发布求助
文献互助 智能选刊 最新文献

Agricultural and biological chemistry最新文献

筛选
英文 中文
Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene. 含潮霉素B抗性基因质粒对荨麻疹青霉的转化。
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB1961.55.3053
N. Kiuchi, A. Naruse, H. Yamamoto, J. Sekiguchi
{"title":"Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene.","authors":"N. Kiuchi, A. Naruse, H. Yamamoto, J. Sekiguchi","doi":"10.1271/BBB1961.55.3053","DOIUrl":"https://doi.org/10.1271/BBB1961.55.3053","url":null,"abstract":"A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"4 1","pages":"3053-7"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78532812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Effects of Administration of an Extract of Tu-chung Leaf (Eucommia ulmoides, Oliver) on Muscle Protein Synthesis in Mice 杜仲叶提取物对小鼠肌肉蛋白合成的影响
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB1961.55.3133
H. Yokogoshi, K. Hayase, J. Yamazaki, K. Koike, Shushichi Takahashi
{"title":"Effects of Administration of an Extract of Tu-chung Leaf (Eucommia ulmoides, Oliver) on Muscle Protein Synthesis in Mice","authors":"H. Yokogoshi, K. Hayase, J. Yamazaki, K. Koike, Shushichi Takahashi","doi":"10.1271/BBB1961.55.3133","DOIUrl":"https://doi.org/10.1271/BBB1961.55.3133","url":null,"abstract":"oldest herbs known and, from ancient times, its medical uses -such as to strengthen the intestinal organs and heart, to provide vigor of spirit, to remove fatigue, to prevent aging, and to strengthen bone and muscleshave been noted in China.l) However, its precise metabolic effects were not clear. Several investigators have confirmed the hypotensive action of aqueous and ethanol extracts of Tu-chung bark in various animals.2~4) It was considered that the systemic arterial hypotension caused by the Tuchung extract was peripheral vasodilation by its direct action on vascular smooth muscle.4) Manyappreciably morpho-pharmacological changes were also observed by feeding a water extract of Tu-chung.5) Takahashi et al.6) reported that in white laying hens the average oviposition rate of the group given Tu-chung leaves was improved, and also the contents of protein and RNA in the liver and muscle were significantly higher than those of hens 3133","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"25 1","pages":"3133-3134"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81465057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Involvement of NH2-terminal pro-sequence in the production of active aqualyson I (a thermophilic serine protease) in escherichia coli nh2末端前序列参与大肠杆菌活性酶I(一种嗜热丝氨酸蛋白酶)的产生
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1080/00021369.1991.10857921
Young-Choon Lee, Y. Miyata, I. Terada, T. Ohta, H. Matsuzawa
{"title":"Involvement of NH2-terminal pro-sequence in the production of active aqualyson I (a thermophilic serine protease) in escherichia coli","authors":"Young-Choon Lee, Y. Miyata, I. Terada, T. Ohta, H. Matsuzawa","doi":"10.1080/00021369.1991.10857921","DOIUrl":"https://doi.org/10.1080/00021369.1991.10857921","url":null,"abstract":"","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"29 1","pages":"3027-3032"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86144857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Purification and properties of thermostable tryptophanase from an obligately symbiotic thermophile, Symbiobacterium thermophilum. 嗜热共生菌嗜热共生菌中耐热色氨酸酶的纯化及性质研究。
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB.56.84
S. Suzuki, T. Hirahara, S. Horinouchi, T. Beppu
{"title":"Purification and properties of thermostable tryptophanase from an obligately symbiotic thermophile, Symbiobacterium thermophilum.","authors":"S. Suzuki, T. Hirahara, S. Horinouchi, T. Beppu","doi":"10.1271/BBB.56.84","DOIUrl":"https://doi.org/10.1271/BBB.56.84","url":null,"abstract":"A thermostable tryptophanase was extracted from a thermophilic bacterium, Symbiobacterium thermophilum strain T, which is obligately symbiotic with the thermophilic Bacillus strain S. The enzyme was purified 21-fold to homogeneity with 19% recovery by a series of chromatographies using anion-exchange, hydroxylapatite, hydrophobic interaction, and MonoQ anion-exchange columns. The molecular weight of the purified enzyme was estimated to be approximately 210,000 by gel filtration, while the molecular weight of its subunit was 46,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which indicates that the native enzyme is composed of four homologous subunits. The isoelectric point of the enzyme was 4.9. The tryptophanase was stable to heating at 65 degrees C for 20 min and the optimum temperature for the enzyme activity for 20 min reaction was 70 degrees C. The optimum pH was 7.0. The NH2-terminal amino acid sequence of this tryptophanase shows similarity to that of Escherichia coli K-12, despite a great difference in the thermostability of these two enzymes. The purified enzyme catalyzed the degradation (alpha, beta-elimination) of L-tryptophan into indole, pyruvate, and ammonia in the presence of pyridoxal-5'-phosphate. The Km value for L-tryptophan was 1.47 mM. 5-Hydroxy-L-tryptophan, 5-methyl-DL-tryptophan, L-cysteine, S-methyl-L-cysteine, and L-serine were also used as substrates and converted to pyruvate. The reverse reaction of alpha, beta-elimination of this tryptophanase produced L-tryptophan from indole and pyruvate in the presence of a high concentration of ammonium acetate.