Complement (Basel, Switzerland)最新文献_第7页

Biochemistry and pathophysiology of human C1 inhibitor: current issues. 人类C1抑制剂的生物化学和病理生理学:当前的问题。

Complement (Basel, Switzerland) Pub Date : 1985-01-01 DOI: 10.1159/000467851

M Schapira, A de Agostini, J A Schifferli, R W Colman

引用次数: 55

Kinetic study of rheumatoid factor influence on complement-mediated modulation of immune precipitation. 类风湿因子对补体介导的免疫沉淀调节影响的动力学研究。

Complement (Basel, Switzerland) Pub Date : 1985-01-01 DOI: 10.1159/000467863

V D Miletić, B D Rodić

引用次数: 4

Generation of C3d,g and C3d by urokinase-treated plasma in association with fibrinolysis. 尿激酶处理血浆中C3d、g和C3d的产生与纤溶有关。

Complement (Basel, Switzerland) Pub Date : 1985-01-01 DOI: 10.1159/000467857

T Seya, S Nagasawa, M Matsukura, H Hasegawa, J P Atkinson

{"title":"Generation of C3d,g and C3d by urokinase-treated plasma in association with fibrinolysis.","authors":"T Seya, S Nagasawa, M Matsukura, H Hasegawa, J P Atkinson","doi":"10.1159/000467857","DOIUrl":"https://doi.org/10.1159/000467857","url":null,"abstract":"In the breakdown of fluid phase C3 in plasma, the process of conversion of iC3b to C3c and 'C3d' has not been elucidated. Using fluorescent labeled iC3b as a substrate, urokinase (UK) treated but not normal plasma was found to exhibit effective 'C3d' production. To address the relationship between the fibrinolytic activity specific for UK-activated plasma and this 'C3d' production, an assay system was developed that permitted the simultaneous determination of both activities. This method indicated that 'C3d' generation paralleled that of plasmin-dependent fibrinogen degradation. A Km of iC3b for plasmin was 3.0 X 10(-6) mol/l which is similar to the Km of fibrinogen. Plasmin cleavage of iC3b gave rise to C3d1 (Mr 42,000) and C3d2 (Mr 28,000). Purified plasmin C3d1 showed the same Mr and pI as C3d,g prepared from iC3b, by H and I. C3d2 was derived from C3d1. From these in vitro experiments with urokinase-treated plasma, we conclude that in parallel with fibrinolysis efficient cleavage of fluid phase iC3b to C3c + C3d,g and C3d occurs and we hypothesize that this is one mechanism for the generation of C3d in vivo.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"2 2-3","pages":"165-74"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000467857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14070405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Detection of the genetic polymorphism of human C2 (native protein and C2a fragment) by immunoblotting after polyacrylamide gel isoelectric focusing. 聚丙烯酰胺凝胶等电聚焦后免疫印迹法检测人C2(天然蛋白和C2a片段)基因多态性。

Complement (Basel, Switzerland) Pub Date : 1985-01-01 DOI: 10.1159/000467861

B Uring-Lambert, S Gas, J Goetz, G Mauff, S F Goldmann, M Frössler, K Bender, G Hauptmann

{"title":"Detection of the genetic polymorphism of human C2 (native protein and C2a fragment) by immunoblotting after polyacrylamide gel isoelectric focusing.","authors":"B Uring-Lambert, S Gas, J Goetz, G Mauff, S F Goldmann, M Frössler, K Bender, G Hauptmann","doi":"10.1159/000467861","DOIUrl":"https://doi.org/10.1159/000467861","url":null,"abstract":"The polymorphism of the second component of human complement (C2) was studied by means of isoelectric focusing in polyacrylamide gels followed by immunoblotting with a specific antihuman C2 antibody. The polymorphism was studied in native C2 and in the C2a fragment obtained by activation of the classical pathway with heat-aggregated human IgG. Serum samples previously typed with the hemolytic overlay technique were analyzed. They comprised samples of homozygous C2*C, C2*B, C2*Q0, heterozygous C2*BC and C2*CQ0 individuals. The patterns obtained by immunoblotting corresponded to those obtained by the hemolytic overlay technique. As expected, the homozygous C2*Q0 sample (complement C2 deficiency) did not show any band pattern. The C2a fragment presented also a polymorphic variation which correlated exactly with the native C2 polymorphism. It appears thus that the polymorphic site of the C2 protein is carried by the C2a fragment for the C2*C and C2*B variants. In addition, this method is easier to perform than the common hemolytic overlay technique and the rare C2-deficient serum is not needed.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"2 4","pages":"185-92"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000467861","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15027930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Acquired C1 inhibitor deficiency with angioedema symptoms in a patient infected with Echinococcus granulosus. 获得性C1抑制剂缺乏伴细粒棘球绦虫感染的血管性水肿症状

