Lymphokine and cytokine research最新文献_第3页

Effects of interleukin-4 on antigen-specific antibody synthesis by lymphocytes from old and young adults. 白细胞介素-4对老年和青年淋巴细胞抗原特异性抗体合成的影响。

Lymphokine and cytokine research Pub Date : 1994-08-01

E A Burns, G D L'Hommedieu, J L Cunning, J S Goodwin

引用次数: 0

Effects of hypoxia on the cytotoxicity mediated by tumor necrosis factor-alpha. 缺氧对肿瘤坏死因子- α介导的细胞毒性的影响。

Lymphokine and cytokine research Pub Date : 1994-08-01

A Naldini, S Cesari, V Bocci

引用次数: 0

Development of glycosylated human interleukin-1 alpha, neoglyco IL-1 alpha, coupled with D-mannose dimer: synthesis and biological activities in vitro. 糖基化人白细胞介素-1 α、新糖基化人白细胞介素-1 α偶联d -甘露糖二聚体的研制:体外合成及生物活性。

Lymphokine and cytokine research Pub Date : 1994-08-01

Y Takei, K Wada, T Chiba, H Hayashi, H Ishihara, K Onozaki

{"title":"Development of glycosylated human interleukin-1 alpha, neoglyco IL-1 alpha, coupled with D-mannose dimer: synthesis and biological activities in vitro.","authors":"Y Takei, K Wada, T Chiba, H Hayashi, H Ishihara, K Onozaki","doi":"","DOIUrl":"","url":null,"abstract":"Interleukin 1 (IL-1) is a nonglycosylated cytokine with pleiotropic effects on various cell types. In order to investigate the effect of carbohydrate introduction on IL-1 activity and to develop IL-1 with less deleterious effects recombinant human IL-1 alpha was chemically coupled with mannose dimers, alpha-D-Man1-6-D-Man[Man2(alpha 1,6)] and alpha-D-Man1-4-D-Man[Man2(alpha 1,4)]. About 5 molecules of mannose dimers were introduced per molecule of IL-1. Anti-IL-1 alpha antibody reacted only weakly with the glycosylated IL-1s. Conversely, antibody against the mannose dimer reacted with only glycosylated IL-1. The effect of glycosylation on IL-1 activity was evaluated by measuring a variety of IL-1 activities in vitro, including proliferative effect on T cells, antiproliferative effect on melanoma cells, stimulatory effect on IL-6 synthesis by melanoma cells, and stimulatory effect on prostaglandin E2 synthesis by fibroblast cells. Glycosylated IL-1s exhibited reduced activities, which were 10-fold to more than several hundred-fold lower than those of the original IL-1 alpha depending upon different aspects of activities addressed. Man2(alpha 1,6)-introduced IL-1 exhibited lower activity than Man2(alpha 1,4)-introduced IL-1. The competitive binding of 125I-IL-1 alpha to mouse T cells with unlabeled IL-1s suggests that the reduced activity of glycosylated IL-1s is due, at least partially, to the decrease of their receptor binding abilities.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"13 4","pages":"265-70"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18994747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of cytokine expression in PB-3c mastocytes by IBMX and PMA. IBMX和PMA对PB-3c乳腺细胞细胞因子表达的调节。

