{"title":"Some Characteristics of Transgenic Clones of Mouse R1 Line Embryonic Stem Cells.","authors":"S F Drozd, S A Surkov, M V Glazkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Transgenic clones of mouse embryonic stem cells of the RI line were -received by transfection of plasmid linear vectors. The changes in the transgene structure during its integration into the genome of the target cells were investigated. Displacements were found on the flanks of the integrated transgene. It was found that multicopy tandem structures are formed in head- -tail orientation at the transgene integration. It was noted that the number of copies of the integrated transgenes varies considerably, but the introduction of DNA fragments from the nuclear shells into the flanks of the transgene normalizes the number of its copies.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" 4","pages":"341-347"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36523275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diphyllobothrium dendriticum (Cestoda: Diphyllobothriidae) in the Intestinal Tract of the Herring Gull Larus argentatus: Localization and Trophic Parameters.","authors":"M M Kuklina, V V Kuklin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We studied the activities both of digestive enzymes in the small intestine of the herring gull (Larus argentatus) and a tapeworm Diphyllobothrium dendriticum (Cestoda: Diphyllobothriidae) residing in the intestine. It was found that D. dendriticum infects the medial section of the small intestine of the herring gull. Such localization of D. dendriticum is caused by the maximal activity of proteases and glycosidases and by the high rate of membrane and cavitary digestion in this section. The activity of protease and glycosidase in gulls infected with D. dendriticum is decreased. The activity of proteases in the fractions desorbed from the tegument surface of D. dendriticum is significantly higher than that of glycosidases.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" 4","pages":"389-395"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36520647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Orosensory Food Testing in Fish: Chronology of Behavior.","authors":"E S Mikhailova, A O Kasumyan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The orosensory food testing behavior in fish was studied using the nine-spined stickleback Pungitiuspungitius as an example. The patterns of the chronology of manipulations performed by fish in testing food objects were identified. The existence of two stereotypical patterns of feeding behavior was confirmed, and their new characteristics were obtained. The relationship between the responsiveness to food and the predisposition of fish to feeding was revealed.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" 4","pages":"377-388"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36520646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Neurons with Different Neurotransmitters in Embryonic Neocortical Allografts in the Rat Sciatic Nerve].","authors":"E S Petrova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Different subsets of interneurons in the Wistar rat neocortex and in neocortical transplants developing in a damaged nerve were identified by the following immunohistochemical markers: glutamate decarboxylase (GAD 67) for GABAergic nerve cells, NO-synthase (NOS) for NO-ergic neurons, choline acetyltransferase (ChAT) for cholinergic cells, and tyrosine hydroxylase for catecholaminergic structures. Twenty-eight days after surgery, individual GAD 67-ir, NO-ir, ChAT-ir, and very rarely TH-ir cells were detected in the graft. It was shown that the number of GAD 67-ir neurons per unit area in the grafts was less than in the rat neocortex P20.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"128-35"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34651793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Impact of Radioactive Elements on Microbial Complexes in Cryogenic Soils of Yakutia].","authors":"T I Ivanova, N P Kuz'mina, P I Sobakin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been found that microorganisms in cryogenic soils of Yakutia are resistant to the long-term impact of cesium and thorium. The number of microorganisms in the studied ecological-trophic groups does not depend on the concentrations of radioactive elements. Differences in the number of microorganisms are determined by the physicochemical conditions that are created in different horizons of the soils studied. The long-term impact of radiation (for 36 and 66 years) on microorganisms inhabiting the permafrost soils of Yakutia has developed their adaptive capacity to high concentrations of these radioactive elements.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"146-54"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34651795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V V Barinov, V S Myglan, A N Nazarov, E A Vaganov, A R Agatova, R K Nepop
