Diagnostic immunology最新文献_第8页

Influence of racial background on the distribution of T-cell subsets and Leu 11-positive lymphocytes in healthy blood donors. 种族背景对健康献血者t细胞亚群和Leu 11阳性淋巴细胞分布的影响

Diagnostic immunology Pub Date : 1985-01-01

H E Prince, K Hirji, L S Waldbeser, S Plaeger-Marshall, S Kleinman, L L Lanier

{"title":"Influence of racial background on the distribution of T-cell subsets and Leu 11-positive lymphocytes in healthy blood donors.","authors":"H E Prince, K Hirji, L S Waldbeser, S Plaeger-Marshall, S Kleinman, L L Lanier","doi":"","DOIUrl":"","url":null,"abstract":"As part of a study to define the factors affecting the distribution of lymphocyte subpopulations in healthy blood donors, we have measured lymphocyte surface markers in various racial groups. Markers tested were T3 (all T cells), T4A (T helper cells), T8 (T suppressor cells), and Leu 11 (natural killer cells). Racial groups included three Asian groups (Chinese, Japanese, Other Orientals) and three non-Asian groups (Caucasians, Hispanics, American Blacks). The mean percentage of T3 + cells and T4A + cells were significantly lower in Asians compared with non-Asians, while T8 levels did not differ. These changes resulted in a significantly lower mean T4A:T8 ratio in Asians compared with non-Asians. The mean percentage of Leu 11 + cells was higher in Asians compared with non-Asians. Within the Asian group, Chinese had a higher mean Leu 11 + value than the other two Asian groups combined. Further, pairwise comparisons showed that Chinese had a significantly higher mean Leu 11 + value compared with each of the other five racial groups. This increased mean Leu 11 + level in the Chinese group reflected a distinct cluster of high values for about half the subjects. These findings show that racial background should be a major consideration when defining the normal range for lymphocyte subpopulations detected by surface markers.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"3 1","pages":"33-7"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14288227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunotopography of splenic lymphoma of small cleaved B-cell type. 小裂b细胞型脾淋巴瘤的免疫形貌。

Diagnostic immunology Pub Date : 1985-01-01

T M Grogan, C S Rangel, D P Wirt, L C Richter, F N Bever, C S Jolley, H V Villar, S E Jones

{"title":"Immunotopography of splenic lymphoma of small cleaved B-cell type.","authors":"T M Grogan, C S Rangel, D P Wirt, L C Richter, F N Bever, C S Jolley, H V Villar, S E Jones","doi":"","DOIUrl":"","url":null,"abstract":"Using a battery of monoclonal antibodies on snap-frozen sections, we delineated the immunoarchitecture of two splenic small cleaved cell lymphomas (SCL). Both cases had light- and heavy-chain restricted immunoglobulin (lg) expression signifying replacement of splenic white pulp by a single B-cell clone. Both the monotypia and aberrant topography of lg expression in SCL contrasted with the usual polyclonal, zonal lg expression in reactive splenic B-cell zones. Pan B antigens (B1, B4, and L14) were constant in expression as expected for B-cell neoplasms, while B-cell maturation antigens (B2, IgD, and CALLA) were variably expressed, suggesting that different SCL may derive from separate phases of B-cell ontogeny. Close association of SCL with dendritic reticulum cells suggests SCL may derive from splenic secondary follicles or home to these sites. The variable T-cell component detected by a T-cell panel (Leu 1-9) indicates the substantial range of T-cell reactivity in splenic SCL. We emphasize the immunologic aberrancy of splenic SCL when compared to normal splenic B-cell immunotopography. Further, we illustrate the utility of serial tissue section immunochemistry in revealing complex neoplastic cell phenotypes and in revealing the relationships of reactive cells to neoplastic cells.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"3 3","pages":"126-32"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14992289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of human pancreatic adenocarcinomas by histochemical staining with monoclonal antibody AR1-28. 单克隆抗体AR1-28组织化学染色检测人胰腺腺癌。

Diagnostic immunology Pub Date : 1985-01-01

J Chin, F Miller, B P Lane

{"title":"Detection of human pancreatic adenocarcinomas by histochemical staining with monoclonal antibody AR1-28.","authors":"J Chin, F Miller, B P Lane","doi":"","DOIUrl":"","url":null,"abstract":"Hybridoma AR1-28 [Chin and Miller, Cancer Res 45:1723-1729, 1985] was produced using splenocytes from BALB/c mice which had been immunized with human pancreatic adenocarcinoma cell line RWP-1 [Dexter et al, Cancer Res 42:2705-2714, 1982]. This antibody cross reacted with the RWP-2 cell line. AR1-28, an IgG1 antibody, stained the membranes of five (RWP-1, RWP-2, BxPC-3, HPAF-2, and T3M4) out of ten human pancreatic tumor cell lines by immunofluorescence techniques. Electron microscopy on RWP-2 cells, stained by indirect immunoperoxidase, confirmed a membrane location for the AR1-28 antigen. Twenty-three of the 27 clinical specimens (83%) of formalin-fixed, paraffin-embedded pancreatic cancers tested were positive. Varying intensities of staining were observed and were related to the degree of differentiation achieved by the tumor: poorly differentiated tumors showing the least staining while well-differentiated tumors showed the greatest intensity of staining in a predominantly apical location. Immunoblotting showed that AR1-28 reacts with a 200,000-dalton antigen present in extracts of RWP-1 and RWP-2 cells. This monoclonal antibody may be useful in the classification of pancreatic tumor cells.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"3 2","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15014429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MHC class II antigen expression on the various cells of normal and activated isolated pancreatic islets. MHCⅱ类抗原在正常和活化离体胰岛不同细胞上的表达。

