Journal of free radicals in biology & medicine最新文献_第7页

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80014-9

引用次数: 0

Editor's note 编者按

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80028-9

引用次数: 0

Treatment of lymphocyte cultures with a hypoxanthine-xanthine oxidase system induces the formation of transferable clastogenic material 用次黄嘌呤-黄嘌呤氧化酶系统处理淋巴细胞培养诱导可转移的致裂物质的形成

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90029-7

Ingrid Emerit , Shahid H. Khan , Peter A. Cerutti

引用次数: 72

Iron-induced lipid peroxidation in spinal cord: Protection with mannitol and methylprednisolone 铁诱导的脊髓脂质过氧化:甘露醇和甲基强的松龙的保护作用

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90030-3

Douglas K. Anderson , Eugene D. Means

{"title":"Iron-induced lipid peroxidation in spinal cord: Protection with mannitol and methylprednisolone","authors":"Douglas K. Anderson , Eugene D. Means","doi":"10.1016/0748-5514(85)90030-3","DOIUrl":"10.1016/0748-5514(85)90030-3","url":null,"abstract":"<div><p>The ability of the free radical scavenger, mannitol, and the synthetic glucocorticoid, methylprednisolone sodium succinate (MPSS) to reverse the effects of iron catalyzed free radical induced lipid peroxidation was assessed in the feline spinal cord. Ferrous chloride (100 mM) was infused into the gray matter of lumbar spinal cord, the region frozen in situ, removed, and homogenates of the gray matter analyzed for activity of Na<sup>+</sup>, K<sup>+</sup>- ATPase and levels of malondialdehyde (MDA). ATPase activity had declined to approximately 30% of control by 2 h after FeCl<sub>2</sub> infusion and remained at this level through 24 h. Malondialdehyde values were elevated almost twofold at 2 h. Mannitol essentially reversed the effects of FeCl<sub>2</sub> infusion on Na<sup>+</sup>,K<sup>+</sup>-ATPase activity and MDA production. These results may implicate the hydroxyl radical (·OH), or an oxidizing species with ·OH-like reactivity, as the initiating radical species in this model of lipid peroxidation. Similarly, MPSS prevented the decline in spinal cord Na<sup>+</sup>, K<sup>+</sup>-ATPase activity and rise in MDA levels that were induced by FeCl<sub>2</sub> infusion. This demonstrated that at the dosage levels used in this study, MPSS was an effective antioxidant. This finding provides presumptive evidence suggesting that, at least in experimental animals, the effectiveness of MPSS in preventing the tissue necrosis and paralysis that is the sequelae of spinal cord trauma may reside, in part, in the capacity of this glucocorticord to quench peroxidative reactions in the injured tissue.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"1 1","pages":"Pages 59-64"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(85)90030-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14146652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 90

Superoxide radical scavenging ability of centrophenoxine and its salt dependence in vitro 正苯氧酚清除超氧自由基的能力及其体外盐依赖性

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90153-9

Imre Semsei, Imre Zs.-Nagy

{"title":"Superoxide radical scavenging ability of centrophenoxine and its salt dependence in vitro","authors":"Imre Semsei, Imre Zs.-Nagy","doi":"10.1016/0748-5514(85)90153-9","DOIUrl":"10.1016/0748-5514(85)90153-9","url":null,"abstract":"<div><p>The superoxide radical scavenging ability of centrophenoxine (CPH) and its components (dimethylaminoethanol = DMAE, <em>p</em>-chlorophenoxyacetic acid = PCPA) was studied in vitro using the method of pyrogallol autoxidation, cytochrome c reduction and photoxidation of <em>o</em>-dianisidine in salt free assay media and in the presence of increasing NaCl or KCl concentrations. The CPH proved to be a superoxide radical scavenger in all three systems used, however, the rate constant for this reaction was rather low (1.7 × 10<sup>2</sup> M<sup>−1</sup> s<sup>−1</sup>). This scavenging ability decreased linearly with increasing ionic strength. DMAE and PCPA behaved in a somewhat contradictory manner. The former proved to be a weak superoxide radical generating compound being partially sensitive to the ionic strength. The latter showed either superoxide radical scavenging or generating effects on various assays depending on the actual salt concentrations of the media. On the basis of the results one has to assume that the superoxide radical scavenger ability of CPH may hardly be responsible for the in vivo effects of this compound, therefore, its OH· radical scavenger reactions the rate constant of which is about 10<sup>9</sup> M<sup>−1</sup> s<sup>−1</sup> (See Ref. 28.) may be of much greater importance.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"1 5","pages":"Pages 403-408"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(85)90153-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14151019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Autocatalytic oxidation of hemoglobin induced by nitrite: Activation and chemical inhibition 亚硝酸盐诱导血红蛋白的自催化氧化:活化和化学抑制

