发布求助
文献互助 智能选刊 最新文献

Microbiologica最新文献

筛选
英文 中文
Characterization of Newcastle disease viruses isolated from pigeons in Italy. 从意大利鸽子中分离出的新城疫病毒的特性。
Microbiologica Pub Date : 1991-07-01
C Buonavoglia, L Di Trani, D Buonavoglia, M Tempesta, F Marsilio
{"title":"Characterization of Newcastle disease viruses isolated from pigeons in Italy.","authors":"C Buonavoglia,&nbsp;L Di Trani,&nbsp;D Buonavoglia,&nbsp;M Tempesta,&nbsp;F Marsilio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors report the results of characterization studies of three strains of Newcastle disease virus (NDV) (two isolated from pigeons and one from chickens). The plaque cloning of the viruses, showed that each NDV strain consists of different clones of genetically mixed viral populations. The pigeon NDV isolates were classified as lentogenic using mean death time (MDT) determination; while the intracerebral pathogenicity index (ICPI) was the same as the velogenic NDV strain.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"253-6"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13080831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sexually transmitted diseases: advances in diagnostic biotechnologies. A brief overview. 性传播疾病:诊断生物技术的进展。简要概述。
Microbiologica Pub Date : 1991-07-01
M La Placa, M C Re, M La Placa, R Cevenini
{"title":"Sexually transmitted diseases: advances in diagnostic biotechnologies. A brief overview.","authors":"M La Placa,&nbsp;M C Re,&nbsp;M La Placa,&nbsp;R Cevenini","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"267-78"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13080834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary evaluation of sensititre system for identifying gram-negative bacilli. 革兰氏阴性杆菌敏感系统的初步评价。
Microbiologica Pub Date : 1991-07-01
G Mucignat, G L Tarabini-Castellani, L Pascoli, W De Cal, G Bruschetta, P De Paoli, G Santini
{"title":"Preliminary evaluation of sensititre system for identifying gram-negative bacilli.","authors":"G Mucignat,&nbsp;G L Tarabini-Castellani,&nbsp;L Pascoli,&nbsp;W De Cal,&nbsp;G Bruschetta,&nbsp;P De Paoli,&nbsp;G Santini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The automated microdilution Sensititre System (Sensititre, LtD.) was evaluated for the identification of 120 clinically isolated fermenter and non-fermenter Gram negative bacilli and of 12 ATCC reference strains (American Type Culture Collection). Daily and overnight (5 and 18 hours) identifications were performed according to the manufacturer. In both cases, the results were compared with those obtained with the overnight API 20E (API System, La Balme les Grottes, Montalieu Vercieu) used as reference method. Concordance between the results obtained after 5 hrs of incubation and those obtained with API 20E was found in 65% of cases (acceptable identification criteria with probability over 80%). When results were determined after 18 hrs of incubation (identification criteria with probably over 80%) concordance was found in 82.5%. Reproducibility obtained by repeated (10 times) identification with four different strains was 100% both at 5 hrs and 18 hrs. Identification accuracy of the 12 ATCC strains was 8/12 (66.6%) after 5 hrs and 12/12 (100%) after 18 hrs.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"261-5"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13080833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Activation of interferon-inducible genes in vivo by synthetic double-stranded RNA, poly rI:rC. 合成双链RNA poly - rI:rC在体内激活干扰素诱导基因。
Microbiologica Pub Date : 1991-07-01
M Gariglio, S Panico, G Gribaudo, M G Martinotti, G Cavallo, S Landolfo
{"title":"Activation of interferon-inducible genes in vivo by synthetic double-stranded RNA, poly rI:rC.","authors":"M Gariglio,&nbsp;S Panico,&nbsp;G Gribaudo,&nbsp;M G Martinotti,&nbsp;G Cavallo,&nbsp;S Landolfo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We analyzed the effects of synthetic dsRNA (poly rI:rC) treatment on the expression in vivo of two interferon (IFN)-inducible genes. DBA/2 mice were injected i.p. with poly rI:rC and its effect on the levels of the following mRNAs was determined; 202, 2'-5' oligoadenylate synthetase (2-5A synthetase) and beta-actin. After poly rI:rC treatment the levels of the 202 and 2-5A synthetase mRNAs in the spleen and in bone marrow peaked between 12 and 24 h and decreased thereafter, whereas beta-actin levels remained unchained unchanged. Pretreatment of DBA/2 mice with sheep anti-murine IFN-alpha/beta antibodies before rI:rC injection strongly diminished the induction of 202 mRNA indicating that IFN-alpha/beta mediated this induction.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"179-83"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12881864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binding properties of monoclonal antibodies to rabies virus. 狂犬病毒单克隆抗体的结合特性。
