International journal of clinical & laboratory research最新文献_第9页

Absence of antibodies to tissue-type plasminogen activator in patients with previous deep vein thrombosis. 既往深静脉血栓患者缺乏组织型纤溶酶原激活剂抗体。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050020

M Cugno, CattaneoM, G Bisiani, M Cabibbe, I Martinelli

引用次数: 3

Measurement of reactive oxygen species by chemiluminescence in diet-induced atherosclerosis: protective roles of vitamin E and probucol on different radical species. 化学发光法测定饮食诱导动脉粥样硬化中的活性氧:维生素E和普罗布考对不同自由基的保护作用。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050031

G Haklar, O Sirikçi, N K Ozer, A S Yalçin

{"title":"Measurement of reactive oxygen species by chemiluminescence in diet-induced atherosclerosis: protective roles of vitamin E and probucol on different radical species.","authors":"G Haklar, O Sirikçi, N K Ozer, A S Yalçin","doi":"10.1007/s005990050031","DOIUrl":"https://doi.org/10.1007/s005990050031","url":null,"abstract":"We have investigated the effects of a high-cholesterol diet on the production of different reactive oxygen species in rabbit aortic rings and evaluated the protective effects of vitamin E and probucol in preventing peroxidative changes. Twenty-five male albino rabbits were divided into five groups. Control rabbits were fed a vitamin E-poor rabbit chow. Rabbits in the second group were given a vitamin E-poor diet supplemented with 2% cholesterol. Other groups received either 50 mg/kg vitamin E, 1% probucol, or both, in addition to 2% cholesterol for 4 weeks. Reactive oxygen species formation in aortic rings was measured by enhanced chemiluminescence using luminol and lucigenin. (The results were given as cpm/mg wet weight.) Further differentiation of radical species involved in luminol-enhanced chemiluminescence was performed using sodium azide and L-nitroarginine, a selective inhibitor of nitric oxide production. Our results indicated that cholesterol feeding increased lucigenin and luminol chemiluminescence, where the contribution of free radicals inhibited by sodium azide (radicals originating from endothelial cells or from phagocytes) were 53% and peroxynitrite 24%. Both vitamin E and probucol were effective as scavengers of free radicals, but the effect of vitamin E was more pronounced. In conclusion, the present study demonstrated excessive generation of reactive oxygen species within the atherosclerotic vessel. Peroxidative changes could be prevented by vitamin E and probucol treatment, but vitamin E seemed to be more efficient.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 2","pages":"122-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20606145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

The effectiveness and tolerability of epoetin alfa in patients with multiple myeloma refractory to chemotherapy. 促生成素在化疗难治性多发性骨髓瘤患者中的疗效和耐受性。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050032

F Dammacco, F Silvestris, G L Castoldi, B Grassi, C Bernasconi, G Nadali, G Perona, A De Laurenzi, U Torelli, E Ascari, P L Rossi Ferrini, F Caligaris-Cappio, A Pileri, L Resegotti

{"title":"The effectiveness and tolerability of epoetin alfa in patients with multiple myeloma refractory to chemotherapy.","authors":"F Dammacco, F Silvestris, G L Castoldi, B Grassi, C Bernasconi, G Nadali, G Perona, A De Laurenzi, U Torelli, E Ascari, P L Rossi Ferrini, F Caligaris-Cappio, A Pileri, L Resegotti","doi":"10.1007/s005990050032","DOIUrl":"https://doi.org/10.1007/s005990050032","url":null,"abstract":"Anemia is a frequent complication of multiple myeloma, becoming chronic in patients who are resistant to chemotherapy. This randomized, parallel, controlled multicenter study (71 patients receiving concomitant chemotherapy) evaluated the efficacy and safety of epoetin alfa in improving anemia and eliminating the need for transfusions in multiple myeloma patients refractory to conventional first- or second-line chemotherapy. Forty patients were treated with subcutaneous epoetin alfa (150 IU/kg per dose, increasing to 300 IU/kg per dose, every 3 weeks) for 6 months, and 31 entered a control group. The epoetin alfa group had a significantly (P < or = 0.001) greater percentage of patients (75% vs. 21%) with increases in hemoglobin levels and/or reduced transfusion requirements. In 44 non pre-transfused patients (20 controls, 24 in the epoetin alfa group), the mean increase in hemoglobin was significantly (P < or = 0.0001) greater in the epoetin alfa group (+2.1 vs. -0.2 g/dl). Increases in hematocrit and red blood cells were also significantly (P < or = 0.0001) greater in epoetin alfa-treated patients, with corresponding reductions in transfusion requirement. In the 27 pre-transfused patients (11 controls, 16 in the epoetin alfa group), there was a trend towards reduced transfusional need in epoetin alfa-treated patients. Thus, in patients with multiple myeloma refractory to chemotherapy epoetin alfa is a well-tolerated treatment which improves anemia in non pre-transfused patients and appears to reduce transfusion need in those previously transfused.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 2","pages":"127-34"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20606639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Influence of smoking habits on Down's syndrome risk evaluation at mid-trimester through biochemical screening. 通过生化筛查，吸烟习惯对妊娠中期唐氏综合征风险评估的影响

