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Journal of diarrhoeal diseases research最新文献

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Detection of non-culturable Shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and PCR techniques. 应用荧光抗体和PCR技术检测人工污染志愿者手指中不可培养的志贺氏痢疾菌1。
Journal of diarrhoeal diseases research Pub Date : 1997-06-01 DOI: 10.1097/00005176-199808000-00050
M. S. Islam, Hossain Mz, S. Khan, A. Felsenstein, Sack Rb, Albert Mj
{"title":"Detection of non-culturable Shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and PCR techniques.","authors":"M. S. Islam, Hossain Mz, S. Khan, A. Felsenstein, Sack Rb, Albert Mj","doi":"10.1097/00005176-199808000-00050","DOIUrl":"https://doi.org/10.1097/00005176-199808000-00050","url":null,"abstract":"Epidemiological studies have demonstrated that hands may be an important vehicle for transmission of shigellosis. The present study was carried out to find out the survival potential of Shigella dysenteriae 1 on fingers of volunteers. Finger surface was inoculated with 10(5) cfu of S. dysenteriae 1 and then the bacteria were detected using conventional culture, PCR and fluorescent antibody (FA) techniques after different time intervals. It was found that S. dysenteriae 1 survived for up to one hour in culturable form but up to four hours in non-culturable form on human fingers. The non-culturable S. dysenteriae was detected by PCR and FA techniques. This study elaborates on the role that fingers have in the transmission of shigellae.","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 2 1","pages":"65-70"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61450253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Detection of non-culturable Shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and PCR techniques. 应用荧光抗体和PCR技术检测人工污染志愿者手指中不可培养的志贺氏痢疾菌1。
Journal of diarrhoeal diseases research Pub Date : 1997-06-01
M S Islam, M Z Hossain, S I Khan, A Felsenstein, R B Sack, M J Albert
{"title":"Detection of non-culturable Shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and PCR techniques.","authors":"M S Islam,&nbsp;M Z Hossain,&nbsp;S I Khan,&nbsp;A Felsenstein,&nbsp;R B Sack,&nbsp;M J Albert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Epidemiological studies have demonstrated that hands may be an important vehicle for transmission of shigellosis. The present study was carried out to find out the survival potential of Shigella dysenteriae 1 on fingers of volunteers. Finger surface was inoculated with 10(5) cfu of S. dysenteriae 1 and then the bacteria were detected using conventional culture, PCR and fluorescent antibody (FA) techniques after different time intervals. It was found that S. dysenteriae 1 survived for up to one hour in culturable form but up to four hours in non-culturable form on human fingers. The non-culturable S. dysenteriae was detected by PCR and FA techniques. This study elaborates on the role that fingers have in the transmission of shigellae.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 2","pages":"65-70"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20291379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RNA profile and structural protein analysis of rotaviruses isolated from diarrhoeal calves in India. 印度腹泻小牛分离的轮状病毒的RNA谱和结构蛋白分析。
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
B R Gulati, S Maherchandani, D P Patnayak, R Pandey
{"title":"RNA profile and structural protein analysis of rotaviruses isolated from diarrhoeal calves in India.","authors":"B R Gulati,&nbsp;S Maherchandani,&nbsp;D P Patnayak,&nbsp;R Pandey","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two isolates of group A rotaviruses (CR129 and CR156) were isolated from faecal samples of diarrhoeal calves reared in two dairy farms at Hisar (Haryana, India) by using MA-104 cell lines. These isolates were compared with three standard reference bovine rotaviruses, UK, NCDV and B223, to reveal differences, if any, in their genome and protein migration profiles. The migration of RNA segment 4 of CR129 was slower than that of NCDV, but faster than that of UK. Segment 10 of CR156 moved faster than that of the reference viruses. The segments 2 and 3 co-migrated in CR129, but resolved separately in CR156. Five protein bands of size 116-120 KD (VP1), 95 KD (VP2), 90 KD (VP3/VP4), 44 KD (VP6) and 34 KD (VP7) were detected by protein analysis. No significant difference was observed in the protein profile of these two bovine rotavirus isolates by immunoblotting. However, VP1 was of approximately 116 KD size in the two isolates, compared to 120 KD in the reference strains. These findings indicate that these rotaviruses isolated from diarrhoeic Indian calves differed from the 3 reference strains.