Renal physiology最新文献_第6页

Interaction between laminin, fibronectin and the light chain of the H2 complex. Attachment of glomerular cells to basement membranes by linking the endoskeleton to the exoskeleton by a transmembrane protein. 层粘连蛋白、纤维连接蛋白与H2复合物轻链的相互作用。通过跨膜蛋白连接内骨骼和外骨骼，肾小球细胞附着在基底膜上。

Renal physiology Pub Date : 1986-01-01

G Lubec

{"title":"Interaction between laminin, fibronectin and the light chain of the H2 complex. Attachment of glomerular cells to basement membranes by linking the endoskeleton to the exoskeleton by a transmembrane protein.","authors":"G Lubec","doi":"","DOIUrl":"","url":null,"abstract":"At present the detailed mechanism for transmembrane interactions is not known and a protein linked to the endoskeleton as well as to the exoskeleton has not been described as yet. The H2 complex, a transmembrane protein, consists of heavy and light chains, the latter is named beta-2-microglobulin. In order to look for an association of beta-2-microglobulin with an exoskeleton protein, we examined the extracellular matrix proteins, collagen type I, type IV, fibronectin, amyloid P, the solubilized glomerular basement membrane and laminin in respect to their interaction with the light chain. The heavy chain is known to bind strongly to the endoskeleton protein actin. Only laminin and the glomerular basement membrane bound firmly to the beta-2-microglobulin; 3 M urea was necessary to dissociate the formed complex. Incubation with beta-2-microglobulin antibody prevented binding of beta-2-microglobulin to laminin and the glomerular basement membrane on affinity chromatography columns. Antiserum to the glomerular basement membrane in turn prevented binding of beta-2-microglobulin to the glomerular basement membrane, whereas antibodies against the basement membrane collagen type IV failed to inhibit this binding to the glomerular basement membrane. Beta-2-microglobulin also bound to fibronectin but this complex was dissociated with 1 M urea. In a rosette assay beta-2-microglobulin antibody and antiserum to the glomerular basement membrane reduced attachment of glomerular cells to beads coupled with laminin and solubilized glomerular cells to beads coupled with laminin and solubilized glomerular basement membrane.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 1-2","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14637193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The lysosomal distribution of cathepsin B in the rat kidney cortex. 组织蛋白酶B在大鼠肾皮质溶酶体的分布。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173103

K J Andersen, M Dobrota

{"title":"The lysosomal distribution of cathepsin B in the rat kidney cortex.","authors":"K J Andersen, M Dobrota","doi":"10.1159/000173103","DOIUrl":"https://doi.org/10.1159/000173103","url":null,"abstract":"Subcellular distribution of cathepsin B following subfractionation of the kidney cortex mitochondrial/lysosomal fraction by rate sedimentation indicates that this enzyme is mainly associated with the large, fast sedimenting lysosomes (protein droplets). A small proportion of cathepsin B is also present in the small lysosomes which cosediment with mitochondria, peroxisomes, and brush border and other large membrane vesicles. Amongst this broad spectrum of small lysosomes the distribution of cathepsin B, together with other acid hydrolases is associated with the more rapidly sedimenting lysosomes whilst cathepsin D differs in being associated with the slowest sedimenting lysosomes. Equilibrium banding in sucrose gradients shows the large lysosomes band at a density of 1.235 g/ml and that the small lysosomes have two distinct populations at densities 1.20 and 1.235 g/ml. Cathepsin B (and also cathepsin D and acid ribonuclease) appears to be associated only with lysosomes of high density. The various other acid hydrolases assayed are found in all the lysosomal populations. Small and large lysosomes of high density are very rich in a number of proteinases and therefore most probably represent lysosomal populations involved in the catabolism of proteins taken up from the glomerular filtrate.","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 6","pages":"375-83"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000173103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14723283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Renal tubular uptake of protein: effect of pH. 肾小管蛋白摄取:pH的影响。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173080

E I Christensen, T Bjerke

引用次数: 11

Whole kidney response to reduced arterial pressure during converting enzyme inhibition in the rat. 大鼠转换酶抑制期间全肾对降低动脉压的反应。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173093

