发布求助
文献互助 智能选刊 最新文献

Microbiologica最新文献

筛选
英文 中文
A mechanism involved in the plaque enhancement effect of sodium thiosulfate for foot-and-mouth disease viruses. 硫代硫酸钠对口蹄疫病毒斑块增强作用的机制。
Microbiologica Pub Date : 1992-10-01
K Kadoi
{"title":"A mechanism involved in the plaque enhancement effect of sodium thiosulfate for foot-and-mouth disease viruses.","authors":"K Kadoi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A mechanism involved in the plaque enhancement effect of foot-and-mouth disease viruses (FMDV) by the addition of sodium thiosulfate (Hypo) in the agar overlay medium (AOM) previously reported was studied. It was experimentally proved that the diffusion of virus particles through agar overlay medium was enhanced when this salt was incorporated. Accordingly, the enlarged plaque formation was assumed to be caused by the enhanced diffusion of viral progenies produced in infectious centers during plaque assays.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 4","pages":"399-401"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12505584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Absence of HTLV-I/II infection in blood donors with positive and inconclusive HTLV-I/II serology. HTLV-I/II血清学阳性且不确定的献血者中没有HTLV-I/II感染。
Microbiologica Pub Date : 1992-10-01
M C Re, G Furlini, E Ramazzotti, M Vignoli, G Zauli, S Lolli, P Monari, D Belletti, A Nanetti, M La Placa
{"title":"Absence of HTLV-I/II infection in blood donors with positive and inconclusive HTLV-I/II serology.","authors":"M C Re,&nbsp;G Furlini,&nbsp;E Ramazzotti,&nbsp;M Vignoli,&nbsp;G Zauli,&nbsp;S Lolli,&nbsp;P Monari,&nbsp;D Belletti,&nbsp;A Nanetti,&nbsp;M La Placa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pathogenetic potential and the true extent of human T leukemia/lymphotropic virus type I (HTLV-I) and type II (HTLV-II) infection are unknown. To find out more about HTLV-I/II seroepidemiology and the risks of iatrogenic transmission, we performed a serological study, screening 4086 healthy blood donors. A surprisingly high percentage of serum reactivity to HTLV-I/II antigens was observed by commercial ELISA (2.08%) and immunoblotting (IB) (0.85%) analysis, although none of the samples satisfied the (IB) criteria for positivity based on detection of gag protein p24 and at least one env gene product, either gp46 or gp61/68. To clarify these inconclusive results, we performed polymerase chain reaction (PCR) analysis for HTLV-I and HTLV-II provirus detection in peripheral blood lymphocytes, obtained from individuals with an apparent pattern of seropositivity. The data obtained by PCR failed to reveal evidence of HTLV-I/II provirus integration in peripheral blood cells, ruling out the possibility of a viral infection in these cases, and pinpointing the limitations of both serological methods used. Our observations suggest that serological assays alone are not a reliable tool for blood donor screening of HTLV-I/II infection and raise the important question of interpreting inconclusive results.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 4","pages":"329-36"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12505581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of ethylenediamine di-o-hydroxyphenylacetic acid and transferrin on the growth of some bacterial strains in vitro. 乙二胺、二邻羟基苯乙酸和转铁蛋白对部分细菌体外生长的影响。
Microbiologica Pub Date : 1992-10-01
A A Salamah
{"title":"Effect of ethylenediamine di-o-hydroxyphenylacetic acid and transferrin on the growth of some bacterial strains in vitro.","authors":"A A Salamah","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ability of some bacterial strains to obtain iron from ethylenediamine di-o-hydroxyphenylacetic acid (EDDA) or iron-free transferrin, and accordingly grow in their presence, was studied. Growth of Staphylococcus aureus and Yersinia pseudotuberculosis was inhibited by EDDA or by iron-free transferrin. Growth of Streptococcus faecalis, however, was inhibited by iron-free transferrin, but not by EDDA. The other bacterial strains, i.e.; Escherichia coli, Salmonella typhimurium and Shigella dysenteriae were able to grow both in the presence of EDDA or iron-free transferrin. All of the above bacterial strains grow in the presence of iron-saturated transferrin which was not able to bind the iron of the medium and accordingly left the iron of the medium available to them.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 4","pages":"361-6"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12606210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular phenoloxidase activity of micromycetes from various taxonomic groups. 不同分类群微菌胞外酚氧化酶活性。
