Complement (Basel, Switzerland)最新文献_第3页

C3-like activity in C3-deficient dog serum. c3缺陷犬血清中c3样活性。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463007

J P Johnson

{"title":"C3-like activity in C3-deficient dog serum.","authors":"J P Johnson","doi":"10.1159/000463007","DOIUrl":"https://doi.org/10.1159/000463007","url":null,"abstract":"A complete absence of the third component of complement has been demonstrated in a colony of Brittany spaniels. The deficiency is inherited in an autosomal recessive fashion and is clinically characterized by an increased susceptibility to infection and renal disease. Despite having no detectable antigenic C3, C3-deficient dog serum has approximately 10% of normal serum C3 hemolytic activity. This report characterizes the nature of the C3-like activity in C3-deficient dog serum. The activity is inhibited by heating serum to 56 degrees C for 30 min and by incubation in 0.01 M EDTA. The activity is also sensitive to both methylamine and D-76, in a dose-dependent fashion. The lytic activity in C3-deficient dog serum appears to be complement mediated. Furthermore, lysis of EAC1,4,oxy2 cells by deficient dog serum is inhibited by methylamine. Although lysis is boosted by the addition of purified C5, a greater boost is obtained in the absence of methylamine. Therefore, there exists a methylamine-sensitive protein other than C4 in C3-deficient dog serum which interacts with C5. It is possible that this protein may be a newly discovered complement protein for which appropriate conditions for its detection have not previously been available.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 1","pages":"53-60"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14237114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A sensitive specific hemolytic assay for proenzyme C1. 原酶C1的敏感特异溶血试验。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463006

A J Tenner, M M Frank

{"title":"A sensitive specific hemolytic assay for proenzyme C1.","authors":"A J Tenner, M M Frank","doi":"10.1159/000463006","DOIUrl":"https://doi.org/10.1159/000463006","url":null,"abstract":"The traditional hemolytic assay of the functional activity of C1, the first component of the classical complement pathway, was modified to permit differentiation between proenzyme (unactivated) C1 and the activated state of the enzyme (C1). A two-step assay was developed to quantitate proenzyme C1. The C1 sample to be assayed was first preincubated with C1 inhibitor, a process that specifically inhibits the enzymatic activity of C1 without affecting the subsequent activation of proenzyme C1 by EAC4, a model immune complex. Since the rate of reaction between C1 inhibitor, a serum regulatory protein, and C1 is concentration-dependent, this step is performed at high C1 and C1 inhibitor concentrations. Subsequent dilutions of the sample prevents C1 inhibitor-mediated inactivation of the C1 that is activated during the C1 hemolytic assay. Thus, in the presence of C1 inhibitor, the level of C1 hemolytic activity specifically reflects the activity of proenzyme C1, while in the absence of C1 inhibitor, the hemolytic activity reflects the total activity of C1. Both the absolute and the relative amounts of the proenzyme (unactivated) and activated C1 can thereby be quantitated in most samples. Furthermore, a partially purified C1 inhibitor reagent, easily prepared from serum, was shown to function identically to the purified C1 inhibitor, obviating the need for a multistep isolation procedure for this protein. Using this simple yet sensitive assay to investigate the efficiency of reconstitution of C1 activity from the purified components C1q, C1r, and C1s, we also find evidence for temperature- and concentration-dependent reaction steps in the formation of functional C1.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 1","pages":"42-52"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14674579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Studies on the hemolytic activity of the classical and alternative pathway of complement in various animal species. 不同动物补体经典途径和替代途径溶血活性的研究。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463005

S Tanaka, F Kitamura, T Suzuki

引用次数: 17

Effects of lysine and glutamic acid or [corrected] Mg++ on the conformations of C3 and B, and the activation of the alternative complement pathway. 赖氨酸和谷氨酸或[修正]Mg++对C3和B构象的影响，以及替代补体途径的激活。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463014

