DNA (Mary Ann Liebert, Inc.)最新文献_第10页

A vector that expresses secreted proteins on the cell surface. 在细胞表面表达分泌蛋白的载体。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.753

X B Wang, R Milne, Y Marcel, E Rassart

引用次数: 2

A rapid procedure for cloning genes from lambda libraries by complementation of E. coli defective mutants: application to the fabE region of the E. coli chromosome. 利用大肠杆菌缺陷突变体的互补从λ文库中快速克隆基因的方法:应用于大肠杆菌染色体fabE区。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.779

J H Alix

引用次数: 22

Differential regulation of oncogenic and cellular p185 by serine/threonine kinases. 丝氨酸/苏氨酸激酶对肿瘤和细胞p185的差异调节。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.723

K Dobashi, D B Weiner, M I Greene

引用次数: 16

Structure of the human RD gene: a highly conserved gene in the class III region of the major histocompatibility complex. 人类RD基因的结构:一个高度保守的基因，位于主要组织相容性复合体的III类区域。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.745

P W Speiser, P C White

引用次数: 22

Ultrasonic degradation of DNA. 超声波降解DNA。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.697

H I Elsner, E B Lindblad

{"title":"Ultrasonic degradation of DNA.","authors":"H I Elsner, E B Lindblad","doi":"10.1089/dna.1989.8.697","DOIUrl":"https://doi.org/10.1089/dna.1989.8.697","url":null,"abstract":"Different results are obtained when DNA in aqueous solution and DNA in biological tissue are exposed to ultrasound. At intensities of ultrasound comparable to those applied clinically, ultrasonication is able to degrade purified DNA in aqueous solution, making ultrasonication a useful tool for preparing DNA fragments in vitro. Ultrasonic degradation of DNA in solution occurs by breaking hydrogen bonds and by single-strand and double-strand ruptures of the DNA helix. Two mechanisms are mainly responsible: cavitation and a thermal or mechanical effect. Stable cavitation is seen at low intensities of ultrasound. Increasing the intensity of the ultrasound above 2 W/cm2 is followed by increases in single-strand ruptures due to the creation of free radicals by transient cavitation. Following sonication, the distribution of the resulting DNA fragments approaches a lower size limit of 100-500 bp. Breaks in the DNA helix occur mainly between oxygen and carbon atoms, resulting in DNA fragments with a phosphorylated 5' end and a free alcohol at the 3' end. The relative lack of specificity in degrading the DNA helix makes ultrasonication a complementary alternative to the highly specific fragmentation obtained by restriction endonucleases.","PeriodicalId":77708,"journal":{"name":"DNA (Mary Ann Liebert, Inc.)","volume":"8 10","pages":"697-701"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/dna.1989.8.697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13833380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 102

The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. 人肝型磷酸果糖激酶的初级结构及其与其他类型PFK的比较。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-12-01 DOI: 10.1089/dna.1989.8.733

D Levanon, E Danciger, N Dafni, Y Bernstein, A Elson, W Moens, M Brandeis, Y Groner

{"title":"The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK.","authors":"D Levanon, E Danciger, N Dafni, Y Bernstein, A Elson, W Moens, M Brandeis, Y Groner","doi":"10.1089/dna.1989.8.733","DOIUrl":"https://doi.org/10.1089/dna.1989.8.733","url":null,"abstract":"The complete mRNA sequence of the human liver-type phosphofructokinase (hPFKL) was determined. The sequence included 55 nucleotides of 5' and 515 of 3' noncoding regions, as well as 2,337 nucleotides encoding the 779 amino acids of the hPFKL. Extensive similarity (approximately 90%) in the coding region was observed between the hPFKL and the mouse PFKL, whereas the degree of similarity between different types of PFK, i.e., hPFKL and human muscle-type PFK (hPFKM), was merely 68%. Nevertheless, striking similarity between these different types of PFK was noticed when the amino acid residues creating the various active sites of the enzyme were compared. Human PFK L- and M-specific probes were constructed and used to quantitate the mRNA levels in fetal and adult brains and fetal liver. It was found that while relative amount of PFKL mRNA in adult brain was one-fourth of that detected in fetal brain the level of PFKM mRNA in adult brain was slightly higher than in fetal tissue, suggesting that PFK expression might be controlled at the transcriptional level.","PeriodicalId":77708,"journal":{"name":"DNA (Mary Ann Liebert, Inc.)","volume":"8 10","pages":"733-43"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/dna.1989.8.733","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13677136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase. 人钙调蛋白B (Ca2+/钙调素刺激蛋白磷酸酶的Ca2+结合亚基)cDNA克隆的分离和序列分析。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-11-01 DOI: 10.1089/dna.1.1989.8.675

