{"title":"含潮霉素B抗性基因质粒对荨麻疹青霉的转化。","authors":"N. Kiuchi, A. Naruse, H. Yamamoto, J. Sekiguchi","doi":"10.1271/BBB1961.55.3053","DOIUrl":null,"url":null,"abstract":"A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"4 1","pages":"3053-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene.\",\"authors\":\"N. Kiuchi, A. Naruse, H. Yamamoto, J. Sekiguchi\",\"doi\":\"10.1271/BBB1961.55.3053\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.\",\"PeriodicalId\":7729,\"journal\":{\"name\":\"Agricultural and biological chemistry\",\"volume\":\"4 1\",\"pages\":\"3053-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural and biological chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1271/BBB1961.55.3053\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural and biological chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1271/BBB1961.55.3053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene.
A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.
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