Angiogenesis最新文献

{"title":"Polysialic acid regulates glomerular microvasculature formation by interaction with VEGF-A188 in mice","authors":"Kristina M. Niculovic,&nbsp;Manuel M. Vicente,&nbsp;Vanessa Wittek,&nbsp;Elina Kats,&nbsp;Iris Albers,&nbsp;Kerstin Flächsig-Schulz,&nbsp;Ulrike Peters-Bernard,&nbsp;Anna-Carina Weiss,&nbsp;Hauke Thiesler,&nbsp;Laura S. Dräger,&nbsp;Manuel H. Taft,&nbsp;Anne Jörns,&nbsp;Hans Bakker,&nbsp;Herbert Hildebrandt,&nbsp;Martina Mühlenhoff,&nbsp;Birgit Weinhold,&nbsp;Markus Abeln,&nbsp;Anja K. Münster-Kühnel","doi":"10.1007/s10456-025-09984-6","DOIUrl":"10.1007/s10456-025-09984-6","url":null,"abstract":"<div><p>Vascular endothelial growth factor A (VEGF-A) is a key signalling protein that stimulates blood vessel development and repair. Its tight control is essential for organ development and tissue homeostasis. However, the complex regulatory network for balanced bioavailability of VEGF-A is not fully understood. Here, we assessed the role of the glycocalyx component polysialic acid (polySia) for kidney development and its potential interactions with VEGF-A isoforms, in vitro and in vivo, using mouse models of polySia deficiency. PolySia acts as negative regulator of cell adhesion, but also may interact with extracellular components. In murine kidney, polySia was identified on nephron progenitor and endothelial cell subsets in developing nephrons with declining expression during maturation. Loss of polySia in <i>Ncam</i>^{<i>−/−</i>} mice revealed the neural cell adhesion molecule NCAM as major protein carrier. Both polysialyltransferase-negative and <i>Ncam</i>^{<i>−/−</i>} mice displayed impaired glomerular microvasculature development with reduced endothelial cell numbers, reminiscent to the phenotype of mice with impaired VEGF-A signalling. In vitro, immobilized polySia specifically interacted with the VEGF-A188 isoform demonstrating an isoform-specific direct interaction. Single cell RNA sequencing data analysis of newborn mouse kidneys implicated activation of VEGF-A-signalling in polysialyltransferase-positive endothelial cells. Consistently, loss of polySia resulted in diminished VEGFR2 activation in perinatal kidney and human endothelial cells. At transcriptional level, the expression of polysialyltransferases and known polySia carrier proteins is conserved in human developing kidney. Together, these data demonstrate a direct impact of polySia on VEGF-A signalling with the perspective that polysialylation could be a therapeutic target to ameliorate microvasculature repair after renal injury.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09984-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human endothelial colony forming cells (ECFCs) require endothelial protein C receptor (EPCR) for cell cycle progression and angiogenic activity","authors":"Sarah E. J. Chambers,&nbsp;Jasenka Guduric-Fuchs,&nbsp;Edoardo Pedrini,&nbsp;Pietro M. Bertelli,&nbsp;Chutima Charoensuk,&nbsp;Elisa Peixoto,&nbsp;Varun Pathak,&nbsp;Hamza I. Alhamdan,&nbsp;Ruoxiao Xie,&nbsp;Anna Krasnodembskaya,&nbsp;Judith Lechner,&nbsp;Alan W. Stitt,&nbsp;Reinhold J. Medina","doi":"10.1007/s10456-025-09982-8","DOIUrl":"10.1007/s10456-025-09982-8","url":null,"abstract":"<div><p>Vascular repair and regeneration are critical for tissue homeostasis. Endothelial colony forming cells (ECFCs) are vessel-resident progenitors with vasoreparative capacity and they offer an important avenue for allogeneic cytotherapy to achieve perfusion of ischemic tissues. Endothelial Protein C Receptor (EPCR) has been proposed as a marker for vascular endothelial stem cells, but its precise role in ECFC biology remains unknown. The current study has investigated the biological relevance of EPCR in ECFC function. Our data show that over 95% of ECFCs exhibit high EPCR expression. These levels surpassing CD34 and CD157, positions EPCR as a new robust ECFC immunophenotypic marker, alongside