A. Göransson, B. Isaksson, M. Sjöquist

{"title":"Whole kidney response to reduced arterial pressure during converting enzyme inhibition in the rat.","authors":"A. Göransson, B. Isaksson, M. Sjöquist","doi":"10.1159/000173093","DOIUrl":"https://doi.org/10.1159/000173093","url":null,"abstract":"Autoregulatory efficiency of renal blood flow (RBF) and glomerular filtration rate (GFR) was evaluated in male Sprague-Dawley rats during interference with the renin-angiotensin system by a converting enzyme inhibitor (CEI), captopril (3 mg X h-1 X kg-1 BW). RBF and GFR were approximately 25 (p less than 0.01) and 20% (p less than 0.02) higher, respectively, in rats infused with CEI than in control rats at spontaneous renal arterial pressure (RAP). A reduction of RAP to 100 mm Hg (within the autoregulatory range) resulted in effective autoregulation of GFR and RBF in control rats. In rats given CEI, however, the autoregulation of GFR was markedly impaired. GFR decreased by 35% (p less than 0.001), while RBF remained relatively unchanged. This caused the filtration fraction to decrease from 0.33 +/- 0.01 to 0.29 +/- 0.01 (p less than 0.001). RAP had a consistent effect on the urine flow rate, even though both GFR and RBF were well autoregulated in control rats. No significant decrease in electrolyte excretion was detected within the autoregulatory range in control rats, but during converting enzyme blockade this excretion decreased progressively as RAP was reduced, and the decrease correlated well to the reduction in GFR. In summary, these results suggest that the renin-angiotensin system plays an important intrarenal role in the autoregulation of GFR, probably through an efferent arteriolar mechanism. Furthermore, it is demonstrated that the contralateral kidney efficiently compensates in urinary electrolyte excretion for an acute unilateral reduction of RAP.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 5 1","pages":"287-301"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000173093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65092673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Urinary kinin excretion following alterations of vasopressin levels in man and rat. 人与大鼠抗利尿激素水平变化后尿激肽排泄。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173094

A. Náray-Fejes-Tóth, G. Fejes-Tóth

引用次数: 4

Renal high-molecular-weight renin: unusual formation in the aged stroke-prone spontaneously hypertensive rats. 老年卒中易发自发性高血压大鼠肾脏高分子量肾素的异常形成。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173082

S Itoh, M Tanaka, F Ikemoto, K Yamamoto, N Morita, K Okamoto

{"title":"Renal high-molecular-weight renin: unusual formation in the aged stroke-prone spontaneously hypertensive rats.","authors":"S Itoh, M Tanaka, F Ikemoto, K Yamamoto, N Morita, K Okamoto","doi":"10.1159/000173082","DOIUrl":"https://doi.org/10.1159/000173082","url":null,"abstract":"A high-molecular-weight renin (HMWR) was detected in the plasma (molecular weight 50,600) and renal cortical homogenate (molecular weight 57,000) of 25-week-old male stroke-prone spontaneously hypertensive rats (SHRSP), in contrast to the renin with a molecular weight of 38,000 in normotensive Wistar Kyoto rats (WKY) and 5-week-old SHRSP. However, renin granules contained only the renin with a molecular weight of 38,000, indicating that the renal HMWR is not a native form but is probably a renin/renin-binding protein complex. Such HMWR was not produced when the renal cortex was homogenized with an equal amount of renal cortex of WKY. Further, the HMWR of the aged SHRSP was converted into the 38,000-dalton renin, when incubated with the extract of renal cortex of WKY. Thus, the existence of a renal cortical substance that converts the renal HMWR into the 38,000-dalton renin was evidenced. This substance was fractionated with DEAE-cellulose and was characterized as a putative SH enzyme. We conclude that a deficiency in the HMWR-converting substance may be attributed to the unusual formation of HMWR in the aged SHRSP.","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 3","pages":"177-86"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000173082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14651843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sodium chloride, water and urea handling in the rat renal medulla: a computer simulation. 大鼠肾髓质中氯化钠、水和尿素处理的计算机模拟。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173087

G L Barrett, J S Packer, J M Davis

引用次数: 3

Renin status of the afferent arteriole and ultrastructure of the juxtaglomerular apparatus in 'superficial' juxtamedullary nephrons from rats. 大鼠髓旁浅浅肾元传入小动脉肾素状态及肾小球旁器官超微结构。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173100

