Arteriosclerosis (Dallas, Tex.)最新文献_第2页

Myosin isoform expression and smooth muscle cell heterogeneity in normal and atherosclerotic rabbit aorta. 正常和动脉粥样硬化兔主动脉肌球蛋白异构体表达和平滑肌细胞异质性。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.996

A M Zanellato, A C Borrione, M Tonello, G Scannapieco, P Pauletto, S Sartore

{"title":"Myosin isoform expression and smooth muscle cell heterogeneity in normal and atherosclerotic rabbit aorta.","authors":"A M Zanellato, A C Borrione, M Tonello, G Scannapieco, P Pauletto, S Sartore","doi":"10.1161/01.atv.10.6.996","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.996","url":null,"abstract":"Two monoclonal antimyosin antibodies, Western blotting experiments, and immunofluorescence procedures were used to investigate myosin isoform expression in normal and atherosclerotic aortas of adult rabbits. The SM-E7 antibody reacted with the two myosin heavy chain (MHC) isoforms of smooth muscle (SM) type (SM-MHC-1 and SM-MHC-2) expressed in the adult rabbit aorta. The NM-G2 antibody recognized an epitope shared by the nonmuscle (NM) myosin heavy chains (NM-MHC) present in fibroblasts, macrophages, lymphocytes, and platelets. Two smooth muscle cell (SMC) populations were identified in the medial layer of normal adult aorta, namely cells that contained SM myosin exclusively and cells that showed the coexistence of SM and NM myosin isoforms. The size of the cell population with double myosin isoform content increased markedly during experimental atherogenesis and represented by far the predominant SMC phenotype in the atherosclerotic plaque. Western blotting analysis performed on crude extracts from the atherosclerotic plaque showed the presence of SM-MHC-1 and NM-MHC isoforms in this tissue. Co-expression of SM and NM myosin at the molecular and the cellular level were found in aortic tissue during the early stages of development. These results indicate that in experimental atherosclerosis, the accumulation in the plaque of SMC with an \"immature\" pattern of myosin isoform expression is accompanied by similar modifications in the differentiation pattern of SMC of the underlying media.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"996-1009"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12865024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 94

Regulation of hepatic high density lipoprotein binding proteins after administration of simvastatin and cholestyramine to rats. 辛伐他汀和胆胺对大鼠肝脏高密度脂蛋白结合蛋白的调节作用。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1045

D Mathai, N Fidge, M Tozuka, A Mitchell

{"title":"Regulation of hepatic high density lipoprotein binding proteins after administration of simvastatin and cholestyramine to rats.","authors":"D Mathai, N Fidge, M Tozuka, A Mitchell","doi":"10.1161/01.atv.10.6.1045","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.1045","url":null,"abstract":"We investigated the regulation of putative high density lipoprotein (HDL) receptors in rat liver after cholesterol feeding and the administration of cholesterol-lowering drugs to rats. The expression of two plasma membrane HDL binding proteins (HB1 and HB2) were compared in control and treated livers by first separating membrane proteins on sodium dodecyl sulfate-polyacrylamide gels and quantitating HB1 and HB2 levels with a specific ligand blot assay. Of the various treatments used, only simvastatin or simvastatin plus cholestyramine produced significant changes, with reductions of up to 40% and 60%, respectively, for HB1 and HB2. The effect on the binding proteins was not associated with changes in serum cholesterol concentrations, which did not change significantly after either treatment, although a marked rise in liver cholesterol concentration after cholesterol was associated with a moderate increase in HB2 expression. We show evidence for regulation of the levels of hepatic HDL binding proteins and provide another important criterion for the acceptance of HB1 and HB2 as components of a functional HDL receptor.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"1045-50"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.1045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13326822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Role of elastase and lysyl oxidase activity in spontaneous rupture of internal elastic lamina in rats. 弹性酶和赖氨酸氧化酶活性在大鼠内弹性板自发破裂中的作用。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1136

M J Osborne-Pellegrin, J Farjanel, W Hornebeck

{"title":"Role of elastase and lysyl oxidase activity in spontaneous rupture of internal elastic lamina in rats.","authors":"M J Osborne-Pellegrin, J Farjanel, W Hornebeck","doi":"10.1161/01.atv.10.6.1136","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.1136","url":null,"abstract":"Rupture of the internal elastic lamina may occur spontaneously with age in certain arteries of the rat and to various extents in different strains. This phenomenon may have some bearing on certain aspects of arterial pathology. For this study, we investigated biochemically the mechanisms of formation of interruptions in the internal elastic lamina (IIEL) by comparing aortas of Brown Norway (BN) rats, which develop numerous IIEL in the abdominal aorta, with those of Long-Evans (LE) rats, which develop none. We isolated aortic elastin from BN and LE rats and determined its amino acid composition and its susceptibility to different elastases. No differences were found between the two strains, but the quantity of elastin isolated per aorta was lower in the BN than in the LE rats. Elastase-like activity (ELA) of whole aortic extracts, measured with Suc(Ala)3NA as a substrate, was greater in the BN rats than in the LE rats of both sexes. The assay of ELA in endothelium, media, and adventitia extracted separately showed very low levels in the media compared to the endothelium and adventitia. The endothelium accounts for about one-half of the total aortic ELA, but a difference between the two strains was detected only in the adventitia. With 3H-insoluble elastins prepared from BN and LE aortas as substrates, elastinolytic activity (EA) was detected only in extracts of endothelium after prior exposure to trypsin. Extracts from BN endothelium on BN elastin were more active than were those from LE endothelium on LE elastin. The assay of lysyl oxidase activity in aortic extracts from the two strains with 3H-collagen from chick embryo calvaria as the substrate showed a lower activity in the BN than in the LE rats. Taken together, these results suggest that increased elastase activity and decreased lysyl oxidase activity may be involved in the formation of IIEL.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"1136-46"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.1136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13138021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Relationship of endogenous sex steroid hormones to lipids and apoproteins in postmenopausal women. 绝经后妇女内源性性类固醇激素与脂质和载脂蛋白的关系。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1058

