European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies最新文献_第2页

The optimized use of gas chromatography-mass spectrometry and high performance liquid chromatography to analyse the serum bile acids of patients with metabolic cholestasis and peroxisomal disorders. 气相色谱-质谱联用和高效液相色谱分析代谢性胆汁淤积和过氧化物酶体疾病患者血清胆汁酸的优化方法。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01 DOI: 10.1515/cclm.1997.35.12.919

F Courillon, M F Gerhardt, A Myara, F Rocchiccioli, F Trivin

{"title":"The optimized use of gas chromatography-mass spectrometry and high performance liquid chromatography to analyse the serum bile acids of patients with metabolic cholestasis and peroxisomal disorders.","authors":"F Courillon, M F Gerhardt, A Myara, F Rocchiccioli, F Trivin","doi":"10.1515/cclm.1997.35.12.919","DOIUrl":"https://doi.org/10.1515/cclm.1997.35.12.919","url":null,"abstract":"We have measured the bile acids in human serum as methyl ester-trimethylsilyl ethers by gas chromatography-mass spectrometry (GC-MS) using an electron ionization procedure. The overall method was validated and the detection limit (0.4 mumol/l), linearity (2-30 mumol/l), intra-day and inter-day precision, accuracy and recovery (96.2% for nor-23-deoxycholic acid as internal standard) were measured. Serum C24-bile acids profiles from 43 cholestatic patients were measured by GC-MS and by HPLC. The results obtained with the two methods were well correlated and the criteria for selecting either HPLC or GC-MS identified. The serum C24- and C27-bile acids and C29 dicarboxylic bile acid profiles for patients with generalized peroxisomal deficiencies, like Zellweger syndrome (n = 5), neonatal adrenoleukodystrophy (n = 1), infantile Refsum disease (n = 2) and from a single peroxisomal deficiency (n = 1) were also measured by GC-MS.","PeriodicalId":77119,"journal":{"name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","volume":"35 12","pages":"919-22"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1997.35.12.919","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20402522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Determination of HbA1c in the presence of haemoglobin variants: comparison of three HPLC techniques. 血红蛋白变异时HbA1c的测定:三种HPLC技术的比较。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01

M Carta, G Dall'Olio, G Soffiati

引用次数: 0

Determination of plasma lipid hydroperoxides by an NADPH/NADP+ coupled enzyme reaction system. Evaluation of a method. NADPH/NADP+偶联酶反应体系测定血浆脂质氢过氧化物。对一种方法的评估

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01 DOI: 10.1515/cclm.1997.35.12.893

C Ruiz, A Alegría, R Barberá, R Farré, M J Lagarda

引用次数: 7

Concentration of the cross-linked carboxyterminal telopeptide of type I collagen in serum of young growing rats fed a low calcium and vitamin D-deficient diet. 低钙缺乏维生素d的幼龄大鼠血清中I型胶原交联羧基末端末端肽的浓度。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01 DOI: 10.1515/cclm.1997.35.12.915

A R Bielaczyc, M Gołebiewska, A Citko, F Rogowski

{"title":"Concentration of the cross-linked carboxyterminal telopeptide of type I collagen in serum of young growing rats fed a low calcium and vitamin D-deficient diet.","authors":"A R Bielaczyc, M Gołebiewska, A Citko, F Rogowski","doi":"10.1515/cclm.1997.35.12.915","DOIUrl":"https://doi.org/10.1515/cclm.1997.35.12.915","url":null,"abstract":"Type I collagen is the main type found in mineralized bone. Specific radioimmunoassay for the cross-linked carboxyterminal telopeptide of type I collagen allows assessing the degradation of type I collagen in serum samples. The aim of the present investigation was to determine the concentration of cross-linked carboxyterminal telopeptide of type I collagen in serum of dietary calcium and vitamin D-deficient rats, a good model disease of decreased formation and mineralization of bone matrix and excessive bone resorption. The studies were carried out on 20 young growing Wistar rats. Serum concentration of the cross-linked carboxyterminal telopeptide of type I collagen was analyzed by the Rat Telopeptide [125I]ICTP Radioimmunoassay Kit obtained from Orion Diagnostica (Finland). The data obtained from biochemical analysis showed increased concentration of the cross-linked carboxyterminal telopeptide of type I collagen in the serum of rats fed a low calcium and vitamin D-deficient diet after 14 days of the experiment. At the end of the experiment (day 21), the concentration of carboxyterminal telopeptide of type I collagen in serum was still elevated in these animals. In conclusion, dietary calcium and vitamin D-deficiency in rats produces hypocalcaemia together with the increased concentration of the cross-linked carboxyterminal telopeptide of type I collagen in serum.","PeriodicalId":77119,"journal":{"name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","volume":"35 12","pages":"915-8"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1997.35.12.915","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20402521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of storage on venous and capillary blood samples: the influence of deferoxamine and butylated hydroxytoluene on the fatty acid alterations in red blood cell phospholipids. 静脉和毛细血管血样储存的影响:去铁胺和丁基羟基甲苯对红细胞磷脂脂肪酸改变的影响。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01 DOI: 10.1515/cclm.1997.35.12.907

