Collagen and related research最新文献

Increased Collagenase Activity is not Detectable in Cervical Softening in the Ewe 在母羊的颈椎软化中没有检测到胶原酶活性的增加

Collagen and related research Pub Date : 1988-11-01 DOI: 10.1016/S0174-173X(88)80010-4

John G. Raynes , John C. Anderson , R.J. Fitzpatrick , Hilary Dobson

{"title":"Increased Collagenase Activity is not Detectable in Cervical Softening in the Ewe","authors":"John G. Raynes , John C. Anderson , R.J. Fitzpatrick , Hilary Dobson","doi":"10.1016/S0174-173X(88)80010-4","DOIUrl":"10.1016/S0174-173X(88)80010-4","url":null,"abstract":"<div><p>Cervical tissue from ewes at various stages of pregnancy was examined for evidence that collagenase is involved in the process of cervical softening. Collagenase activity was detected in medium after 2–3 days culture of ovine cervical explants, but there was no significant difference in total enzymic activity produced by explants from non-pregnant, early pregnant or late pregnant animals when expressed as units/mg wet weight of tissue over five days in culture. Oestradiol infusion into ewes prior to parturition did not alter the enzyme activity subsequently produced in explant culture. However, the DNA concentration, and hence the number of cells per unit volume, decreased significantly with length of pregnancy, this effect being due to expansion of cervical tissue which occurs late in pregnancy. Thus, if collagenase activity is expressed relative to DNA and hence cell number, there is evidence for increased production per cell in order to keep the tissue concentration constant. However, as the concentration of collagen in cervix remains constant during pregnancy, the ratio of collagenase activity to collagen is also constant. It is therefore concluded that there is no evidence of a role for increased collagenase activity in cervical softening in the ewe.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"8 6","pages":"Pages 461-469"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(88)80010-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13987767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Abstracts From The Second International Conference On Molecular Biology And Pathology Of Matrix' Philadelphia, Pennsylvania, June 15-18,1988 1988年6月15日至18日在美国宾夕法尼亚州费城举行的第二届国际基质分子生物学和病理学会议摘要

Collagen and related research Pub Date : 1988-11-01 DOI: 10.1016/S0174-173X(88)80011-6

Darwin J. Prockop (a)

引用次数: 0

Authors Index 作者索引

Collagen and related research Pub Date : 1988-11-01 DOI: 10.1016/S0174-173X(88)80012-8

引用次数: 0

International Epidermolysis Bullosa Symposium, Regent's College, London, U.K. 国际大疱性表皮松解症研讨会，摄政学院，英国伦敦

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80020-7

引用次数: 0

A Hard Look in Collagen Related Discorders Symposium, Liegè, Belgium 胶原蛋白相关疾病研讨会，Liegè，比利时

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80021-9

引用次数: 0

Quantitation of Collagen Fragments and Gelatin by Deconvolution of Polarimetry Denaturation Curves 用极化法变性曲线反褶积法定量胶原碎片和明胶

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80014-1

Richard A. Condell , Naomi Sakai , Ruth T. Mercado , Ed Larenas

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引用次数: 10

Synthesis of Type II Collagen is Decreased in Cartilage Cultured with Interleukin 1 while the Rate of Intracellular Degradation Remains Unchanged 在白细胞介素1培养的软骨中，II型胶原的合成减少，而细胞内降解的速度保持不变

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80013-X

Jenny A. Tyler , Hilary P. Benton

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引用次数: 50

Bone Induction in Intramuscular Implants by Demineralized Bone Matrix: Sequential Changes of Collagen Synthesis 脱矿骨基质诱导肌内植入物成骨:胶原合成的顺序变化

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80015-3

Irvin A. Guterman , Thomas E. Boman , Gwo-Jaw Wang , Gary Balian

{"title":"Bone Induction in Intramuscular Implants by Demineralized Bone Matrix: Sequential Changes of Collagen Synthesis","authors":"Irvin A. Guterman , Thomas E. Boman , Gwo-Jaw Wang , Gary Balian","doi":"10.1016/S0174-173X(88)80015-3","DOIUrl":"10.1016/S0174-173X(88)80015-3","url":null,"abstract":"<div><p>Implantation of rat demineralized bone matrix into intramuscular pouches has beenshown to cause a complex cellular transition of mesenchymal-type cells into well developed mature bone. Demineralized bone matrix was surgically implanted into rat muscle pouches and removed at various intervals between 7 and 28 days. Histological sections of the implants revealed bone formation by endochondral ossification and appositional bone growth. Biochemical analysis of collagen synthesis demonstrated the following: (1) synthesis of type X collagen, a collagen produced by hypertrophic chondrocytes in the growth plate and in fracture callus. (2) Synthesis of a collagenase-sensitive 17k protein which seems to increase in the early stages of bone induction. Pulse chase analysis indicates that 17k is not a degradation product of another protein and appears to be synthesized without a large M<sub>r</sub> precursor. The 17k component contains one or more collagenous domains that are partially resistant to proteolysis with pepsin. Our results confirm the appearance of a cartilage intermediate during demineralized bone matrix induced ossification and implicate the existence of proteins which may be useful markers in future studies on matrix mineralization and ossification.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"8 5","pages":"Pages 419-431"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(88)80015-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14355233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Dose-dependence of and Threshold for Optimal Bone Induction by Collagenous Bone Matrix and Osteogenin-enriched Fraction 胶原骨基质和富骨原素组分诱导最佳骨的剂量依赖性和阈值

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80016-5

N. Muthukumaran , S. Ma , A.H. Reddi

引用次数: 68

The Drosophila Homoeotic Gene Spalt is Structurally Related to Collagen αl(IV) Chain 果蝇同源基因Spalt与胶原α 1 (IV)链结构相关

Collagen and related research Pub Date : 1988-09-01 DOI: 10.1016/S0174-173X(88)80018-9

Panagiotis A. Tsonis , Paul F. Goetinck

引用次数: 0