Lymphokine and cytokine research Pub Date : 1994-08-01

S Hahn, C Moroni

{"title":"Modulation of cytokine expression in PB-3c mastocytes by IBMX and PMA.","authors":"S Hahn, C Moroni","doi":"","DOIUrl":"","url":null,"abstract":"Stimulation of mast cells, either via the IgE receptor or with calcium ionophore triggers the production of several cytokines, such as interleukins-3, -4, -5, and -6, and GM-CSF. In PB-3c mastocytes, ionophore-induced IL-3 and GM-CSF expression is primarily the result of mRNA stabilization, and is enhanced by oncogenic ras. Apart from mobilizing calcium, the IgE receptor activation leads to production of DAG and elevation of cAMP levels, thereby activating protein kinases C and A, respectively. The influence of these two secondary messengers on cytokine production was examined using the cAMP elevating agent IBMX, the phorbol ester PMA, and the staurosporine derivative CGP 41251, which preferentially inactivates PKC. IBMX was determined to be a potent coinducer of IL-3 expression, whereas elevation of IL-6 and GM-CSF was more pronounced in PMA-treated cells. Both PMA and IBMX were shown to act posttranscriptionally on IL-3, by extending the half-life of the mRNA. Ionophore-induced cytokine expression appears to require serine/threonine kinase activity, as it could be abolished by treatment with the drug CGP 41251. Our results therefore suggest that the factors regulating cytokine expression and mRNA stability are subject to regulation by serine/threonine phosphorylation.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"13 4","pages":"247-52"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18536602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of human recombinant granulocyte-macrophage colony-stimulating factor and IL-3 on the expression of surface markers of human monocyte-derived macrophages in long-term cultures. 人重组粒细胞-巨噬细胞集落刺激因子和IL-3对长期培养的人单核细胞源性巨噬细胞表面标志物表达的影响。

Lymphokine and cytokine research Pub Date : 1994-08-01

R Dimri, L Nissimov, Y Keisari

{"title":"Effect of human recombinant granulocyte-macrophage colony-stimulating factor and IL-3 on the expression of surface markers of human monocyte-derived macrophages in long-term cultures.","authors":"R Dimri, L Nissimov, Y Keisari","doi":"","DOIUrl":"","url":null,"abstract":"Granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-3, which are involved in the maturation of cell precursors in the bone marrow into granulocytes and macrophages, were found also in chronic inflammatory sites, and their production might be enhanced by inflammatory stimulants. These findings led us to examine the effect of human recombinant GM-CSF (hrGM-CSF) and hrIL-3 on the maturation of human peripheral blood monocytes in long-term tissue cultures and on the expression of functional membrane bound molecules. Adherent human peripheral blood monocytes cultured for 2 weeks in the presence of GM-CSF or IL-3 were examined for viability and adherence, expression of membranal HLA-DR, CD-14, and IL-1 alpha, and LPS triggered TNF-alpha production. GM-CSF and IL-3 treatment increased the viability of adherent cells after 2 weeks in culture, and elevated the expression of membranal HLA-DR, CD-14 (LPS receptor), and IL-1 alpha. Such treated macrophage cultures also showed elevated production of TNF-alpha. The results indicate that GM-CSF and IL-3 facilitate the long-term maturation of monocytes into macrophages, augment their capacity to bind LPS, and elevate the release of cytokines involved in inflammatory and granulomatous reactions.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"13 4","pages":"239-45"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18536601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct comparison of ELISPOT and ELISA-based assays for detection of individual cytokine-secreting cells. 直接比较ELISPOT和elisa法检测单个细胞因子分泌细胞。

Lymphokine and cytokine research Pub Date : 1994-08-01

S Tanguay, J J Killion

引用次数: 0

Cloning and expression of the cDNA for canine tumor necrosis factor-alpha in E. coli. 犬肿瘤坏死因子α cDNA的克隆及在大肠杆菌中的表达。

Lymphokine and cytokine research Pub Date : 1994-06-01

K Zucker, P Lu, L Fuller, D Asthana, V Esquenazi, J Miller

引用次数: 0

Effect of interleukin-2 on salivary gland morphology and function in mice. 白细胞介素-2对小鼠唾液腺形态和功能的影响。

Lymphokine and cytokine research Pub Date : 1994-06-01

Y Marmary, E Okon, J Katz, E Shiloni

引用次数: 0

Interleukin-2 killer cells: in vitro evaluation of combination with prothymosin alpha. 白细胞介素-2杀伤细胞:与原胸腺素联合的体外评价。