established markers CD31 and CD105. Functionally, depleting EPCR expression in ECFCs significantly diminished angiogenic activity, including proliferation, migration and tube formation. This knockdown also altered normal ECFC barrier function. Transcriptomic analysis indicated that knockdown of EPCR led to enrichment of gene signatures for cell cycle, TGF beta, and focal adhesion kinases. G1 cell cycle arrest was confirmed in ECFCs with depleted EPCR. Mechanistically, EPCR knockdown led to increased release of TGFβ2 and SMAD2/3 activation, coupled with increased p21, decreased pFAK, and increased transgelin. Additionally, we showed that quiescent ECFCs showed significantly lower EPCR expression when compared to proliferating ECFCs. In agreement with this, cell sorting experiments demonstrated that ECFCs with the highest EPCR expression exhibited the highest clonogenic capacity. In summary, our findings highlight that EPCR expression in ECFCs is critical for their angiogenic activity, by modulating cell cycle progression.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09982-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute aortic dissection in a patient with Hereditary Hemorrhagic Telangiectasia associated with Juvenile Polyposis due to SMAD4 mutation: case report and literature review","authors":"Catalina Pezzoto,&nbsp;Ana Braslavsky,&nbsp;Carolina Vázquez,&nbsp;Candelaria Serrano,&nbsp;Marcelo Serra","doi":"10.1007/s10456-025-09981-9","DOIUrl":"10.1007/s10456-025-09981-9","url":null,"abstract":"<div><h3>Objective</h3><p>We present a novel case of concurrent Hereditary Hemorrhagic Telangiectasia-Juvenile Polyposis Syndrome (HHT-JP), resulting in a fatal aortic dissection. Given rarity of the case, we aimed to perform a comprehensive review of the existing literature to better characterize this clinical complication in this population.</p><h3>Methods</h3><p>We conducted a literature review on HHT-JP syndrome using PubMed, focusing on English-language articles published between 2010 and 2024, specifically case reports and small series. Search terms \"Hereditary Hemorrhagic Telangiectasia\", “Osler-Weber-Rendu syndrome” and \"Juvenile Polyposis\" were used. Exclusion criteria included population studies lacking detailed individual characteristics related to Hereditary Hemorrhagic Telangiectasia (HHT) or Juvenile Polyposis (JP). Duplicate articles were removed, and data were extracted on patient demographics, clinical presentations, diagnostic criteria (Curaçao criteria for HHT, Jass criteria for JP), treatments, and outcomes.</p><h3>Results</h3><p>Fifty-six individuals with the MADH4 mutation met the inclusion criteria and were compared to our patient. The age range of the total cohort of fifty-six participants spanned from 6 to 66 years, with a distribution between men and women. The typical clinical presentation of HHT-JP was observed in most cases. Notably, only one patient from previous literature exhibited aortic dissection, aligning with our patient's presentation. Additionally, three other patients had aortic aneurysms. Musculoskeletal and other cardiovascular anomalies were also identified and described.</p><h3>Discussion</h3><p>While aortic aneurysms prevail in HHT-JP syndrome, aortic dissection cases are extremely rare. This case highlights the need for vigilant screening to identify aortic anomalies in this specific patient subset, emphasizing the severe complications associated with this syndrome combination.