International journal of clinical & laboratory research最新文献_第3页

Joint effects of HMG-CoA reductase inhibitors and eicosapentaenoic acids on serum lipid profile and plasma fatty acid concentrations in patients with hyperlipidemia. HMG-CoA还原酶抑制剂和二十碳五烯酸对高脂血症患者血脂和血浆脂肪酸浓度的联合影响

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050057

N Nakamura, T Hamazaki, M Ohta, K Okuda, M Urakaze, S Sawazaki, K Yamazaki, A Satoh, R Temaru, Y Ishikura, M Takata, M Kishida, M Kobayashi

{"title":"Joint effects of HMG-CoA reductase inhibitors and eicosapentaenoic acids on serum lipid profile and plasma fatty acid concentrations in patients with hyperlipidemia.","authors":"N Nakamura, T Hamazaki, M Ohta, K Okuda, M Urakaze, S Sawazaki, K Yamazaki, A Satoh, R Temaru, Y Ishikura, M Takata, M Kishida, M Kobayashi","doi":"10.1007/s005990050057","DOIUrl":"https://doi.org/10.1007/s005990050057","url":null,"abstract":"HMG-CoA reductase inhibitors reduce serum total cholesterol concentrations and the risk of coronary heart disease in patients with hypercholesterolemia. Recently, it has been reported that patients with combined hyperlipidemia are also at risk of coronary heart disease. However, HMG-CoA reductase inhibitor therapy alone does not sufficiently reduce serum triglyceride concentrations. Epidemiological and clinical evidence has shown that fish oil can lower plasma lipid levels, especially triglycerides. Consequently, we investigated the effects of the combination of HMG-CoA reductase inhibitors and eicosapentaenoic acid, a major component of fish oil, on hyperlipidemia. We administered 900-1,800 mg/day of the ethyl ester of eicosapentaenoic acid to patients with hyperlipidemia who had been treated with HMG-CoA reductase inhibitors for 30 +/- 6 months (means +/- SE). Serum total cholesterol and triglyceride concentrations were significantly decreased 3 months after the administration of eicosapentaenoic acid (from 5.63 +/- 0.23 mmol/l to 5.02 +/- 0.20 mmol/l, P < 0.05; from 2.07 +/- 0.41 mmol/l to 1.08 +/- 0.17 mmol/l, P < 0.01, respectively). Serum high-density lipoprotein-cholesterol concentrations were significantly increased after the treatment (from 1.23 +/- 0.12 mmol/l to 1.34 +/- 0.13 mmol/l, P < 0.05). Plasma eicosapentaenoic acid concentrations and the ratio to arachidonic acid in plasma were also significantly increased 3 months after the treatment (from 101.9 +/- 8.1 mg/l to 181.8 +/- 23.9 mg/l, P < 0.001; from 0.640 +/- 0.075 to 1.211 +/- 0.170, P < 0.001, respectively). These results suggested that the combination therapy of HMG-CoA reductase inhibitors and eicosapentaenoic acid was effective for patients with hyperlipidemia.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 1","pages":"22-5"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21225095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Serum calcitriol and dietary protein intake in idiopathic calcium stone patients. 特发性钙结石患者血清骨化三醇和膳食蛋白质的摄入。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050069

A Cupisti, E Morelli, M Meola, C Lenti, M Barsotti, D Palmieri, S Sposini, G Barsotti

