American Journal of Plant Sciences最新文献

{"title":"Role of Carbon Sources on <i>in </i><i>Vitro</i> Plant Regeneration in Alfalfa (<i>Medicago sativa</i> L.)","authors":"Dipika Parajulee, Suma Basak, Lauren Brett Morton, Sarwan K. Dhir","doi":"10.4236/ajps.2025.166050","DOIUrl":"https://doi.org/10.4236/ajps.2025.166050","url":null,"abstract":"Carbon sources play a critical role in plant tissue culture. This study evaluates the effects of different carbon sources—including monosaccharide hexoses (fructose, glucose, and galactose), disaccharides (maltose, sucrose, and lactose), and sugar alcohols (mannitol, sorbitol, and glycerol)—at concentrations ranging from 1% - 5% on in vitro plant regeneration in alfalfa (Medicago sativa L.). All leaf explants successfully induced callus formation when cultured on Gamborg’s B5 (B5h) medium supplemented with either monosaccharides or disaccharides in darkness for three weeks. Similarly, somatic embryo induction and maturation were enhanced in media containing monosaccharides (85.4% - 100%) and disaccharides (97.5% - 100%). Among all treatments, maltose at 3% exhibited rapid progression to the globular stage, yielding a 96.9% embryo-to-shoot conversion rate and the lowest incidence of shoot loss. Regenerated plantlets were successfully acclimatized and transferred to soil, achieving survival rates ranging from 50% - 100%, depending on the carbon source. These findings underscore the importance of carbon source selection for optimizing somatic embryogenesis protocols and advancing large-scale micropropagation of alfalfa and related legumes.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"16 06","pages":"703-723"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scirp.org/pdf/ajps_2606116.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-Fertilizer Improved Oil Palm Seedling Growth","authors":"Gabriel Ddamulira, Fred Bwayo Masika, Amos Asiimwe, L. Zubairi, M. Otuba","doi":"10.4236/ajps.2024.157032","DOIUrl":"https://doi.org/10.4236/ajps.2024.157032","url":null,"abstract":"In most farming systems newly introduced commercial fertilizers to be accepted, adopted and used by farmers, their effectiveness and appropriate application rates must be exhibited. This study was conducted to validate the effect and rates of a bio-fertilizer (super agric) on oil palm seedling growth. The trial was laid out in a randomized complete block design (RCBD) with three application rates of 0, 4 and 8 ml/L of water (treatments) replicated thrice. Following the application of super agric to oil palm seedlings for a period of six months, observations drawn from the analysis of growth data were as follows: Three months after treatment, super agric significantly (P < 0.05) increased the height and breadth of oil palm seedling compared to those which were not applied with super agric. The results also showed that when super agric was applied at a rate of 4 ml/L, the leaf length and breadth were higher compared to the control were super agric was not used. Furthermore, applications of super agric improved oil palm seedling nitrogen uptake by 31% in treatment groups as compared to the control which explained the height and breadth increase in the oil palm seedlings that were applied with super agric. On the other hand, the effect of super agric application on phosphorus uptake by seedlings was not significant. Although the height, breadth, leaf width and length were all significantly affected by super agric application, the number of oil palm leaves and spears were not affected for the period the experiment was conducted. Six months after application of super agric the growth of oil palm seedlings was favorably impacted, hence we recommend super agric to be promoted among oil palm seedling growers.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"15 07","pages":"455-466"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scirp.org/pdf/ajps2024157_12605879.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reaction of Sorghum Differentials to Grain Mold Infection in Puerto Rico","authors":"Louis K. Prom, Hugo E. Cuevas","doi":"10.4236/ajps.2023.1411081","DOIUrl":"https://doi.org/10.4236/ajps.2023.1411081","url":null,"abstract":"Grain mold, associated with many fungi, is the most important disease of sorghum, causing both yield and quality losses. In this study, 23 sorghum differentials used in pathotype characterization of anthracnose and head smut pathogens were evaluated for grain mold resistance under favorable conditions in Isabela, Puerto Rico. Lines BTx643 and IS18760 exhibited the lowest grain mold severity, indicating that these two may possess genes for grain mold resistance. These two lines also recorded the highest germination rates 94.7% and 97.6%, respectively, and their seed weight was among the heaviest. In conclusion, these two lines can be utilized in breeding programs to develop grain mold-resistant hybrid lines.