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"12-6"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vibrio cholerae O1 and O139 in less than five years old children hospitalised for watery diarrhoea in Delhi, 1993. 1993年，德里因水样腹泻住院的5岁以下儿童体内的霍乱弧菌O1和O139。
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
J Singh, D Bora, V Sachdeva, R S Sharma, T Verghese
{"title":"Vibrio cholerae O1 and O139 in less than five years old children hospitalised for watery diarrhoea in Delhi, 1993.","authors":"J Singh,&nbsp;D Bora,&nbsp;V Sachdeva,&nbsp;R S Sharma,&nbsp;T Verghese","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In Delhi, patients with cholera-like illness are admitted to the Infectious Diseases Hospital. In 1993, rectal swabs from 836 such patients aged less than five years were examined for the presence of Vibrio cholerae O1 and O139. Of them, 232 (28%), 180 (22%), and 424 (51%) were found suffering from O1 cholera, O139 cholera, and non-cholera watery diarrhoea respectively. Twelve children (1.4%) excreted both V. cholerae O1 and O139. Both types of cholera were similarly distributed by age, with 19% of the cases occurring in infants. The findings indicate that cholera should be suspected in children aged less than two years and in infants with acute watery diarrhoea. For both serotypes, males were more represented than females; the differences were, however, not significant. Clinical features of patients with V. cholerae O139 and O1 were indistinguishable, except that a significantly higher percentage of the former had fever. Potential risk factors for cholera were almost equally prevalent in the families of children aged less than 5 years having either O1 or O139 cholera. The results suggest a similar mode of transmission of the two serotypes in children. By inference, the preventive and control measures are also likely to be similar.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"3-6"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reactive arthritis associated with Shigella dysenteriae type 1 infection. 反应性关节炎与志贺氏痢疾杆菌1型感染相关。
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
R N Mazumder, M A Salam, M Ali, M K Bhattacharya
{"title":"Reactive arthritis associated with Shigella dysenteriae type 1 infection.","authors":"R N Mazumder,&nbsp;M A Salam,&nbsp;M Ali,&nbsp;M K Bhattacharya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Shigella dysenteriae type 1 causes the most severe form of bacillary dysentery. The spectrum of illness ranges from mild watery diarrhoea to severe bloody diarrhoea. Shigellosis is often associated with intestinal complications, including intestinal perforation, intestinal obstruction, toxic dilatation of the colon, and prolapse of the rectum; systemic complications include septicaemia, hyponatraemia, hypoglycaemia, seizure, encephalopathy, haemolytic-uraemic syndrome, and malnutrition. Arthritis and conjunctivitis are rare extra-intestinal complications of shigellosis. Annually, about 110,000 patients receive treatment in the Dhaka Hospital of the International Centre for Diarrhoeal Disease Research, Bangladesh for diarrhoea and diarrhoea-associated illnesses, of which 11% are due to shigellosis. However, arthritis associated with shigellosis has not been reported from this population. Arthritis has been reported in association with infection due to S. flexneri and S. sonnei from other places. We are unaware of any reported case of arthritis in association with S. dysenteriae type 1 infections. In this report, we describe the clinical and laboratory features of a young woman who developed arthritis following S. dysenteriae type 1 infection.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"21-4"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does nitric oxide induce diarrhoea in mice? 一氧化氮会引起小鼠腹泻吗?