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050040

M Perona, G Mancini, D Dall'Amico, V Guaraldo, A Carbonara

{"title":"Influence of smoking habits on Down's syndrome risk evaluation at mid-trimester through biochemical screening.","authors":"M Perona, G Mancini, D Dall'Amico, V Guaraldo, A Carbonara","doi":"10.1007/s005990050040","DOIUrl":"https://doi.org/10.1007/s005990050040","url":null,"abstract":"Expectant mothers who smoke have higher levels of maternal serum alpha-fetoprotein and lower levels of unconjugated estriol and total human chorionic gonadotrophin than non-smoking mothers. This significantly affects performance of screening for Down's syndrome. This study includes 22,169 pregnant women: 18,876 non-smokers, 2,660 smoking < or = 10 cigarettes/day, and 633 smoking > 10 cigarettes/day. Mean maternal age (32.6 years), maternal weight (60.5 kg), and gestational age (114.7 days) were similar or only slightly different between the three groups. To verify the effects of smoking on screening, we studied retrospectively 130 sequential Down's syndrome cases (47 from the screening program, 83 from the prenatal diagnosis program). The proportion of smokers in the Down's syndrome and unaffected pregnancies was similar, whilst the false-positive rate and detection rate, based on fetal outcome, differed: false-positive rates were 5.63% in smokers and 9.42% in non-smokers, and detection rate 55.6% in smokers and 83.0% in non-smokers. Since the prevalence of Down's syndrome pregnancies was the same at mid-trimester in smokers and non-smokers and the proportion of smokers was not related to maternal age, we propose an adjustment of the Down's syndrome risk evaluation algorithm according to smoking habits.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 3","pages":"179-82"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20712812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Effect of HMG-CoA reductase inhibitors on plasma polyunsaturated fatty acid concentrations in patients with hyperlipidemia. HMG-CoA还原酶抑制剂对高脂血症患者血浆多不饱和脂肪酸浓度的影响

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050043

N Nakamura, T Hamazaki, H Jokaji, S Minami, M Kobayashi

引用次数: 46

The effect of bilirubin on lipid peroxidation and antioxidant enzymes in cumene hydroperoxide-treated erythrocytes. 胆红素对异丙苯氢过氧化物处理红细胞脂质过氧化和抗氧化酶的影响。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/BF02874114

A Yeşilkaya, A Yeğin, S Ozdem, T A Aksu

引用次数: 12

Effect of low-dose heparin on fibrinogen levels in patients with chronic ischemic heart disease. 低剂量肝素对慢性缺血性心脏病患者纤维蛋白原水平的影响。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050038

D Prisco, R Paniccia, B Bandinelli, A M Gori, M Attanasio, B Giusti, M Comeglio, R Abbate, G F Gensini, G G Serneri

{"title":"Effect of low-dose heparin on fibrinogen levels in patients with chronic ischemic heart disease.","authors":"D Prisco, R Paniccia, B Bandinelli, A M Gori, M Attanasio, B Giusti, M Comeglio, R Abbate, G F Gensini, G G Serneri","doi":"10.1007/s005990050038","DOIUrl":"https://doi.org/10.1007/s005990050038","url":null,"abstract":"Several prospective studies have demonstrated that high plasma fibrinogen levels are associated with an increased risk of ischemic heart disease. Since in most patients an increased thrombin generation has been reported, we investigated whether the control of thrombin generation could affect plasma fibrinogen levels. Forty male outpatients (20 asymptomatic with previous myocardial infarction and 20 with stable effort angina) were enrolled in a randomized medium-term (6 months) cross-over study. Clottable fibrinogen, according to Clauss, prothrombin fragment 1 + 2, thrombin-antithrombin complex, and fibrinopeptide A were evaluated in relation to treatment with low-dose heparin. After a 15-day wash-out period, during which patients had been treated only with nitrates if needed, patients were allocated to two sequential periods of treatment with standard heparin (12,500 U, subcutaneously daily) plus antianginal treatment or antianginal treatment alone, separated by a second 15-day wash-out period. At the end of the treatment period with low-dose heparin significant decreases in the plasma fibrinogen (2.5 +/- 0.6 g/l vs. 3.3 +/- 0.5 g/l, P < 0.001), prothrombin fragment 1 + 2 (1.4 +/- 0.5 nmol/l vs. 1.9 +/- 0.7 nmol/l, P < 0.001), thrombinantithrombin (4.5 +/- 2.4 ng/ml vs. 9.7 +/- 3.6 ng/ml, P < 0.001), and fibrinopeptide A (2.1 +/- 1.1 ng/ml vs. 3.5 +/- 2.1 ng/ml, P < 0.001) were observed compared with the period without heparin. The present results indicate that low-dose heparin can effectively control the increased abnormal thrombin generation and elevated fibrinogen levels in patients with ischemic heart disease, possibly decreasing the risk of cardiovascular death.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 3","pages":"170-3"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20712810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