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135448896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"&amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Propagation of Three Strawberry Varieties and Field Evaluation","authors":"Sussmita Karmaker, Md. Mukhtar Hossain, Md. Aminul Hoque, Md. Abdul Kaium, Tabaraka Binte Ali, Musfika Ferdous","doi":"10.4236/ajps.2023.1411082","DOIUrl":"https://doi.org/10.4236/ajps.2023.1411082","url":null,"abstract":"","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135507471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Selection for Seedling Tiller Number in Perennial Biomass Feedstocks Translate to Yield and Quality Improvements in Mature Swards?","authors":"Robert B. Mitchell, Kenneth P. Vogel, Susan J. Tunnell, James L. Stubbendieck","doi":"10.4236/ajps.2023.1411083","DOIUrl":"https://doi.org/10.4236/ajps.2023.1411083","url":null,"abstract":"Breeding for seedling traits in herbaceous perennial biomass feedstocks that translate into increased biomass yield or quality in established swards could accelerate the development of perennial grass cultivars for bioenergy or forage. In previously reported research, breeding for single large tillers (ST) or multiple tillers (MT, ≥3) six weeks after planting for two generations in big bluestem (Andropogon gerardii Vitman) and switchgrass (Panicum virgatum L.) base populations produced ST and MT populations that differed significantly for seedling and mature plant traits including biomass yield in spaced planted nurseries. Our objective was to evaluate these ST and MT populations in sward trials to determine the effect of these genetic changes on biomass yield and quality when the plants were grown in competitive sward conditions. Big bluestem monocultures of the base, ST, and MT populations were evaluated at three locations in Nebraska in 2001 and 2002 as randomized complete block experiments with four replicates. Switchgrass monocultures of the base, ST, and MT populations were evaluated in 2003, 2004, and 2005 near Mead, NE as a randomized complete block with six replicates. In both big bluestem and switchgrass, the ST and MT populations did not consistently differ from the base population or each other for biomass yield or forage quality. These results demonstrate the importance of evaluating perennial grasses in sward trials and not relying solely on greenhouse-grown plants or space-planted nurseries to develop selection criteria and make selection decisions.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135505260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative Assessment of Postharvest Stomata and Chloroplast Degradation in Contrasting Abscission Resistant Balsam Fir (&amp;lt;i&amp;gt;Abies balsamea&amp;lt;/i&amp;gt; (L.) Mill.)","authors":"Gaye MacDonald, Rajasekaran R. Lada, C. D. Caldwell, Chibuike C. Udenigwe, Mason T. Maconald","doi":"10.4236/ajps.2023.149069","DOIUrl":"https://doi.org/10.4236/ajps.2023.149069","url":null,"abstract":"Balsam fir is an important Christmas tree species, especially in eastern Canada. The natural Christmas tree industry faces a challenge in postharvest needle abscission. Though there have been many studies describing the physiological triggers and consequences in postharvest balsam fir, there have been no studies describing morphological or ultrastructural changes. Therefore, the objective of this study was to examine changes in stomata and chloroplast of postharvest needles. Branches were collected from low and high needle abscission resistance balsam fir genotypes, placed in water, and displayed in typical household conditions for 11 weeks. Needle abscission, chlorophyll fluorescence, and water uptake were monitored throughout. Needles stomata and chloroplasts were examined under a scanning and transmission electron microscope, respectively, each week. All branches had increased abscission, decreased chlorophyll fluorescence, and decreased water uptake over time. Needle surfaces accumulated fungal hyphae, especially in stomata. Chloroplasts demonstrated some dysfunction within two weeks, with notable decreases in chloroplast starch and increases in plastoglobulins. Within several weeks thylakoid membranes had been dismantled as chloroplasts transformed into gerontoplasts. All biophysical and structural changes were more pronounced in low needle abscission resistant genotypes. This research identifies a potential role for needle fungi in postharvest needle abscission and confirms the postharvest senescence of chloroplasts. Though it was previously speculated that chloroplasts must senesce postharvest, this study identifies how quickly this process occurs and that it occurs at different rates in contrasting genotypes.