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"112 1","pages":"3059-66"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80674767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Involvement of NH2-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli. nh2末端前序列参与大肠杆菌活性溶酶I(一种嗜热丝氨酸蛋白酶)的产生。
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB1961.55.3027
Y. C. Lee, Y. Miyata, I. Terada, T. Ohta, H. Matsuzawa
{"title":"Involvement of NH2-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli.","authors":"Y. C. Lee, Y. Miyata, I. Terada, T. Ohta, H. Matsuzawa","doi":"10.1271/BBB1961.55.3027","DOIUrl":"https://doi.org/10.1271/BBB1961.55.3027","url":null,"abstract":"Aqualysin I is a heat-stable subtilisin-type protease produced by Thermus aquaticus YT-1. The precursor of aqualysin I consists of four domains: an NH2-terminal signal peptide, an NH2-terminal pro-sequence, a protease domain, and a COOH-terminal pro-sequence. In Escherichia coli cells harboring recombinant plasmid carrying the aqualysin I gene, proteolytic activity is obtained on treatment at 65 degrees C and mature enzyme is detected. In the case of mutant genes containing partial deletions in the NH2-terminal pro-sequence, no proteolytic activity was detected and the precursor protein was found to be unstable in E. coli. These results indicate that the NH2-terminal pro-sequence is required to produce the active enzyme by stabilizing the precursor structure. Amino acid substitutions in the conserved sequence of the NH2-terminal pro-sequence found among subtilisin-type proteases made the processing faster compared with the wild type.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"56 1","pages":"3027-32"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89230348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
Okaramine C, a New Insecticidal Indole Alkaloid from Penicillium simplicissimum 单纯青霉的一种新的杀虫吲哚生物碱——卡拉胺C
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB1961.55.3143
H. Hayashi, T. Fujiwara, Sawao Murao, M. Arai
{"title":"Okaramine C, a New Insecticidal Indole Alkaloid from Penicillium simplicissimum","authors":"H. Hayashi, T. Fujiwara, Sawao Murao, M. Arai","doi":"10.1271/BBB1961.55.3143","DOIUrl":"https://doi.org/10.1271/BBB1961.55.3143","url":null,"abstract":"14'-CH3 1.65 s. 1.52 s. insecticidal activity toward the 3rd instar larvae of silkworm upon oral administration. One of them, okaramine A (1),1>3) is a new type ofindole alkaloid with a seven-ring system, the other one, okaramine B (2),2'3) being a derivative with a eitht-ring system. Since we were interested in the unique structure of okaramines, we tried to determine if okaramines or similar compounds were produced by other strains. As a result, Penicillium simplicissimum AHU8402 showed activity almost equivalent to AK-40, the producing strain of okaramines.4) In this paper, we report the isolation and structural elucidation of the insecticidal constituents of AHU 8402. Froman acetone extract of mycelia and media of this strain, three insecticidal compounds were isolated. Twoof them were identified as okaramine A (1) and okaramine B (2), and the third one seemed to be a new congener, which was termed okaramine C (3). High-resolution El mass spectrometry of 3 established its molecular formula as C32H36N4O3. The 1HNMR(Table I) and 13C-NMR(Table II) spectra showed","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"68 1","pages":"3143-3145"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76797326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 30
NifA protein synthesized in vitro binds to the upstream activator sequence in the promoter of the Klebsiella oxytoca nifB gene. 体外合成的NifA蛋白结合克雷伯菌氧化克雷伯菌nifB基因启动子上游激活子序列。
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB1961.55.3121
Y. M. Kim, M. Hidaka, H. Masaki, T. Beppu, T. Uozumi
