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KB cells for antinuclear antibody determination: comparison with HEp-2 cells and the Crithidia luciliae assay. KB细胞的抗核抗体测定:与HEp-2细胞和水仙法的比较。
Diagnostic immunology Pub Date : 1984-01-01
L B Keil, V A DeBari, M A Needle
{"title":"KB cells for antinuclear antibody determination: comparison with HEp-2 cells and the Crithidia luciliae assay.","authors":"L B Keil,&nbsp;V A DeBari,&nbsp;M A Needle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fifty-seven sera from 53 patients were assayed simultaneously on KB and HEp-2 cells and compared with regard to pattern and titer. Additionally, KB cell fluorescent antinuclear antibody (FANA) titer and pattern in 310 sera from 194 patients were compared with regard to the presence of antinative DNA antibodies (anti-nDNA ab) as determined by the Crithidia luciliae assay. Sixty-five percent (37/57) of the sera had the same titer on both KB and HEp-2 cells; the remainder had higher titers using KB cells. Regression analysis yielded a highly significant, unbiased correlation between the substrates. Forty-four percent (25/57) of these sera gave identical patterns on both substrates, another 25 of the 57 sera (44%) gave different patterns on the two substrates and 12% (7/57) could not be compared because they were negative on HEp-2 cells. KB cells detected positive FANA in 30 of 30 (100%) diagnosed cases of systemic lupus erythematosus; HEp-2 cells detected 29/30 (97%). From the standpoint of sensitivity, these data indicate a slight advantage to the use of KB over HEp-2 cells. Seventeen percent (53/310) of the sera were positive for anti-nDNA ab. The highest percentage of these positive sera occurs at reciprocal FANA titers between 320 and 1280. No association was found between KB FANA patterns and a positive Crithidia luciliae assay.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 4","pages":"213-8"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17455460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme immunoassay detection of IgM to galactoxylomannan of Cryptococcus neoformans. 新型隐球菌半乳糖甘露聚糖IgM的酶免疫检测。
Diagnostic immunology Pub Date : 1984-01-01
E Reiss, R Cherniak, R Eby, L Kaufman
{"title":"Enzyme immunoassay detection of IgM to galactoxylomannan of Cryptococcus neoformans.","authors":"E Reiss,&nbsp;R Cherniak,&nbsp;R Eby,&nbsp;L Kaufman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibodies against the major capsular polysaccharide of Cryptococcus neoformans, glucuronoxylomannan (GXM), and a minor secreted polysaccharide, galactoxylomannan (GalXM), were surveyed by indirect enzyme immunoassay (EIA) in patients with cryptococcosis, with other mycoses, and in normal controls. Measurement of IgG levels against GalXM revealed cross reactions in candidiasis patients that were reduced by adsorption with Candida albicans cell walls. Measurement of IgM levels were subject to fewer cross reactions. The combination of adsorption with C albicans cell walls and measurement of IgM detected antibodies in 12 of 55 cryptococcosis patients. An end point equal to or greater than a titer of 1/16 excluded reactions in normals and limited cross reactivity in candidiasis patients to below 7%. This test has potential diagnostic significance in cryptococcosis patients who show no evidence of cryptococcal antigen circulating in the cerebrospinal fluid or serum. Reactions in this IgM assay were not spuriously due to rheumatoid factor. The major capsular GXM was much less serologically active and was subject to cross reactions with agents of bacterial sepsis. The specificity of the GalXM is directed mainly by the mannose and to a lesser extent by galactosyl residues.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 2","pages":"109-15"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17448663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum immunoglobulin elevations in the acquired immunodeficiency syndrome (AIDS): IgG, IgA, IgM, and IgD. 获得性免疫缺陷综合征(艾滋病)血清免疫球蛋白升高:IgG, IgA, IgM和IgD。
Diagnostic immunology Pub Date : 1984-01-01
Q Chess, J Daniels, E North, N T Macris
{"title":"Serum immunoglobulin elevations in the acquired immunodeficiency syndrome (AIDS): IgG, IgA, IgM, and IgD.","authors":"Q Chess,&nbsp;J Daniels,&nbsp;E North,&nbsp;N T Macris","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The exact nature of the immune defect in the acquired immune deficiency syndrome (AIDS) is not known. Most studies have focused on abnormal T-cell functions which occur in AIDS. Although polyclonal elevation of serum immunoglobulins is also consistently reported in AIDS, there have been no statistical studies measuring the isotypes (classes) comprising this hypergammaglobulinemia. Quantitative serum immunoglobulin levels of IgG, IgA, IgM, and IgD in patients with AIDS (n = 33) were compared to healthy homosexual (n = 71) and healthy heterosexual (n = 32) controls. Serum IgD levels are increased tenfold in AIDS compared to healthy heterosexuals, and threefold compared to healthy homosexuals. Serum IgA levels are increased more than twofold that of either control group. In contrast, the elevations of IgG and IgM are relatively small and show much greater overlap compared to controls. We conclude from a statistical analysis of the data that these elevations of IgD and IgA are characteristics of AIDS.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 3","pages":"148-53"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunologic parameters in premature follicular depletion: T and B lymphocytes, T-cell subpopulations, cutaneous reactivity, and serum immunoglobulin concentrations. 早滤泡衰竭的免疫学参数:T淋巴细胞和B淋巴细胞，T细胞亚群，皮肤反应性和血清免疫球蛋白浓度。