Diagnostic immunology Pub Date : 1985-01-01

K Ulrichs, W Müller-Ruchholtz

引用次数: 0

Spontaneous rosette-forming lymphocyte sensitivity to prostaglandins in patients with cervical carcinoma after surgery. 宫颈癌术后自发性玫瑰花形成淋巴细胞对前列腺素的敏感性。

Diagnostic immunology Pub Date : 1985-01-01

D Venza Teti, A Misefari, V Sofo, M F La Via

引用次数: 0

Lymphocyte phenotype changes in congenital and acquired perinatal cytomegalovirus infections. 先天性和获得性围产期巨细胞病毒感染的淋巴细胞表型改变。

Diagnostic immunology Pub Date : 1985-01-01

L D Katikaneni, M F La Via, N M Burdash

{"title":"Lymphocyte phenotype changes in congenital and acquired perinatal cytomegalovirus infections.","authors":"L D Katikaneni, M F La Via, N M Burdash","doi":"","DOIUrl":"","url":null,"abstract":"The recognition that the multiple functions of T-lymphocytes are accomplished by separate subpopulations of cells that can be delineated both functionally and by antisera directed against specific cell surface markers is one major advance in immunology. Our study was directed at enumerating lymphocyte subsets in cytomegalovirus (CMV) perinatal infections with monoclonal antibodies and cell flow cytometry. Eight patients ranging in age from 1 week to 2 1/2 months were studied. Except for one term neonate, all of the infants were less than 36 weeks of gestational age, with a mean of 31.5 weeks (range 27-36 weeks) at birth. The mean birth weight of the premature infants was 1,211 gm (range 870-2420 gm). Five patients were 3 weeks old when studied and were classified as congenital CMV by viral isolation. Three were older than 2 months and were classified as acquired CMV. One of the acquired CMV infants was followed from birth to 6 months of age and had normal lymphocyte numbers at 2 weeks with alterations appearing at 8 weeks. The results of these studies indicated that in congenital CMV there is a normal to high helper/suppressor ratio. On the contrary, in acquired CMV this ratio is profoundly depressed because of significant increase in suppressor T-lymphocytes with borderline normal helper T-lymphocytes. In the latter group, the ratio increased with time after the acute infection episode. Our results agree with reported data indicating that in adults with CMV there is a depression of the helper/suppressor ratio during the acute stage with a return toward normal values during convalescence.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"3 1","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14120372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defect of OKT4 antigen epitopes on normal human lymphocytes found in Japan. 日本正常人淋巴细胞OKT4抗原表位缺陷。

Diagnostic immunology Pub Date : 1985-01-01

K Okubo, Y Sei, K Matsumoto, M M Yokoyama, K Okubo

引用次数: 0

Antigen-specific immunostimulation of amino acid transport: proposed method for the diagnosis of tuberculosis in cattle. 氨基酸运输的抗原特异性免疫刺激:牛结核诊断的建议方法。

Diagnostic immunology Pub Date : 1985-01-01

E H Tate, L S Cram

引用次数: 0

The chemiluminescence response of normal human leukocytes to chlamydia trachomatis. 正常人白细胞对沙眼衣原体的化学发光反应。

Diagnostic immunology Pub Date : 1985-01-01

P Robinson, D Wakefield, D M Graham, J F Easter, R Penny

{"title":"The chemiluminescence response of normal human leukocytes to chlamydia trachomatis.","authors":"P Robinson, D Wakefield, D M Graham, J F Easter, R Penny","doi":"","DOIUrl":"","url":null,"abstract":"The aim of the present study was to investigate the phagocytic response of normal human polymorphonuclear leukocytes (PMN) and monocytes (MN) to eight serotypes of C trachomatis (B,C,D,E,F,I,J, and L2) using a chemiluminescence (CL) assay, with luminal and lucigenin as amplifiers. The magnitude of the phagocytic cell CL response was proportional to the phagocyte-to-chlamydiae ratio, with a poor CL response detected at a ratio of 1:125 and progressively larger CL responses up to ratios of 1:50,000. The durations of the CL responses to all chlamydiae serotypes tested were considerably longer than that for zymosan. The PMN demonstrated a relatively greater CL response to all chlamydiae serotypes tested when compared with MN. The PMN and MN CL responses to \"genital serotypes\" (D,E,F,I, and J) (as well as lymphogranuloma venereum serotype L2) were greater than that for \"ocular\" serotypes (B and C). Inactivation of serum complement and specific chlamydial antibody absorption reduced the CL responses of both PMN and MN. This is the first study to characterize the CL responses of normal human PMN and MN cells to C trachomatis, and it indicates the important role of oxygen dependent antimicrobial systems in the phagocytosis of this common human pathogen.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"3 3","pages":"119-25"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15165317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased natural cell-mediated cytotoxicity per effector cell in acquired immunodeficiency syndrome. 获得性免疫缺陷综合征中每个效应细胞的自然细胞介导的细胞毒性降低。

Diagnostic immunology Pub Date : 1985-01-01

G C Baron, N G Klimas, M A Fischl, M A Fletcher

引用次数: 0