L H Kuller, J P Gutai, E Meilahn, K A Matthews, P Plantinga

{"title":"Relationship of endogenous sex steroid hormones to lipids and apoproteins in postmenopausal women.","authors":"L H Kuller, J P Gutai, E Meilahn, K A Matthews, P Plantinga","doi":"10.1161/01.atv.10.6.1058","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.1058","url":null,"abstract":"The relationships between blood levels of estrogen and lipoprotein lipids and apoproteins were evaluated in 120 women early in the climacteric. Among women who were 1-year amenorrheic, not taking hormone replacement therapy, and with follicle-stimulating hormone levels greater than 720 ng/ml, serum estradiol levels were positively related to concentrations of the high density lipoprotein 2 cholesterol (HDL2c) subfraction. There was a substantial decrease in HDL2c and apoprotein (apo) A-I in women whose estradiol levels decreased to less than or equal to 2.5 pg/ml from the first to the second postmenopausal examination. In a sample of women evaluated during the perimenopause (3-months' amenorrheic), those with the highest concentrations of estradiol or estrone showed a (nonsignificantly) higher level of HDL2c and a lower level of low density lipoprotein cholesterol (LDLc) than did those with the lowest concentration of estradiol or estrone. Estradiol levels declined dramatically between the perimenopausal and the postmenopausal examinations, and this was accompanied by a decrease in HDL2c and a nonsignificant increase in LDLc. HDL2c levels fell substantially in those women whose estradiol decreased below the sensitivity of the assay. The change, however, was not statistically significant. Estrone is the primary postmenopausal estrogen, and levels are directly related to obesity, as are levels of insulin. The interrelationship among obesity, conversion of estrone to estradiol at the tissue level, and insulin (or insulin sensitivity) is probably the primary determinant of HDLc concentration among postmenopausal women.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"1058-66"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.1058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13278227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of coronary artery atherosclerosis by 17-beta estradiol in ovariectomized monkeys. Lack of an effect of added progesterone. 17- β雌二醇对去卵巢猴子冠状动脉粥样硬化的抑制作用。缺乏添加黄体酮的效果。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1051

M R Adams, J R Kaplan, S B Manuck, D R Koritnik, J S Parks, M S Wolfe, T B Clarkson

{"title":"Inhibition of coronary artery atherosclerosis by 17-beta estradiol in ovariectomized monkeys. Lack of an effect of added progesterone.","authors":"M R Adams, J R Kaplan, S B Manuck, D R Koritnik, J S Parks, M S Wolfe, T B Clarkson","doi":"10.1161/01.atv.10.6.1051","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.1051","url":null,"abstract":"Although controversy continues, the preponderance of evidence indicates that estrogen replacement therapy favorably influences the risk of coronary heart disease in postmenopausal women. It remains uncertain how this effect is mediated and whether the cyclic addition of a progestin may influence adversely an estrogen-related cardioprotective effect. We investigated the influence of sex hormone replacement therapy on diet-induced coronary artery atherosclerosis in estrogen-deficient (ovariectomized) adult female cynomolgus monkeys. Monkeys were assigned randomly to one of three treatment groups: 1) no hormone replacement (n = 17), 2) continuously administered 17-beta estradiol plus cyclically administered progesterone (n = 20), and 3) continuously administered 17-beta estradiol (n = 18). The physiologic patterns of plasma estradiol and progesterone concentrations were maintained by administering the hormones in sustained-release subcutaneous Silastic implants. The experiment lasted 30 months. At necropsy, coronary artery atherosclerosis was inhibited similarly (reduced by approximately one-half) in animals in both hormone replacement groups (p less than or equal to 0.05). Antiatherogenic effects of hormone replacement were independent of variation in total plasma cholesterol, lipoprotein cholesterol, apoprotein A-1 and B concentrations, high density lipoprotein subfraction heterogeneity, and low density lipoprotein molecular weight. We conclude that physiologic estrogen replacement therapy with or without added progesterone inhibits atherosclerosis progression in ovariectomized monkeys. This may explain why estrogen replacement therapy results in reduced risk of coronary heart disease in postmenopausal women.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"1051-7"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.1051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13396601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 625

