{"title":"Bioavailability of zinc in rats fed on tuna as a protein source in the diet.","authors":"M T García-Arias, A M Castrillón, M P Navarro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The objective of this study was to investigate the dietary zinc bioavailability in diets containing raw, cooked, steamed, and canned tuna as a protein source in the diet, and to evaluate different means of preparation, as well as various storage periods of the canned tuna, which was prepared according to industrial standards. Sterilized oil-free canned tuna was also made. The biological assays used for the study of the zinc utilization were carried out on Wistar rats fed with semi-synthetic diets for 12 days varying only in the protein source, which was casein-methionine or tuna provided as follows: raw, cooked in brine, steamed, sterilized with or without soybean oil, canned and stored for one or three years. Body weight, food intake, faecal and urinary zinc excretion, and zinc content in rat liver were monitored. No modifications were shown in food efficiency when diets including tuna were used, compared with those values from the casein-methionine diet. Thus, final body weight results were similar in all groups tested. Dietary zinc absorption was enhanced by consuming a raw white tuna diet. This beneficial effect disappeared after steaming or cooking in brine, but it was slightly recovered after sterilization with oil and storage of the canned tuna. Animals fed on the diets including the oil-free canned tuna, showed two or three times higher faecal and urinary zinc excretion than those fed on casein and canned tuna stored for 0, 1 or 3 years, reaching a negative balance. A negative effect was therefore found in both dietary and endogenous zinc utilization due to the consumption of oil-free canned tuna.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"7 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19384934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Review of publications.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"7 1","pages":"57-62"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19384939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A M Loipführer, A M Reichlmayr-Lais, M Kirchgessner
{"title":"Concentration of free calcium in erythrocytes of lead-depleted rats.","authors":"A M Loipführer, A M Reichlmayr-Lais, M Kirchgessner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The concentration of Ca(2+)-ions in erythrocytes of lead-depleted rats was estimated using the fluorescent Ca(2+)-indicator fura-2. The lead-depleted animals of the F1 generation showed an elevated Ca(2+)-concentration, whereas in the Po generation there was no difference in the Ca(2+)-concentration between depletion and control group. The elevation of Ca(2+)-ion concentration of lead-depleted rats could be caused by a reduction in Ca-Mg-ATPase activity in lead-depletion and could possibly lead to a decreased mean corpuscular volume (MCV) of erythrocytes.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"7 1","pages":"37-40"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19384935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Perinatal development of iron and antioxidant defence systems.","authors":"T Günther, V Höllriegl, J Vormann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The time course of malondialdehyde (MDA), Fe, vitamin E, superoxide dismutase (Cu, Zn-SOD) and glutathione peroxidase (GSH-Px) was measured in serum, liver, kidney and heart from rats between day 17 of gestation and day 20 after birth. MDA was higher in fetal than maternal tissues and showed a transient increase after birth. The low maternal MDA levels were reached later than 3 weeks after birth. Fe was higher in fetal than in maternal serum and liver. Five days after birth Fe drastically dropped in these tissues. The antioxidant factors vitamin E, Cu, Zn-SOD and GSH-Px were low in fetal tissues and rose after birth. It is discussed whether lipid peroxidation is increased in fetal tissues because of less-developed antioxidant defence mechanisms.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"7 1","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19384937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transplacental passage and fetal deposition of mercury after low-level exposure to methylmercury--effect of seleno-L-methionine.","authors":"J B Nielsen, O Andersen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Previous experimental studies on transplacental passage and possible fetotoxicity of methylmercury have almost exclusively used a single dosage or 2-4 repeated doses of mercury on specific days during gestation and often used at relatively high dose levels. In previous studies, selenium supplementation considerably increased the concentration of mercury in the blood of offspring after maternal exposure of rats to methylmercury, whereas whole-body retention and organ deposition of mercury in mice were unaffected. The present study in mice, which involved exposure for 5 weeks to a low dose of methylmercury in the drinking water (1 nmol/ml) before and during pregnancy, demonstrates that mercury is deposited in offspring both in utero and during lactation, and that transplacentally absorbed mercury is not, or only very slowly, excreted. Seleno-L-methionine increased the deposition of mercury in offspring, but the effect was due to slightly higher deposition in the dams. Selenomethionine significantly reduced the kidney deposition of mercury in offspring, whereas liver deposition of mercury was unaffected. These results indicate that interactions between organo-selenium compounds and methylmercury orally administered at low doses is of less importance than previously believed on the basis of experiments with higher doses of selenite injected or supplemented to the diet.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"6 4","pages":"227-32"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12478198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P Brätter, R Brunetto, H J Gramm, S Recknagel, H Siemes
