Progress in growth factor research最新文献_第5页

Matrix metalloproteinases as insulin-like growth factor binding protein-degrading proteinases 基质金属蛋白酶作为胰岛素样生长因子结合蛋白降解蛋白酶

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)00017-8

John L. Fowlkes , Kathryn M. Thrailkill , Delila M. Serra , Ko Suzuki , Hideaki Nagase

{"title":"Matrix metalloproteinases as insulin-like growth factor binding protein-degrading proteinases","authors":"John L. Fowlkes , Kathryn M. Thrailkill , Delila M. Serra , Ko Suzuki , Hideaki Nagase","doi":"10.1016/0955-2235(95)00017-8","DOIUrl":"10.1016/0955-2235(95)00017-8","url":null,"abstract":"<div><p>Insulin-like growth factor (IGF) bioavailability is modulated by specific IGFBPs, six of which are known (IGFBPs 1–6). Since IGFBPs have equal or higher affinity for IGFs than do IGF receptors, it is believed that degradation of IGFBPs by IGFBP-degrading proteinases is an important step in regulating IGF bioactivity. Recent studies from our laboratory have demonstrated that at least two IGFBPs, i.e. IGFBP-3 and -5, are degraded under physiologic conditions by matrix metalloproteinases (MMPs). <em>In vitro</em>, we have demonstrated that IGFBP-3 is degraded in human dermal fibroblast cultures by MMPs using a variety of techniques, including proteinase inhibition with a specific inhibitor of MMPs, i.e. tissue inhibitor of metalloproteinases (TIMP-1), immunoabsorption with specific antisera to human MMPs and a unique method developed in our laboratory, IGFBP-3 substrate zymography. Using similar methods, we have also demonstrated that MMPs, along with an unidentified 97-kDa proteinase, degrade IGFBP-5 in murine osteoblast cultures. In rat pregnancy serum, we have shown that degradation of IGFBP-3 is associated with MMP activity present in the serum, which likely arises from the placental compartment. Analysis of the cleavage products of IGFBP-3 produced by MMPs 1, 2 and 3 reveals that MMPs cleave exclusively within the non-homologous, mid-region of the molecule. Together, these studies suggest that MMPs, beyond their previously described roles as extracellular matrix degrading enzymes, may also exert effects on cellular growth and differentiation via degradation of IGFBPs.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 255-263"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(95)00017-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19785518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 143

Cell migration: Interactions among integrins, IGFs and IGFBPs 细胞迁移:整合素、igf和igfbp之间的相互作用

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)00015-1

John I. Jones, Monica E. Doerr, David R. Clemmons

{"title":"Cell migration: Interactions among integrins, IGFs and IGFBPs","authors":"John I. Jones, Monica E. Doerr, David R. Clemmons","doi":"10.1016/0955-2235(95)00015-1","DOIUrl":"10.1016/0955-2235(95)00015-1","url":null,"abstract":"<div><p>The migratory behaviour of cells is fundamental to diverse biologic processes such as tumor metastasis, development of atherosclerotic plaques, embryonic development and wound healing. We have examined the effects of IGF-I and IGFBPs on the migration of Chinese Hamster ovary (CHO) cells, smooth muscle cells (SMC) and human breast cancer cells (HBC) and have studied the involvement of integrin receptors in migration induced by IGF-I and by IGFBPs.</p><p>Using a monolayer wounding assay, we determined the effect of IGFBP-1 on SMC to be qualitatively similar to its effect we reported earlier on CHO cells, in that there is a direct stimulation of migration mediated by the α5β1 integrin. IGFBP-2 has no direct effect on SMC migration, and although it also contains the ArgGlyAsp sequence, we can detect no integrin binding. Unlike CHO cells, SMC are stimulated to migrate by IGF-I. IGFBP-2 and IGFBP-1 both inhibit this IGF-I receptor-mediated stimulation. We have also studied the migration of HBC using a Boyden chamber apparatus and have shown a potent chemotactic effect of IGF-I.</p><p>We have investigated the mechanisms for IGF-I stimulation of SMC and HBC migration. IGF-I stimulation of SMC migration requires the presence of either 0.2% serum or vitronectin, because of a requirement for ligand binding by the αVβ3 integrin (vitronectin receptor). MCF-7 HBC migrate toward a concentration gradient of IGF-I, the only growth factor that was able to stimulate these cells to migrate. Integrin ligand binding was also necessary for MCF-7 cells to migrate in response to IGF-I; αVβ5 integrin was required for migration on vitronectin and α2β1 was required on collagen.</p><p>These studies demonstrate that the stimulation of cell migration by IGFBP-1 and IGF-I involves signaling by members of the integrin family of receptors. The mechanisms by which the IGF-I receptor and integrin receptors interact are not yet known.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 319-327"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(95)00015-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19785525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 82

