Diagnostic immunology最新文献_第2页

Application of immunochemistry to the diagnosis of human neoplasms in routine histologic sections. 免疫化学在人类肿瘤常规病理切片诊断中的应用。

Diagnostic immunology Pub Date : 1986-01-01

C J Muus, H A Azar

引用次数: 0

Evaluation of the indirect immunofluorescence assay as a confirmatory test for detecting antibodies to the human immunodeficiency virus. 间接免疫荧光法作为检测人类免疫缺陷病毒抗体的确证试验的评价。

Diagnostic immunology Pub Date : 1986-01-01

T M McHugh, D P Stites, C H Casavant, J R Carlson, J Yee, P A McVay, M P Busch, J A Levy

{"title":"Evaluation of the indirect immunofluorescence assay as a confirmatory test for detecting antibodies to the human immunodeficiency virus.","authors":"T M McHugh, D P Stites, C H Casavant, J R Carlson, J Yee, P A McVay, M P Busch, J A Levy","doi":"","DOIUrl":"","url":null,"abstract":"One hundred and eighty-four serum specimens were assayed for antibodies to the human immunodeficiency virus. All specimens were screened with a commercial enzyme immunoassay and confirmed by two indirect immunofluorescence assays. Sera were also assayed by Western blot. Results from sera of 48 healthy heterosexual volunteers were all negative by EIA, IFA, and Western blot. Sera from 50 healthy homosexual men negative by EIA were also negative by IFA and Western blot. Sixty-two patients with persistent generalized lymphadenopathy or newly diagnosed AIDS all were positive by EIA, IFA, and Western blot. Of 24 sera from patients with autoantibodies, with no evidence of AIDS-related diseases, five appeared to be false-positive by EIA, since they were nonreactive by IFA and Western blot. In addition, three other samples contained both autoantibodies and human immunodeficiency virus antibodies. False-positive results were observed in both the EIA and IFA with monoclonal antibodies directed toward the MHC class II antigens DQ and DR. The reactivity of these antibodies could not be distinguished from positive patients' sera, in either EIA or IFA. We conclude that in general indirect immunofluorescence performed well as a confirmatory test after screening by enzyme immunoassay for human immunodeficiency virus antibodies.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"4 5","pages":"233-40"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14661602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunologic complexity of lymphoblastic lymphoma. 淋巴母细胞淋巴瘤的免疫学复杂性。

Diagnostic immunology Pub Date : 1986-01-01

T Grogan, C Spier, D P Wirt, M J Hicks, M Paquin, J Hutter, T Miller, C Rangel, L Richter, S Jones

{"title":"Immunologic complexity of lymphoblastic lymphoma.","authors":"T Grogan, C Spier, D P Wirt, M J Hicks, M Paquin, J Hutter, T Miller, C Rangel, L Richter, S Jones","doi":"","DOIUrl":"","url":null,"abstract":"Twelve patients with a histologic diagnosis of lymphoblastic lymphoma (LBL) were studied immunologically using the methodologic refinement of comparative serial section immunochemistry. By this means, we demonstrate complex LBL phenotypic profiles, revealing 3 major immunologic subtypes: immature T cell, 7 cases; intermediate or mature T cell, 3 cases; immature B cell (pre-pre-B), 2 cases. This phenotypic diversity challenges the basic belief that all LBL are the same. Our immature T-cell cases with frequent simultaneous Leu 2/3/6/9/CALLA/Tdt expression correspond to cortical thymic phenotypes; our mature T-cell phenotypes with Leu 9/la expression and absent L6/Tdt correspond either to medullary thymocytes or post-thymic T cells; our pre-pre-B phenotypes with simultaneous Tdt/CALLA/B4 expression correspond to common acute lymphocytic leukemia (ALL) phenotypes. Mature T-LBL phenotypes are similar to \"novel\" peripheral T-cell lymphoma phenotypes. Scant or absent Tdt expression in mature LBL is not an isolated antigenic change but a complete phenotypic profile difference from immature T-LBL. The major T- and B-cell phenotypes of LBL might have therapeutic significance. Treatment among LBL phenotypes may need to vary as with acute lymphocytic leukemia phenotypes. Further study is needed; in the meantime, comparative serial section immunotyping promises substantial utility in revealing the immunologic complexity of the lymphomas.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"4 2","pages":"81-8"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14217724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T cells in patients undergoing chronic hemodialysis: mitogenic response, suppressor activity, and interleukin-2 production and receptor generation. 慢性血液透析患者的T细胞:有丝分裂反应，抑制活性，白细胞介素-2的产生和受体的产生。

