Experimental and clinical immunogenetics最新文献_第3页

Genotyping Interleukin-10 High and Low Producers with Single-Tube Bidirectional Allele-Specific Amplification 用单管双向等位基因特异性扩增法分型白细胞介素-10高、低生产者

Experimental and clinical immunogenetics Pub Date : 2001-04-01 DOI: 10.1159/000049184

J. Karhukorpi, R. Karttunen

引用次数: 18

Interferon-Alpha Induces Transient Suppressors of Cytokine Signalling Expression in Human T Cells 干扰素- α诱导人T细胞细胞因子信号表达的瞬时抑制

Experimental and clinical immunogenetics Pub Date : 2001-04-01 DOI: 10.1159/000049186

C. Brender, M. Nielsen, C. Röpke, M. Nissen, A. Svejgaard, N. Billestrup, C. Geisler, N. Ødum

引用次数: 46

Nomenclature of the Human Immunoglobulin Heavy (IGH) Genes 人重免疫球蛋白(IGH)基因命名法

Experimental and clinical immunogenetics Pub Date : 2001-04-01 DOI: 10.1159/000049189

M. Lefranc

引用次数: 91

Lack of FasL Expression in Cultured Human Retinal Pigment Epithelial Cells FasL在培养的人视网膜色素上皮细胞中缺乏表达

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049085

C. G. Kæstel, H. Madsen, J. Prause, A. Jørgensen, Y. Liang, M. la Cour, G. Lui, N. Ødum, M. Nissen, C. Röpke

引用次数: 10

Can HLA Typing Predict the Outcome of Grass Pollen Immunotherapy? HLA分型能否预测草花粉免疫治疗的效果?

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049083

A. Fleva, M. Daniilidis, J. Sidiropoulos, K. Adam, A. Tourkantonis, J. Daniilidis, L. Hadzipetrou

{"title":"Can HLA Typing Predict the Outcome of Grass Pollen Immunotherapy?","authors":"A. Fleva, M. Daniilidis, J. Sidiropoulos, K. Adam, A. Tourkantonis, J. Daniilidis, L. Hadzipetrou","doi":"10.1159/000049083","DOIUrl":"https://doi.org/10.1159/000049083","url":null,"abstract":"Objective: The aim of this study was to investigate the relationship between HLA molecules and the positive or negative response of atopic patients to specific immunotherapy (SIT). Methods: We studied 42 atopic multisensitive patients undergoing grass pollen immunotherapy, 42 parents of patients (30 mothers and 12 fathers) and 173 control individuals. HLA class I and class II antigens were typed by a microlymphocytotoxicity test. The typing of DRB1* alleles for atopic patients and their parents was based on the reverse hybridization principle, while for the control group, DNA-RFLP and PCR-SSP methods were used. Results: The frequency of B14 and DRB1*1101-4 antigens/alleles, as well as the A2B5DR11 haplotype, showed a statistically significant difference in those patients who responded to immunotherapy. On the other hand, HLA-A28, B8 and DRB1*0301 antigens/alleles, as well as the frequency of the A1B8 and A1B8DR3 haplotypes, were found to be significantly higher in patients who responded poorly to SIT. Discussion: Our findings support the hypothesis that treatment responsiveness may show an association to HLA molecules, which could thus play a role in the immunological selection and monitoring of atopic patient candidacy for SIT.","PeriodicalId":77124,"journal":{"name":"Experimental and clinical immunogenetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000049083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64616953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Protein Phosphatase 2A Isotypes Regulate Cell Surface Expression of the T Cell Receptor 蛋白磷酸酶2A同型调节T细胞受体的细胞表面表达

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049084

J. P. Lauritsen, C. Menné, J. Kastrup, J. Dietrich, C. Geisler

引用次数: 6

Genomic Analysis of Idiopathic Infertile Patients with Sperm-Specific Depletion of CD46 精子特异性CD46缺失的特发性不孕症患者的基因组分析

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049086

M. Nomura, M. Kitamura, K. Matsumiya, A. Tsujimura, A. Okuyama, M. Matsumoto, K. Toyoshima, T. Seya

{"title":"Genomic Analysis of Idiopathic Infertile Patients with Sperm-Specific Depletion of CD46","authors":"M. Nomura, M. Kitamura, K. Matsumiya, A. Tsujimura, A. Okuyama, M. Matsumoto, K. Toyoshima, T. Seya","doi":"10.1159/000049086","DOIUrl":"https://doi.org/10.1159/000049086","url":null,"abstract":"Three infertile subjects with no expression of CD46 (membrane cofactor protein of complement) on their spermatozoa were found when screening 542 idiopathic male infertile patients. The sperm CD46 isoform was reported to be associated with the sperm-egg interaction, yet a ubiquitous expression of CD46 confers resistance to complement-mediated injury on host cells. All three patients expressed normal CD46 isoforms on their lymphocytes and granulocytes. Thus, the loss of CD46 is sperm-specific, probably due to testicular germ cell-specific regulation of CD46 production. Recently, a mechanism of the gene regulation of human CD46 was elucidated in which the silencer element of the 3′ UT and the promoter region of human CD46 gene partly participate. Here, we analyzed these regions of the CD46 gene in our 3 patients. We found no abnormality in 3′ and 5′ regions of the CD46 genome in the 3 patients. Thus, in these infertile patients sperm-specific depletion of CD46 is not governed by the so for identified regulators in the CD46 gene. Other unknown factors outside the known regulatory regions would play a role in the regulation of sperm-specific CD46 expression.","PeriodicalId":77124,"journal":{"name":"Experimental and clinical immunogenetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000049086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64617594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Immunoglobulin GM and KM allotypes in systemic lupus erythematosus. 系统性红斑狼疮的免疫球蛋白 GM 和 KM 异型。