{"title":"Serum calcitriol and dietary protein intake in idiopathic calcium stone patients.","authors":"A Cupisti, E Morelli, M Meola, C Lenti, M Barsotti, D Palmieri, S Sposini, G Barsotti","doi":"10.1007/s005990050069","DOIUrl":"https://doi.org/10.1007/s005990050069","url":null,"abstract":"In kidney stone patients, high protein intake and calcitriol overproduction are factors leading to hypercalciuria, but there are conflicting reports on the effects of dietary protein on calcitriol production. To investigate the relationships between serum calcitriol, dietary protein intake, and urinary calcium excretion, 33 male idiopathic calcium stone formers (aged 20-60 years), with normal renal function and on unrestricted diet, have been studied. Dietary protein intake was estimated by the protein catabolic rate determination. Abnormally elevated calcitriol levels were found in 16 patients (48.5%) who had similar levels of serum intact parathyroid hormone and phosphate, creatinine clearance, and calcium and phosphate urinary excretion, but lower protein catabolic rate (82+/-16 vs. 97+/-20 g/day, P<0.05) than the patients with normal calcitriol levels. The calcitriol to intact parathyroid hormone ratio was higher in hypercalciuric than in normocalciuric patients (2.4+/-1.1 vs. 1.6+/-0.8, P<0.05). Calcitriol was positively correlated with plasma calcium (r=0.41, P<0.01) and inversely with protein catabolic rate (r=-0.42, P<0.01). Protein catabolic rate was positively correlated with creatinine clearance (r=0.69, P<0.001) and urinary phosphate excretion (r=0.72, P<0.001). No relationship was observed between calcitriol and creatinine clearance. These results confirm the calcitriol overproduction in calcium stone disease and that the high calcitriol to intact parathyroid hormone ratio is the main feature associated with hypercalciuria. Calcitriol serum levels appear to be unrelated to creatinine clearance, whereas there is an inverse relationship with protein catabolic rate. This suggests that low rather than high dietary protein intake may favor the increase of calcitriol synthesis in male calcium stone formers with normal renal function.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 2","pages":"85-8"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21301019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Measurement of hepatocyte growth factor in serum and plasma. 血清和血浆中肝细胞生长因子的测定。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050073

M Sakon, Y Kita, Y Takeda, N Higaki, H Ohzato, K Umeshita, Y Hasuike, M Gotoh, M Monden

{"title":"Measurement of hepatocyte growth factor in serum and plasma.","authors":"M Sakon, Y Kita, Y Takeda, N Higaki, H Ohzato, K Umeshita, Y Hasuike, M Gotoh, M Monden","doi":"10.1007/s005990050073","DOIUrl":"https://doi.org/10.1007/s005990050073","url":null,"abstract":"To investigate whether serum or plasma should be used for the measurement of blood hepatocyte growth factor, the levels were compared in 28 normal subjects and 30 patients who had undergone surgery. The serum level was significantly higher than the plasma level. The serum and plasma hepatocyte growth factor levels differed markedly depending on the subjects, although overall there was a significant correlation between levels (r=0.862, P=0.0001). In serum obtained by the clotting of platelet- or leukocyte-containing plasma with thrombin, hepatocyte growth factor increased in proportion to the number of leukocytes. The difference between serum and plasma hepatocyte growth factor levels also correlated with the number of leukocytes in the patients (r=0.642, P=0.0004). Such a correlation was not observed for platelets. These findings suggest that the serum hepatocyte growth factor level does not strictly reflect the in vivo blood level, due to the release from leukocytes during sample preparation (i.e., blood clotting ) and that plasma is more suitable for assay of blood hepatocyte growth factor.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 3","pages":"110-3"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21450498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

The molecular basis of von Willebrand disease. 血管性血友病的分子基础。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050054

K L Mohlke, W C Nichols, D Ginsburg

引用次数: 24

An update on the role of free radicals and antioxidant defense in human disease. 自由基和抗氧化防御在人类疾病中的作用的最新进展。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050063

G Vendemiale, I Grattagliano, E Altomare

引用次数: 275

Composition of the sugar moiety of Tamm-Horsfall protein in patients with urinary diseases. 泌尿系统疾病患者Tamm-Horsfall蛋白糖段的组成。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050066

T Olczak, M Olczak, A Kubicz, J Duława, F Kokot

{"title":"Composition of the sugar moiety of Tamm-Horsfall protein in patients with urinary diseases.","authors":"T Olczak, M Olczak, A Kubicz, J Duława, F Kokot","doi":"10.1007/s005990050066","DOIUrl":"https://doi.org/10.1007/s005990050066","url":null,"abstract":"The sugar moiety of Tamm-Horsfall protein (THP) is altered by pathological conditions. The aim of this study was to investigate the composition of THP glycans in urinary diseases. THP was isolated from the urine of patients with urinary tract infection (group A), glomerulonephritis or interstitial nephritis (group B), and Bartter's syndrome (BS) (group C). Monosaccharides, N-glycan profile, THP reactivity with specific lectins and some other proteins were analyzed. THP of patients from groups A, B, and C showed lower amounts of N-acetylgalactosamine (P<0.05, P<0.005, and P<0.05, respectively) than controls; this was reflected in lower reactivity with Phaseolus vulgaris lectin (P<0.005, P<0.05, and P<0.005). Reduced amounts of N-acetylglucosamine were noticed in groups A (P<0. 05) and B (P<0.05). In group A lower amounts of galactose and alpha2, 6-linked sialic acid, as determined by reactivity with Datura stramonium lectin (P<0.005) and Sambucus nigra lectin (P<0.005), were observed. In patients with BS there was a shift from tetrasialylated glycans towards less-sialylated chains. We found also that THP of all patients binds more strongly to IgG(1) (P<0.005, for all patient groups). Our results indicate that the urinary diseases examined affect the THP sugar moiety and the binding of THP to IgG(1).","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 2","pages":"68-74"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21301140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Selective upregulation of MHC class I expression in metastatic colonies derived from tumor clones of a murine fibrosarcoma. 小鼠纤维肉瘤肿瘤克隆的转移菌落中MHC I类表达的选择性上调。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050085