Lymphokine and cytokine research Pub Date : 1994-06-01

J L López-Rodríguez, O J Cordero, C Sarandeses, J Viñuela, M Nogueira

{"title":"Interleukin-2 killer cells: in vitro evaluation of combination with prothymosin alpha.","authors":"J L López-Rodríguez, O J Cordero, C Sarandeses, J Viñuela, M Nogueira","doi":"","DOIUrl":"","url":null,"abstract":"Interleukin-2 (IL-2) is a potent inducer of lymphokine-activated killer (LAK) activity against both NK-sensitive and NK-resistant tumor cell lines. IL-2 therapy has been associated with clinical responses, but these responses have occurred only with high, toxic doses of the cytokine. Since prothymosin alpha (ProT alpha) is able to enhance the spontaneous NK activity of cells from normal donors, we studied the effect of ProT alpha on LAK activity by IL-2. The lysis of K562 and Daudi cells by effector cells cultured with IL-2 was increased time dependently when cultures also contained ProT alpha. PBLC was separated by discontinuous density centrifugation in the LGL-enriched fractions. Within the CD16+ population, all effector cell precursors were CD2+, but the effector population after IL-2 or IL-2 + ProT alpha activation also contained CD16+CD2- cells; the CD2 molecule is thus indispensable for induction of LAK activity by IL-2 or IL-2 + ProT alpha but not for the action of activated LAK cells (or for the enhancing effect of ProT alpha). Within the CD3- CD16+ LGL population, 5 micrograms/ml ProT alpha plus 50 U/ml IL-2 was able to induce p70 IL-2R expression to a similar extent to 100 U/ml of IL-2. The use of ProT alpha to enhance the induction of LAK activity by IL-2 may be able to improve immunotherapy of cancer.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"13 3","pages":"175-82"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18947805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of the mouse IL-2 gene by okadaic acid: synergy with interleukin-1. 冈田酸对小鼠IL-2基因的激活:与白细胞介素-1的协同作用。

Lymphokine and cytokine research Pub Date : 1994-06-01

W Falk, K Stricker, G Praast, Y Tsukitani, P H Krammer, M Stoeck

{"title":"Activation of the mouse IL-2 gene by okadaic acid: synergy with interleukin-1.","authors":"W Falk, K Stricker, G Praast, Y Tsukitani, P H Krammer, M Stoeck","doi":"","DOIUrl":"","url":null,"abstract":"Interleukin-1 (IL-1) has potent immunoregulatory and inflammatory functions. Its activity is mediated by an 80-kDa receptor on the cell surface and leads to activation of other genes. The underlying molecular events are largely unknown. We investigated the role of phosphatases in activation of the IL-2 gene in EL4 thymoma cells. We found that the protein phosphatase PP1 and PP2A inhibitor okadaic acid (OA) alone was able to significantly stimulate IL-2 production by the IL-1-responsive EL4 subline EL4 5D3 and also by the IL-1-nonresponsive EL4 subline EL4D6/76. In the IL-1-responsive cell line OA strongly synergized with phorbol myristate acetate (PMA) and IL-1. In the IL-1-nonresponsive cell line OA synergized with PMA but not with IL-1. Under suboptimal conditions of PMA/OA synergy an additional synergistic effect of IL-1 was shown. This was true for IL-2 and IL-6 production. Sphingomyelinase or sphingosine had no detectable effect. The kinetics of OA- and PMA-induced expression of IL-2 mRNA and IL-2 protein was different. PMA induced maximal expression between 6 and 12 h and was almost undetectable at 24 h. OA-induced expression was first obvious at 12 h and continued longer than 36 h. In both cases IL-1 caused no shift in kinetics, but potentiated the effects of the different tumor promoters. Utilizing IL-2 promoter-CAT constructs we showed in transfection experiments that the synergistic effect was also evident on the transcriptional level. We conclude from the data that phosphatases play an important role for IL-2 expression and that IL-1 can use additional pathways of activation that are different from events induced by PMA or OA.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"13 3","pages":"167-74"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18947804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0