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135700475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and Morphological Responses of Susceptible and Resistant Barleys to Bird Cherry-Oat Aphid Feeding","authors":"Rafael Hayashida, John Scott Armstrong, William Wyatt Hoback, Dolores Mornhinweg","doi":"10.4236/ajps.2023.1410076","DOIUrl":"https://doi.org/10.4236/ajps.2023.1410076","url":null,"abstract":"The bird cherry-oat aphid (Rhopalosiphum padi [Linnaeus, 1758]) is considered a key pest of cereal crops worldwide, causing direct damage through sap feeding and by acting as a vector for viral diseases. Managing aphids is challenging because of their biology and potential resistance to insecticides. Developing resistant barley genotypes is a sustainable strategy for managing BCOA. In this study, we assessed responses of susceptible “Morex” and resistant “BCO R001” barley, Hordeum vulgare L. genotypes to different initial BCOA densities (0, 50, 100 or 200 aphids.plant-1). Physiological and morphological parameters were measured weekly for four weeks after infestation. Chlorophyll content, photosynthetic rate, plant aerial fresh and dry weight were greater for the resistant cultivar at lower aphid abundances and up to three weeks after infestation. Carbon assimilation curves (A/Ci) of infested “BCO R001” were similar to controls 15 days post infestation, differing from Morex. However, BCOA infestation of 50 aphid.plant-1 for two weeks negatively impacted the fitness of both genotypes. Initial resistance by BCO R001 to BCOA infestation can allow growers and natural enemies more time contributing to more effective and sustainable management of BCOA infestations.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136207807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Genetic Divergence in Various Potato Genotypes Grown in Bangladesh through Different Traits Assessment","authors":"Md. Mukhtar Hossain, Md. Abdul Kaium, Md. Al Amin, Tabaraka Binte Ali, Nusrat Jahan, Abu Saleh Nasim","doi":"10.4236/ajps.2023.1411084","DOIUrl":"https://doi.org/10.4236/ajps.2023.1411084","url":null,"abstract":"The goal of the study was to quantify the genetic diversity of different potato varieties. Five groups were named to classify the 25 genres of potatoes as: Cluster I (Lalpakri, Diamond), Cluster II (Hagrai, Green Mountain, G.M.O., Elvera), Cluster III (Atlantic, Shepody, Raja, Fundy, Multa, Granulla, Shibilati, Japanese Red), Cluster IV (Atlas, Brondy, Yucon Gold, Monona, Petrones, Cheroki), and Cluster V (Calwhite, Prelude, Allblue, Russet, Burbank, TPS-67). The highest and lowest genotypes are found in Clusters III and I, respectively. The two groups’ maximum inter-cluster distance (the cluster’s distance between them) demonstrates the enormous diversity between Clusters II & III. Cluster III had the greatest intra-cluster distance (distance within a set), whereas Cluster V had the smallest. In most cases, the distance between gaps was more significant than the distance within the holes, showing greater genetic diversity between different groups’ genotypes. The highest, second highest, and third highest eigenvalues, accompanying the positive canonical values for Vectors I and II of three characteristics: average tuber weight in each plant, the tubers number in each plant, and the eyes number in each tuber, showed the most outstanding contribution to the complete difference between genotypes. Of the nine features, the smaller leaves number in each plant, the shorter plant height, the more minor genotypes, the fewer eyes per tube generated from Cluster I, the maximum height of the plants, the high-quality tubers in each plant, the tubers number in each plant, the fresh weight in each plant, and the leaf number in each plant from Cluster II could be chosen in the role of parents in this program for hybridization. Given the size of the genetic distance, the various characteristics that contribute to the overall difference, and the average population size, Hagrai, Green Mountain, O.M.G., Elvera Cluster II and Cluster I, Lalpakri, and Diamond genotypes can be regarded as parents of hybridization programs in the future. Thus, producers can get guidance to enhance genetic diversity by selecting materials from different relatives and reducing their vulnerability to diseases and climate change.