D Casellas, R Taugner

{"title":"Renin status of the afferent arteriole and ultrastructure of the juxtaglomerular apparatus in 'superficial' juxtamedullary nephrons from rats.","authors":"D Casellas, R Taugner","doi":"10.1159/000173100","DOIUrl":"https://doi.org/10.1159/000173100","url":null,"abstract":"The present light (LM) and transmission electron microscopic (TEM) studies were carried out to further document the anatomy of the 'superficial' juxtamedullary nephrons (SJMNs) located on the inside cortical surface of the rat kidney. TEM revealed that SJMNs possess all vascular and tubular cell types constituting the juxtaglomerular apparatus (JGA) in typical cortical and juxtamedullary nephrons (JMNs). Proximal to the glomeruli, epithelioid cells filled with secretory granules predominated in the media of afferent arterioles. The presence of renin in the granules was immunocytochemically demonstrated by the protein A-gold method. Further upstream from the glomeruli, epithelioid cells alternated with plain smooth muscle cells. The use of renin and angiotensin II antisera revealed similar arteriolar distributions of renin and angiotensin II positive cells in SJMNs as well as in typical JMNs. Numerous nerve terminals were found along afferent and efferent arterioles, suggesting a dense innervation of these vessels. The distribution of renin-containing (i.e., epithelioid) cells in the preglomerular arterioles was assessed in various nephron populations by LM using the PAP method and renin antiserum. In SJMNs, most renin-positive cells were found in the vicinity of the JGA along a mean arteriolar length of 35 +/- 3 micron (range 7-107 micron). In JMNs, renin-positive cells had a similar distribution along a mean arteriolar length of 35 +/- 1 micron. Scattered renin-positive cells were observed up to a maximal arteriolar length of 173 and 238 micron in SJMNs and JMNs, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 6","pages":"348-56"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000173100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14427773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Renal autoregulatory efficiency during angiotensin-converting enzyme inhibition in dogs on a low sodium diet. 低钠饮食犬血管紧张素转换酶抑制期间肾脏自身调节效率。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173072

L Rosivall, P Youngblood, L G Navar

{"title":"Renal autoregulatory efficiency during angiotensin-converting enzyme inhibition in dogs on a low sodium diet.","authors":"L Rosivall, P Youngblood, L G Navar","doi":"10.1159/000173072","DOIUrl":"https://doi.org/10.1159/000173072","url":null,"abstract":"Autoregulatory efficiency of renal blood flow (RBF) and glomerular filtration rate (GFR) was evaluated in 12 anesthetized dogs that had been maintained on low-sodium diet during control conditions and following infusion of an angiotensin-converting enzyme inhibitor (captopril). Converting enzyme inhibition (CEI) decreased systemic blood pressure by 15.5 +/- 3.5%, increased RBF by 36.3 +/- 6.5%, and increased GFR by 25.9 +/- 10.7%. In response to reductions in renal arterial pressure, RBF was efficiently autoregulated and did not change significantly until the 89- to 75-mm Hg range during the control period and the 74- to 54-mm Hg range during CEI. Overall GFR autoregulatory efficiency was generally well maintained during CEI; however, evaluation of the coupled autoregulatory efficiency of RBF and GFR indicated that during angiotensin blockade, there was a greater incidence of a dissociation between RBF and GFR autoregulatory efficiency. Six of the 12 dogs showed reduced GFR autoregulatory efficiency at renal arterial pressures where RBF was still well maintained. Thus, while the data indicate that blockade of the renin-angiotensin system does not abolish the basic capability of the kidney to autoregulate either RBF or GFR efficiently, more subtle influences on the coupling of RBF and GFR autoregulatory efficiency were observed at the lower level of the autoregulatory range.","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 1-2","pages":"18-28"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000173072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14142896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Biphasic alteration of renin-angiotensin-aldosterone system in streptozotocin-diabetic rats. 链脲佐菌素-糖尿病大鼠肾素-血管紧张素-醛固酮系统的双相改变。

Renal physiology Pub Date : 1986-01-01 DOI: 10.1159/000173083

R Kikkawa, E Kitamura, Y Fujiwara, M Haneda, Y Shigeta

{"title":"Biphasic alteration of renin-angiotensin-aldosterone system in streptozotocin-diabetic rats.","authors":"R Kikkawa, E Kitamura, Y Fujiwara, M Haneda, Y Shigeta","doi":"10.1159/000173083","DOIUrl":"https://doi.org/10.1159/000173083","url":null,"abstract":"The alteration of renin-angiotensin-aldosterone system caused by diabetes mellitus was studied in streptozotocin-diabetic rats. The plasma renin activity (PRA), and plasma levels of angiotensin II (A II) and aldosterone (PAC) were measured in diabetic and age-matched control rats in 1, 2, 4 and 8 weeks after the intravenous injection of streptozotocin (50 mg/kg body weight). Diabetic rats showed the marked hyperglycemia persistently throughout the experimental period. On 1st week PRA, A II and PAC were significantly increased, and A II and PAC were also significantly elevated on 2nd week in diabetic rats compared with control rats. However, on 4th and 8th weeks PRA, A II and PAC in diabetic rats were significantly lower than those of control rats. Hematocrit values in diabetic rats were elevated on 1st week, normalized on 2nd and 4th weeks and then decreased on 8th week. These results may suggest that the hyperglycemia causes a biphasic alteration of renin-angiotensin-aldosterone system, i.e., early stimulated state due to volume depletion and later suppressed state due to volume expansion.","PeriodicalId":77779,"journal":{"name":"Renal physiology","volume":"9 3","pages":"187-92"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000173083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14651844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40