{"title":"[Extreme Climatic Events in the Altai Republic According to Dendrochronological Data].","authors":"V V Barinov, V S Myglan, A N Nazarov, E A Vaganov, A R Agatova, R K Nepop","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of dating of extreme climatic events by damage to the anatomical structure and missing tree rings of the Siberian larch in the upper forest boundary of the Altai Republic are given. An analysis of the spatial distribution of the revealed dates over seven plots (Kokcy, Chind, Ak-ha, Jelo, Tute, Tara, and Sukor) allowed us to distinguish the extreme events on interregional (1700, 1783, 1788, 1812, 1814, 1884), regional (1724, 1775, 1784, 1835, 1840, 1847, 1850, 1852, 1854, 1869, 1871, 1910, 1917, 1927, 1938, 1958, 1961), and local (1702, 1736, 1751, 1785, 1842, 1843,1874, 1885, 1886, 1919, 2007, and 2009) scales. It was shown that the events of an interregional scale correspond with the dates of major volcanic eruptions (Grimsvotn, Lakagigar, Etna, Awu, Tambora, Soufriere St. Vinsent, Mayon, and Krakatau volcanos) and extreme climatic events, crop failures, lean years, etc., registered in historical sources.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"188-98"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34653500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I V Kovda, E G Morgun, K B Gongalskii, S A Balandin, A I Erokhina
{"title":"[Carbon Isotope Composition in Landscape Components and Its Changes under Different Ecological Conditions].","authors":"I V Kovda, E G Morgun, K B Gongalskii, S A Balandin, A I Erokhina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The composition of stable carbon isotopes in plants, plant litter, leaf litter, and soil organic matter was studied experimentally in the western part of the northern foothills of the Caucasus and mountainsides. It was found that the changes in carbon isotope composition depending on the vertical zonation do not exceed 8 per thousand and depend on the type of C3 plant communities, its presence in biogeocenosis components (living matter, plant litter, soil organic matter), and the degree of moistening of the plot studied.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"216-24"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34653505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[A Complex Morpho-Histological Approach to the In Vitro Study of Morphogenic Structures in a Wheat Anther Culture].","authors":"O A Seldimirova, G E Titova, N N Kruglova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The external morphological and internal histological features of morphogenic structures (embryoids, calli with embryoids, and calli with buds and roots) have been studied in vitro in a wheat anther culture by light microscopy. The results of this study have been compared with data obtained earlier by scanning electron microscopy.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34651796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The Microsculpture of Glochidia of Some Anodontine Bivalves (Unionidae)].","authors":"E M Sayenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Glochidia of three freshwater anodontine bivalves: Kunashiria Starobogatov in Zatrawkin, 1983, Sinanodonta Modell, 1944, from the Far East, and Anodonta Lamarck, 1799, were investigated by scanning electron microscopy. Data on the microsculpture of the outer surface of glochidial valves are given. Among the three genera discussed, the glochidia of Anodonta are the largest, with a loose-looped outer microsculpture and numerous granules. The glochidia of Kunashiria and Sinanodonta differ by the valve height--length proportions and some details of the outer microsculpture: glochidia of Kunashiria have a tight-looped outer sculpture while the glochidia of Sinanodonta have a loose-net outer sculpture.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"162-70"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34653497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A F Sabrekov, I V Filippov, I E Terentieva, M V Glagolev, D V Il'yasov, B A Smolentsev, Sh Sh Maksyutov
{"title":"[The Spatial Variability of Methane Emission from Grass-Moss Fens of the Subtaiga and Forest-Steppe of Western Siberia].","authors":"A F Sabrekov, I V Filippov, I E Terentieva, M V Glagolev, D V Il'yasov, B A Smolentsev, Sh Sh Maksyutov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Emission of methane from the grass-moss fens of the Western Siberia subtaiga was studied using a static chamber method. It was established that a median half of the interquartile range of the specific flow of CH4 in the studied wetland ecosystems constituted 4.9 ± 2.9 mg of CH4/(m2 x h). It was shown that such a high spatial variability of emission is caused mainly by the difference in the level of fen waters. It was found that, in these observations, a higher level of the water stand correlates with lower emission values. The causes of this phenomenon are discussed, and recommendations for conducting field studies for estimating the regional flow are given.</p>","PeriodicalId":77187,"journal":{"name":"Izvestiia Akademii nauk. Seriia biologicheskaia","volume":" ","pages":"199-206"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34653503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}