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SDF-1α mRNA therapy in peripheral artery disease","authors":"Tinghong Zhang,&nbsp;Binqiang Zhu,&nbsp;Shijie Deng,&nbsp;Jinling Qin,&nbsp;Jingyuan Zhang,&nbsp;Shu Meng","doi":"10.1007/s10456-025-09979-3","DOIUrl":"10.1007/s10456-025-09979-3","url":null,"abstract":"<div><p>Stromal cell-derived factor-1 alpha (SDF-1α) is a promising target for therapeutic angiogenesis in ischemic diseases such as peripheral artery disease (PAD). However, the clinical application of SDF-1α protein or plasmid-based gene therapy is unsuccessful. mRNA therapy has shown great promise in protein replacement. Here, we developed DOPE-lipid nanoparticles (LNPs) encapsulating SDF-1α mRNA (LNP@SDF-1α) for efficient gene delivery. In vitro, LNP@SDF-1α treatment of human umbilical vein endothelial cells (HUVECs) significantly enhanced endothelial migration, tube formation, and increased monocyte adhesion, demonstrating robust pro-angiogenic activity. In vivo, LNP@SDF-1α transfected HUVECs showed enhanced angiogenic capacity in a murine Matrigel plug model. Furthermore, in a mouse hindlimb ischemia model, intramuscular injection of LNP@SDF-1α into ischemic limbs accelerated blood flow recovery, as assessed by laser speckle contrast imaging. Immunofluorescence staining revealed a marked increase in capillary and arteriole densities in treated tissues. Angiogenic protein profiling demonstrated an upregulation of pro-angiogenic factors, including VEGF and Ang-1, and a downregulation of anti-angiogenic factors. No significant toxicity was observed in major organs, indicating the safety of this approach. Our study demonstrates that SDF-1α mRNA therapy, delivered via DOPE-LNPs, significantly promotes vascular regeneration in ischemic tissues by enhancing angiogenesis and arteriogenesis, thereby restoring blood perfusion. This approach presents a promising therapeutic option for PAD and suggests broader applications of mRNA-based therapies for ischemic diseases.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09979-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The VEGF decoy receptor soluble Fms-like tyrosine kinase 1 binds to macrophages","authors":"Cleo C. L. van Aanhold,&nbsp;Qing Yong,&nbsp;Lisa Landman,&nbsp;Samiksha Sardana,&nbsp;Anouk B. Bouwmeester,&nbsp;Kyra L. Dijkstra,&nbsp;Ron Wolterbeek,&nbsp;Hailiang Mei,&nbsp;Rayman T. N. Tjokrodirijo,&nbsp;Arnoud H. de Ru,&nbsp;Peter A. van Veelen,&nbsp;Jan A. Bruijn,&nbsp;Cees van Kooten,&nbsp;Hans J. Baelde","doi":"10.1007/s10456-025-09980-w","DOIUrl":"10.1007/s10456-025-09980-w","url":null,"abstract":"<div><h3>Background</h3><p>Soluble Fms-like Tyrosine kinase-1 (sFLT1) is a native inhibitor of VEGF, best known for its antiangiogenic effects in preeclampsia. sFLT1 also reduces chronic inflammation and promotes tissue repair. In experimental diabetic nephropathy, we previously found that sFLT1 ameliorates kidney fibrosis and reduces the infiltration of macrophages. How sFLT1 regulates inflammation is still incompletely understood. Based on the direct association of sFLT1 with various cell types, we here studied whether sFLT1 interacts with macrophages to modulate inflammation.</p><h3>Methods</h3><p>Using various macrophage cell lines, sFLT1 cell surface binding was detected with flow cytometry. Enzyme studies, mass spectrometry and RNAseq were employed to identify potential sFLT1 cell surface interactors and effects of sFLT1 on macrophage signaling.</p><h3>Results</h3><p>Soluble FLT1 binds to primary macrophages, THP-1 and RAW264.7 macrophages in vitro. Alternative activation with IL-4 increases sFLT1 binding in THP-1 macrophages, whereas proinflammatory activation with IFN-γ and LPS decreases binding. Binding of sFLT1 depends on heparan sulphates, and colocalizes with the membrane heparin sulfate proteoglycan neuropilin-1. Incubation with sFLT1 reduces the gene expression of chemokine receptors.