The effect on transcription efficiency of the apolipoprotein AI gene of DNA variants at the 5' untranslated region. 5'非翻译区DNA变异对载脂蛋白AI基因转录效率的影响。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050051

X L Wang, R B Badenhop, A S Sim, D E Wilcken

{"title":"The effect on transcription efficiency of the apolipoprotein AI gene of DNA variants at the 5' untranslated region.","authors":"X L Wang, R B Badenhop, A S Sim, D E Wilcken","doi":"10.1007/s005990050051","DOIUrl":"https://doi.org/10.1007/s005990050051","url":null,"abstract":"Elevated circulating levels of high-density lipoprotein and apolipoprotein AI are associated with reduced coronary artery disease risk. We have shown that a C to T substitution at +83 bp and a G to A substitution at -75 bp of the apolipoprotein AI gene are both related to increased high-density lipoprotein levels in a healthy population but not in a coronary population, among whom the same mutations are associated with increased disease severity. In the present study, we explored the effects of these base changes on transcriptional efficiency in vitro. We directionally cloned (using polymerase chain reaction) the 5' region of the apolipoprotein AI gene (-281 to +330 bp) with GC, GT, and AC haplotypes into a pGL3-luciferase reporter gene basic vector, and transfected the constructed vectors into HepG2 cells. The cells carrying the T allele at the +83 bp site (GT 112.3 +/- 12.4) had the same transcriptional efficiency as those bearing the C allele (GC 126.3 +/- 9.6). However, for cells with the A allele at -75 bp there was a twofold decrease in transcription (AC 63.1 +/- 9.3) accompanied by similar changes in Luc+ mRNA levels; this reduced transcription was only present if the apolipoprotein AI leader sequence was included in the insert. While the findings are inconsistent with the T or A allele being associated with higher high-density lipoprotein levels, they are consistent with the finding that the alleles are associated with an increased coronary artery disease risk, and demonstrate that the 5' leader region of the apolipoprotein AI gene participates in regulating apolipoprotein AI transcription. They also suggest that other regions of the apolipoprotein AI gene may have an active role in such regulation, and that environmental effects may influence allele-specific expression.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 4","pages":"235-41"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20786116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Influence of iron overload on manganese, zinc, and copper concentration in rat tissues in vivo: study of liver, spleen, and brain. 铁超载对体内大鼠组织中锰、锌和铜浓度的影响:肝、脾和脑的研究。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050041

D V Vayenas, M Repanti, A Vassilopoulos, D A Papanastasiou

{"title":"Influence of iron overload on manganese, zinc, and copper concentration in rat tissues in vivo: study of liver, spleen, and brain.","authors":"D V Vayenas, M Repanti, A Vassilopoulos, D A Papanastasiou","doi":"10.1007/s005990050041","DOIUrl":"https://doi.org/10.1007/s005990050041","url":null,"abstract":"Although hemochromatosis and pathological situations due to chronic iron overload have been extensively described, there is little information about the influence of iron on other trace elements in the cell. The aim of this study was to investigate changes in the concentration of zinc, manganese, and copper in the liver, spleen, and brain of rats after iron overload. Iron overload in Wistar rats was achieved by iron-supplemented diet or by intraperitoneal or intravenous injection of polymaltose iron. Iron, zinc, manganese, and copper were determined by atomic absorption spectrophotometry. Iron overload in rats, regardless of the route of its application, resulted in an increase not only of iron but also of zinc and manganese in the liver and the spleen, whereas the content of these metals in the brain did not change. The copper content of the liver, spleen, and brain remained the same after iron overload. The increase of zinc and manganese in the liver and spleen following iron overload was probably a result not only of increased intestinal absorption but also of increased uptake from the cell. This is also supported by the fact that no increase in the zinc and manganese concentrations occurred in the brain since, despite iron overload, the iron content remained constant.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 3","pages":"183-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20712813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Early prognostic biochemical indicators of fulminant hepatic failure. 暴发性肝衰竭的早期预后生化指标。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050044

P Carraro, D Burighel, G De Silvestro, M Gaiotto, M Plebani

引用次数: 11