International journal of clinical & laboratory research最新文献_第10页

Treatment of von Willebrand disease. 血管性血友病的治疗

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050047

P M Mannucci

引用次数: 4

Evaluation of biotinylated cells as a source of antigens for characterization of their molecular profile. 生物素化细胞作为抗原来源的评价，以表征其分子特征。

International journal of clinical & laboratory research Pub Date : 1998-01-01 DOI: 10.1007/s005990050053

F Perosa, G Luccarelli, M Neri, V De Pinto, S Ferrone, F Dammacco

{"title":"Evaluation of biotinylated cells as a source of antigens for characterization of their molecular profile.","authors":"F Perosa, G Luccarelli, M Neri, V De Pinto, S Ferrone, F Dammacco","doi":"10.1007/s005990050053","DOIUrl":"https://doi.org/10.1007/s005990050053","url":null,"abstract":"Biotinylated lymphoid cells have been suggested as a useful source of antigen for the immunochemical characterization of their molecular profile. Labelling with biotin eliminates the problems associated with the use of radioactivity. However, this method has not been widely used. This reflects: (1) difficulties in optimizing the signal/background ratio because of the lack of a simple method to quantify biotinylated proteins in a cell lysate, (2) the loss of reactivity with monoclonal antibody of antigen following biotinylation, because of steric hindrance, and (3) the lack of information about the utility of other biotinylated cells as an antigen source. To overcome these limitations, we developed an ELISA to quantify biotinylated proteins in cell lysates and optimized the signal/background ratio. The validity of this approach was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of a number of cell surface antigens immunoprecipitated from lymphoid cells by an optimal amount of monoclonal antibody. Furthermore, we showed that biotinylated melanoma cells are a useful source of antigen for immunoprecipitation experiments and that ligation of biotin to antigen does not affect reactivity with monoclonal antibody. Lastly, biotinylated antigens in cell lysates stored at -80 degrees C for 6 months maintained their reactivity with monoclonal antibodies. Biotinylated cells thus represent a useful source of antigen for characterizing the immunochemical profile and analyzing the specificity of antibodies with immunochemical methods.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"28 4","pages":"246-51"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20786118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

IgG M-components in active myeloma patients induce a down-regulation of natural killer cell activity. 活动性骨髓瘤患者IgG m成分诱导自然杀伤细胞活性下调。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02827242

M A Frassanito, F Silvestris, P Cafforio, N Silvestris, F Dammacco

{"title":"IgG M-components in active myeloma patients induce a down-regulation of natural killer cell activity.","authors":"M A Frassanito, F Silvestris, P Cafforio, N Silvestris, F Dammacco","doi":"10.1007/BF02827242","DOIUrl":"https://doi.org/10.1007/BF02827242","url":null,"abstract":"Natural killer cell activity and related cell surface markers of peripheral blood lymphocytes were studied in 73 patients with multiple myeloma, 25 with monoclonal gammopathy of undetermined significance and 20 normal controls. Natural killer cell number was significantly higher in both multiple myeloma and monoclonal gammopathy patients than in controls, whereas the natural killer activity of multiple myeloma patients was inversely related to their disease status. Incubation of peripheral blood lymphocytes or natural killer cells with IgG myeloma proteins purified from several patients induced a down-modulation of basic natural killer activity. This inhibitory effect of monoclonal IgG was dose dependent and significantly stronger in patients with active (at diagnosis and at relapse) than stable multiple myeloma or in normal controls. Addition of exogenous recombinant interleukin-2 restored natural killer cell activity against K562 target cells, indicating that natural killer cells were able to recover their functions. However, recombinant interleukin-2-stimulated natural killer cells were responsive to down-modulation of monoclonal IgG. These data suggest that impaired natural killer cell function in active multiple myeloma is caused by the inhibitory effect of M-component.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"27 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02827242","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20091116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Increased thrombin generation in patients with chronic renal failure. 慢性肾衰竭患者凝血酶生成增加。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02827247

A Sagripanti, V Cozza, U Baicchi, M Camici, A Cupisti, G Barsotti

{"title":"Increased thrombin generation in patients with chronic renal failure.","authors":"A Sagripanti, V Cozza, U Baicchi, M Camici, A Cupisti, G Barsotti","doi":"10.1007/BF02827247","DOIUrl":"https://doi.org/10.1007/BF02827247","url":null,"abstract":"The plasma concentration of prothrombin fragment 1 + 2 (F1 + 2) is considered a very sensitive parameter for specific detection of latent hypercoagulability. To evaluate the degree of hypercoagulation associated with chronic uremia, we measured F1 + 2 by ELISA in the plasma of 51 patients with severe or end-stage chronic renal failure (35 males, 16 females, aged 22-81 years): 24 on dietary treatment 15 on combined dietary and once a week hemodialysis, and 12 on regular maintenance hemodialysis; 33 healthy subjects served as a control group. Plasma F1 + 2 showed a significant elevation in the group on dietary treatment; it was further increased in the group on once a week hemodialysis, and even more markedly increased in the group on maintenance hemodialysis. In patients on dietary treatment a positive correlation was found between plasma F1 + 2 and serum creatinine. In patients on maintenance hemodialysis, no increase in the F1 + 2 plasma level was found during the course of a single hemodialysis session. Low molecular weight heparin, administered to 7 patients on dietary treatment, caused a marked drop in the F1 + 2 plasma level, providing evidence that the elevation in F1 + 2 indicates an accelerated in vivo thrombin generation rather than impaired renal catabolism. The enhanced coagulation activation appears to be related to the reduction of residual renal function, i.e., to the severity of renal failure, and may contribute to the increased risk of vascular events in uremic patients.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"27 1","pages":"72-5"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02827247","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20091121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Successful treatment of common variable immunodeficiency and related disorders with cimetidine and zinc sulfate. 西咪替丁和硫酸锌成功治疗常见的变异性免疫缺陷及相关疾病。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02827249