Diagnostic immunology Pub Date : 1986-01-01

J Raskova, I Ghobrial, S M Shea, E C Ebert, R P Eisinger, K Raska

{"title":"T cells in patients undergoing chronic hemodialysis: mitogenic response, suppressor activity, and interleukin-2 production and receptor generation.","authors":"J Raskova, I Ghobrial, S M Shea, E C Ebert, R P Eisinger, K Raska","doi":"","DOIUrl":"","url":null,"abstract":"The functional response of peripheral blood T lymphocytes was studied in patients with end-stage renal disease treated by chronic hemodialysis for over 1 year. Proliferation after phytohemagglutinin stimulation of patients' peripheral blood mononuclear cells and of T lymphocyte fractions isolated by either sheep erythrocyte rosetting or by use of a nylon wool column was significantly reduced as compared with that of corresponding fractions from healthy control subjects (P less than 0.001). The induction of suppressor cell activity by concanavalin A in rosetted T cell fractions was higher with cells of hemodialyzed patients than with control cells (P less than 0.025). The expression of class II MHC antigen (HLA-DR) by the T8 lymphocyte subset after concanavalin A induction, as determined by staining with monoclonal antibodies and two-color fluorescence analysis by flow cytometry, was also higher in hemodialyzed subjects (P less than 0.025). Since contamination by non-T cells in such cell fractions and increases in proliferation after indomethacin treatment of peripheral blood mononuclear cells were similar in hemodialyzed and control subjects, it is unlikely that the depressed T lymphocyte responses and the increased suppressor cell activity can be attributed to increased peripheral blood monocyte counts observed in patients undergoing hemodialysis. Studies of the biological events associated with the activation of lymphocytes of hemodialyzed patients revealed a reduction in expression of interleukin 2 receptor in the plasma membrane of phytohemagglutinin-stimulated lymphocytes as determined by staining with monoclonal antibody (P less than 0.01). In addition, a very low secretion of interleukin 2 by stimulated peripheral blood mononuclear cell populations was observed in about one-half of patients receiving hemodialysis.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"4 4","pages":"209-16"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14079736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Granulocyte aggregating activity in sera of workers exposed to textile dust inhalation. 纺织粉尘接触工人血清中粒细胞聚集活性的研究。

Diagnostic immunology Pub Date : 1986-01-01

L Varga, M Dervaderics, M Zsiray, J Kárpáthy, G Füst

引用次数: 0

Detection of IgA specific for toxoplasmosis in serum and cerebrospinal fluid using a non-enzymatic IgA-capture assay. 用非酶促IgA捕获法检测血清和脑脊液中弓形虫病的IgA特异性

Diagnostic immunology Pub Date : 1986-01-01

J M Pinon, H Thoannes, P H Pouletty, J Poirriez, J Damiens, P Pelletier

引用次数: 0

Isotypic distribution of human anti-thyroglobulin IgG antibodies: methodological difficulties. 人抗甲状腺球蛋白IgG抗体的同型分布:方法学上的困难。

Diagnostic immunology Pub Date : 1986-01-01

A Barra, P Aucouturier, J L Preud'homme

引用次数: 0

Cell surface phenotype of the spontaneous immunoglobulin-secreting cells in peripheral blood from homosexual men with generalized lymphadenopathy or AIDS. 同性恋男性广泛性淋巴结病或艾滋病患者外周血自发免疫球蛋白分泌细胞的细胞表面表型。

Diagnostic immunology Pub Date : 1986-01-01

L S Martin, J S McDougal, T J Spira, S L Loskoski

引用次数: 0

Early activation marker expression to detect impaired proliferative responses to pokeweed mitogen and tetanus toxoid: studies in patients with AIDS and related disorders. 早期激活标记表达检测对美洲商陆有丝分裂原和破伤风类毒素的增殖反应受损:在艾滋病和相关疾病患者中的研究。

Diagnostic immunology Pub Date : 1986-01-01

H E Prince, J K John

{"title":"Early activation marker expression to detect impaired proliferative responses to pokeweed mitogen and tetanus toxoid: studies in patients with AIDS and related disorders.","authors":"H E Prince, J K John","doi":"","DOIUrl":"","url":null,"abstract":"We tested the premise that measurement of interleukin 2 receptor (IL2R) and transferrin receptor (TR) can be used to assess proliferative responses to pokeweed mitogen (PWM) and tetanus toxoid (TT). Our study group consisted of patients with Human Immunodeficiency Virus (HIV) infection, including patients with acquired immunodeficiency syndrome (AIDS, n = 10), AIDS-related complex (n = 14), lymphadenopathy syndrome (n = 7), or homosexual men seropositive for HIV (n = 6). Controls were 40 healthy seronegative blood donors. IL2R and TR expression by stimulated mononuclear cells were assessed using specific monoclonal antibodies and flow cytometry, and results were analyzed using the 3H-thymidine assay for DNA synthesis as a standard for comparisons. For identifying low PWM responses, both the IL2R+ cell percent and the IL2R+ cell number (no.) per lymphocyte trigger region (a relative measure of IL2R+ cell no. per culture) on day 3 (72 h) were sensitive (greater than 90%) and specific (80%); day 3 TR+ cell no. was also sensitive (92%) and specific (100%). For detecting low TT-induced responses, day 7 IL2R+ cell no. proved the most sensitive (100%) and specific (78%) parameter. These findings indicate that cytofluorometric analysis of IL2R and/or TR expression is a reliable method for detecting impaired proliferative responses to PWM and TT in these patients. Such a method offers an attractive alternative to the regulatory and disposal problems associated with radioactivity in the conventional DNA synthesis assay, as well as providing insight to the mechanism(s) responsible for impaired proliferation.","PeriodicalId":77707,"journal":{"name":"Diagnostic immunology","volume":"4 6","pages":"306-11"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14234253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monoclonal antibodies against isoelectrically focused Nocardia asteroides proteins characterized by the enzyme-linked immunoelectro-transfer blot method. 用酶联免疫电转移印迹法鉴定抗等电聚焦诺卡菌小行星蛋白的单克隆抗体。

Diagnostic immunology Pub Date : 1986-01-01

F A el-Zaatari, E Reiss, M A Yakrus, S L Bragg, L Kaufman

引用次数: 0