S Pedrinaci, I Algarra, A Garcia Lora, J J Gaforio, M Perez, F Garrido

{"title":"Selective upregulation of MHC class I expression in metastatic colonies derived from tumor clones of a murine fibrosarcoma.","authors":"S Pedrinaci, I Algarra, A Garcia Lora, J J Gaforio, M Perez, F Garrido","doi":"10.1007/s005990050085","DOIUrl":"https://doi.org/10.1007/s005990050085","url":null,"abstract":"Eighteen metastatic nodes derived from the wild-type (GR9) and from 4 different clones (G2, D8, B10, and B9) obtained from a fibrosarcoma were analyzed for H-2 class I and II expression, as well as for adhesion molecules (CD44, CDIIb, CD18, CD49, and CD54). When metastatic nodes were cultured, typed for H-2 antigens, and compared with the H-2 expression of the inducing tumor cell, H-2 Kd and Dd class I expression was greater in most nodes analyzed. In contrast, the Ld molecule remained negative, or showed a minor increase. Class II expression was negative in the wild-type and the tumor clones, and remained so in the metastatic colonies. Analysis of the adhesion molecules revealed no differences between the inducing tumor cells and the metastatic nodes. The only molecule expressed was CD44, which was present in all cells studied and was also inducible by interferon-gamma. The increase in H-2K and H-2D expression was associated with resistance to natural killer cytotoxicity, as observed in the G2 tumor clone and some autologous metastases, such as B9MP2, G2MK2, and G2MLI. In three independent clones of this tumor system (D8, BIOMP6, and B9MP6) we found that tumor cells treated with interferon-gamma had the same altered phenotype, i.e., a selective lack of response of the Ld molecule to induction. These findings add a cautionary note to the well-established idea that tumor cells may lose all class I antigens during tumor progression, and suggest that sometimes this may not be the case. The selective downregulation of Ld and upregulation of Kd and Dd class I expression may give some tumor cells means of escaping both cytotoxic lymphocyte and natural killer immune surveillance.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 4","pages":"166-73"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Protein-bound polysaccharide (PSK) induces cytotoxic activity in the NKL human natural killer cell line. 蛋白结合多糖(PSK)诱导NKL人自然杀伤细胞系的细胞毒活性。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050079

S Pedrinaci, I Algarra, F Garrido

{"title":"Protein-bound polysaccharide (PSK) induces cytotoxic activity in the NKL human natural killer cell line.","authors":"S Pedrinaci, I Algarra, F Garrido","doi":"10.1007/s005990050079","DOIUrl":"https://doi.org/10.1007/s005990050079","url":null,"abstract":"We studied the effect of protein-bound polysaccharide PSK on the activation of the human natural killer cell line NKL. We observed an increased natural killer cytotoxic activity against different tumor cells (K562, Daudi, and U937) when a standard 2- to 3-h 51chromium release assay was performed. The results parallel those obtained after treatment of the NKL cell line with interleukin-2. The highest cytotoxic activity was reached at a concentration of 100 microg/ml of PSK. This natural killer activation was inhibited when the PSK dose was 1,000 microg/ml. None of the cell surface markers that were analyzed by fluorescence-activated cell sorting showed variations after PSK or interleukin-2 treatment of NKL cells. These markers included CD2, CD11b, CD11c, CD18, CD16, CD54, CD56, CD98, CD25, CD122, HLA class I, HLA class II, CD94, ILT2, p58.1, p70, and NKp46. One of these markers (NKp46) is a major triggering receptor reported to be involved in the natural cytotoxicity of fresh or cultured human natural killer cells. In our study, another triggering receptor must be implicated in PSK-induced natural killer lysis. Our data suggest that PSK is an important biological response modifier of natural killer cells in vitro and may prove to be useful for the study of human natural killer cell biology.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 4","pages":"135-40"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