Diagnostic immunology Pub Date : 1984-01-01 DOI: 10.1097/00006254-198601000-00015
C. Friedman, J. Neff, M. H. Kim
{"title":"Immunologic parameters in premature follicular depletion: T and B lymphocytes, T-cell subpopulations, cutaneous reactivity, and serum immunoglobulin concentrations.","authors":"C. Friedman, J. Neff, M. H. Kim","doi":"10.1097/00006254-198601000-00015","DOIUrl":"https://doi.org/10.1097/00006254-198601000-00015","url":null,"abstract":"In 13 women with premature follicular depletion (PFD) the concentration of T and B lymphocytes, T-cell subpopulation, and serum immunoglobulins was determined. Intending to study only women where no obvious cause for PFD existed, including autoimmune disease, five women were excluded because of significant titers of microsomal antibodies or antinuclear antibodies. In the remaining eight women a reduction in the percent of OKT4 (helper) lymphocytes (42.8 +/- 66 vs 50.7 +/- 3.7, P less than 0.005), the ratio of OKT4/OKT8 (suppressor/cytotoxic) lymphocytes (0.96 +/- 0.27 vs 1.6 +/- 0.25, P less than 0.001), and serum concentration of IgA (124 +/- 53 vs 210 +/- 81.5 mg/dl, P less than 0.01) were found compared to a control population. The concentration of T and B lymphocytes, IgG, IgM, and the cutaneous reactivity to mumps and candida antigens were similar in the two populations. It is hypothesized that a mild immune deficiency may predispose to PFD.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"39 11 1","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/00006254-198601000-00015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61950509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Immunodiagnostic tests for protozoan and helminthic infections. 原生动物和蠕虫感染的免疫诊断试验。
Diagnostic immunology Pub Date : 1984-01-01
G I Higashi
{"title":"Immunodiagnostic tests for protozoan and helminthic infections.","authors":"G I Higashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunodiagnostic tests for human protozoan and helminthic infections are reviewed. The need for immunodiagnostic tests varies with each infection but is of paramount importance in those infections that cannot be parasitologically diagnosed readily such as toxoplasmosis, pneumocystosis, Chagas' disease, trichinosis, hydatidosis, cysticercosis, and visceral larva migrans. Immunoassays are also needed for those worldwide highly prevalent infections with severe morbidity to be used in seroepidemiology and in the follow-up evaluation of control programs. The most important are malaria, schistosomiasis, onchocerciasis, lymphatic filariasis, and trypanosomiasis. Major advances have been made in the use of enzyme-linked immunosorbent assay (ELISA) as a practical and rapid test for use in endemic countries and in the identification and isolation of diagnostic parasite antigens aided in particular by the use of monoclonal antibodies. Development of immunodiagnostic tests for specific parasite antigens in body fluids for many infections is being actively pursued.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 1","pages":"2-18"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17497484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation and characterization of murine monoclonal antibodies against HLA Class II molecules. 小鼠抗HLAⅱ类分子单克隆抗体的制备和鉴定。
Diagnostic immunology Pub Date : 1984-01-01
F Malavasi, C Milanese, M Fabbi, P Richiardi, F Caligaris-Cappio, E Ferrero, S Roggero, P Dellabona, R Ceppellini
{"title":"Generation and characterization of murine monoclonal antibodies against HLA Class II molecules.","authors":"F Malavasi,&nbsp;C Milanese,&nbsp;M Fabbi,&nbsp;P Richiardi,&nbsp;F Caligaris-Cappio,&nbsp;E Ferrero,&nbsp;S Roggero,&nbsp;P Dellabona,&nbsp;R Ceppellini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>HLA Class II antigens (human la) are coded by Major Histocompatibility Complex and play important biological roles in health and disease. In this report we describe the generation and characterization of nine murine monoclonal antibodies (MoAbs) specific for determinants localized on the human la molecules. The reactivity of these MoAbs inferred from serological analysis along with the data obtained from biochemical characterization of the target structures allowed a classification of these reagents as monomorphic and polymorphic. Two monomorphic MoAbs, identifying different subsets of human la molecules, were studied in detail.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 1","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17273159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Are lymphocyte subset determinations affected by storage conditions? 淋巴细胞亚群测定是否受储存条件的影响?