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049190

J P Pandey, G S Cooper, E L Treadwell, G S Gilkeson, E W St Clair, M A Dooley

引用次数: 0

Anti-fibrillin-1 autoantibodies in systemic sclerosis are GM and KM allotype-restricted. 系统性硬化症中的抗纤连蛋白-1自身抗体受GM和KM异型限制。

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049191

J P Pandey, G P Page, R M Silver, E C LeRoy, C A Bona

{"title":"Anti-fibrillin-1 autoantibodies in systemic sclerosis are GM and KM allotype-restricted.","authors":"J P Pandey, G P Page, R M Silver, E C LeRoy, C A Bona","doi":"10.1159/000049191","DOIUrl":"10.1159/000049191","url":null,"abstract":"<p><p>GM and KM allotypes--genetic markers of immunoglobulin (Ig) gamma and kappa chains, respectively--have been shown to play an important role in genetic predisposition to some autoimmune diseases. To determine their role in susceptibility to systemic sclerosis (SSc; scleroderma) and in the generation of anti-fibrillin-1 antibodies, 148 SSc patients and 191 controls were typed for several GM and KM allotypes by a standard hemagglutination inhibition method. IgG and IgM antibodies to fibrillin-1 were measured by radioimmunoassay. GM and KM phenotypes were not significantly associated with SSc. However, these determinants significantly influenced the production of anti-fibrillin-1 antibodies in SSc patients. In Caucasians, GM1,3,17 23 5,13,21 and GM3 23 5,13 phenotypes were associated with the presence and absence of IgG autoantibodies, respectively. The production of these autoantibodies was also associated with KM allotypes, KM1,3 heterozygosity being associated with response and homozygosity for the KM3 allele with nonresponse to fibrillin-1. In African-Americans, the KM1 homozygotes were associated with the absence of anti-fibrillin-1 antibodies and the KM3 homozygotes with the presence of autoantibodies. In this ethnic group, the GM1,17 5,13 phenotype was associated with the absence of IgM autoantibodies. This represents the first description of genetic control of autoimmunity to fibrillin-1 in scleroderma.</p>","PeriodicalId":77124,"journal":{"name":"Experimental and clinical immunogenetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64621415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Analysis of Plasma α1,3-Fucosyltransferase Deficiency and Development of the Methods for Its Genotyping 血浆α1,3-聚焦转移酶缺乏的分子分析及其基因分型方法的发展

Experimental and clinical immunogenetics Pub Date : 2001-01-01 DOI: 10.1159/000049082

Susumu Tanaka, S. Yazawa, Kasumi Noguchi, T. Nishimura, K. Miyanaga, N. Kochibe, D. Poland, W. van Dijk, K. Matta

{"title":"Molecular Analysis of Plasma α1,3-Fucosyltransferase Deficiency and Development of the Methods for Its Genotyping","authors":"Susumu Tanaka, S. Yazawa, Kasumi Noguchi, T. Nishimura, K. Miyanaga, N. Kochibe, D. Poland, W. van Dijk, K. Matta","doi":"10.1159/000049082","DOIUrl":"https://doi.org/10.1159/000049082","url":null,"abstract":"Four patients with mental illness were found to be deficient in plasma α1,3-fucosyltransferase for the first time in Japan [Exp Clin Immunogenet 1999;16:125–130]. Complete sequencing of FUT6 genes in these individuals revealed the presence of two point mutations, i.e., G739 to A (Glu→247 to Lys) and C945 to A (Tyr→315 to stop). In addition to two reported alleles having G739 to A (pf1) and G739 to A and C945 to A (pf3), a new mutated allele having C945 to A (pf2) was found to be present and all the individuals who lack α1,3-fucosyltransferase activity in plasma were found to possess pf genes homozygously (pf/pf). In order to detect such lethal mutations in FUT6 genes easily, PCR-RFLP methods have also been developed and applied for the screening of FUT6 deficiency in a large number of samples which resulted in the demonstration of three additional FUT6-deficient individuals. The absence of α1,3-fucosylated molecules on α1-acid glycoprotein in plasma from all the 7 individuals was confirmed to result from the plasma α1,3-fucosyltransferase deficiency.","PeriodicalId":77124,"journal":{"name":"Experimental and clinical immunogenetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000049082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64616891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12