Microbiologica Pub Date : 1991-07-01
M G Cusi, P E Valensin, M Tollis, L Bracci, S Petreni, P Soldani
{"title":"Binding properties of monoclonal antibodies to rabies virus.","authors":"M G Cusi,&nbsp;P E Valensin,&nbsp;M Tollis,&nbsp;L Bracci,&nbsp;S Petreni,&nbsp;P Soldani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The monoclonal antibodies (mAbs) obtained by immunizing mice with a tetradecapeptide corresponding to the 190-203 region of rabies virus glycoprotein, involved in binding to the acetylcholine receptor (AchR), displayed different specificities to different rabies virus strains. These mAbs, when used in immunofluorescence tests, allowed differentiation of wild rabies viruses from the attenuated ones.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"205-11"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13080965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Streptococcus mutans: classification in bacteriocin-types. 变形链球菌:细菌素类型的分类。
Microbiologica Pub Date : 1991-07-01
M Bondi, R G Neglia, P Messi, G Manicardi, U Fabio
{"title":"Streptococcus mutans: classification in bacteriocin-types.","authors":"M Bondi,&nbsp;R G Neglia,&nbsp;P Messi,&nbsp;G Manicardi,&nbsp;U Fabio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A sample of S. mutans bacteriocins was studied to obtain a useful outline of strain typing since their synthesis has proved stable and not under plasmidial control. The inhibiting effectiveness against 9 oral streptococci and the sensitivity of mutacins produced by 49 S. mutans strains to heat, chloroform and proteasic activity were evaluated. On the basis of our results the producing strains are classified into five different types. We examine the possibility of obtaining a useful typing with bacteriocins and we discuss the choice of the most suitable number of indicators to arrange the strains in a limited cluster number for epidemiological purpose, or to classify freshly isolated S. mutans strains into bacteriocin-types.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"223-8"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13080968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitivity and specificity of anti-HIV ELISA employing recombinant (p24, p66, gp120) and synthetic (gp41) viral antigenic peptides. 重组(p24, p66, gp120)和合成(gp41)病毒抗原肽抗hiv ELISA的敏感性和特异性
Microbiologica Pub Date : 1991-07-01
G Filice, L Soldini, P Orsolini, E Razzini, R Gulminetti, D Campisi, L Chiapparoli, E Cattaneo, G Achilli
{"title":"Sensitivity and specificity of anti-HIV ELISA employing recombinant (p24, p66, gp120) and synthetic (gp41) viral antigenic peptides.","authors":"G Filice,&nbsp;L Soldini,&nbsp;P Orsolini,&nbsp;E Razzini,&nbsp;R Gulminetti,&nbsp;D Campisi,&nbsp;L Chiapparoli,&nbsp;E Cattaneo,&nbsp;G Achilli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have developed two immunoassay systems, one designated HIV (p24, p66, gp41) ELISA that uses as antigens the immunodominant epitopes mixed from each of three major groups of HIV-1 proteins: the core (p24), the pol (p66) and the env (gp41) gene. The other immunoassay system consists of four separate ELISAs for detection of single antibodies to HIV gag gene (p24), HIV pol gene (p66) and HIV env gene (gp41 and gp120). In the present study 200 specimens from patients with AIDS and 200 specimens from patients with ARC were repeatedly positive by HIV (p24, p66, gp41) ELISA. 1425 specimens from HIV drug addicts positive at W.B. were positive at HIV (p24, gp41, p66) ELISA. In addition, 60 samples that were indeterminate by W.B., were repeatedly positive at HIV (p24, p66, gp41) ELISA. The sensitivity and specificity of HIV (p24, p66, gp41) is estimated to be 100%. In this study 1507 specimens from HIV drug addicts, positive at W.B., were all positive (more than one test positive) at HIV p24 ELISA, HIV gp41 ELISA, HIV p66 ELISA and HIV gp120 ELISA used in combination. 135 samples from HIV positive drug addicts, positive at standard ELISA but indeterminate at W.B., were positive by HIV p24 ELISA, HIV gp41 ELISA, HIV p66 ELISA and HIV gp120 ELISA using the same criteria as in W.B. interpretation. The specificity (defined in terms of percentage of non-reacting persons in a low risk population) of HIV p24 ELISA, HIV gp41 ELISA, HIV p66 ELISA, HIV gp120 ELISA is 100%. In this work we demonstrated that: a) HIV (p24, p66, gp41) ELISA could be used as an adjunct or reliable alternative to standard ELISA for detection or confirmation of HIV antibodies in human sera; b) the specificity and sensitivity of antibodies to p24, p66, gp41, gp120 by ELISA used alone and/or in combination, is equal to or greater than W.B.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12881865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Duration of immune protection after hepatitis B vaccine in newborns from HBsAg-positive carrier mothers. 乙肝病毒携带者母亲所生新生儿接种乙肝疫苗后的免疫保护时间