S J Otto, M M Foreman-von Drongelen, A C von Houwelingen, G Hornstra

{"title":"Effects of storage on venous and capillary blood samples: the influence of deferoxamine and butylated hydroxytoluene on the fatty acid alterations in red blood cell phospholipids.","authors":"S J Otto, M M Foreman-von Drongelen, A C von Houwelingen, G Hornstra","doi":"10.1515/cclm.1997.35.12.907","DOIUrl":"https://doi.org/10.1515/cclm.1997.35.12.907","url":null,"abstract":"Fatty acid concentrations in plasma and red blood cell phospholipids isolated from paired venous and capillary blood samples were compared and the effect of storage at -20 degrees C was evaluated as well. Plasma fatty acid profiles from venous and capillary blood were found to be comparable and not affected by up to four weeks of storage, while fatty acid profiles of venous and capillary red blood cells were no longer comparable after four weeks. Substantial losses of long-chain polyunsaturated fatty acids were observed in capillary red blood cells. To investigate whether the observed long-chain polyunsaturated fatty acids loss could be prevented, capillary red blood samples were stored for up to one year at -50 degrees C in the presence of the iron-binding agent deferoxamine or the free radical scavenger butylated hydroxytoluene. Both compounds protected the long-chain polyunsaturated fatty acids. Similarly, storage of red blood cell lipid extracts at -50 degrees C for up to one year was not associated with reduced levels of long-chain polyunsaturated fatty acids. In conclusion, the lipid loss from capillary red blood cells can be reduced for at least one year during storage at -50 degrees C with prior addition of either a metal chelating compound or a free radical scavenger, or by preparing lipid extracts of the samples within one week of blood collection.","PeriodicalId":77119,"journal":{"name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","volume":"35 12","pages":"907-13"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1997.35.12.907","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20402520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

"Ocean-to-Ocean Project": a transcontinental external quality assessment trial in clinical chemistry realized throughout Eurasia. “洋对洋项目”:横跨欧亚大陆的临床化学外部质量评估试验。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01

J Kratochvíla, M Budina, H Baadenhuijsen, A Moshkin, E Legenstein, E Kaiser, B Friedecký, P Schneiderka, A Lapin

{"title":"\"Ocean-to-Ocean Project\": a transcontinental external quality assessment trial in clinical chemistry realized throughout Eurasia.","authors":"J Kratochvíla, M Budina, H Baadenhuijsen, A Moshkin, E Legenstein, E Kaiser, B Friedecký, P Schneiderka, A Lapin","doi":"","DOIUrl":"","url":null,"abstract":"More than 800 diagnostic laboratories situated throughout the Eur-Asian continent--from the Pacific Coast up to the North Sea littoral--were involved in a common survey of External Quality Assessment (EQA). It consisted of the simultaneous measurement of up to 30 analytes of 'general' clinical chemistry using the same batch of control material. The laboratories were associated in four EQA institutions: SKZL (The Netherlands), OQUASTA (Austria), SEKK (Czech Republic) and BKKSystem (Community of Independent States). The results demonstrated the feasibility of such a large-scale survey and provided a realistic idea about the state-of-the-art of laboratory diagnosis in these countries: Besides some local specific problems, such as poor quality of water or the forced use of reagents and calibrators from different sources, there are general problems hindering an efficient process of 'harmonization' in laboratory medicine, namely, the high methodological dispersion especially in the case of enzymes and of some organic analytes. At the same time there is a potential necessity for more concentrated implementation of internal quality assessment into the routine work of laboratories.","PeriodicalId":77119,"journal":{"name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","volume":"35 12","pages":"927-35"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20402524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical laboratory sciences versus laboratory medicine. 临床检验科学与检验医学。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01

X Fuentes-Arderiu

引用次数: 0

Award of the Sarstedt Research Prize 1997. 1997年萨斯泰特研究奖。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01