A Takada, Y Takada, Y Makino, Y Sugawara, S Shirahama

{"title":"Effects of lysine and glutamic acid or [corrected] Mg++ on the conformations of C3 and B, and the activation of the alternative complement pathway.","authors":"A Takada, Y Takada, Y Makino, Y Sugawara, S Shirahama","doi":"10.1159/000463014","DOIUrl":"https://doi.org/10.1159/000463014","url":null,"abstract":"The conversion of C3 and B in the mixture of C3, B, D and Mg++ ions was inhibited in the presence of arginine and lysine, but not in the presence of glutamic acid and aspartic acid among other amino acids. Application of dialyzed plasma to a lysine-Sepharose column resulted in elution of B and a part of D in pass-through fractions, and C3 and the other part of D were retained in the column, subsequently eluted by increase in salt concentrations. C3, B and a part of D were eluted in pass-through fractions, and C3 and the other part of D were retained in the column, subsequently eluted by increase in salt concentrations. C3, B and a part of D were eluted in pass-through fractions when applied to glutamic acid-Sepharose. A highly purified D preparation appeared in the pass-through fraction of the lysine-Sepharose column, suggesting that D may form a complex in plasma. The intensity of intrinsic fluorescence of C3 decreased in the presence of arginine to the largest extent. Lysine affected the intensity less than arginine. Kd was calculated to be 0.42 mM for arginine and 0.55 mM for lysine in the interaction with C3. The intensity of intrinsic fluorescence of B decreased in the presence of aspartic acid and glutamic acid (Kd = 0.48 mM for aspartic acid and 0.24 mM for glutamic acid). Arginine or lysine affected the intensity of B less than those anionic amino acids. The presence of Mg++ ions resulted in a decrease in the fluorescence intensity of C3 and B.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 2","pages":"110-9"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14024550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of a spontaneously shed fragment of B cell complement receptor type two (CR2) containing the C3d-binding site. 含有c3d结合位点的B细胞补体受体2 (CR2)自发脱落片段的鉴定。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463012

B L Myones, G D Ross

{"title":"Identification of a spontaneously shed fragment of B cell complement receptor type two (CR2) containing the C3d-binding site.","authors":"B L Myones, G D Ross","doi":"10.1159/000463012","DOIUrl":"https://doi.org/10.1159/000463012","url":null,"abstract":"CR2 is a 140-kilodalton glycoprotein expressed on B lymphocytes which binds to both C3d and Epstein-Barr virus (EBV). The present study identified a 72-kilodalton C3d-binding protein (gp72) in the spent culture media of Raji B lymphoblastoid cells as a spontaneously shed fragment of the 140-kilodalton CR2 molecule. Both polyclonal and monoclonal antibodies (AB) were used in several assay systems to detect antigenic determinants shared between the gp72 fragment and CR2. Rabbit antibodies to either intact CR2 or gp72 blocked C3d receptor activity, and this inhibitory activity was removed by absorption of anti-CR2 with purified gp72.OKB7, a monoclonal anti-CR2 AB that blocks both C3d and EBV binding to CR2, reacted specifically with both CR2 and gp72, whereas both anti-B2 and HB-5 monoclonal anti-CR2 AB, that block neither of these receptor sites, were unreactive with gp72. The data suggested that the gp72 fragment was not present as such in intact cells, but rather was a product of cells generated by proteolysis of CR2. Thus, intrinsically labeled gp72 was isolated from Raji cell media by affinity chromatography on OKB7-Sepharose, but only intact CR2 was isolated from the Raji cell fraction solubilized in the presence of protease inhibitors. Several lines of evidence suggested that gp72 was not a second type of C3d receptor that was distinct from CR2. First, Raji cells expressed nearly identical amounts of OKB7 and HB-5 epitopes when analyzed by flow cytometry or radioimmune assay, excluding the possibility that B cells expressed OKB7 antigens in both CR2 and a distinct HB-5-negative C3d receptor. Second, all Raji cell surface C3d receptor activity was associated with HB-5-reactive CR2 molecules. We conclude that gp72 represents a spontaneously shed proteolytic fragment of CR2 that contains the C3d-binding site and the closely associated OKB7 epitope.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 2","pages":"87-98"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14620290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Specificity of membrane complement receptor type three (CR3) for beta-glucans. 膜补体受体3型(CR3)对β -葡聚糖的特异性。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463010

