{"title":"Inter-relationships among subgroups, serotypes, and electropherotypes of rotaviruses isolated from humans.","authors":"S M el-Assouli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In an epidemiological study of human rotavirus (HRV) infections in metro Jeddah, Saudi Arabia, the relationships among subgroups, serotypes, and RNA electropherotypes of the rotavirus isolates were investigated. Of the 523 rotavirus-positive stool specimens, 245 were examined for subgroup, serotype, and electropherotype. Of these, 84 isolates were analyzed for their subgroup and RNA electropherotype specificites, 12 (14.3%) were of subgroup 1, 69 (82.1%) were of subgroup II, and 3 (3.6%) were a mixture of subgroup I and II. Of the subgroup 1 specimens, 5 (41.7%) showed long electrophoretic migration patterns and 7 (58.3%) showed short patterns. In subgroup II specimens, 66 (95.7%) were of long patterns and 3 (4.3%) of short patterns. The relationship between HRVS serotypes and electropherotypes was also determined for the same 245 rotavirus specimens. Of these, 36 (14.7%) exhibited short RNA patterns and 209 (85.3%) exhibited long patterns. The short pattern specimens consisted of serotype 1 (8.3%), serotype 2 (63.9%), serotype 3 and serotype 4 (2.8%) each. The long pattern specimens consisted of serotype 1 (60.3%), serotype 2 (1.4%), serotype 3 (7.2%) and serotype 4 (17.7%). Among the previous 245 specimens, subgroup specificities were available for 51 specimens. All subgroup I were of serotype 2, and all subgroup II were of serotype 1, 3 or 4. RNAs of either subgroup showed both long and short electropherotypes. No relationship could be established between subgroups or serotypes and a particular electropherotype. It seems unlikely that electropherotyping of human rotavirus (HRV) can be used for identifying the subgroups or serotypes of strains.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"201-6"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Chaudhary, M Verma, V Dhawan, C K Nain, R Kumar, L Kumar, N K Ganguly
{"title":"Plasma vitamin A, zinc and selenium concentrations in children with acute and persistent diarrhoea.","authors":"S Chaudhary, M Verma, V Dhawan, C K Nain, R Kumar, L Kumar, N K Ganguly","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasma zinc, selenium, and vitamin A concentrations in 25 children with persistent diarrhoea (PD) and acute diarrhoea (AD) were determined and compared with 25 age-matched control children. Plasma retinol concentrations (PRC) and plasma zinc concentrations (PZC) (3.92 micrograms/dL and 79.4 micrograms/dL respectively) were found to be significantly lower (p < 0.05) in children with PD. PZCs were also significantly reduced (p < 0.05) in children with AD. However, reduction in PZC was more in PD than that in AD. There was no significant difference in PSC in children with either types of diarrhoea as compared with the control group. The results of the study showed that there was deficiency of vitamin A and zinc in diarrhoeas which needs to be correlated for proper nutritional management.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"190-3"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evidence for possible involvement of guanylate cyclase in diarrhoea induced by castor oil in mice.","authors":"F S Robert, J P Rao","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"218-9"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W K Sang, T Iida, H Yamamoto, S M Saidi, M Yoh, P G Waiyaki, T Ezaki, T Honda
{"title":"Prevalence of diarrhoeagenic Escherichia coli in Kenyan children.","authors":"W K Sang, T Iida, H Yamamoto, S M Saidi, M Yoh, P G Waiyaki, T Ezaki, T Honda","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"216-7"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J Singh, D Bora, K K Khanna, D C Jain, V Sachdeva, R S Sharma, T Verghese
{"title":"Epidemiology and transmission of V. cholerae O1 and V. cholerae O139 infections in Delhi in 1993.","authors":"J Singh, D Bora, K K Khanna, D C Jain, V Sachdeva, R S Sharma, T Verghese","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In 1993, rectal swabs from clinically suspected cases of cholera admitted to the Infectious Diseases Hospital (IDH), Delhi were examined for Vibrio cholerae O1 and O139. Epidemiological data of 396 cholera cases were collected before the patients' discharge from IDH. Of the 1528 laboratory-confirmed cholera cases, 46% and 54% were caused by serotype O1 and O139 respectively. Both serotypes appeared and disappeared simultaneously, and peaked during the same time of the year. However, the two serotypes affected persons of different age groups; about 65% of the O1 cases occurred in children aged less than 10 years, whereas this age group accounted for 40% of the cases due to V. cholerae O139. Although there were some focal outbreaks due to serotype O139, both serotypes had almost similar geographical distributions. Important risk factors for transmission of cholera were almost equally prevalent in the majority of both types of cholera cases. Since the seasonality, geographical distribution, and risk factors for transmission were similar for both serotypes, the study indicates that the preventive and control measures are also likely to be similar. The study also shows that the emergence of V. cholerae O139 in 1993 did not affect the incidence, seasonality, and epidemiology of endemic V. cholerae O1 E1 Tor strains in Delhi.