenesis, and carcinogenesis. Consequently, targeting LSECs represents a promising therapeutic strategy for chronic liver diseases. Relevant therapeutic targets and potential drugs are summarized in this review.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AngiogenesisPub Date : 2025-02-03DOI: 10.1007/s10456-024-09965-1
Yuan Gu, Tianci Tang, Moqin Qiu, Hongmei Wang, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke
{"title":"Clioquinol inhibits angiogenesis by promoting VEGFR2 degradation and synergizes with AKT inhibition to suppress triple-negative breast cancer vascularization","authors":"Yuan Gu, Tianci Tang, Moqin Qiu, Hongmei Wang, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke","doi":"10.1007/s10456-024-09965-1","DOIUrl":"10.1007/s10456-024-09965-1","url":null,"abstract":"<div><p>Inhibition of angiogenesis, either as monotherapy or in conjunction with other treatments, holds significant promise in cancer treatment. However, the limited efficacy of clinically approved anti-angiogenic agents underscores the urgent need for the development of novel drugs and therapeutic strategies. In this study, we demonstrate the highly selective inhibitory effects of clioquinol, a topical antifungal and antibiotic agent, on the angiogenic activity of endothelial cells (ECs) in a series of in vitro angiogenesis assays. Moreover, clioquinol effectively suppressed blood vessel formation in ex vivo aortic ring and in vivo Matrigel plug assays. Mechanistic studies revealed that clioquinol directly binds to the ATP-binding site of vascular endothelial growth factor receptor 2 (VEGFR2), promoting its degradation through both proteasome and lysosome pathways. This led to the down-regulation of the downstream extracellular signal-regulated kinase (ERK) pathway. In addition, the combination with the AKT inhibitor MK-2206 synergistically boosted the anti-angiogenic efficacy of clioquinol in vitro and in an in vivo dorsal skinfold chamber model of triple-negative breast cancer (TNBC), leading to the suppression of TNBC growth. Accordingly, clioquinol, either alone or in combination with AKT inhibitors, represents a promising therapeutic agent for future anti-angiogenic cancer treatment.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-024-09965-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AngiogenesisPub Date : 2025-02-03DOI: 10.1007/s10456-024-09953-5
Ashely R. Ricciardelli, Gael Genet, Nafiisha Genet, Samuel T. McClugage III, Peter T. Kan, Karen K. Hirschi, Jason E. Fish, Joshua D. Wythe
{"title":"From bench to bedside: murine models of inherited and sporadic brain arteriovenous malformations","authors":"Ashely R. Ricciardelli, Gael Genet, Nafiisha Genet, Samuel T. McClugage III, Peter T. Kan, Karen K. Hirschi, Jason E. Fish, Joshua D. Wythe","doi":"10.1007/s10456-024-09953-5","DOIUrl":"10.1007/s10456-024-09953-5","url":null,"abstract":"<div><p>Brain arteriovenous malformations are abnormal vascular structures in which an artery shunts high pressure blood directly to a vein without an intervening capillary bed. These lesions become highly remodeled over time and are prone to rupture. Historically, brain arteriovenous malformations have been challenging to treat, using primarily surgical approaches. Over the past few decades, the genetic causes of these malformations have been uncovered. These can be divided into (1) familial forms, such as loss of function mutations in TGF-β (BMP9/10) components in hereditary hemorrhagic telangiectasia, or (2) sporadic forms, resulting from somatic gain of function mutations in genes involved in the RAS-MAPK signaling pathway. Leveraging these genetic discoveries, preclinical mouse models have been developed to uncover the mechanisms underlying abnormal vessel formation, and thus revealing potential therapeutic targets. Impressively, initial preclinical studies suggest that pharmacological treatments disrupting these aberrant pathways may ameliorate the abnormal pathologic vessel remodeling and inflammatory and hemorrhagic nature of these high-flow vascular anomalies. Intriguingly, these studies also suggest uncontrolled angiogenic signaling may be a major driver in bAVM pathogenesis. This comprehensive review describes the genetics underlying both inherited and sporadic bAVM and details the state of the field regarding murine models of bAVM, highlighting emerging therapeutic targets that may transform our approach to treating these devastating lesions.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-024-09953-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}