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90019-4

Michael P. Doyle, James G. Herman, Russell L. Dykstra

{"title":"Autocatalytic oxidation of hemoglobin induced by nitrite: Activation and chemical inhibition","authors":"Michael P. Doyle, James G. Herman, Russell L. Dykstra","doi":"10.1016/0748-5514(85)90019-4","DOIUrl":"10.1016/0748-5514(85)90019-4","url":null,"abstract":"<div><p>The nitrite ion is a direct causative agent for methomoglobinemia. Oxidation of hemoglobin to methemoglobin under aerobic conditionsis induced by nitrite, catalyzed by methomoglobin in the presence of hydrogen peroxide, and inhibited by chemical reagents ranging from cysteine and ascorbic acid to sulfite. The stoichiometry of nitrate production is dependent on the initial <span><math><mtext>[</mtext><mtext>NO</mtext><msub><mi></mi><mn>2</mn></msub><msup><mi></mi><mn>−</mn></msup><mtext>]</mtext><mtext>[</mtext><mtext>HbO</mtext><msub><mi></mi><mn>2</mn></msub><mtext>]</mtext></math></span> ratio, but reaches a limiting value of <span><math><mtext>1:1[</mtext><mtext>NO</mtext><msub><mi></mi><mn>3</mn></msub><msup><mi></mi><mn>−</mn></msup><mtext>]: [</mtext><mtext>Hb</mtext><msup><mi></mi><mn>+</mn></msup><mtext> </mtext><mtext>when</mtext><mtext> </mtext><mtext>[</mtext><mtext>NO</mtext><mtext>]</mtext><msub><mi></mi><mn>2</mn></msub><msup><mi></mi><mn>−</mn></msup><mtext>]</mtext><mtext>[</mtext><mtext>HbO</mtext><msub><mi></mi><mn>2</mn></msub><mtext>]</mtext><mtext> > 8</mtext></math></span>. Ascorbic acid is an exceptionally effective inhibitor for the autocatalytic oxidation, but its use does not affect the stoichiometry of nitrate formation. Sulfite reduces nitrate production to a level that is half that observed in its absence. These chemical inhibitors act upon the rapid autocatalytic stage for hemoglobin oxidation, but they do not influence the slow direct oxidation of hemoglobin by nitrite. The autocatalytic stage for hemoglobin oxidation results from nitrogen dioxide formed from nitrite through the peroxidase activity of methemoglobin. Peroxide and methemoglobin are formed during the initiation stage by electron transfer from nitrite that is kinetically first order in oxyhemoglobin and in nitrite.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"1 2","pages":"Pages 145-153"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(85)90019-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14951597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 78

Mechanisms of lipid peroxidation 脂质过氧化的机制

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90011-X

Albert W. Girotti

引用次数: 516

Lipid peroxidation products and clastogenic material in culture media of human leukocytes exposed to the tumor promoter phorbol-myristate-acetate 肿瘤启动子phorboll -肉豆蔻酸酯乙酸暴露于人白细胞培养基中的脂质过氧化产物和致裂物质

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90159-X

Shahid H. Khan, Ingrid Emerit

{"title":"Lipid peroxidation products and clastogenic material in culture media of human leukocytes exposed to the tumor promoter phorbol-myristate-acetate","authors":"Shahid H. Khan, Ingrid Emerit","doi":"10.1016/0748-5514(85)90159-X","DOIUrl":"10.1016/0748-5514(85)90159-X","url":null,"abstract":"<div><p>The chromosome-damaging effect of PMA in blood cultures is mediated by secondary products which are formed by the cells in response to the interaction with this tumor promoter. Since this effect could be influenced by antioxidant enzymes and by inhibitors of arachidonic acid metabolism, the present study was undertaken in order to determine whether the formation of these clastogenic substances is concomitant with the formation of AA metabolites and other lipid peroxidation products. Besides the clastogenic effect of ethyl-acetate extracts, the similarities of cytogenetic and biochemical results (conjugated dienes and TBA-reactive material) obtained for the influence of other blood cells than lymphocytes in the culture system, the importance of PHA stimulation and the protective effect of antioxidant enzymes were arguments in favour of a causal relationship between chromosome damage and lipid peroxidation (enzymatic or nonenzymatic). If AA release from membrane phospholipids was prevented by inhibition of phospholipase A<sub>2</sub>, neither conjugated dienes nor TBA-reactive material were found, and chromosome damage was reduced considerably. However, the results obtained with inhibitors of the cyclo- and lipoxygenase pathway were not conclusive, and discrepancies were also observed in the time course of appearance of clastogenic material and lipid peroxidation products.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"1 5","pages":"Pages 443-449"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(85)90159-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15053760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Free radicals in ischemic myocardial injury 自由基在缺血性心肌损伤中的作用