Functions and regulation of the acid-labile subunit of the 150 K complex 150k配合物酸不稳定亚基的功能和调控

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)00006-2

A. Barreca, P. Ponzani, A. Arvigo, A. Voci, G. Giordano, F. Minuto

{"title":"Functions and regulation of the acid-labile subunit of the 150 K complex","authors":"A. Barreca, P. Ponzani, A. Arvigo, A. Voci, G. Giordano, F. Minuto","doi":"10.1016/0955-2235(95)00006-2","DOIUrl":"10.1016/0955-2235(95)00006-2","url":null,"abstract":"<div><p>In normal subjects, the major form of circulating IGF is the GH-dependent 150 K complex. As demonstrated by gel-permeation chromatography, the acid-labile subunit (ALS) purified from human serum, incubated for 2 h at 20°C with [<sup>125</sup>I]IGF-I and rIGFBP-3, is able to increase not only the molecular weight (mol. wt.) of the IGF-IGFBP-3 complex, but also the amount of IGF-I bound. In both charcoal and polyethylene glycol ligand binding assays, competitive binding curves for the displacement of [<sup>125</sup>I]IGF-I from rIGFBP-3 by increasing concentrations of unlabeled IGF-I showed an increased binding activity of rIGFBP-3 in the presence of ALS. The effect of ALS on rIGFBP-3 binding activity was dose dependent. In addition, ligand and immunoblot revealed that ALS and rIGFBP-3 are able to form a high mol. wt. complex in the absence of IGF peptide. On the basis of these data, ALS seems to have a more complex function than that of simply increasing the mol. wt of the IGF-IGFBP-3 complex.</p><p>The regulation of ALS synthesis by rat hepatocytes in primary culture has also been evaluated by immunoblot. In agreement with the <em>in vivo</em> finding of an inhibitory effect of octreotide (a somatostatin analog) on the formation of the 150 K complex in acromegalic subjects, we could observed <em>in vitro</em> that octreotide produces a dose-dependent inhibition of ALS secretion into the hepatocyte conditioned medium. TGF-β1 was also inhibitory at high doses. On the contrary, we could not evidence any effect of IGF-I or IGF-II, while a small increase has been noted after incubation with T3.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 231-239"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(95)00006-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19785571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

In vivo actions of IGF analogues with poor affinities for IGFBPs: Metabolic and growth effects in pigs of different ages and GH responsiveness 对igfbp亲和力较差的IGF类似物的体内作用:不同年龄猪的代谢和生长影响以及生长激素反应性

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)00007-0

P.E. Walton , F.R. Dunshea , F.J. Ballard

{"title":"In vivo actions of IGF analogues with poor affinities for IGFBPs: Metabolic and growth effects in pigs of different ages and GH responsiveness","authors":"P.E. Walton , F.R. Dunshea , F.J. Ballard","doi":"10.1016/0955-2235(95)00007-0","DOIUrl":"10.1016/0955-2235(95)00007-0","url":null,"abstract":"<div><p>IGF-I analogues that bind poorly to IGFBPs are substantially more potent than IGF-I at stimulating growth in rats. However, rodents differ from other mammals because they contain only minimal circulating levels of IGF-II and they are poorly responsive to GH. In this report we review a series of experiments carried out in pigs, a species that is both GH responsive and has high blood concentrations of IGF-II. Intravenous bolus administration of IGFs to 55 kg pigs depressed blood glucose with the potency greatest for analogues such as des(1–3)IGF-I, R<sup>3</sup>IGF-I and LongR<sup>3</sup>IGF-I that showed the weakest binding to pig IGFBP-3, a similar efficacy pattern to that reported in the rat. Chronic subcutaneous administration of LongR<sup>3</sup>IGF-I, however, reduced growth rates, led to a depression in food intake and lowered concentrations of IGF-I, IGF-II and IGFBP-3. IGF-I itself depressed IGF-II concentrations and did not stimulate growth. Subcutaneous infusion of IGFs over a 3-day period, also in 55 kg pigs, demonstrated that analogues that bound least well to IGFBP-3 were the most effective at reducing the concentration of this binding protein, suggesting that the inhibition of growth was related to the depression of IGFBP-3. On the other hand, IGF-I and LongR<sup>3</sup>IGF-I increased growth rats in neonatal pigs, especially under conditions of reduced food intake. As these anabolic effects occur at a developmental stage where the animals are insensitive to GH in a manner analogous to the situation in rats, it is plausible that the feed-back inhibition of GH secretion explains the catabolic response to IGFs in older pigs.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 385-395"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(95)00007-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19785989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Regulation of IGFBP-3 expression in breast cancer cells and uterus by estradiol and antiestrogens: Correlations with effects on proliferation: A review 雌二醇和抗雌激素对乳腺癌细胞和子宫中IGFBP-3表达的调节:与增殖影响的相关性:综述

