Collagen and related research最新文献_第5页

Pseudoxanthoma Elasticum (PXE): Ultrastructural and Biochemical Study on Proteoglycan and Proteoglycan-Associated Material Produced by Skin Fibroblasts In Vitro 皮肤成纤维细胞体外产生蛋白聚糖及蛋白聚糖相关物质的超微结构和生化研究

Collagen and related research Pub Date : 1988-01-01 DOI: 10.1016/S0174-173X(88)80035-9

R. Tiozzo^costa , M. Baccarani Contri , M.R. Cingi , I. Pasquali^ronchetti , R. Salvini , S. Rindi , G. De Luca

{"title":"Pseudoxanthoma Elasticum (PXE): Ultrastructural and Biochemical Study on Proteoglycan and Proteoglycan-Associated Material Produced by Skin Fibroblasts In Vitro","authors":"R. Tiozzo^costa , M. Baccarani Contri , M.R. Cingi , I. Pasquali^ronchetti , R. Salvini , S. Rindi , G. De Luca","doi":"10.1016/S0174-173X(88)80035-9","DOIUrl":"10.1016/S0174-173X(88)80035-9","url":null,"abstract":"<div><p>Pseudoxanthoma elasticum is a genetic disease characterized by progressive mineralization of elastic fibers. Previous studies suggested that other components, apart from elastin, might be involved in the alterations of this connective tissue disorder (Martinez-Hernandez and Huf f er, 1974; Pasquali Ronchetti et al., 1981; 1986). Evidence is presented that proteoglycan metabolism is altered in PXE-affected patient. Urinary GAGs suggests an increased degradation of glucosamine-containing GAGs in the patient. Pulse and chase experiments on <em>in vitro</em> skin fibroblasts indicated a decreased rate of synthesis of <sup>35</sup>SO<sub>4</sub> containing GAGs or an increase of their turnover rate in PXE. Moreover, when PGs produced from skin fibroblasts were identified by ultracentrifugation and gel filtration in associative conditions, PXE fibroblasts produced a significantly higher amount of the high molecular weight fraction of sulfated PGs. This high molecular weight material was present both in the medium and in the matrix and disappeared under dissociative conditions or after treatment with hyaluronidase or with pancreas elastase. By electron microscopy, PXE fibroblasts appeared to produce and secrete an enormous amount of toluidine blue 0 positive material organized as filaments and amorphous masses. These data are in agreement with previous observations of the presence of abnormal masses of microfilaments, in the dermis of PXE patients, which were sensitive to hyaluronidase and partially to trypsin and elastase (Pasquali Ronchetti et al., 1986). The results seem to confirm that at least some of the alterations of connective tissues in PXE are due to abnormal PGs metabolism and to their tendency to form abnormal aggregates in the extracellular space.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"8 1","pages":"Pages 49-64"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(88)80035-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14470517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Monoclonal Antibodies Against Synovial Collagenase: Use for Immunopurification and Characterization of the Latent and Active Enzyme 抗滑膜胶原酶单克隆抗体:用于免疫纯化和鉴定潜伏酶和活性酶

Collagen and related research Pub Date : 1988-01-01 DOI: 10.1016/S0174-173X(88)80031-1

A. Crespo , M. Duchesne , T. Cartwright , C. Pernelle , J.M. Cherel

引用次数: 6

Quantification and Characterization of Hyaluronic Acid inDifferent Topographical Areas of Normal Articular Cartilage from Dogs 狗正常关节软骨不同地形区域透明质酸的定量和表征

Collagen and related research Pub Date : 1988-01-01 DOI: 10.1016/S0174-173X(88)80034-7

Daniel H. Manicourt , Julio C. Pita

引用次数: 16

Streptozotocin-induced Diabetes and the Rat Periodontium: Decreased Relative Collagen Production 链脲佐菌素诱导的糖尿病与大鼠牙周组织:相对胶原蛋白生成减少

Collagen and related research Pub Date : 1988-01-01 DOI: 10.1016/S0174-173X(88)80042-6

Michael Schneir , Michael Imberman , Nangavaram Ramamurthy , Lorne Golub

引用次数: 19

Authors Index 作者索引

Collagen and related research Pub Date : 1987-12-01 DOI: 10.1016/S0174-173X(87)80043-2

引用次数: 0

Noncollagenous Proteins of Basement Membranes 基底膜的非胶原蛋白

Collagen and related research Pub Date : 1987-12-01 DOI: 10.1016/S0174-173X(87)80042-0

Mats Paulsson

引用次数: 41

Eriochrome Black T Inhibition of Human Skin Collagenase, but not Gelatinase, Using Both Protein and Synthetic Substrates 蛋白质和合成底物对人皮肤胶原酶而非明胶酶的抑制作用

Collagen and related research Pub Date : 1987-12-01 DOI: 10.1016/S0174-173X(87)80038-9

J.L. Seltzer , M.L. Eschbach , J.O. Winberg , E.A. Bauer , A.Z. Eisen , H. Weingarten