G D Ross, J A Cain, B L Myones, S L Newman, P J Lachmann

{"title":"Specificity of membrane complement receptor type three (CR3) for beta-glucans.","authors":"G D Ross, J A Cain, B L Myones, S L Newman, P J Lachmann","doi":"10.1159/000463010","DOIUrl":"https://doi.org/10.1159/000463010","url":null,"abstract":"The binding of the iC3b receptor (CR3) to unopsonized zymosan was shown to result from CR3 attachment to cell wall beta-glucans. A specificity of neutrophil responses for beta-glucan was first suggested by a comparison of yeast (Saccharomyces cerevisiae) cell wall components for stimulation of a neutrophil superoxide burst. Neutrophils responded poorly to heat-killed yeast, but gave increasingly better responses to cell wall polysaccharides devoid of proteins (zymosan) and nearly pure beta-glucan particles derived from zymosan. Zymosan triggered a burst that was 29% as great as that stimulated by phorbol myristate acetate (PMA), and beta-glucan particles stimulated a burst that was 72% as great as that produced by PMA. Phagocytic responses to yeast were also inhibited by soluble glucans but not by soluble mannans. Three types of experiments demonstrated a role for CR3 in these responses. First, neutrophil ingestion of either yeast or yeast-derived beta-glucan particles was blocked by monoclonal anti-CR3, fluid-phase iC3b, or soluble beta-glucan from barley. Monocyte ingestion of beta-glucan particles was also blocked by anti-CR3, but not by anti-CR1 or anti-C3. Second, the neutrophil superoxide burst response to either zymosan or beta-glucan particles was blocked by anti-CR3 or fluid-phase iC3b, and was completely absent with neutrophils from 3 patients with an inherited deficiency of CR3. Third, CR3 was isolated from solubilized neutrophils by affinity chromatography on beta-glucan-Sepharose.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 2","pages":"61-74"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14172379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 222

Purification and characterisation of the fourth component of bovine complement. 牛补体第四组分的纯化与鉴定。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463002

D M Groth, J D Wetherall, B A Umotong, P Sparrow, I R Lee, M J Carrick

{"title":"Purification and characterisation of the fourth component of bovine complement.","authors":"D M Groth, J D Wetherall, B A Umotong, P Sparrow, I R Lee, M J Carrick","doi":"10.1159/000463002","DOIUrl":"https://doi.org/10.1159/000463002","url":null,"abstract":"A relatively rapid method for the isolation of complement protein C4 from bovine plasma is described. The method consists of DEAE Sephacel anion exchange chromatography of plasminogen-depleted bovine plasma followed by cation exchange chromatography on CM Sepharose and finally gel filtration on a TSK G3000 SW column. A yield of approximately 20% was obtained. Conventional SDS-PAGE of purified bovine C4 showed the presence of alpha, beta and gamma polypeptide chains, the molecular weights of which were determined from Ferguson plots to be 95,000 +/- 2,500, 80,500 +/- 2,000 and 30,000 +/- 500 daltons, respectively. SDS-PAGE of C4 immunoprecipitated from the plasma of individual cattle in gels with a reduced proportion of crosslinker showed size polymorphism of the alpha chain. The presence of dual alpha chains was confirmed by radiolabelling their reactive thiol ester moiety with 14C methylamine. The difference in size of the two bovine alpha chains is approximately 1,800 daltons. On activation of bovine C4 both alpha chains were cleaved into alpha' chains (87,000 and 85,000 daltons) characteristic of C4b.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14690557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

