DNA (Mary Ann Liebert, Inc.)最新文献_第9页

Complexities for High-Temperature Two-Handed Tile Self-assembly 高温双手瓷砖自组装的复杂性

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-09-24 DOI: 10.1007/978-3-319-66799-7_7

R. Schweller, Andrew Winslow, Tim Wylie

引用次数: 12

The Design Space of Strand Displacement Cascades with Toehold-Size Clamps 带脚尺寸夹具的链位移级联的设计空间

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-09-24 DOI: 10.1007/978-3-319-66799-7_5

Boya Wang, Chris Thachuk, A. Ellington, D. Soloveichik

引用次数: 9

Simplifying Analyses of Chemical Reaction Networks for Approximate Majority 简化近似多数化学反应网络的分析

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-09-24 DOI: 10.1007/978-3-319-66799-7_13

A. Condon, Monir Hajiaghayi, D. Kirkpatrick, Ján Manuch

引用次数: 7

Inferring Parameters for an Elementary Step Model of DNA Structure Kinetics with Locally Context-Dependent Arrhenius Rates 具有局部环境依赖的阿伦尼乌斯速率的DNA结构动力学的基本阶跃模型的参数推断

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-09-24 DOI: 10.1007/978-3-319-66799-7_12

Sedigheh Zolaktaf, Frits Dannenberg, X. Rudelis, A. Condon, Joseph M. Schaeffer, Mark W. Schmidt, Chris Thachuk, E. Winfree

引用次数: 10

Automated, Constraint-Based Analysis of Tethered DNA Nanostructures 捆绑DNA纳米结构的自动约束分析

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-09-24 DOI: 10.1007/978-3-319-66799-7_1

Matthew R. Lakin, Andrew Phillips

引用次数: 2

Chemical Boltzmann Machines 化学玻尔兹曼机

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-07-19 DOI: 10.1007/978-3-319-66799-7_14

W. Poole, Andrés Ortiz-Muñoz, A. Behera, N. S. Jones, T. Ouldridge, E. Winfree, Manoj Gopalkrishnan

引用次数: 28

Robust Detection in Leak-Prone Population Protocols 易泄漏群体协议中的鲁棒检测

DNA (Mary Ann Liebert, Inc.) Pub Date : 2017-06-29 DOI: 10.1007/978-3-319-66799-7_11

Dan Alistarh, Bartłomiej Dudek, A. Kosowski, D. Soloveichik, P. Uznański

引用次数: 20

Mapping contacts between unpurified human progesterone receptor and the hormone response element of mouse mammary tumor virus. 未纯化的人孕酮受体与小鼠乳腺肿瘤病毒激素应答元件的定位联系。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.703

B Kühnel, D el-Ashry, D P Edwards, S K Nordeen

{"title":"Mapping contacts between unpurified human progesterone receptor and the hormone response element of mouse mammary tumor virus.","authors":"B Kühnel, D el-Ashry, D P Edwards, S K Nordeen","doi":"10.1089/dna.1989.8.703","DOIUrl":"https://doi.org/10.1089/dna.1989.8.703","url":null,"abstract":"Binding of steroid hormone receptors to specific recognition sites of hormone-inducible genes is one of the events required for hormonal regulation of gene transcription. We have employed an immunoprecipitation assay to map the interaction between unpurified human progesterone receptors from crude nuclear extracts of T47D cells and the hormone response element of the mouse mammary tumor virus (MMTV). DNase I footprints and methylation interference patterns are similar to those reported with highly purified rabbit progesterone receptors, suggesting that both human and rabbit receptors recognize similar features in the hormone response element. More importantly, these patterns suggest that if other factors are associated with unpurified nuclear receptor, they do not alter the contacts made by receptor nor do they make contacts themselves with MMTV DNA in a manner detected by DNase I or methylation interference assays. The sites of interaction of receptors bound with the clinically important progestin antagonist, RU 486, are comparable to those observed with an agonist-receptor complex. These results suggest that the antagonist prevents receptor action at a step after its recognition and binding to specific sites on a hormone-responsive enhancer element.","PeriodicalId":77708,"journal":{"name":"DNA (Mary Ann Liebert, Inc.)","volume":"8 10","pages":"703-13"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/dna.1989.8.703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13702814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysis of possible repressor elements in the 5'-flanking region of the human beta-globin gene. 人-珠蛋白基因5'侧区可能的抑制元件分析。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.715