Arteriosclerosis: the first 10 years. 动脉硬化:前10年。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.959

E L Bierman, A G Motulsky, R Ross, J G White

引用次数: 0

Whole blood impedance platelet aggregometry and ischemic heart disease. The Caerphilly Collaborative Heart Disease Study. 全血阻抗血小板聚集与缺血性心脏病。卡菲利合作心脏病研究。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1032

P C Elwood, A D Beswick, D S Sharp, J W Yarnell, S Rogers, S Renaud

{"title":"Whole blood impedance platelet aggregometry and ischemic heart disease. The Caerphilly Collaborative Heart Disease Study.","authors":"P C Elwood, A D Beswick, D S Sharp, J W Yarnell, S Rogers, S Renaud","doi":"10.1161/01.atv.10.6.1032","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.1032","url":null,"abstract":"The Caerphilly Collaborative Heart Disease Study is based on a large cohort of men who were ages 49 to 64 years at the time of the study. We report the results for platelet aggregation measured in whole blood from a subsample of 308 men. The index of sensitivity used was the minimum concentration of adenosine diphosphate that produced a defined degree of impedance change in the Chronolog 560 aggregometer. There was a marked association between aggregation and prevalent ischemic heart disease (IHD). The odds ratios and 95% confidence intervals (CI) for prevalent IHD in men with the most sensitive platelets compared with those with the least sensitive platelets were 3.6 (95% Cl: 1.1 to 12.2) for angina; 7.3 (95% Cl: 2.0 to 24.3) for previous myocardial infarction (MI); and 2.7 (95% Cl: 1.0 to 7.6) for electrocardiogram evidence of ischemia. The confidence limits for these odds ratios are large because of the small sample size, but the estimates of odds ratio are relatively large compared to similar relationships between the traditional risk factors of serum cholesterol, blood pressure, smoking, and prevalent IHD (1.5 to 2.5). A number of factors that might confound the relationships between platelets and IHD were examined, but the associations remained statistically significant when these were taken into account.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"1032-6"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.1032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13396619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 68

Vascular responses to endothelin-1 in atherosclerotic primates. 动脉粥样硬化灵长类血管对内皮素-1的反应。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1113

J A Lopez, M L Armstrong, D J Piegors, D D Heistad

引用次数: 54

Abnormal composition of hypertriglyceridemic very low density lipoprotein determines abnormal cell metabolism. 异常组成的高甘油三酯血症极低密度脂蛋白决定异常细胞代谢。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1088

E Sehayek, S Eisenberg

{"title":"Abnormal composition of hypertriglyceridemic very low density lipoprotein determines abnormal cell metabolism.","authors":"E Sehayek, S Eisenberg","doi":"10.1161/01.atv.10.6.1088","DOIUrl":"https://doi.org/10.1161/01.atv.10.6.1088","url":null,"abstract":"The metabolism of very low density lipoprotein (VLDL) from normolipemic (NTG) subjects, hypertriglyceridemic (HTG) subjects, and hypertriglyceridemic subjects treated with bezafibrate (BZ) was studied in cultured human skin fibroblasts. The binding, cell association, and proteolytic degradation of 125I-labeled lipoproteins and the capacity to regulate cellular sterol synthesis was determined with and without maximal stimulation of the lipoprotein by exogenous recombinant or plasmatic apolipoprotein (apo) E-3. The VLDL was separated into three density subfractions: I, II, and III. Multiple differences between HTG and NTG lipoproteins were found, which all reverted toward normal with therapy. Even in the presence of an optimal concentration of apo E-3, HTG-VLDL demonstrated 100% to 200% higher metabolic activities, indicating a better association or a better biological expression of apo E-3 at the surface of the lipoprotein. There was a strong and linear relationship between the cholesterol ester/protein ratios of the different VLDLs and their proteolytic degradations by the cells (r = 0.95). Thus, the composition/structure alterations of VLDL appear to determine their apo E-3-dependent cellular catabolism. In addition, HTG-VLDLs not enriched with apo E-3 exhibited a capacity to down-regulate cellular sterol synthesis independently of their uptake and degradation by the cells. This abnormality appeared to reflect the ability of the VLDL to donate cholesterol to the cells and was not observed in receptor-negative cells. Thus, HTG-VLDL is much more capable than NTG-VLDL of introducing cholesterol to cells by at least two mechanisms: 1) accelerated uptake and degradation and 2) direct transfer of cholesterol to the cells. Both processes are potentially atherogenic and are reversible when triglyceride-lowering therapy is instituted.","PeriodicalId":77492,"journal":{"name":"Arteriosclerosis (Dallas, Tex.)","volume":"10 6","pages":"1088-96"},"PeriodicalIF":0.0,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1161/01.atv.10.6.1088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13396603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Serum lipid profile in an elderly Chinese population. 中国老年人群的血脂分析。

Arteriosclerosis (Dallas, Tex.) Pub Date : 1990-11-01 DOI: 10.1161/01.atv.10.6.1097

J Woo, C W Lam

引用次数: 19