Diagnostic immunology Pub Date : 1984-01-01
A D Ponzio, S E Self, D H Runey, N M Burdash, M F La Via
{"title":"Are lymphocyte subset determinations affected by storage conditions?","authors":"A D Ponzio,&nbsp;S E Self,&nbsp;D H Runey,&nbsp;N M Burdash,&nbsp;M F La Via","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of overnight storage, either at room temperature or at 4 degrees C, on total T cells, helper cells, suppressor cells, and HLA-DR-positive cells was determined by flow cytometry and fluoresceinated monoclonal antibodies on peripheral whole blood samples. No differences were noted in any of the subsets regardless of storage conditions when samples were analyzed within 24 hr.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 3","pages":"188-90"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17605384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phagocytosis, bactericidal capacity, and PGE2 production of monocytes in systemic lupus erythematosus and rheumatoid arthritis. 系统性红斑狼疮和类风湿性关节炎中单核细胞的吞噬、杀菌能力和PGE2的产生。
Diagnostic immunology Pub Date : 1984-01-01
A Cruchaud, J P Despont, A Roth, J M Dayer
{"title":"Phagocytosis, bactericidal capacity, and PGE2 production of monocytes in systemic lupus erythematosus and rheumatoid arthritis.","authors":"A Cruchaud,&nbsp;J P Despont,&nbsp;A Roth,&nbsp;J M Dayer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peripheral blood monocytes from 10 systemic lupus erythematosus (SLE) and 10 rheumatoid arthritis (RA) patients as well as from 24 controls were studied for such functions as phagocytosis, bactericidal capacity, iodination, and PGE2 production. Phagocytosis of opsonized erythrocytes, exploring only the Fc receptor, was increased in SLE and RA. Killing of Staphylococcus aureus was decreased in both SLE and RA in the presence of AB serum, but not in the presence of autologous serum. Iodination was, on the average, normal in SLE and elevated in RA. Prostaglandin E2 production was decreased in SLE (except with the highest concentration of Con A) and increased in RA. In SLE, functional alterations were more pronounced in clinically active than in inactive disease. These results show that in SLE and RA peripheral blood monocytes have alterations of their functions that are independent of serum factors. It is suggested that these abnormalities may be relevant to the pathogenetic mechanisms and evolution of these diseases.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 4","pages":"203-12"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17653895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The epidemiology of the acquired immunodeficiency syndrome. 获得性免疫缺陷综合征的流行病学。
Diagnostic immunology Pub Date : 1984-01-01
H W Haverkos, D P Drotman
{"title":"The epidemiology of the acquired immunodeficiency syndrome.","authors":"H W Haverkos,&nbsp;D P Drotman","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 2","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17448664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of platelet antibodies by flow cytometric analysis. 流式细胞术检测血小板抗体。
Diagnostic immunology Pub Date : 1984-01-01
J Lazarchick, P V Genco, S A Hall, A D Ponzio, N M Burdash
{"title":"Detection of platelet antibodies by flow cytometric analysis.","authors":"J Lazarchick,&nbsp;P V Genco,&nbsp;S A Hall,&nbsp;A D Ponzio,&nbsp;N M Burdash","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A rapid, sensitive indirect immunofluorescence assay utilizing flow cytometry to detect immune-mediated thrombocytopenia is described. Fluorescein-conjugated F(ab')2 antihuman IgG or IgGAM is reacted with donor platelets after their incubation with test sera and the resulting immune complex is measured using flow cytometric analysis. With this technique excess of Ig on the platelet, expressed as a ratio of fluorescence of test sera/autologous control sera, was noted in 24 of 30 patients (80%) with immune thrombocytopenias caused by auto- or allo-antiplatelet antibodies. Twenty-four sera from nonimmune thrombocytopenic patients were consistently negative. This technique has the advantage of being highly reproducible and avoids the subjective interpretation inherent with manual immunofluorescence assays.</p>","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"2 4","pages":"238-41"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17455461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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