J M Krakowsky, E S Panke, R F Lee, J McNeish, S S Potter, J B Lingrel

{"title":"Analysis of possible repressor elements in the 5'-flanking region of the human beta-globin gene.","authors":"J M Krakowsky, E S Panke, R F Lee, J McNeish, S S Potter, J B Lingrel","doi":"10.1089/dna.1989.8.715","DOIUrl":"https://doi.org/10.1089/dna.1989.8.715","url":null,"abstract":"Human beta-globin gene expression is confined predominantly to the adult with little or no expression of this gene occurring during embryonic or fetal life. The lack of expression of this gene in embryonic and fetal erythroid tissue could be due to the absence of required positive regulatory factors in these cells or the presence of negative regulatory factors which prevent expression of the adult globin gene. To test the repressor model, we have used a gel electrophoretic mobility shift assay to identify regions in the human beta-globin gene which bind proteins found in K562 cells, a cell line that expresses embryonic and fetal globins but not adult beta-globin. DNA fragments comprising the entire human beta-globin gene were assayed using nuclear proteins from K562 cells, and four regions were found that bind proteins. These are located within the 5'-flanking region, within the first and second introns, and at the 3'-flanking region of the gene. Previous studies have suggested the presence of potential repressor sites 5' of exon 2. For this reason, we examined whether the lack of the binding regions in the 5'-flanking sequence allow expression of the human beta-globin gene in transgenic mice during embryonic life. beta-globin gene expression was confined to adult life, indicating that if a transcriptional repressor is responsible for inactivating this gene in embryonic tissue, it is not regulated solely by sequences upstream from -122 bp in the 5'-flanking region of the human beta-globin gene.","PeriodicalId":77708,"journal":{"name":"DNA (Mary Ann Liebert, Inc.)","volume":"8 10","pages":"715-21"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/dna.1989.8.715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13754161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Superpolylinkers in cloning and expression vectors. 克隆和表达载体中的超多连接子。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.759

J Brosius

{"title":"Superpolylinkers in cloning and expression vectors.","authors":"J Brosius","doi":"10.1089/dna.1989.8.759","DOIUrl":"https://doi.org/10.1089/dna.1989.8.759","url":null,"abstract":"Versatile DNA polylinkers of more than 300 bp were constructed. They contain the recognition sequences of all restriction enzymes--whether known or still to be discovered--that recognize palindromic hexamers. In addition to these 64 uninterrupted hexameric recognition sites, a number of sites containing interrupted palindromes and nonpalindromic sequences and two recognition sequences with 8 bp are present. Polylinkers (in several variants) were inserted into frequently utilized Escherichia coli cloning vectors such as pBluescript (yielding pSLJ10, pSL250, pSL260, pSL270, and pSL300), pUC18/pUC19 (yielding pSL180 and pSL190, respectively), or pUC118/pUC119 (yielding pSL1180 and pSL1190, respectively). A subtle color discrimination between presence and absence of insert in pSL300 (mid-blue to light-blue or white) was seen in a number of test ligations. The mid-blue color that is generated by pSL300 is presumably due to translational restarts. A different intergenic region for translational restarts was used in plasmids pSL251, pSL261, pSL271, and pSL301. The polylinker was also inserted into expression vector pUC120, yielding pSE1200, and into expression vector pKK233-2, yielding pSE220 and a shortened version thereof, pSE280. Finally, the polylinker was inserted into pTrc99A, resulting in pSE380, which carries a lac repressor gene. This expands the use of the expression system beyond lacIq strains to other bacterial hosts. These versatile vectors have broad applications in genetic engineering.","PeriodicalId":77708,"journal":{"name":"DNA (Mary Ann Liebert, Inc.)","volume":"8 10","pages":"759-77"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/dna.1989.8.759","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13702815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 155