{"title":"NifA protein synthesized in vitro binds to the upstream activator sequence in the promoter of the Klebsiella oxytoca nifB gene.","authors":"Y. M. Kim, M. Hidaka, H. Masaki, T. Beppu, T. Uozumi","doi":"10.1271/BBB1961.55.3121","DOIUrl":"https://doi.org/10.1271/BBB1961.55.3121","url":null,"abstract":"The nitrogen fixation (nif) genes of various bacteria are transcribed with an RNApolymerase modulated by the alternative activated by the NifA protein.1* Several analyses of the Klebsiella pneumoniae nifYL promoter2'3) showed that not only binding of the NifA protein to the upstream activator sequence integration host factor (IHF) to the promoter region was essential for completetranscriptional activation. Here we demonstrate the binding ofKlebsiella oxytoca NifA protein synthesized in vitro to the UASof ni/B promoter and the interaction of IHF with the nifB promoter. The NifA protein was synthesized by a coupled transcription and translation4* of an artificial nifA gene, whose expression wascontrolled by the promoter of the chlor-amphenicol acetyl transferase gene, on pNOA1015)using S30 extract of Escherichia coli MRE600purchased from Amersham. To assay its DNAbinding activity, this trans-lation mixture was directly incubated with a DNAprobe","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"2 1","pages":"3121-3"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73408300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Regioselective modification of Lysine's amino groups by proteases. 蛋白酶对赖氨酸氨基的区域选择性修饰。
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1080/00021369.1991.10857914
H. Kitaguchi, M. Ono, I. Itoh, A. Klibanov
{"title":"Regioselective modification of Lysine's amino groups by proteases.","authors":"H. Kitaguchi, M. Ono, I. Itoh, A. Klibanov","doi":"10.1080/00021369.1991.10857914","DOIUrl":"https://doi.org/10.1080/00021369.1991.10857914","url":null,"abstract":"The regioselectivity of six serine proteases for the amino groups of lysine was investigated. alpha-Chymotrypsin showed a preference for the alpha-amino group, although the selectivity can be varied 10-fold depending on the reaction medium. Subtilisin Carlsberg and other bacterial proteases were highly specific for the epsilon-amino group, regardless of the reaction medium: they were used as catalysts for the preparative synthesis of isopeptides in anhydrous tert-amyl alcohol.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"26 1","pages":"3067-73"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83728034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
An Efficient Synthesis of (±)-α-Kainic Acid Using a 3-HydroxyalIylglycine Derivative as a Common Building Block 以3-羟基丙基甘氨酸衍生物为基础高效合成(±)-α-Kainic酸
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1271/BBB1961.55.3033
M. Kirihata, T. Kaziwara, Y. Kawashima, I. Ichimoto
{"title":"An Efficient Synthesis of (±)-α-Kainic Acid Using a 3-HydroxyalIylglycine Derivative as a Common Building Block","authors":"M. Kirihata, T. Kaziwara, Y. Kawashima, I. Ichimoto","doi":"10.1271/BBB1961.55.3033","DOIUrl":"https://doi.org/10.1271/BBB1961.55.3033","url":null,"abstract":"( + )-α-Kainic acid (1) was synthesized by starting from a building block, N-Boc-3-acetoxyallylglycine ethyl ester (2). The key intermediate, a methyl 4-[(tert-butoxycarbonyl)prenylamino]-5-hydroxy-2-pentenoate derivative (9), was prepared from 2 in eight synthetic steps. After converting 10 into a methyl ester (11), intramolecular ene-carbocyclization of 11 gave a pyrrolidine derivative (12), which was converted to 1 in a moderate yield.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"4 1","pages":"3033-3037"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88590101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Optical resolution and biological activities of S-23121, a new cereal herbicide 新型谷物除草剂S-23121的光学分辨率和生物活性
Agricultural and biological chemistry Pub Date : 1991-12-01 DOI: 10.1080/00021369.1991.10867925
M. Okamoto, R. Sato, E. Nagano, H. Nakazawa
{"title":"Optical resolution and biological activities of S-23121, a new cereal herbicide","authors":"M. Okamoto, R. Sato, E. Nagano, H. Nakazawa","doi":"10.1080/00021369.1991.10867925","DOIUrl":"https://doi.org/10.1080/00021369.1991.10867925","url":null,"abstract":"trolling many broadleaved weeds.1} Like Nphenylimide herbicide S-23142, in which the l-methyl-2-propynyl of S-23121 is substituted by 2-propynyl, S-23121 induces an abnormal accumulation of protoporphyrin IX, leading to lipid peroxydation.1} S-23121, having a chiral center at C-l in its chemical structure, is an optically active compound. Antipodes of bioactive chiral molecules are often thought to show quite different biological activity. It is, therefore, of great interest to know whether both enantiomers of S-23121 show similar","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"15 12 1","pages":"3151-3153"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86965932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信