{"title":"Detection by HPLC-ICP of metallothionein in serum of an epileptic child with valproate-associated hepatotoxicity.","authors":"P Brätter, R Brunetto, H J Gramm, S Recknagel, H Siemes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A patient who developed valproate-associated hepatotoxicity had significantly lower serum levels of total protein, albumin and selenium than the controls. This study shows that with the beginning of the hepatic coma metallothionein (MT) appeared in the serum mainly in the form of Zn-thionein, which altered the Zn distribution pattern of the serum in a characteristic manner. HPLC-ICP3 was successfully applied to the simultaneous speciation of elements and characterization of MT by the use of one gel permeation column.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"6 4","pages":"251-5"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12478092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Review of publications.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"6 4","pages":"257-61"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12478093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The human selenium status in Hungary.","authors":"G Alfthan, G Bogye, A Aro, J Feher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The selenium concentration of foods and of human blood varies widely between geographical areas depending on the availability of soil Se for plants. The mean serum Se concentration in Europe has been found to vary between 0.80-1.50 mumol/l and the mean Se concentration of wheat between 0.02-0.11 mg/kg. We estimated by various markers the Se status of Hungarians on whom data is lacking. Serum samples (n = 238) from healthy blood donors, and toenails (n = 132) and fingernails (n = 211) from different apparently healthy individuals residing in both urban and rural areas were collected from four districts in Hungary. Wheat samples (n = 29) as grains were obtained directly from farms representing the same areas. Serum Se was determined by electrothermal AAS and the other samples by acid-digestion fluorimetry. The accuracy of the methods was verified by analyzing external reference samples. The mean (+/- SD) serum Se concentration was 0.70 +/- 0.12 mumol/l, ranging between 0.41-1.18 mumol/l. The mean serum Se levels 0.87 mumol/l was significantly (p < 0.001) higher in the East and lower in the South (0.60 mumol/l) compared to the northern area 0.70 mumol/l. The mean (+/- SD) toenail Se concentration was 0.56 +/- 0.18 mg/kg. The Se level in the East was significantly (p < 0.01) higher than the level in the North, 0.68 mg/kg vs. 0.54 mg/kg, respectively, and lowest in the southern area, 0.40 mg/kg. The same pattern applied to fingernails. Wheat Se ranged from 5 to 235 micrograms/kg, with the median 34 micrograms/kg. The lowest Se concentrations were generally found in the South.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"6 4","pages":"233-8"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12478199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nickel-, chrom- and cobalt-concentrations in human tissue and body fluids of hip prosthesis patients.","authors":"F F Hennig, H J Raithel, K H Schaller, J R Döhler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The world-wide experience with millions of metallic implantations suggests the biocompatibility of modern alloys, commonly made of cobalt, chromium and nickel. There is, however, little information available on the internal metal exposure resulting from implants. In this study we assessed the metal concentrations in body fluids and tissue samples (muscle, bone) of patients who had undergone total hip replacement. Our patients were divided up into two groups. One group had firmly fixed implants two years after surgery. The other group had loose implants of the same Co-Cr-Mo alloy. Urine analyses revealed an increased renal elimination of nickel, chromium and cobalt. Cobalt and nickel exceeded the upper normal value. In serum the concentrations of nickel and chromium were normal or slightly elevated, the cobalt concentrations were significantly elevated. In some cases tissues adjacent to the implant showed extremely high concentrations of chromium and cobalt. This finding was also obtained in tissues that had no direct contact with the arthroplasty. The findings suggest that alloys of prostheses can undergo corrosion and release metal ions.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"6 4","pages":"239-43"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12478200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A M Loipführer, A M Reichlmayr-Lais, M Kirchgessner
{"title":"Concentration of free calcium in erythrocytes and liver mitochondria of zinc-depleted rats.","authors":"A M Loipführer, A M Reichlmayr-Lais, M Kirchgessner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The concentration of Ca(2+)-ions in mitochondria and erythrocytes of zinc-depleted rats was determined with the fluorescent Ca(2+)-indicator fura-2. The zinc-concentration of the diet did not affect the mitochondrial Ca(2+)-concentration. There was an elevation of 5% for the Ca(2+)-concentration in erythrocytes of zinc-depleted and pair-fed rats compared to ad libitum-fed animals. The cause of this elevation could be ATP-depletion because of restricted food intake.</p>","PeriodicalId":77233,"journal":{"name":"Journal of trace elements and electrolytes in health and disease","volume":"6 4","pages":"223-6"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12478197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}