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
F S Robert, J P Rao
{"title":"Does nitric oxide induce diarrhoea in mice?","authors":"F S Robert,&nbsp;J P Rao","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"25-7"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiple drug-resistant Shigella dysenteriae type 1 in Rajbari district, Bangladesh. 孟加拉国Rajbari地区1型多重耐药志贺氏痢疾菌。
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
Y Jahan, A Hossain
{"title":"Multiple drug-resistant Shigella dysenteriae type 1 in Rajbari district, Bangladesh.","authors":"Y Jahan,&nbsp;A Hossain","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty-one Shigellae isolates were obtained from bloody faecal specimens of diarrhoeal patients at Rajbari District Hospital from January 1994 to June 1995, and serogrouped. Fourteen (67%) isolates belonged to the Shigella dysenteriae serogroup and 7 (33%) to Shigella flexneri serogroup. Shigella dysenteriae strains were further serotyped; all were Shigella dysenteriae 1. Each strain was tested for resistance to 6 common antimicrobial agents. The two strains had different antibiotic susceptibility patterns. The 7 S. flexneri showed 6 different resistant patterns and the 14 S. dysenteriae 1 isolates had 4 resistance patterns. One of the S. dysenteriae 1 isolates was resistant to all 6 antimicrobial agents; 10 to 5, and twice to a different combination of 4 antimicrobials. The 14 (100%) S. dysenteriae 1 strains were resistant to 3 major antimicrobial agents: ampicillin, tetracycline, and chloramphenicol; 13 (93%) were resistant to 5 agents: ampicillin, tetracycline, chloramphenicol, trimethoprim-sulphamethoxazole, and nalidixic acid. Ciprofloxacin was the only drug active against all 7 S. flexneri and 13 of the 14 (93%) S. dysenteriae 1 strains.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bibliography on diarrhoeal diseases. 关于腹泻疾病的参考书目。
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
M S Khan, M M Ali
{"title":"Bibliography on diarrhoeal diseases.","authors":"M S Khan,&nbsp;M M Ali","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"28-46"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low body weight: a simple indicator of the risk of dehydration among children with diarrhoea. 体重过轻:腹泻儿童脱水风险的一个简单指标。
Journal of diarrhoeal diseases research Pub Date : 1997-03-01
C G Victora, S C Fuchs, B R Kirkwood, C Lombardi, F C Barros
{"title":"Low body weight: a simple indicator of the risk of dehydration among children with diarrhoea.","authors":"C G Victora,&nbsp;S C Fuchs,&nbsp;B R Kirkwood,&nbsp;C Lombardi,&nbsp;F C Barros","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The early identification of children at high risk of dehydration as a consequence of diarrhoea would be of great value for health care workers in developing countries. By comparing children aged less than two years with diarrhoea and moderate to severe dehydration with matched controls who had uncomplicated diarrhoea, a number of prognostic factors were assessed. Low body weight, regardless of age, was strongly associated with the risk of dehydration; using 7.0 kg as a cut-off, it had a sensitivity of 75% and a specificity of 68%. Low body weight was superior to more complex anthropometric indices, including weight for age, weight for length or length for age, and also to early signs and symptoms during the episode. By reflecting the effects of both young age and those of malnutrition, low body weight may prove to be a simple indicator for predicting dehydration among children with diarrhoea presenting at a health service.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20248794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial lipopolysaccharide induces diarrhoea in caecectomized mice. 细菌脂多糖诱导阉割小鼠腹泻。
Journal of diarrhoeal diseases research Pub Date : 1996-12-01
F S Robert, J P Rao
{"title":"Bacterial lipopolysaccharide induces diarrhoea in caecectomized mice.","authors":"F S Robert,&nbsp;J P Rao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Castor oil, lipopolysaccharide of Escherichia coli, and endotoxin of Salmonella typhimurium were used for inducing diarrhoea in sham operated or caecectomized mice. Copious diarrhoea was induced by lipopolysaccharide (LPS) in caecectomized mice. Characteristics of diarrhoea induced by castor oil were not different between the two groups. It is concluded that caecectomized mice may be a good model to study lipopolysaccharide-induced diarrhoea.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"14 4","pages":"280-2"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20148705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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