International journal of cancer. Supplement = Journal international du cancer. Supplement最新文献_第5页

Adoptive immunotherapy of ovarian carcinoma with bs-MAb-targeted lymphocytes: a multicenter study. 卵巢癌的过继免疫治疗bs- mab靶向淋巴细胞:一项多中心研究

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

R L Bolhuis, C H Lamers, S H Goey, A M Eggermont, J B Trimbos, G Stoter, A Lanzavecchia, E di Re, S Miotti, F Raspagliesi

引用次数: 0

Janusin: new molecular design for bispecific reagents. Janusin:双特异性试剂的新分子设计。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

A Traunecker, A Lanzavecchia, K Karjalainen

{"title":"Janusin: new molecular design for bispecific reagents.","authors":"A Traunecker, A Lanzavecchia, K Karjalainen","doi":"","DOIUrl":"","url":null,"abstract":"It is well established that soluble CD4 (sCD4) inhibits HIV infection in vitro, regardless of the virus strain or genetic variant. Most effective molecules, thus far, based on sCD4 are those in which CD4 is combined with immunoglobulin constant regions (CD4-IgG or CD4-IgM). Such molecules maintained HIV-gp120 specificity mediated by CD4 and also antibody effector functions such as complement activation, Fc receptor binding, long serum half-life or transport across the placental barrier. We have now developed sCD4 molecules which are even more potent anti-HIV reagents. These molecules are based on the principle of bispecific antibodies and they have properties capable of retargeting cytotoxic T lymphocytes onto HIV-infected cells and inducing efficient killing. CD4 combined with anti-human CD3 (FvCD3) single-chain combining site has been produced (CD4-FvCD3-JANUSIN). This molecule shows the expected biological activities, namely, binding to the 2 ligands, human CD3 and gp120, also efficiently retargeting CTLs of any specificity onto HIV-infected cells. In addition, several advantages over classical bispecific antibodies can be achieved: only one polypeptide, not a mixture containing the desired product, is produced, thus simplifying the purification process. In addition, Janusin designs do not contain the Ig Fc portion, which could mediate illegitimate retargeting of T-cells. In addition to CD4-FvCD3-JANUSIN, receptor-Fv, Fv-Fv or ligand-Fv Janusins can be produced.","PeriodicalId":77178,"journal":{"name":"International journal of cancer. Supplement = Journal international du cancer. Supplement","volume":"7 ","pages":"51-2"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12598987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bispecific antibody therapy of two murine B-cell lymphomas. 两种小鼠b细胞淋巴瘤的双特异性抗体治疗。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

C Demanet, J Brissinck, M Moser, O Leo, K Thielemans

引用次数: 0

Activation of T cells in vivo using anti-CD3 and staphylococcal enterotoxins. 使用抗cd3和葡萄球菌肠毒素在体内激活T细胞。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

J A Bluestone, D S Bruce, K A Newell

引用次数: 0

CTLA-4 and CD28: similar proteins, neighbouring genes. CTLA-4和CD28:相似的蛋白质，邻近的基因。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

C Balzano, N Buonavista, E Rouvier, P Golstein

{"title":"CTLA-4 and CD28: similar proteins, neighbouring genes.","authors":"C Balzano, N Buonavista, E Rouvier, P Golstein","doi":"","DOIUrl":"","url":null,"abstract":"Subtractive cloning and screening yielded a cDNA clone corresponding to a molecule expressed in activated T cells, called CTLA-4. At the protein level, CTLA-4, a single-V-domain member of the immunoglobulin superfamily, was found very homologous to the lymphocyte activation molecule CD28. In particular, the hinge region included the hexamer MYPPPY, completely conserved for both molecules and in mice and humans. By immunizing mice with a human CTLA-4 peptide, an anti-CTLA-4 monoclonal antibody (MAb) was obtained, which enabled to establish the MW of the protein (26 and 40 kDa under reduced and non-reduced conditions respectively) and its preliminary tissue distribution. Also, CTLA-4 and CD28 were very similar at the message and at the gene structure level. The corresponding genes had previously been found to co-map on mouse chromosome IC and on human chromosome 2q33. We show that they can be found on the same yeast artificial chromosomes bearing human genomic DNA, and that they are 25 to 150 kb apart. These marked homologies and gene proximity strongly suggest that CTLA-4 and CD28 are the direct products of a duplication event, and raise the question of the function of CTLA-4.","PeriodicalId":77178,"journal":{"name":"International journal of cancer. Supplement = Journal international du cancer. Supplement","volume":"7 ","pages":"28-32"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12504551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bispecific-antibody-mediated targeting of radiolabeled bivalent haptens: theoretical, experimental and clinical results. 双特异性抗体介导的放射性标记二价半抗原靶向:理论、实验和临床结果。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