{"title":"Eriochrome Black T Inhibition of Human Skin Collagenase, but not Gelatinase, Using Both Protein and Synthetic Substrates","authors":"J.L. Seltzer , M.L. Eschbach , J.O. Winberg , E.A. Bauer , A.Z. Eisen , H. Weingarten","doi":"10.1016/S0174-173X(87)80038-9","DOIUrl":"10.1016/S0174-173X(87)80038-9","url":null,"abstract":"<div><p>The intracellular degradation of interstitial collagen is accomplished by two neutral metalloproteases, collagenase and gelatinase. Both enzymes are inhibited by metal chelating agents, by certain sulfhydryl reagents, and by similar protein inhibitors. Here, we demonstrate that the dye eriochrome black T (EBT) appears to be unique in its capacity to inhibit collagenase but not gelatinase. Using native reconstituted helical collagen in gel form at 37°C, half-maximal inhibiton of collagenase activity by EBT occurs at ~ 45 [tM. EBT more effectively inhibits the breakdown of native collagen in solution, with a K<sub>I</sub> of ~ 8 ¼M. Using a newly-developed spectrophotometric substrate, AcProLeuGly-S-LeuLeuGly-OC<sub>2</sub>H<sub>5</sub>, a K<sub>I</sub> of 1.4 ¼M was calculated for EBT on collagenase. Although this same thiopeptolide serves as a substrate for gelatinase with kinetics similar to those of collagenase, no inhibition by EBT was observed. EBT also did not inhibit the gelatinase-mediated breakdown of the natural substrate, gelatin. The data suggest that EBT may have significant potential for allowing the differentiation in biological fluids of two metalloproteases with similar cleavage site specificities.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"7 6","pages":"Pages 399-407"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(87)80038-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13970753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

In Vivo Administration of Antibodies Against Type I Collagen in Rat: The Specific Accumulation in Spleen 抗I型胶原抗体在大鼠体内给药:脾脏特异性积聚

Collagen and related research Pub Date : 1987-12-01 DOI: 10.1016/S0174-173X(87)80037-7

Gregory L. Idelson, Vladimir R. Muzykantov , Elena E. Chekneva, Alexander A. Shnyra, Boris V. Shekhonin, Sergey P. Domogatsky

{"title":"In Vivo Administration of Antibodies Against Type I Collagen in Rat: The Specific Accumulation in Spleen","authors":"Gregory L. Idelson, Vladimir R. Muzykantov , Elena E. Chekneva, Alexander A. Shnyra, Boris V. Shekhonin, Sergey P. Domogatsky","doi":"10.1016/S0174-173X(87)80037-7","DOIUrl":"10.1016/S0174-173X(87)80037-7","url":null,"abstract":"<div><p>[<sup>125</sup>1]-labelled rabbit antibodies against rat type I collagen and non-immune IgG were injected into rat circulation. The kinetics of their clearance and the biodistribution in different organs were studied. Both preparations showed very similar clearance rate, the kinetics fitting bi-exponential approximation with characteristic parameters t<sub>1,1/2</sub> = 201 ± 20 min before 320 min and t<sub>1,1/2</sub> = 1350 ± 450 min at times over 320 min for antibodies and 258 ± 45 min and 890 ± 140 min for IgG. The specific affinity of the circulating antibodies did not decrease within 24 hours. The antibodies were specifically accumulated in spleen, where their accumulation was 5-fold higher than that of nonimmune IgG. Accumulation of antibodies was maximal 3 hours after the injection. The localization ratio (i.e. the ratio of the amount of the antibodies per g of tissue to that per g of blood) reached a maximum 24 hours after the injection and remained stable for 120 hours. Immunofluorescent staining of spleen sections resulted in a bright fluorescence of dense collagenous structures in the trabeculae and in the wall of the central follicular arterium, bright spot fluorescence in the marginal zone of the follicle, and diffuse fluorescence in the red pulp. These findings suggest an unusually high accessibility of collagen type I in spleen to circulating blood plasma components.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"7 6","pages":"Pages 383-397"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(87)80037-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14454265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The Normal Variation in Structure and Composition of Canine Hip Articular Cartilage Proteoglycans 犬髋关节关节软骨蛋白聚糖结构和组成的正常变化

Collagen and related research Pub Date : 1987-12-01 DOI: 10.1016/S0174-173X(87)80040-7

Sven Inerot , Dick Heinegård

引用次数: 4

Collagen and Non-Collagenous Protein Content (Osteocalcin, Sialoprotein, Proteoglycan) in the Iliac Crest Bone and Serum Osteocalcin in Women with and without Hand Osteoarthritis 髂嵴骨中的胶原蛋白和非胶原蛋白(骨钙素、唾液蛋白、蛋白多糖)含量以及患有和不患有手骨关节炎的女性血清骨钙素

Collagen and related research Pub Date : 1987-12-01 DOI: 10.1016/S0174-173X(87)80041-9

Greet Gevers, Jan Dequeker

{"title":"Collagen and Non-Collagenous Protein Content (Osteocalcin, Sialoprotein, Proteoglycan) in the Iliac Crest Bone and Serum Osteocalcin in Women with and without Hand Osteoarthritis","authors":"Greet Gevers, Jan Dequeker","doi":"10.1016/S0174-173X(87)80041-9","DOIUrl":"10.1016/S0174-173X(87)80041-9","url":null,"abstract":"<div><p>Quantitative and qualitative variations in bone composition were studied in postmortem iliac crest bone specimens from 32 women aged 60 to 75, grouped according to the presence of osteoarthritis at the hand joints. The EDTA extraction yield of macromolecules was significantly greater in the osteoarthritis group. Collagen and osteocalcin content were significantly increased and proteoglycan content decreased in the osteoarthritis group. No difference in sialoprotein content was found.</p><p>In a clinical study of 20 patients with generalised osteoarthritis, we found compared to 15 controls also significantly increased serum levels of osteocalcin.</p><p>These results indicate in an unselected group of cases that differences in bone matrix constituents exist between elderly women with and without an osteoarthritic constitution. The finding that these alterations are present even far away from the articular lesion, and the fact that the serum osteocalcin levels are elevated, indicate that osteoarthritis is part of a more generalised bone disease and not a purely cartilage disease.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"7 6","pages":"Pages 435-442"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(87)80041-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14259833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47