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)00036-4

Huynh Hung, Michael Pollak

引用次数: 12

Acknowedgement Acknowedgement

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)90000-4

引用次数: 0

Modulation of human IGF binding protein-3 activity by structural modification 结构修饰对人IGF结合蛋白-3活性的调节

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(95)00004-6

Robert C. Baxter, Sue M. Firth

{"title":"Modulation of human IGF binding protein-3 activity by structural modification","authors":"Robert C. Baxter, Sue M. Firth","doi":"10.1016/0955-2235(95)00004-6","DOIUrl":"10.1016/0955-2235(95)00004-6","url":null,"abstract":"<div><p>To delinate regions of IGFBP-3 involved in ligand and cell-surface binding, DNAs encoding human IGFBP-3[1–264] and several variants were transfected into CHO cells. Of three deletion (Δ) mutants, IGFBP-3[1–88], [1–184], and [Δ89–184], none bound IGF-I tracer by ligand blotting, although all were detectable by immunoblotting. No ALS binding was detectable, as predicted by the lack of IGF binding. Normal-sequence IGFBP-3 associated with the CHO cells and was partly displaceable by IGF-I. Whereas IGFBP-3[1–88] and [1–184] failed to cell-associate, the non-IGF-binding central deletion variant [Δ89–184] did associate with CHO cells but was not displaced by IGF-I. To further examine the role of the carboxy-terminal domain in cell-association, the basic sequence IGFBP-3[228–232] (KGRKR) was altered to the corresponding IGFBP-1 residues MDGEA, a major charge reversal. This variant showed reduced IGF-I binding, and bound ALS with decreased affinity as determined by Scatchard analysis. It showed no cell binding, implicating the basic domain in cell-association. We conclude that, whereas the central and carboxy-terminal domain deletions fail to bind IGF-I, the ability to cell associate requires the carboxy-terminal but not the central domain. Specifically, the basic region [228–232] is essential for cell binding, and also affects IGF-I binding, and independently, ALS affinity.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 215-222"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(95)00004-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19785569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Conservation of IGFBP structure during evolution: Cloning of chicken insulin-like growth factor binding protein-5 IGFBP在进化过程中的结构保护:鸡胰岛素样生长因子结合蛋白5的克隆

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(96)00011-7

Susanne V. Allander , Ewa Ehrenborg , Holger Luthman , David R. Powell

{"title":"Conservation of IGFBP structure during evolution: Cloning of chicken insulin-like growth factor binding protein-5","authors":"Susanne V. Allander , Ewa Ehrenborg , Holger Luthman , David R. Powell","doi":"10.1016/0955-2235(96)00011-7","DOIUrl":"10.1016/0955-2235(96)00011-7","url":null,"abstract":"<div><p>The insulin-like growth factor binding proteins (IGFBPs) have conserved characteristics of their genomic organization, including similar locations of exon borders relative to nucleotides encoding conserved cysteine residues. Furthermore, the human IGFBP genes, as well as the human homeobox (HOX) genes, are localized to chromosomes 2, 7, 12, and 17. Although little is known about the evolution of the IGFBP genes, the association of human IGFBP and homeobox (HOX) genes at four chromosomal loci may indicate that their ancestral genes were linked prior to the first duplication of chromosomal DNA containing the ancestral HOX cluster. The hypothesis that IGFBPs are ancient proteins is supported by the reported detection of IGFBP activity in serum from the Agnathan species, <em>Geotria australis</em>, a primitive vertebrate.</p><p>Further studies of IGFBPs in different species are needed to understand the evolution of this protein/gene family. Chicken provides a good intermediate model, since birds diverged from mammals ∼300 million years ago. A complementary DNA (cDNA) clone encoding chicken insulin-like growth factor binding protein-5 (cIGFBP-5) was isolated. The deduced amino acid sequence is 83% identical to human IGFBP-5 and encodes a mature polypeptide of 251 amino acids. The conservation of IGFBP-5 primary structure across vertebrate species suggests maintenance of important functions during evolution.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 159-165"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(96)00011-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19786811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Serum proteolysis of IGFBP-3 IGFBP-3的血清蛋白水解

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(96)00007-5

Peter Bang

引用次数: 32

Clinical information on serum IGFBP-3 levels and IGFBP-3 proteolytic activity in childhood 儿童血清IGFBP-3水平和IGFBP-3蛋白水解活性的临床信息

Progress in growth factor research Pub Date : 1995-01-01 DOI: 10.1016/0955-2235(96)00005-1

Yukihiro Hasegawa , Tomonobu Hasegawa , Katsura Fujii , Hideko Konii , Makoto Anzo , Taiji Aso , Shinobu Kotoh , Yutaka Tsuchiya

引用次数: 4