D Guerini, M H Krinks, J M Sikela, W E Hahn, C B Klee

引用次数: 48

Human immunodeficiency virus type 1 rev protein as a negative trans-regulator. 人类免疫缺陷病毒1型rev蛋白作为负反式调节因子。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-11-01 DOI: 10.1089/dna.1.1989.8.669

M R Sadaie, Z N Benaissa, B R Cullen, F Wong-Staal

引用次数: 6

Alkaline phosphatase fusions to the respiratory syncytial virus F protein as an approach to analyze its membrane topology. 碱性磷酸酶与呼吸道合胞病毒F蛋白融合作为分析其膜拓扑结构的方法。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-11-01 DOI: 10.1089/dna.1.1989.8.659

A Martin-Gallardo, R A Deich, K A Fien, B J Metcalf, A Anilionis, P R Paradiso

{"title":"Alkaline phosphatase fusions to the respiratory syncytial virus F protein as an approach to analyze its membrane topology.","authors":"A Martin-Gallardo, R A Deich, K A Fien, B J Metcalf, A Anilionis, P R Paradiso","doi":"10.1089/dna.1.1989.8.659","DOIUrl":"https://doi.org/10.1089/dna.1.1989.8.659","url":null,"abstract":"Manoil and Beckwith (1985) have constructed a transposon, TnphoA, that permits the generation of hybrid proteins composed of alkaline phosphatase (AP) lacking its signal peptide fused to amino-terminal sequences of other proteins. This transposon has been used to localize export signals and analyze membrane topology of bacterial proteins. We have applied this approach to the membrane fusion protein (F) of respiratory syncytial virus (RSV). The transposon TnphoA and a plasmid directing bacterial expression of the F gene were used to construct F-AP hybrids. These hybrids yielded AP activity, indicating the presence of viral sequences that promoted protein transport through the cytoplasmic membrane. Sequence analysis showed that TnphoA was inserted at four different positions within the F1 subunit. Deletion of the hydrophobic F1 amino-terminus (fusion-related domain) resulted in AP transport to the periplasm, suggesting that the hydrophobic amino-terminus of the F2 subunit is sufficient to promote protein export. Some hybrids were apparently cleaved at or near the F2/F1 junction. The periplasmic localization of an uncleaved hybrid strongly suggested that the fusion-related domain of the F protein, when in the uncleaved F0 precursor, can be moved across the bacterial cytoplasmic membrane. Although these results apply to the recombinant F protein, they agree with the presumed signal sequence and membrane topology of the native F glycoprotein. Thus, this method may be useful in determining membrane topology and in localizing important domains of viral proteins.","PeriodicalId":77708,"journal":{"name":"DNA (Mary Ann Liebert, Inc.)","volume":"8 9","pages":"659-67"},"PeriodicalIF":0.0,"publicationDate":"1989-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/dna.1.1989.8.659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13702816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Determination of exon-intron structure: a novel application of the polymerase chain reaction technique. 外显子-内含子结构的测定:聚合酶链反应技术的新应用。

DNA (Mary Ann Liebert, Inc.) Pub Date : 1989-11-01 DOI: 10.1089/dna.1.1989.8.691

C J Bruzdzinski, T D Gelehrter

引用次数: 6