The pre-procedural platelet state predicts clinical recurrence after coronary angioplasty. 术前血小板状态预测冠状动脉成形术后临床复发。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050081

M Capanni, D Prisco, E Antonucci, L Chiarugi, V Boddi, R Abbate, C Giglioli, R P Dabizzi, M Margheri, I Simonetti, G F Gensini

{"title":"The pre-procedural platelet state predicts clinical recurrence after coronary angioplasty.","authors":"M Capanni, D Prisco, E Antonucci, L Chiarugi, V Boddi, R Abbate, C Giglioli, R P Dabizzi, M Margheri, I Simonetti, G F Gensini","doi":"10.1007/s005990050081","DOIUrl":"https://doi.org/10.1007/s005990050081","url":null,"abstract":"Percutaneous transluminal coronary angioplasty is an established therapy for coronary artery disease, but restenosis still occurs at a rate of 25%-40%. The aim of this study was to investigate the acute effect of percutaneous transluminal coronary angioplasty on platelet function and the relationship between platelet function and clinical recurrence. Spontaneous platelet aggregation was assessed before and after successful coronary angioplasty in 155 patients (120 men, 35 women). Patients were followed for a mean time of 20 months; follow-up angiography was performed only in patients with clinical recurrence. In 122 of 155 patients (79%) a significant increase in spontaneous platelet aggregation was observed immediately after coronary angioplasty. Baseline spontaneous platelet aggregation in platelet-rich plasma was significantly lower in patients with clinical recurrence than in those without (P<0.05). Kaplan-Meier event-free survival estimate showed a significant difference in clinical recurrence between patients with spontaneous platelet aggregation in platelet-rich plasma below and above the first quintile (P<0.05) with a relative risk of 2.5. In conclusion. these results indicate that percutaneous transluminal coronary angioplasty enhances spontaneous platelet aggregation and that the platelet state before coronary angioplasty affects the risk of clinical recurrence after the procedure.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 4","pages":"145-9"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Correlation of low-density lipoprotein modification by myeloperoxidase with hypochlorous acid formation. 髓过氧化物酶修饰低密度脂蛋白与次氯酸形成的相关性。

International journal of clinical & laboratory research Pub Date : 1999-01-01 DOI: 10.1007/s005990050083

A Jerlich, L Horakova, J S Fabjan, A Giessauf, G Jürgens, R J Schaur

{"title":"Correlation of low-density lipoprotein modification by myeloperoxidase with hypochlorous acid formation.","authors":"A Jerlich, L Horakova, J S Fabjan, A Giessauf, G Jürgens, R J Schaur","doi":"10.1007/s005990050083","DOIUrl":"https://doi.org/10.1007/s005990050083","url":null,"abstract":"Myeloperoxidase is an enzyme in phagocytes which catalyzes several redox reactions. A major product is hypochlorous acid which appears to be important in inflammatory processes such as atherosclerosis. The aim of this study was to investigate whether the kinetics of low-density lipoprotein modification by the myeloperoxidase/hydrogen peroxide/chloride system in vitro conform to the established kinetics of hypochlorous acid formation and to compare the results with known in vivo data. The absorbance at 234 nm was applied to study the kinetics of the modification of low-density lipoprotein. Variation of the concentration of low-density lipoprotein, hydrogen peroxide, and chloride, respectively, had a biphasic effect on the maximal rate of low-density lipoprotein modification. Increasing the substrates up to certain threshold levels resulted in increased modification, however, further increases caused inhibition of low-density lipoprotein modification. The inhibitory effect of higher low-density lipoprotein concentrations might be relevant, since these concentrations occur in the human aortic intima. Furthermore, a positive correlation was found between the maximal rate of low-density lipoprotein modification and the acidity of the medium. In summary, low-density lipoprotein modification is affected by the myeloperoxidase/hydrogen peroxide/chloride system in a similar manner to hypochlorous acid production. We conclude that myeloperoxidase, which has been detected in atherosclerotic lesions, is able to modify low-density lipoprotein into the form which is taken up by macrophages in an uncontrolled manner.","PeriodicalId":77180,"journal":{"name":"International journal of clinical & laboratory research","volume":"29 4","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005990050083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21