{"title":"Fatty acid exchange of phospholipids in membranes of rat heart","authors":"Ulrich Stoll","doi":"10.1016/S0003-5017(99)80006-3","DOIUrl":"10.1016/S0003-5017(99)80006-3","url":null,"abstract":"<div><p>Incorporation of <sup>14</sup>C fatty acids in phospholipids of plasma membranes and sarcoplasmic reticulum of rat heart was studied. Mainly phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were labelled. Our studies showed that the incorporation of unsaturated fatty acids (oleic and linoleic acid) was higher than for saturated fatty acids (palmitic and stearic acid). The range of uptake was between 0.2 and 2.2 nmol·mg<sup>−1</sup> protein·h<sup>−1</sup> and 0.5–7.4 nmol·μgatom<sup>−1</sup> P<sub>1</sub>·h<sup>−1</sup>, respectively. Uptake of activity in individual phospholipids (measured after separation on TLC) was calculated as percentage of total activity. Incorporation in phosphatidylcholine was higher than in phosphatidylethanolamine. Phosphatidylcholine showed an increasing sequence for the following fatty acids: C<sub>18:0</sub> < C<sub>16:0</sub> < C<sub>18:0</sub> < C<sub>18:2</sub>. However, phosphatidylethanolamine showed a decreasing sequence for the incorporation of the same fatty acids. Labelling of PC was always greater than for PE, except for stearic acid which was better incorporated into phosphatidylethanolamine. Uptake of the same fatty acid into phospholipids of sarcoplasmic reticulum was always higher than uptake into plasma membranes. As incorporation of fatty acids bound to albumin was studied in isolated membranes of rat heart, the addition of ATP and CoASH was an absolute requirement.</p></div>","PeriodicalId":78387,"journal":{"name":"L' Annee biologique","volume":"38 1","pages":"Pages 17-26"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0003-5017(99)80006-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116351926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}