XIIth International Complement Workshop. September 18-21, 1987, Chamonix, France. Abstracts. 第十二届国际补品研讨会。1987年9月18日至21日，法国夏蒙尼。摘要。

Complement (Basel, Switzerland) Pub Date : 1987-01-01

引用次数: 0

Analysis of C3 and C4 in ovine plasma. 绵羊血浆中C3和C4的分析。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463003

D M Groth, J D Wetherall, P M Outteridge, R G Windon, B Richards, I R Lee

{"title":"Analysis of C3 and C4 in ovine plasma.","authors":"D M Groth, J D Wetherall, P M Outteridge, R G Windon, B Richards, I R Lee","doi":"10.1159/000463003","DOIUrl":"https://doi.org/10.1159/000463003","url":null,"abstract":"The distributions of plasma concentrations of complement proteins C3 and C4 were studied in sample populations of merino and Suffolk sheep. No differences between the breeds or the sexes were observed. The distribution for ovine C4 was polymodal and very disperse relative to that for C3. It was found, however, that C3 concentrations were elevated in specimens from 20 merino sheep bred as high responders to a Trichostrongylus vaccine. Significantly decreased plasma C4 concentrations were observed in representatives of both merino and merino X Border Leicester cross-bred sheep affected with congenital progressive ovine muscular dystrophy. Agarose gel electrophoretic variants of ovine C3 were not detected. Evidence for electrophoretic variants of ovine C4 in agarose gels was found although individual allotypes could not be reliably identified. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) did not reveal size heterogeneity for the alpha and beta chains of immunoprecipitated ovine C3. Analysis of reduced immunoprecipitated ovine C4 by SDS-PAGE revealed considerable size heterogeneity in the alpha chain consistent with isotypic and/or allotypic variability. The data presented strongly suggest the presence of two C4 loci in sheep, each of which exhibits polymorphism.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 1","pages":"12-20"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14690558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Complement activation by human lymphocytes from different lymphoid organs: role of sialic acid and lack of relationship to electrical surface charge. 来自不同淋巴器官的人淋巴细胞的补体活化:唾液酸的作用和缺乏与表面电荷的关系。

Complement (Basel, Switzerland) Pub Date : 1987-01-01 DOI: 10.1159/000463013

C Gutierrez, M J Martin, K A Brown

{"title":"Complement activation by human lymphocytes from different lymphoid organs: role of sialic acid and lack of relationship to electrical surface charge.","authors":"C Gutierrez, M J Martin, K A Brown","doi":"10.1159/000463013","DOIUrl":"https://doi.org/10.1159/000463013","url":null,"abstract":"A study was made of the complement activation (CA) capacity of human lymphocytes from lymphoid organs. Whereas thymocytes did not show complement membrane fluorescence (CMF) following incubation in normal homologous serum (NHS), a mean of 18 and 34%, respectively, of tonsil and spleen cells were positive for antihuman C3 fluorescent serum. Isolated T cells did not show CA capacity, while 62% of a purified tonsil B population possessed this capacity. The CMF was abolished by adding EDTA and when the incubation was performed in C1q and factor-D-depleted serum. Addition of EGTA, supplemented with MgCl2 to NHS failed to abolish the fluorescence, indicating that the alternative pathway was involved in the phenomenon. Removal of sialic acid with neuraminidase increased the percentage of cells showing CMF. Since sialic acid significantly contributes to the extent of net negative surface charge, we have studied whether this parameter influences the CA capacity. Tonsil B cells were separated in fractions with different electrophoretic mobility by preparative cell electrophoresis, and similar percentages of CMF-positive cells were found in all fractions. Therefore, it is likely that the capacity to activate complement is unrelated to the membrane electrical charge.","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"4 2","pages":"99-109"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000463013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14621574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4