Microbiologica Pub Date : 1991-07-01
C Belloni, P Orsolini, L Soldini, D Campisi, A Chiesa, R Ciceri, G Filice
{"title":"Duration of immune protection after hepatitis B vaccine in newborns from HBsAg-positive carrier mothers.","authors":"C Belloni,&nbsp;P Orsolini,&nbsp;L Soldini,&nbsp;D Campisi,&nbsp;A Chiesa,&nbsp;R Ciceri,&nbsp;G Filice","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A group of 43 healthy neonates born from HBsAg-positive mothers were evaluated for the presence of protective HBsAb titre after administration of hepatitis B plasma-derived vaccine and hepatitis B hyperimmune-globulin. At 36 months of life we found that a significant number of children had a low HBsAb serum level. This suggests that a close serologic follow-up is necessary to evaluate the optimal timing for the administration of a booster dose of the vaccine.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 3","pages":"195-8"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12994716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HIV-1 infection and hematologic picture. HIV-1感染和血液学图像。
Microbiologica Pub Date : 1991-04-01
M C Re, G Zauli, G Furlini, M Giovannini, M La Placa
{"title":"HIV-1 infection and hematologic picture.","authors":"M C Re,&nbsp;G Zauli,&nbsp;G Furlini,&nbsp;M Giovannini,&nbsp;M La Placa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This review examines the wide spectrum of hematological abnormalities frequently observed in the peripheral blood and bone marrow of HIV-1 infected subjects. Several pathogenetic mechanisms have been implicated in the derangement of the hematopoietic system occurring during the course of HIV-1 infection: imbalance of the T-cell subpopulation (CD4/CD8 ratio), altered cytokine production by infected CD4+marrow cells, production of inhibitory factors by infected marrow stromal cells, antibody-mediated suppression of hematopoietic progenitors, direct infection of hematopoetic progenitors and/or precursors, and HIV-1 mediated suppression of CD34+ cell growth in the absence of a complete viral replication cycle. Each point is considered in the text, with particular attention to the mechanisms most likely to operate in the early stages of the syndrome.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 2","pages":"165-76"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13019134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum-mediated killing of Salmonella typhimurium and Escherichia coli mutants which share a different content of major proteins. 鼠伤寒沙门菌和大肠杆菌突变体的血清介导杀伤，它们具有不同的主要蛋白质含量。
Microbiologica Pub Date : 1991-04-01
F Galdiero, L Sommese, C Capasso, M Galdiero, M A Tufano
{"title":"Serum-mediated killing of Salmonella typhimurium and Escherichia coli mutants which share a different content of major proteins.","authors":"F Galdiero,&nbsp;L Sommese,&nbsp;C Capasso,&nbsp;M Galdiero,&nbsp;M A Tufano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Salmonella typhimurium and Escherichia coli K12 mutants of R chemotype, with varying contents of major proteins, were studied with respect to serum-mediated killing. The mutants demonstrated a different susceptibility to serum lytic action. These results were related to phospholipid and fatty acid content, as well as different physico-chemical surface properties, such as outer membrane fluidity. Tests were carried out on all parameters considered in the literature to demonstrate the resistance to complement. Our results showed that in sensitive strains such as Salmonella strains SH6261, SH6378, SH5551, SH6017 and E. coli PC0479 tests taken alone were not sufficient to explain the resistance to complement. Therefore, complement susceptibility is probably determined by many factors influencing the microheterogeneity of the membrane system.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"14 2","pages":"119-30"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13019131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信