Complement (Basel, Switzerland) Pub Date : 1985-01-01 DOI: 10.1159/000467853

M Cicardi, D Frangi, L Bergamaschini, M Gardinali, G Sacchi, A Agostoni

引用次数: 25

Allotypes of properdin factor B(Bf) and lymphocytotoxic antibody production. 适当因子B(Bf)和淋巴细胞毒性抗体产生的同种异型。

Complement (Basel, Switzerland) Pub Date : 1984-01-01 DOI: 10.1159/000467814

J A Davidson, P A Dyer

引用次数: 1

LPS stimulation of complement (C3) synthesis by a human monocyte cell line. LPS对人单核细胞补体(C3)合成的刺激。

Complement (Basel, Switzerland) Pub Date : 1984-01-01 DOI: 10.1159/000467823

W K Nichols

{"title":"LPS stimulation of complement (C3) synthesis by a human monocyte cell line.","authors":"W K Nichols","doi":"10.1159/000467823","DOIUrl":"https://doi.org/10.1159/000467823","url":null,"abstract":"The human monocyte-like cell line, U937, was utilized as a model system to assess the influence of lipopolysaccharides (LPS) from Escherichia coli and other immunomodulatory agents on the biosynthesis of complement component C3 by monocytoid cells. The amount of C3 accumulated in the medium of cultured cells was measured by an enzyme-linked immunoabsorbent assay (ELISA). In the presence of LPS (0.01 and 0.10 microgram/ml) C3 production by U937 cells was increased by 2- and 5-fold, respectively. C3 production was also increased in the presence of lymphokine-containing supernatants obtained from human peripheral blood mononuclear cells after their stimulation with concanavalin A-sepharose. Human gamma-interferon (50-500 units/ml) stimulated C3 production by U937 cells. Stimulation of C3 production by LPS or mononuclear cell supernatants was blocked by cycloheximide. LPS (0.10 and 1.0 microgram/ml) also stimulated PGE2 production by U937 cells but failed to increase PGE2 at the lowest concentration (0.01 micrograms/ml) shown to increase C3 biosynthesis. Although exogenous PGE1 (10(-7) and 10(-6) M) promoted a small increase in C3 production, the effect of LPS was not decreased by a concentration of indomethacin (10(-6) M) that inhibited biosynthesis of PGE2. Therefore, LPS-induced C3 synthesis is not mediated by an increase in PGE2 in U937 cells. In summary, these observations suggest that C3 biosynthesis by monocytes/macrophages can be modulated by mediators of cellular immune responses found in the microenvironment of a localized inflammatory reaction.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"1 2","pages":"108-15"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000467823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17654737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Distributions and recoveries of complement components during cold ethanol fractionation of human plasma. 人血浆乙醇冷分离过程中补体成分的分布和回收率。

Complement (Basel, Switzerland) Pub Date : 1984-01-01 DOI: 10.1159/000467834

Y Fukumoto, K Tsumoto, A Tashiro, N Ohtomo, T Yoshida

引用次数: 1

Rapid, homogeneous phase, liposome-based assays for total complement activity. 快速，均相，基于脂质体的总补体活性测定。

Complement (Basel, Switzerland) Pub Date : 1984-01-01 DOI: 10.1159/000467828

G Akots, J C Braman, R J Broeze, D W Bowden

引用次数: 9

Prelytic reduction of high-energy phosphates induced by antibody and complement in nucleated cells. 31P-NMR study. 有核细胞中抗体和补体诱导的高能磷酸盐的预裂解还原。31日p-nmr研究。

Complement (Basel, Switzerland) Pub Date : 1984-01-01 DOI: 10.1159/000467839

R Tirosh, H Degani, G Berke

引用次数: 9