S Della Bella, M Vanoli, S Bazzi, R Scorza

引用次数: 2

Tumor antigens recognized by T lymphocytes. T淋巴细胞识别的肿瘤抗原。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02912440

B J Van den Eynde, T Boon

引用次数: 312

Gamma delta T-cells in human cutaneous immunology. γ δ t细胞在人体皮肤免疫中的作用。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02912452

M Alaibac, J Morris, A C Chu

引用次数: 11

Peripheral neutrophils after allergic asthmatic reactions. 过敏性哮喘反应后的外周中性粒细胞。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02912455

B Asman, V Strand, G Bylin, K Bergström

{"title":"Peripheral neutrophils after allergic asthmatic reactions.","authors":"B Asman, V Strand, G Bylin, K Bergström","doi":"10.1007/BF02912455","DOIUrl":"https://doi.org/10.1007/BF02912455","url":null,"abstract":"The response of peripheral neutrophils was studied in 16 patients with allergic asthma after challenge with birch/grass pollen allergen, in order to identify inflammatory markers associated with only the early asthmatic reaction and those associated with both early and late asthmatic reactions. The allergen challenge proceeded until the patients had an early asthmatic reaction with 100% increase in specific airway resistance. Bronchoconstriction after allergen challenge was monitored hourly over 9 h and finally after 18 h, by measurement of the forced expiratory volume in 1 s. Seven patients had a late reaction, defined as a decrease in forced expiratory volume in 1 s of more than 15%. Blood samples were taken before and 18 h after challenge. After allergen challenge (18 h) the blood concentration of neutrophils in patients with a late asthmatic reaction was 1.4 times higher than before challenge and there was a tendency for increased Fc gamma receptor-mediated chemiluminescence. Lewis X-antigen (CD 15), which is associated with endothelial adhesion and extravasation, significantly decreased at the same time. Neutrophils were incubated with the tetrapeptide arginine-glycine-aspartate-serine before and 18 h after allergen challenge. Both patient groups showed an increased Fc gamma receptor-mediated chemiluminescence and a decreased Fc gamma receptor membrane expression following allergen challenge, suggesting a preactivation. In conclusion, patients with a dual asthmatic reaction show a sustained primed inflammatory response and primed neutrophils compared with patients with only an early reaction when measured after the decline of clinical symptoms provoked by allergen challenge.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"27 3","pages":"185-8"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02912455","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20283423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Hepatitis viruses: genetic variants and clinical significance. 肝炎病毒:基因变异及其临床意义。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02912461

H E Blum

引用次数: 12

T-cell recognition of melanoma antigens and its therapeutic applications. 黑色素瘤抗原的t细胞识别及其治疗应用。

International journal of clinical & laboratory research Pub Date : 1997-01-01 DOI: 10.1007/BF02912443

C Castelli, L Rivoltini, A Mazzocchi, G Parmiani

{"title":"T-cell recognition of melanoma antigens and its therapeutic applications.","authors":"C Castelli, L Rivoltini, A Mazzocchi, G Parmiani","doi":"10.1007/BF02912443","DOIUrl":"https://doi.org/10.1007/BF02912443","url":null,"abstract":"During the last few years, tumor immunology has gained impetus due to the molecular definition of T-cell-recognized antigens and the mechanisms of such recognition, antigen processing, and presentation. To date, the majority of the identified melanoma antigens are shared among different melanomas and some are also expressed in tumors of different histology. However, unique antigens expressed solely by the melanoma autologous to the T-cell used for their characterization were also found. The identification of the immunogenic peptides, the minimal target entity required for T-cell recognition, has provided novel reagents for the development of peptide-based immunotherapy. These findings, together with the understanding of requirements for T-lymphocyte recognition and activation, allow the design of new therapeutic protocols. In addition, the large body of data now available on the fine mechanism of antigen processing and presentation have revealed not only the role of the MHC molecules but also that of other intracellular proteins, such as transporter associated with antigen processing-1 and -2 and proteosome-related molecules. These findings suggest that, in order to select patients eligible for vaccination, the expression of the MHC allele involved in T-cell recognition, the profile of tumor antigens, and the status of the antigen-processing system should be carefully evaluated in tumors cells of prospective patients. In this review, some of the basic concepts of immune recognition and the current view of melanoma tumor antigens recognized by T-lymphocytes will be discussed along with the potential application of these findings in designing new therapeutic strategies.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"27 2","pages":"103-10"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02912443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20208747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11