Microbiologica Pub Date : 1992-10-01
P Guiraud, F Seigle-Murandi, R Steiman, J L Benoit-Guyod
{"title":"Extracellular phenoloxidase activity of micromycetes from various taxonomic groups.","authors":"P Guiraud,&nbsp;F Seigle-Murandi,&nbsp;R Steiman,&nbsp;J L Benoit-Guyod","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ability of Micromycetes strains to produce extracellular phenoloxidases was examined on solid malt agar medium using ten different reagents. We established a POx index summarizing the global activity given by the ten reagents used. The results indicated a wide variability depending on the taxonomic groups, the genera and the species. Some groups were relatively homogeneous, either no and low producers of phenoloxidases (Yeasts, Zygomycetes, genera Aspergillus and Penicillium) or medium and high producers of phenoloxidases (Basidiomycetes, Coelomycetes, Tuberculariales and Dematiaceae), while other groups were very heterogeneous (Ascomycetes, Mucedinaceae). The POx index was significantly higher for strains recently isolated than for strains kept in the fungi collection for a long time.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 4","pages":"367-90"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12606211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of three methods for the concentration of poliovirus from oysters. 牡蛎中脊髓灰质炎病毒三种浓缩方法的评价。
Microbiologica Pub Date : 1992-10-01
N Bouchriti, S M Goyal
{"title":"Evaluation of three methods for the concentration of poliovirus from oysters.","authors":"N Bouchriti,&nbsp;S M Goyal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three methods for the concentration of poliovirus from oyster homogenates were compared. The adsorption-elution-precipitation method gave the lowest average virus recovery (24.1%), while the beef extract elution-acid precipitation method and the non-fat dry milk elution-acid precipitation methods gave recoveries of 47.2% and 39.6%, respectively. Although the overall recovery rates with these methods were lower than those reported in previous studies, recoveries of 40-47% obtained with the elution-precipitation methods used in the present study are considered to be above average in terms of recovery efficiency.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 4","pages":"403-8"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12505585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of three immunoassays for the rapid detection of bovine respiratory syncytial virus. 牛呼吸道合胞病毒三种快速检测方法的比较。
Microbiologica Pub Date : 1992-07-01
B E Lokensgard, S M Goyal, D A Krueger
{"title":"Comparison of three immunoassays for the rapid detection of bovine respiratory syncytial virus.","authors":"B E Lokensgard,&nbsp;S M Goyal,&nbsp;D A Krueger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three enzyme-linked immunosorbent assays (EIA) designed for the detection of human respiratory syncytial virus (RSV) were evaluated for the detection of bovine respiratory syncytial virus (BRSV) in bovine lungs and the results were compared with those obtained by a direct fluorescent antibody assay (DFA). The EIA tests used were Directigen EIA, Kallestad Pathfinder EIA, and Abbott RSV EIA. Homogenates of lung tissues obtained from 64 cattle that had died of respiratory disease were used; 32 were positive by DFA and 32 were negative. All EIA's varied in the amount of labor and time involved but their relative sensitivities were similar ranging between 59 and 66% when compared with DFA. The specificity of Pathfinder EIA was lower than those of the Directigen and Abbott tests. The overall agreement between the three EIA's and the DFA was 66-77% indicating that DFA is still the test of choice for detecting BRSV infection in lung tissues of cattle.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 3","pages":"259-64"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12670311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cefonicid potentiation of human macrophage activity. 头孢菌酸增强人巨噬细胞活性。
Microbiologica Pub Date : 1992-07-01
V Tullio, A M Cuffini, S Fazari, N A Carlone