</p><h3>Conclusion</h3><p>Our results show that sFLT1, while typically associated with angiogenesis, also directly interacts with macrophages. Alternative activation of macrophages by IL-4 strongly increases binding of sFLT1 to the cell surface membrane, possibly via the VEGF co-receptor neuropilin-1. Considering sFLT1’s anti-inflammatory effects in animal studies, our findings indicate a novel function for sFLT1 to directly control anti-inflammatory macrophage function. </p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09980-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixed lineage kinase (MLK) controls tumor development and angiogenesis","authors":"Shashi Kant,&nbsp;Amada D. Caliz,&nbsp;Hyung-Jin Yoo,&nbsp;Gaganpreet Kaur,&nbsp;Heather Learnard,&nbsp;Hassan A. Khalil,&nbsp;Roger J. Davis,&nbsp;John F. Jr. Keaney","doi":"10.1007/s10456-025-09978-4","DOIUrl":"10.1007/s10456-025-09978-4","url":null,"abstract":"<div><p>Cancer is among the leading causes of death in the USA and worldwide. Solid tumors require the formation of new blood vessels (angiogenesis) for their growth. The endothelium plays a crucial role in angiogenesis and tumor progression. Hypoxic stress generated by tumors can activate stress kinases such as mixed lineage kinases (MLKs). Publicly available datasets on lung adenocarcinoma, along with our experimental findings, indicate that MLK2 and MLK3 are expressed in human lung tumors. In this study, using three distinct mouse models of tumor development, we demonstrated that MLK2 (<i>MAP3K10</i>) and MLK3 (<i>MAP3K11</i>) are essential for tumor growth and angiogenesis. Furthermore, MLK2 and MLK3 are highly expressed in the endothelium and are necessary for endothelial proliferation, migration, and angiogenesis. In the endothelium, MLKs regulate the expression of angiogenic growth factors and metalloproteinases, including <i>Pgf</i>,<i> Vegfa</i>,<i> Angptl4</i>,<i> Adam8</i>,<i> and Mmp9</i>. Additionally, the MLK family of kinases acts through the long noncoding RNA (lncRNA) <i>H19</i> to control the expression of these pro-angiogenic factors in the endothelium. Collectively, these findings suggest that the MLK-H19 axis coordinates endothelial function, angiogenesis, and tumor growth.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"G protein-coupled receptor GPR182 negatively regulates sprouting angiogenesis via modulating CXCL12-CXCR4 axis signaling","authors":"Changsheng Chen,&nbsp;Wei Liu,&nbsp;Fang Yuan,&nbsp;Xiaoning Wang,&nbsp;Xi Xu,&nbsp;Chang Chun Ling,&nbsp;Xiaojuan Ge,&nbsp;Xiaozhong Shen,&nbsp;Bowen Li,&nbsp;Yuqian Shen,&nbsp;Dong Liu","doi":"10.1007/s10456-025-09977-5","DOIUrl":"10.1007/s10456-025-09977-5","url":null,"abstract":"<div><p>Angiogenesis is a critical process for tumor progression, regulated by various signaling pathways. Although antiangiogenic therapies targeting the VEGF pathway have shown potential, their effectiveness is inconsistent across different tumor types. GPR182, an endothelial cell-specific G protein-coupled receptor, is frequently downregulated in hypervascular tumors, but its specific role in angiogenesis has not been well defined. Our study reveals that GPR182 expression is markedly reduced in hepatocellular carcinoma (HCC) and inversely correlates with CD31, a pan-endothelial marker. In zebrafish embryos, Gpr182 deficiency resulted in enhanced angiogenic sprouting and hypervascularization, and GPR182-deficient human umbilical vein endothelial cells (HUVECs) showed increased migration and proliferation. At the molecular level, GPR182 acts as a decoy receptor, binding CXCL12 and regulating its gradient, which in turn suppresses CXCR4-mediated angiogenesis. The pharmacological blockade of CXCR4 with AMD3100 corrected the abnormal angiogenic phenotype in Gpr182-deficient zebrafish embryos and in the livers of a zebrafish HCC model. This work uncovers GPR182 as a negative regulator of angiogenesis, a key process in tumor growth and metastasis, and proposes that targeting GPR182 may offer a novel therapeutic approach for antiangiogenic strategies in cancer treatment.