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135610441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Plant Extracts on Morphology and Cultural Characteristics of &amp;lt;i&amp;gt;Bipolaris sorokiniana&amp;lt;/i&amp;gt;, Causing Black Point Disease of Wheat in Bangladesh","authors":"Dilruba Yesmin, Md. Abul Kalam Azad, F. M. Aminuzzaman, Md. Monirul Islam","doi":"10.4236/ajps.2023.1410079","DOIUrl":"https://doi.org/10.4236/ajps.2023.1410079","url":null,"abstract":"Black point of wheat caused by Bipolaris sorokiniana (Bs) is very destructive disease of wheat in Bangladesh and all over the world. The symptoms of diseases caused by Bipolaris sorokiniana can vary depending on the host plant and environmental conditions. The conidia of Bs germinate from two poles, which differ physiologically as indicated by the rate, growth character, of length, breadth and number of septa on different media. The aim of this study was to evaluate the efficacy of selected plant extracts on the morphology and cultural characteristics of the fungus Bipolaris sorokiniana causing black point disease in wheat crops in Bangladesh. The efficacy of fifteen plant extracts on the growth and characteristics of Bs was conducted in vitro in 2019-2020. The treatments were: Untreated control, T1—Black berry Leaves, T2—Guava Leaves, T3—Lantena camera leaves, T4—Eucalyptus Leaves, T5— Turmeric Leaves, T6—Khoksha Leaves, T7—Papaya leaves, T8-Gurlic Bulb, T9—Chili dust, T10—Nigella seeds, T11—Turmeric dust, T12—Cloves, T13— Bohera fruits, T14—Black pepper and T15—Neem leaves. After 15 days of inoculation of Bipolaris sorokiniana, colony color and shapes were Gerrish blackish, greenish blackish, greyish blackish and shapes were round or irregular. In our study, conidia color was dark brown to light brown, conidiophore color was brown, dark brown, grayish brown, dark olivaceous, light brown and conidia shapes were elliptical, oblong or slightly curved. The septation of conidia was 2 - 5 and highest septation was seen in T4 and T14 treatments. The highest conidial length was 17.79 μm recorded in T2 treatment and lowest was 6.62 μm T9 treatment where conidial breath was 8.27 μm in control and lowest was 3.79 μm in T8 treatment. Mycelial growth rate of Bipolaris sorokiniana was different in different treatments in different days after inoculation (DAI) where at 7 DAI, % reduction of mycelial growth over control was highest in Bohera and Neem Leaves treatments respectively.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"304 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135260975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Combined Application of Organic Farming Aid (OFA) and Inorganic Fertilizers on the Growth and Yield of Maize and Soil Microbial Properties in the Guinea Savannah Agro-Ecological Zone of Ghana","authors":"O. A. Amoako, J. Adjebeng-Danquah, R. Y. Agyare, E. K. Akley, H. Abeka, Julius Yirzagla, T. K. Tengey, P. Teinor, R. Alhassan, A. A. Ibrahim, C. Naapoal","doi":"10.4236/ajps.2023.1410080","DOIUrl":"https://doi.org/10.4236/ajps.2023.1410080","url":null,"abstract":"The aim of the study was to evaluate the effect of different rates of organic farming aid (OFA) and inorganic fertilizer on the productivity of maize, chemical and microbial properties of soil for higher economic value. Field experiments were conducted during the 2020 and 2021 cropping seasons at the research fields of CSIR-Savanna Agricultural Research Institute located at Nyankpala in the Guinea savannah agroecology of Ghana. The study consisted of five treatment combinations: full rate of OFA, full rate of NPK, 1/2 OFA + 1/2 NPK, full OFA + 1/2 NPK and a control (no OFA and no NPK) which were arranged in a randomized complete block design with four replications. Analysis of variance indicated significant (P 0.05) treatment and year interaction effect for all the growth parameters except for plant height, leaf area and leaf area index. Apart from hundred seed weight, treatment and year interaction effect for all the yield and yield components was significant (P 0.05). Application of full rate of NPK (90:60:60) resulted in the highest grain yield of 4960 kg·ha-1, however it was statistically similar to those obtained by the combined application of full rate of OFA (250 ml·ha-1) + 1/2 NPK and 1/2 OFA + 1/2 NPK with grain yield of 4856 kg·ha-1 and 4639 kg·ha-1 respectively. There was also a yield advantage of 197. 5%, 191.3%, 178.3 and 79.1% over the control for full NPK rate, full OFA rate + 1/2 NPK rate, 1/2 OFA rate + 1/2 NPK and full OFA rate respectively. Application of full OFA rate + 1/2 NPK enhanced soil basal respiration (evolved CO2) and mineralizable C, implying that, combination of full OFA rate and NPK fertilizer would be necessary to boost soil microbial activity and soil labile nutrient pool (labile C pool). This suggests that combined use of full OFA rate + 1/2 NPK fertilizer can be a better strategic tool for improving soil quality. The highest benefit cost ratios (BCR) of 2.58 and 3.77 were obtained following the application of full OFA rate + 1/2 NPK and 1/2 OFA rate + 1/2 NPK respectively. Hence, it could be concluded that complementary use of OFA and NPK is more profitable than using single inputs (either OFA or NPK). Thus, in promoting technology packages to farmers, development practitioners must carefully consider the complementary of inputs that are cost-effective but economically rewarding.","PeriodicalId":7726,"journal":{"name":"American Journal of Plant Sciences","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135262436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}