Neurochemical pathology最新文献_第8页

Effects of subperineurial injections of very-long-chain and medium-chain fatty acids into rat sciatic nerve. 大鼠坐骨神经神经周下注射超长链和中链脂肪酸的影响。

Neurochemical pathology Pub Date : 1986-08-01 DOI: 10.1007/BF03028037

N C Sacktor, J Griffin, A B Moser, H W Moser

引用次数: 3

Further probes into the molecular sites of damage to cerebral adenylate cyclase following postischemic reperfusion. 进一步探讨脑缺血再灌注后脑腺苷酸环化酶损伤的分子位点。

Neurochemical pathology Pub Date : 1986-08-01 DOI: 10.1007/BF03028033

G C Palmer, D J Jones, S J Palmer, B C Christie-Pope, L Poulakos

{"title":"Further probes into the molecular sites of damage to cerebral adenylate cyclase following postischemic reperfusion.","authors":"G C Palmer, D J Jones, S J Palmer, B C Christie-Pope, L Poulakos","doi":"10.1007/BF03028033","DOIUrl":"https://doi.org/10.1007/BF03028033","url":null,"abstract":"A variety of pharmacological agents were used as experimental probes to determine with greater precision the site(s) of damage to cerebral adenylate cyclase as a consequence of postischemic reperfusion in the gerbil. A paradigm of 60-min bilateral ischemia followed by 40-min reperfusion results in a decreased sensitivity of the catalytic site of adenylate cyclase to Mn2+. Likewise, the GTP-transducer site (guanine nucleotide regulatory or G protein) revealed depressed responses to GTP in the absence or presence of norepinephrine, dopamine agonists, substance P, yohimbine, and cholera and pertussis toxins. Moreover, a crude preparation of GTPase disclosed that damage elicited by postischemic reperfusion was directed to the higher-affinity form of this enzyme, which is associated with the overall function of the guanine nucleotide regulatory protein. Injury to adenylate cyclase was unrelated either to the ability of adrenergic ligands to bind to associated receptor sites or to the capacity of the brain to generate visual evoked potentials in response to visual stimuli.","PeriodicalId":77753,"journal":{"name":"Neurochemical pathology","volume":"5 1","pages":"1-23"},"PeriodicalIF":0.0,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF03028033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14236709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Potential biochemical markers for infantile autism. 婴儿自闭症的潜在生化标记。

Neurochemical pathology Pub Date : 1986-08-01 DOI: 10.1007/BF03028036

P P Israngkun, H A Newman, S T Patel, V A Duruibe, H Abou-Issa

引用次数: 51

Rabbit brain lipids during short-term hyperthermia. 兔在短期高温下的脑脂质。

Neurochemical pathology Pub Date : 1986-06-01 DOI: 10.1007/BF02834355

K Domańska-Janik, Z Dabrowiecki, W Gordon-Majszak, J Strosznajder

{"title":"Rabbit brain lipids during short-term hyperthermia.","authors":"K Domańska-Janik, Z Dabrowiecki, W Gordon-Majszak, J Strosznajder","doi":"10.1007/BF02834355","DOIUrl":"https://doi.org/10.1007/BF02834355","url":null,"abstract":"Rabbits were subjected to 3 h of hyperthermia at 40 degrees C. The phospholipid content of the brain was unchanged, whereas the free fatty acids increased by about 73% over control levels. Hyperthermia also induced inhibition of fatty acid peroxidation processes. The last products of fatty acid peroxidation, the thiobarbituric acid reactive substances, diminished significantly during hyperthermia, whereas the level of the first intermediate of this pathway, the conjugated double bonds, remained unchanged. Simultaneously, the levels of lipid-soluble antioxidants decreased significantly. The content of free fatty acids, malondialdehyde, and lipid-soluble anti-oxidant returned toward control levels during 3 h of recovery. The content of gangliosides was 10-20% above control values in the groups of animals examined immediately and 3 h after hyperthermia. The ganglioside-specific enzymes, neuraminidase and sialyltransferase, both directed toward endogenous lipid substrates, were activated by hyperthermia, suggesting the stimulation of turnover of the gangliosides during the course of hyperthermia. Lipid alterations resulting from short-term hyperthermia may influence the physicochemical properties of neuronal membranes.","PeriodicalId":77753,"journal":{"name":"Neurochemical pathology","volume":"4 3","pages":"153-63"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02834355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14683962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain. 从冷冻大鼠和人脑制备代谢活性突触体的冷冻、储存和解冻条件的优化。

Neurochemical pathology Pub Date : 1986-06-01 DOI: 10.1007/BF02834357

P R Dodd, J A Hardy, E B Baig, A M Kidd, E D Bird, W E Watson, G A Johnston

{"title":"Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain.","authors":"P R Dodd, J A Hardy, E B Baig, A M Kidd, E D Bird, W E Watson, G A Johnston","doi":"10.1007/BF02834357","DOIUrl":"https://doi.org/10.1007/BF02834357","url":null,"abstract":"Samples of rat and human cerebral cortex were frozen, stored, and thawed under a variety of conditions to define further the optimal procedure for storing human brain samples for subsequent metabolic and functional studies that use incubated synaptosomes. Tissue samples were best preserved by immersing them in isotonic sucrose prior to slow freezing, but there was no advantage in first chopping up the material. High concentrations of sucrose, rather than exerting a cryoprotective effect, were detrimental to subsequent synaptosomal performance (oxygen uptake, K+ accumulation, stimulus-induced release of amino acid neurotransmitters). However, good activity was observed in preparations from rat brain frozen in the absence of fluid. This result was confirmed by studies on the uptake of gamma-aminobutyrate (GABA) into an osmotically sensitive compartment in synaptosomes prepared from frozen human autopsy material transshipped from the brain tissue collection (\"brain bank\") at Harvard Medical School, MA, USA, to Sydney, Australia. Although activity was slowly lost over a 3-mo period in rat tissue samples stored at -20 degrees C, there was little or no such loss at -70 degrees C.","PeriodicalId":77753,"journal":{"name":"Neurochemical pathology","volume":"4 3","pages":"177-98"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02834357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14683964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81