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 3","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09977-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ApoM-bound S1P acts via endothelial S1PR1 to suppress choroidal neovascularization and vascular leakage","authors":"Bongnam Jung,&nbsp;Hitomi Yagi,&nbsp;Andrew Kuo,&nbsp;Tim F. Dorweiler,&nbsp;Masanori Aikawa,&nbsp;Taku Kasai,&nbsp;Sasha A. Singh,&nbsp;Andrew J. Dannenberg,&nbsp;Zhongjie Fu,&nbsp;Colin Niaudet,&nbsp;Lois E. H. Smith,&nbsp;Timothy Hla","doi":"10.1007/s10456-025-09975-7","DOIUrl":"10.1007/s10456-025-09975-7","url":null,"abstract":"<div><p>Neovascular age-related macular degeneration (nAMD) is a major cause of vision loss worldwide. Current standard of care is repetitive intraocular injections of vascular endothelial growth factor (VEGF) inhibitors, although responses may be partial and non-durable. We report that circulating sphingosine 1-phosphate (S1P) carried by apolipoprotein M (ApoM) acts through the endothelial S1P receptor 1 (S1PR1) to suppress choroidal neovascularization (CNV) in mouse laser-induced CNV, modeling nAMD. In humans, low plasma ApoM levels were associated with increased choroidal and retinal pathology. Additionally, endothelial <i>S1pr1</i> knockout and overexpressing transgenic mice showed increased and reduced CNV lesion size, respectively. Systemic administration of ApoM-Fc, an engineered S1P chaperone protein, not only attenuated CNV to an equivalent degree as anti-VEGF antibody treatment but also suppressed pathological vascular leakage. We suggest that modulating circulating ApoM-bound S1P action on endothelial S1PR1 provides a novel therapeutic strategy to treat nAMD.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10456-025-09975-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial USP11 drives VEGFR2 signaling and angiogenesis via PRDX2/c-MYC axis","authors":"Can Chen,&nbsp;Zilong Chen,&nbsp;Ruijie Luo,&nbsp;Wanheng Tu,&nbsp;Minwen Long,&nbsp;Minglu Liang,&nbsp;Min Cheng,&nbsp;Zhaohui Wang,&nbsp;Kai Huang,&nbsp;Cheng Wang","doi":"10.1007/s10456-025-09976-6","DOIUrl":"10.1007/s10456-025-09976-6","url":null,"abstract":"<div><p>Angiogenesis is a crucial component of various physiological and pathological processes, including embryonic development, ischemic diseases, and tumor progression. Recent studies have highlighted the importance of ubiquitinases in angiogenesis. In this study, we utilized RNA sequencing data of the mouse retinal development model from the GEO database to identify the potential proangiogenic deubiquitinases and found USP11 was significantly upregulated. Although USP11 is known to regulate cell survival, DNA repair, and oxidative stress in cancers and ischemic conditions, its direct role in endothelial angiogenesis remains poorly understood. Here, we demonstrated that USP11 expression correlates with key pro-angiogenic genes and is significantly upregulated at both mRNA and protein levels in VEGF-treated human umbilical vein endothelial cells (HUVECs). USP11 knockout markedly inhibited angiogenesis both in vivo and in vitro, whereas USP11 overexpression promoted angiogenesis. Mechanistically, USP11 binds to PRDX2, facilitating the removal of its K63-linked polyubiquitination, which promotes its translocation into the nucleus. This facilitates the concurrent nuclear translocation of c-MYC, a PRDX2 interactor, which subsequently enhances the transcription of <i>KDR</i> (encoding VEGFR2) and activates the VEGFR2 signaling pathway. Our findings suggest that USP11 promotes angiogenesis by upregulating VEGFR2 expression through the PRDX2/c-MYC pathway, indicating that USP11 could serve as a potential target for clinical interventions in angiogenesis-related diseases.</p></div>","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microvascular and cerebrovascular alterations in Raynaud’s phenomenon and fibromyalgia","authors":"Angelo Nigro","doi":"10.1007/s10456-025-09974-8","DOIUrl":"10.1007/s10456-025-09974-8","url":null,"abstract":"","PeriodicalId":7886,"journal":{"name":"Angiogenesis","volume":"28 2","pages":""},"PeriodicalIF":9.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}