C Wagener, F Körber

引用次数: 0

Hepatitis C virus (HCV) specific sequences are demonstrable in the DNA fraction of peripheral blood mononuclear cells from healthy, anti-HCV antibody-negative individuals and cell lines of human origin. 丙型肝炎病毒(HCV)特异性序列在健康、抗HCV抗体阴性个体和人源细胞系的外周血单个核细胞的DNA片段中得到证实。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-12-01 DOI: 10.1515/cclm.1997.35.12.899

R H Dennin, Z Chen

{"title":"Hepatitis C virus (HCV) specific sequences are demonstrable in the DNA fraction of peripheral blood mononuclear cells from healthy, anti-HCV antibody-negative individuals and cell lines of human origin.","authors":"R H Dennin, Z Chen","doi":"10.1515/cclm.1997.35.12.899","DOIUrl":"https://doi.org/10.1515/cclm.1997.35.12.899","url":null,"abstract":"No convincing support has been provided so far for the existence of extrahepatic hepatitis C virus particles that should correspond to the sometimes extremely high concentration of 'HCV-RNA' in serum or plasma. If a naturally occurring HCV-specific DNA were to be found, a concept for at least some phenomena in terms of the pathophysiology of HCV should become conceivable. DNA was extracted from peripheral blood mononuclear cells of eleven healthy, anti-HCV-negative individuals, including five long term blood donors, and cells from different cell lines. DNA was subjected to nested polymerase chain reaction omitting a reverse transcriptase step with primers of the 5'NC as well as part of the core region of HCV. Direct polymerase chain reaction, i.e. without a reverse transcriptase step, revealed HCV-specific sequences in the DNA fraction of peripheral blood mononuclear cells of different origin: healthy anti-HCV negative individuals, furthermore in HeLa and MT2 cells. The fragments found were of expected length as well as of shorter and of longer than expected length with respect to the sequence of the HCV genome framed by the primers applied. The results derived from additional hybridization, restriction endonuclase analysis, and sequencing demonstrated HCV-specific sequences in the expected fragments with both a high degree of homology and deletions, respectively, substitutions, as compared to a prototype strain. However, the longer than expected fragments also contained sequences not specific for HCV.","PeriodicalId":77119,"journal":{"name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","volume":"35 12","pages":"899-905"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1997.35.12.899","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20402519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The first fully automated allergy analyser UniCAP: comparison with IMMULITE for allergy panel testing. 第一个全自动过敏分析仪UniCAP:与IMMULITE过敏面板测试的比较。

European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Pub Date : 1997-11-01

G M Costongs, B M Bas

{"title":"The first fully automated allergy analyser UniCAP: comparison with IMMULITE for allergy panel testing.","authors":"G M Costongs, B M Bas","doi":"","DOIUrl":"","url":null,"abstract":"Automated immunoassay systems should be convenient to handle, flexible and give reliable results. To investigate the extent to which the UniCAP System met the above requirements, compared with the IMMULITE System, we compared the Phadiatop (UniCAP) and AlaTOP (IMMULITE) results of 110 patients with positive clinical diagnoses for inhalant allergy. In addition, we compared food screening test results of 103 patients with a clinical positive diagnosis for food, and 110 test results of controls with negative diagnosis for allergy. Phadiatop had a sensitivity of 96% and a specificity of 92%. AlaTOP had a sensitivity of 86% and a specificity of 94%. For food screening the results were: 75% sensitivity and 82% specificity for fx5 (UniCAP) and 63% sensitivity and 71% specificity for fp5 (IMMULITE). Furthermore, those samples for which the test results which were not in concordance with the clinical diagnosis were tested with the follow-up panel of the different screening tests. For the AlaTOP follow-up we had to use the DPC microplate System (Milenia), because single allergen testing is not yet possible on the IMMULITE System. With regard to sensitivity, the UniCAP specific inhalant allergen tests and the original Phadiatop results showed closer agreement with each other than did the Milenia specific allergen results with the AlaTOP. The specificity of the single inhalant allergen tests was the same for both systems. For food allergy testing the UniCAP System shows closer agreement between the screening and the follow-up results than does the IMMULITE. The hands on time for loading 44 samples was practically the same for both systems, but for the follow-up tests the Milenia System is used next to the IMMULITE. Therefore from a logistical point of view the UniCAP System is more convenient. From these results we conclude that both logistically and clinically UniCAP seems to meet our requirements better than the IMMULITE.","PeriodicalId":77119,"journal":{"name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","volume":"35 11","pages":"885-8"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20354036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0