{"title":"Cefonicid potentiation of human macrophage activity.","authors":"V Tullio,&nbsp;A M Cuffini,&nbsp;S Fazari,&nbsp;N A Carlone","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was undertaken to evaluate the in vitro effects of cefonicid on phagocyte functions such as phagocytosis and intracellular killing of phagocytosed bacteria. At concentrations of half the MIC cefonicid caused human macrophages to ingest and kill Klebsiella pneumoniae at a greater rate than did drug-free macrophages. Bacteria pretreated with subinhibitory concentrations of cefonicid became more susceptible to the phagocytic and bactericidal activity of macrophages than untreated microorganisms. Sub-MIC cefonicid pretreatment of macrophages did not reduce phagocytosis and killing, confirming the inability of beta-lactam antibiotics to cross biological membranes.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 3","pages":"219-26"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12670306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viral susceptibility of an established cell line of swine embryo kidney. 猪胚肾细胞系的病毒易感性。
Microbiologica Pub Date : 1992-07-01
K Kadoi
{"title":"Viral susceptibility of an established cell line of swine embryo kidney.","authors":"K Kadoi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The viral susceptibility of a cell line, named KSEK6, newly established from the kidney cortex of swine embryo was tested for the indication of CPE occurrences and also plaque formations. The multiplication of porcine adenoviruses was considerably high in the cells among the virus strains tested though plaques of these viruses were hardly visible under agar overlay medium. Two strains of swine enteroviruses, Aujeszky's disease virus and hemagglutinating encephalomyelitis virus also multiplied well in a similar order to those received in the other cells employed.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 3","pages":"313-7"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12497188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular cloning of a specific DNA probe for the identification of Bacillus licheniformis. 地衣芽孢杆菌特异性DNA探针的分子克隆。
Microbiologica Pub Date : 1992-07-01
C Bollet, C Vignoli, P De Micco
{"title":"Molecular cloning of a specific DNA probe for the identification of Bacillus licheniformis.","authors":"C Bollet,&nbsp;C Vignoli,&nbsp;P De Micco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacillus licheniformis has been found to be one of the dominant nosocomial species of Bacillus: laboratories dealing with nosocomial infections must be able to identify Bacillus up to the species level. To date, no DNA probes have been isolated for B. licheniformis although there is a clear need for a direct detection by polymerase chain reaction. The isolation of a B. licheniformis-specific DNA probe, as described in this paper, represents the first step toward accomplishing this goal.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 3","pages":"291-5"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12670312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laboratory diagnosis of rotavirus infection in diarrhoeal patients by immunoenzymatic and latex-agglutination assays. 免疫酶和乳胶凝集试验对腹泻患者轮状病毒感染的实验室诊断。
Microbiologica Pub Date : 1992-07-01
F M Ruggeri, M L Marziano, E Salvatori, R Bisicchia, U Scardellato, M Scagnelli, M L Modolo, G Santini, G Donelli
{"title":"Laboratory diagnosis of rotavirus infection in diarrhoeal patients by immunoenzymatic and latex-agglutination assays.","authors":"F M Ruggeri,&nbsp;M L Marziano,&nbsp;E Salvatori,&nbsp;R Bisicchia,&nbsp;U Scardellato,&nbsp;M Scagnelli,&nbsp;M L Modolo,&nbsp;G Santini,&nbsp;G Donelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One hundred stool samples from children with acute diarrhoea were examined by six commercial latex and immunoenzymatic assays for the diagnosis of rotavirus infection in four different laboratories. Samples were also analyzed by solid-phase immune electron microscopy using a rabbit anti-group A rotavirus antiserum. With electron microscopy as a basis for comparison, sensitivity and specificity for the latex and ELISA assays varied from 91.1 to 92.9% and from 94.2 to 99.4%, respectively. Statistically significant differences were revealed in the confirmation rate of electron microscopy-negative samples between different commercial assays. Significant variability was also found between results obtained by the laboratories taking part in the study.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 3","pages":"249-57"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12497186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信