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"182-6"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oscillatory fluctuations in the incidence of rotavirus infections by serotypes 1, 2, 3, and 4.","authors":"M V José, J R Bobadilla, R F Bishop","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The statistical evidence for regularity in the epidemic cycles of rotavirus infection for serotypes 1, 2, 3, and 4 was examined. Hospitalization longitudinal data of the monthly incidence of rotavirus infections from the city of Melbourne, Australia during 1977-1993 were used. Periodograms were used for exploring seasonal and longer-term cycles (interepidemic periods) of rotavirus infection. There was a satisfactory agreement between the interepidemic period estimated by means of periodograms with the one predicted by theoretical epidemiological studies. Thus, there is a clear evidence of a biennial peak in the epidemiology of rotavirus. Results of the study show an evidence of the likely existence of an interepidemic cycle of 4.6-5.2 years of duration. The finding of this interepidemic cycle was unexpected, and does not arise from the alternating incidence of the 4 serotypes since this peak appears in the periodogram of each serotype.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"194-200"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cryptosporidiosis among cancer patients: an observation.","authors":"A Sreedharan, R S Jayshree, H Sridhar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The incidence of cryptosporidiosis was studied in 560 cancer patients presenting with symptoms of diarrhoea. Cryptosporidium oocysts were detected in the stool specimens by modified acid fast staining. Blood was examined for HIV antibodies from patients whose stool specimens were positive for Cryptosporidium oocysts. Oocysts were detected in 7 of the 560 (1.3%) patients who were receiving chemotherapy and had diarrhoea; in five of them the symptoms were acute, while tow patients had chronic diarrhoea. Of these seven patients, five had cancer of haemopoetic system, one had cancer of cervix and one had uterine sarcoma. None of the patients positive for oocysts were positive for HIV antibodies, ruling out the possibility of an opportunistic infection due to HIV infection.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"211-3"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vibrio cholerae O139 in the subcontinent.","authors":"B N Shukla, G Nath, A Chaudhury, S C Sanyal","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"214-5"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Ceyhan, O Akan, G Kanra, Z Ecevit, G Seçmeer, E Berkman
{"title":"Changing patterns of the prevalence of different Shigella species and their antibiotic susceptibilities in Ankara, Turkey.","authors":"M Ceyhan, O Akan, G Kanra, Z Ecevit, G Seçmeer, E Berkman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Shigella flexneri was the most common Shigella serogroup isolated in Turkey. Recently, an increase in the number of Shigella sonnei isolates was noticed. A retrospective analysis of 2,710 isolates, obtained from stools of Turkish children between January 1980 and September 1994, revealed that, between 1980 and 1987, S. flexneri was the most common subgroup. The isolation rate of S. sonneri increased steadily from 1987 to 1994 reaching to a peak of 78% of all isolates in 1991. The antibiotic susceptibility of 206 strains isolated in 1994 was also studied. A marked difference between the two species was observed for chloramphenicol (98% susceptibility in S. sonnei versus 20% in S. flexneri, ampicillin (90% vs. 18%), ampicillin-sulbactam (98% vs. 53%), and tetracycline (46% vs. 18%) (p < 0.001). Susceptibility to trimethoprim-sulphamethoxazole was similar between the two groups (42% vs. 38%). All isolates were susceptible to ciprofloxacin and ceftriaxone. Comparing our results with resistance rates in 1989, a marked increase in amplicillin (from 44.1% to 82%), chloramphenicol (from 36.7% to 56%) and trimethoprim-sulphamethoxazole (from 35.8% to 62%) resistance was observed.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"187-9"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B A Oyofo, Z S Mohran, S H el-Etr, M O Wasfy, L F Peruski
{"title":"Detection of enterotoxigenic Escherichia coli, Shigella and Campylobacter spp. by multiplex PCR assay.","authors":"B A Oyofo, Z S Mohran, S H el-Etr, M O Wasfy, L F Peruski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three oligonucleotide primers were used in a polymerase chain reaction (PCR) assay for the simultaneous amplification of regions of the invasive plasmid antigen (ipaH) of Shigella spp., flagellin gene (flaA) of Campylobacter spp., and heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC). The multiplex assay was performed using DNA extracted by a chaotropic method directly from diarrhoeal stools. The diagnostic efficacy of the assay was analyzed by agarose gel electrophoresis. This assay shows a novel approach for the diagnosis of diarrhoea caused by Shigella spp., ETEC, and Campylobacter spp.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 3","pages":"207-10"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}