Matrix (Stuttgart, Germany). Supplement最新文献

Oxidant-mediated inactivation of TIMP. 氧化介导的TIMP失活。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

G P Stricklin, J R Hoidal

引用次数: 0

Oncogenic consequences of down-modulating TIMP expression in 3T3 cells with antisense RNA. 下调带反义RNA的3T3细胞中TIMP表达的致癌后果。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

D T Denhardt, R Khokha, S Yagel, C M Overall, R S Parhar

{"title":"Oncogenic consequences of down-modulating TIMP expression in 3T3 cells with antisense RNA.","authors":"D T Denhardt, R Khokha, S Yagel, C M Overall, R S Parhar","doi":"","DOIUrl":"","url":null,"abstract":"We have used recombinant DNA technology to engineer a set of murine 3T3 cell lines that vary in the extent to which they express TIMP, tissue inhibitor of metalloproteinases, and we have found that both invasiveness and tumorigenic potential are conferred when TIMP production is impaired. These cell clones were produced by transfecting immortal Swiss 3T3 cells with plasmid constructs capable of expressing antisense TIMP RNA under the control of the mouse metallothionein promoter (Khokha & Denhardt, AntiCancer Res. 7: 653-660, 1987). The ability of these cells to invade the amnion membrane in vitro was dependent upon metalloproteinase expression (Yagel et al., JNCI 81: 768-775, 1989). Cells expressing TIMP at a reduced level acquired the ability to form tumors in nude mice (Khokha et al. SCIENCE 243: 947-950, 1989). These results suggest not only that TIMP controls the invasive character of the immortal 3T3 cell, but also that it determines cellular tumorigenic potential in the mouse. We presume that these phenotypes are conferred as a consequence of a net increase in extracellular matrix metalloproteinase activity resulting from the reduced quantities of TIMP secreted. The lag in formation of tumors by the cells down-modulated for TIMP production suggests that further changes in gene expression may be necessary. In support of this hypothesis, recent experiments indicate that the expression of genes encoding one or more matrix metalloproteinases is increased in cell lines derived from tumors that have developed from the engineered cells. Thus, in the immortal 3T3 cell line, TIMP has the properties of a tumor-suppressor gene, or anti-oncogene.","PeriodicalId":77254,"journal":{"name":"Matrix (Stuttgart, Germany). Supplement","volume":"1 ","pages":"281-5"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12649208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metalloproteinases in regions of the embryonic chick tibiotarsus. 鸡胫跗胚胎区金属蛋白酶的研究。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

A Cole, K Kuettner, T Schmid

引用次数: 0

Plasmin activation of collagenase during ovulation in the perfused rat ovary. 灌注大鼠卵巢排卵期胶原酶的纤溶酶活化。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

T Mukaida, N Morioka, C Zhu, T A Butler, W J Lemaire, J F Woessner

引用次数: 0

Secretion of matrix metalloproteinases in cultured stromal cells isolated from a giant cell tumor of bone. 骨巨细胞瘤基质细胞中基质金属蛋白酶的分泌。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

Y Sasaguri, S Komiya, R Nakano, H Yanagi, S Fukuda, A Inoue, M Morimatsu

引用次数: 0

Synthetic triple helical models for the collagen cleavage site in interstitial collagens. 间质性胶原中胶原裂解部位的合成三螺旋模型。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

U B Goli, G B Fields, H E Van Wart

引用次数: 0

Comparative sequence specificities of human fibroblast and neutrophil matrix metalloproteinases. 人成纤维细胞和中性粒细胞基质金属蛋白酶的序列特异性比较。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

S J Netzel-Arnett, G B Fields, H Nagase, K Suzuki, W G Moore, H Birkedal-Hansen, H E Van Wart

引用次数: 0

Positive and negative regulation of collagenase gene expression. 胶原酶基因表达的正调控和负调控。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

C Jonat, B Stein, H Ponta, P Herrlich, H J Rahmsdorf

{"title":"Positive and negative regulation of collagenase gene expression.","authors":"C Jonat, B Stein, H Ponta, P Herrlich, H J Rahmsdorf","doi":"","DOIUrl":"","url":null,"abstract":"Transcription of the human collagenase I gene is induced by phorbol esters and repressed by glucocorticoids. Both types of regulation are mediated by the major enhancer element of the gene, which is localized between positions -73 and -65. The enhancer suffices to transmit positive and negative signals to a heterologous promoter or to a minimal promoter carrying only the TATA-box, both detected by the appearance of chloramphenicol-acetyl-transferase transcribed from the reporter gene linked to the promoters. Sequences 5' of the enhancer modulate its activity. Up- and down-regulation of gene constructs which contain only the collagenase enhancer linked to a heterologous promoter, are independent of ongoing protein synthesis, suggesting posttranslational modification of the transcription factor binding to the enhancer. Repression by glucocorticoids depends on an activated glucocorticoid receptor; a tenfold lower glucocorticoid concentration is needed for repression of the collagenase gene as compared to the activation of the mouse mammary tumor virus long terminal repeat. Immunoprecipitates of the dexamethasone receptor contain AP-1, suggesting a direct interaction of both transcription factors; this interaction may lead to the inactivation of AP-1. The action mechanism of phorbol esters and dexamethasone confirms the central role of AP-1 in proliferation control and tumor promotion. It appears that the most effective tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and the most effective anti-tumor promoter dexamethasone exert their action through the modulation of the same transcription factor.","PeriodicalId":77254,"journal":{"name":"Matrix (Stuttgart, Germany). Supplement","volume":"1 ","pages":"145-55"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12650834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human neutrophil collagenase. 人中性粒细胞胶原酶。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

H E Van Wart

引用次数: 0

Tetracyclines (TCs) inhibit matrix metalloproteinases (MMPs): in vivo effects in arthritic and diabetic rats and new in vitro studies. 四环素(TCs)抑制基质金属蛋白酶(MMPs):对关节炎和糖尿病大鼠的体内作用及新的体外研究。

Matrix (Stuttgart, Germany). Supplement Pub Date : 1992-01-01

L Golub, R Greenwald, N Ramamurthy, S Zucker, L Ramsammy, T McNamara

引用次数: 0