J M Le Doussal, J Barbet, M Delaage

{"title":"Bispecific-antibody-mediated targeting of radiolabeled bivalent haptens: theoretical, experimental and clinical results.","authors":"J M Le Doussal, J Barbet, M Delaage","doi":"","DOIUrl":"","url":null,"abstract":"Chemically conjugated bispecific (anti-cell surface antigen, anti-hapten) Fab'-Fab antibodies (Bs-MAbs) have been used to target 125I-, 111In- and 99mTc-labeled haptens to cell sub-sets. In vitro, bivalent haptens were found to bind more strongly than their monovalent analogs to the Bs-MAbs bound to (\"ordered\" on) the cell surface, or than to free (\"disordered\") Bs-MAbs: they are selective for cell-bound Bs-MAbs. In tumor-grafted nude mice models, the sequential injections of microgram amounts of Bs-MAb, and 1 day later, of microC amounts of bivalent haptens permits to sharply delineate small tumors (using a gamma camera), hours after injection. Further, the isotope biodistribution was found to be at least 3 times more selective for the tumor than that obtained with directly labeled anti-CEA F(ab)'2 or with monovalent haptens. This better in vivo selectivity of the 2-step targeting of bivalent haptens was also demonstrated in a pharmacokinetic study using therapeutic amounts of reagents. In primary-colon-carcinoma patients, a similar comparative immunoscintigraphy study confirmed the better selectivity of bivalent hapten targeting over direct targeting, on the basis of image quality and ex vivo tissue counting. In patients with medullary carcinoma of the thyroid, bivalent hapten targeting allowed us to confirm tumor extension and to find occult lesions. Interestingly, radio-immunoguided surgery was necessary to resect these small lesions. These experimental results, together with technological and theoretical considerations, suggest that Bs-MAb-mediated targeting of isotopes (or other agents) is one of the major ways to increase the clinical performance of MAb-based targeting diagnostic and therapeutic tools.","PeriodicalId":77178,"journal":{"name":"International journal of cancer. Supplement = Journal international du cancer. Supplement","volume":"7 ","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12598989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted cytokine production. 靶向细胞因子的产生。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

D M Segal, J H Qian, J A Titus, M B Moreno, A J George, C R Jost, I Kurucz, M el-Gamil, J R Wunderlich

引用次数: 0

Functional assembly of chimeric T-cell receptor chains. 嵌合t细胞受体链的功能组装。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

G Gorochov, J Lustgarten, T Waks, G Gross, Z Eshhar

{"title":"Functional assembly of chimeric T-cell receptor chains.","authors":"G Gorochov, J Lustgarten, T Waks, G Gross, Z Eshhar","doi":"","DOIUrl":"","url":null,"abstract":"We have generated cytotoxic T-cell hybridomas expressing chimeric T-cell receptors (cTCR) with an antibody-type specificity for the TNP hapten. Transfectants expressing the cTCR genes could mediate specific lysis of haptenated tumor cell lines of various types and secrete IL-2 upon stimulation with TNP modified cells. In a previous report, we showed that double-gene transfectants expressing either VHC alpha and VLC beta or VHC beta and VLC alpha could be activated by TNP-modified stimulator cells or TNP proteins immobilized on plastic. Single-chain transfectants (expressing VHC alpha or VHC beta alone) could be mainly activated by TNP-cells. We now report that transfection of chimeric VHC alpha gene into an alpha-chain-defective mutant restores the surface expression of the TCR/CD3 complex. In parallel, such transfectants regained the ability to respond to mitogen and anti-CD3 antibodies and responded weakly to TNP cells. Double gene transfectants, bearing 2 complementary chimeric chains, expressed high amounts of cTCR on their surface, sufficient to acquire sound anti-TNP reactivity. Cells expressing the VHC beta gene only were not functional and had no detectable surface TCR chains. Taken together, our results suggest that chimeric VHC alpha chains can pair with endogenous V beta C beta chains, but that there is preferential association between complementary chimeric chains, resulting in higher functional expression of the chimeric TCR.","PeriodicalId":77178,"journal":{"name":"International journal of cancer. Supplement = Journal international du cancer. Supplement","volume":"7 ","pages":"53-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12598988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and function of the CD16:zeta:gamma complex expressed on human natural-killer cells. CD16:zeta: γ复合物在人自然杀伤细胞上表达的结构和功能。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1992-01-01

E Vivier, M Ackerly, N Rochet, P Anderson

{"title":"Structure and function of the CD16:zeta:gamma complex expressed on human natural-killer cells.","authors":"E Vivier, M Ackerly, N Rochet, P Anderson","doi":"","DOIUrl":"","url":null,"abstract":"Natural-killer (NK) cells are large granular lymphocytes involved in host defense against tumor cells and virally-infected cells. In addition to natural cytotoxicity, NK cells can effect antibody-dependent cytotoxicity mediated by CD16 (Fc gamma RIIIA alpha). It has recently been shown that CD16 is associated with disulfide-linked dimers composed of 2 homologous sub-units, zeta and gamma. These transducing molecules are also associated with other multimeric cell-surface receptors such as the T-cell antigen receptor (CD3:TCR) complex (zeta and gamma) and the high-affinity Fc receptor for IgE (Fc epsilon RI) expressed on basophils and mast cells (gamma). These results show that distinct cell-surface receptors utilize common transducing sub-units, and emphasize the homology between the CD16, Fc epsilon RI and CD3:TCR complexes. However within the lymphoid cells, the gamma-gamma homodimer is preferentially expressed in NK cells and cytotoxic T cells, suggesting that specific combinations of these transducing dimers might sub-serve distinct signal-transducing functions, and contribute to lymphocyte heterogeneity.","PeriodicalId":77178,"journal":{"name":"International journal of cancer. Supplement = Journal international du cancer. Supplement","volume":"7 ","pages":"11-4"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12599082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breast-cancer etiology. Report of a working party for the Nordic Cancer Union. 乳腺癌的病因。北欧癌症联盟工作组的报告。

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1990-01-01

H O Adami, G Adams, P Boyle, M Ewertz, N C Lee, E Lund, A B Miller, H Olsson, M Steel, D Trichopoulos

引用次数: 0