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90013-3

Steven W. Werns M.D. , Michael J. Shea M.D. , Benedict R. Lucchesi M.D., Ph.D.

{"title":"Free radicals in ischemic myocardial injury","authors":"Steven W. Werns M.D. , Michael J. Shea M.D. , Benedict R. Lucchesi M.D., Ph.D.","doi":"10.1016/0748-5514(85)90013-3","DOIUrl":"10.1016/0748-5514(85)90013-3","url":null,"abstract":"<div><p>Myrocardial ischemia causes release of chemotactic factors, migration of neutrophils, peroxidation of lipids, and depletion of free radical scavengers. The invading neutrophils may injure the myrocardial vasculature and sarcolemma by generating oxygen free radicals. Several agents that affect neutrophils or oxygen radicals were evaluated in a canine model of regional myocardial ischemia and reperfusion. Anesthetized dogs underwent occlusion and reperfusion of the left circumflex coronary artery. Infarct zone, area at risk infarction, and total left ventricle were quantified by gravimetric and planimetric analysis. Limitation of infarct size by ibuprofen was associated with marked suppression of leukocyte accumulation within the ischemic myocardium. Neutrophil depletion by antiserum resulted in similar reductions of infarct size and was accompanied by a reduction in leukocyte infiltration. A combination of oxygen radical scaverngers, superoxide dismutase plus catalase, decreased myocardial injury whether infusion began before occlusion or 75 min after occlusion. None of the treatments significantly altered hemodynamic indices of myocardial oxygen demand. Reduction of infarct size by ibuprofen, neutrophil antiserum, and free radical scavengers indicates that neutrophils and oxygen radicals participate in producing the irreversible damage to the myocardium during ischemia and reperfusion.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"1 2","pages":"Pages 103-110"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(85)90013-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15053962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 86

Myoglobin-catalyzed hydrogen peroxide dependent arachidonic acid peroxidation 肌红蛋白催化过氧化氢依赖花生四烯酸过氧化

Journal of free radicals in biology & medicine Pub Date : 1985-01-01 DOI: 10.1016/0748-5514(85)90122-9

Matthew B. Grisham

{"title":"Myoglobin-catalyzed hydrogen peroxide dependent arachidonic acid peroxidation","authors":"Matthew B. Grisham","doi":"10.1016/0748-5514(85)90122-9","DOIUrl":"10.1016/0748-5514(85)90122-9","url":null,"abstract":"<div><p>Hemeproteins promote lipid hydroperoxide-dependent lipid peroxidation in vitro. Only recently have studies demonstrated that certain hemeproteins peroxidize lipids in a lipid-hydroperoxide-independent manner. To understand fully the interaction between reactive oxygen metabolites, myoglobin and lipid, we investigate the possibility that myoglobin may use xanthine oxidase-generated superoxide and/or hydrogen peroxide to catalyze peroxidation of a polyunsaturated fatty acid. Our studies demonstrate that myoglobin, in the presence of hypoxanthine and xanthine oxidase, catalyze the peroxidation of arachidonic acid. Oxy (ferrous) myoglobin appears to be the most effective catalyst for arachidonic acid peroxidation when compared to metmyglobin, hemoglobin, or ADP-iron chelates. Inhibition studies reveal that myoglobin uses hydrogen peroxide, not superoxide to form either an oxo-heme-oxidant or caged radical that initiates arachidonate peroxidation. The reactivity of this oxidant is similar to that of ferryl iron or hydroxyl free radical. Our results suggest that this reaction may be important in myocardial reperfusion injury since reoxygenation of ischemic myocardium results in a burst of xanthine oxidase-generated superoxide and hydrogen peroxide in proximity to cellular myoglobin.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"1 3","pages":"Pages 227-232"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(85)90122-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15053965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 91