Brain lipids of a myelin-deficient rabbit mutant during development. 发育过程中髓磷脂缺陷兔突变体的脑脂质。

Neurochemical pathology Pub Date : 1986-06-01 DOI: 10.1007/BF02834354

K Domańska-Janik, H Wikiel, I Zelman, J Strosznajder

{"title":"Brain lipids of a myelin-deficient rabbit mutant during development.","authors":"K Domańska-Janik, H Wikiel, I Zelman, J Strosznajder","doi":"10.1007/BF02834354","DOIUrl":"https://doi.org/10.1007/BF02834354","url":null,"abstract":"The paralytic tremor (pt) rabbit, a neurological mutant, exhibits hypomyelination transmitted in X-linked recessive fashion. This rabbit mutant was used for regional lipid analyses of different brain structures during development. There was a significant decrease of myelin-specific lipids, particularly in the cerebroside and sulfatide in pt rabbits. The decrease of phospholipid and cholesterol was relevant to the total lipids depletion. The molar ratio of galactolipid to phospholipid decreased in the pt rabbit brain in each age group examined. The other lipids typical for myelin, such as ethanolamine glycerophospholipids, sphingomyelin, and GM1 ganglioside, were also diminished in the myelin-rich structures, but were not changed in the cortical gray matter of pt rabbits. In contrast, the total amount of gangliosides was near control levels and, therefore, in the mutant rabbits, the white matter and brain stem contained a higher proportion of lipid, as ganglioside, relative to the control animals. This result suggests that neuronal membranes were not involved in this pathology. The characteristic biochemical abnormalities exhibited in the pt rabbit suggest that a defect of oligodendroglial cell function is primarily responsible for the myelin abnormality.","PeriodicalId":77753,"journal":{"name":"Neurochemical pathology","volume":"4 3","pages":"135-51"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02834354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14683961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Diabetic rat serum has an increased capacity to inhibit brain microtubule formation in vitro. 糖尿病大鼠血清体外抑制脑微管形成的能力增强。

Neurochemical pathology Pub Date : 1986-06-01 DOI: 10.1007/BF02834356

W G McLean, S J Beahon, I F Casson

引用次数: 3

Cellular hypersensitivity to nervous antigens in Guillain-Barré syndrome. 格林-巴罗综合征中细胞对神经抗原的超敏反应。

Neurochemical pathology Pub Date : 1986-04-01 DOI: 10.1007/BF03160190

R Ohno, K Hamaguchi, K Nomura, T Negishi, M Suzuki, K Uyemura

引用次数: 6

Presence of glycoproteins containing the polylactosamine structure in brain and liver of GM1 gangliosidosis patients. Comparative study between clinical types I and II, using endo-beta-galactosidase enzyme. GM1神经节脂质沉积症患者脑和肝脏中含有多乳糖胺结构的糖蛋白的存在。临床ⅰ型与ⅱ型半乳糖苷内切酶的比较研究。

Neurochemical pathology Pub Date : 1986-04-01 DOI: 10.1007/BF03160189

B Berra, R De Gasperi, S Rapelli, S Okada, S C Li, Y T Li

{"title":"Presence of glycoproteins containing the polylactosamine structure in brain and liver of GM1 gangliosidosis patients. Comparative study between clinical types I and II, using endo-beta-galactosidase enzyme.","authors":"B Berra, R De Gasperi, S Rapelli, S Okada, S C Li, Y T Li","doi":"10.1007/BF03160189","DOIUrl":"https://doi.org/10.1007/BF03160189","url":null,"abstract":"The material derived from defective degradation of glycoproteins, which accumulates in brain and liver of a patient with GM1 gangliosidosis type I, was investigated, and the structure of the main storage compounds determined. For comparison, brain and liver of a patient with GM1 gangliosidosis type II were also analyzed. Analysis of the glycopeptides obtained after pronase digestion of the defatted residue indicates the storage of glycoprotein-like material in type I, but not in type II. Treatment with endo-beta-galactosidase showed that the stored material contained N-acetyllactosamine repeating units. Two major oligosaccharides, OS I and OS II, were isolated after the enzyme treatment, whose structures are: GlcNAc beta 1----3 Gal (OS I) and Gal beta l----4GlcNAc beta 1----3 Gal (OS II). Treatment with exo-beta-galactosidase transformed the trisaccharide OS II into the disaccharide OS I, indicating that the deficiency of beta-galactosidase in GM1 gangliosidosis type I, but not in type II, also affects glycoprotein catabolism, leading to the accumulation of glycopeptides containing terminal beta-galactosyl residues and N-acetyllactosamine repeating units. These results indicate the severe impairment in the catabolism of glycoconjugates with beta-linked galactose in type I, although this impairment is not as pronounced in type II.","PeriodicalId":77753,"journal":{"name":"Neurochemical pathology","volume":"4 2","pages":"107-17"},"PeriodicalIF":0.0,"publicationDate":"1986-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF03160189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14219732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Absence of myelin basic protein in an improved purified duck embryo rabies vaccine. 改良纯化鸭胚狂犬病疫苗中髓鞘碱性蛋白缺失。

Neurochemical pathology Pub Date : 1986-04-01 DOI: 10.1007/BF03160186

R Glück, J M Matthieu, A Wegmann, F Méan

引用次数: 10