Epithelial cell biology最新文献_第3页

Crypt and villous epithelial cells in adult rat small intestine: numerical and volumetric variation along longitudinal and vertical axes. 成年大鼠小肠隐窝和绒毛上皮细胞:纵向和纵向的数量和体积变化。

Epithelial cell biology Pub Date : 1994-07-01

S A Zoubi, T M Mayhew, R A Sparrow

{"title":"Crypt and villous epithelial cells in adult rat small intestine: numerical and volumetric variation along longitudinal and vertical axes.","authors":"S A Zoubi, T M Mayhew, R A Sparrow","doi":"","DOIUrl":"","url":null,"abstract":"Previous studies on the small intestines of certain adult rodents have suggested that the total number of crypt epithelial cells is equal to the number of villous epithelial cells. This possibility is examined on adult male Sprague-Dawley rats using design-based stereological methods. Small intestines were removed and divided into four segments of equal length. For each segment, numbers and mean volumes of epithelial cells in crypts and villi were estimated and then combined to give values for the entire intestine. Variations along the vertical (crypt-villus) axis and the intestinal long (pyloro-ileocaecal) axis were examined. Cell numbers in crypts and on villi varied from segment to segment. Villus:crypt cell ratios proximally were roughly double those found distally. The ratio for the entire intestine was, on average, 1.6 villous cells per crypt cell. These results demonstrate that numbers of crypt and villous cells need not be equivalent. Previous findings may reflect the fact that ratios fluctuate according to circumstances (e.g., with species, age, nutritional status, disease, etc) or may be due to technical differences in tissue sampling and morphometry. Cell volumes varied along the crypt-villus axis as well as between segments, confirming known gradients of maturation and activity. Finally, the size of the intestinal epithelial proliferon was calculated in terms of the average numbers of epithelial cells per villus and of crypt epithelial cells feeding on to it.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"3 3","pages":"112-8"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18894971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative ultrastructural study of acrylamide induced cytoplasmic desmosome-like structures in cultured rat keratinocytes. 丙烯酰胺诱导大鼠角质形成细胞胞质桥粒样结构的定量超微结构研究。

Epithelial cell biology Pub Date : 1994-07-01

A H Shabana, M Oboeuf, N Forest

{"title":"Quantitative ultrastructural study of acrylamide induced cytoplasmic desmosome-like structures in cultured rat keratinocytes.","authors":"A H Shabana, M Oboeuf, N Forest","doi":"","DOIUrl":"","url":null,"abstract":"Although present in many tumours, the mechanism of formation and the significance of the so-called 'cytoplasmic desmosome' (CD) remain speculative. Recently, we reported an in vitro model for the induction of CD in rat keratinocytes following acrylamide treatment. In the present study and based on quantitative and qualitative evidence, CD could be divided into two categories. The first comprised individually scattered desmosomes always located in the cortical cytoplasm and associated with tonofilament bundles. Their sizes were comparable with those of intercellular desmosomes (ID) in control cells. The second category comprised clusters of homogeneously small-size desmosomes that may be located deep in the cytoplasm and were not always associated with tonofilament bundles. Whilst the latter group may be formed de novo in the cytoplasm as a result of the acrylamide-induced inhibition of protein kinases, the first group may be the result of internalization of surface desmosomes by certain tonofilaments under tension. In order to assess this possibility, we examined tonofilament organization following wounding of the epithelial sheet. Injured cells exhibited spiral-form tonofilaments extending close to the cell membrane. This retraction could be justified ultrastructurally by direct association between tonofilaments and microfilaments.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"3 3","pages":"102-11"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18894970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Derivation of conditionally immortalized cell lines containing the Min mutation from the normal colonic mucosa and other tissues of an "Immortomouse"/Min hybrid. 从“永生化小鼠”/Min杂交的正常结肠黏膜和其他组织中衍生出含有Min突变的条件永生化细胞系。

Epithelial cell biology Pub Date : 1994-07-01

R H Whitehead, J L Joseph

引用次数: 0

Highly polarized primary urothelial cells from human ureter grown as spheroid-like vesicles. 来自人输尿管的高度极化的初代尿路上皮细胞生长成球状囊。

Epithelial cell biology Pub Date : 1994-07-01

H J Boxberger, M J Sessler, B Maetzel, I M Mosleh, H D Becker, T F Meyer

引用次数: 0

Expression of keratins K6 and K16 in regenerating mouse epidermis is less restricted by cell replication than the expression of K1 and K10. 与K1和K10的表达相比，再生小鼠表皮中角蛋白K6和K16的表达受细胞复制的限制较小。

Epithelial cell biology Pub Date : 1994-07-01

A Heyden, C Lützow-Holm, O P Clausen, P Brandtzaeg, H S Huitfeldt

{"title":"Expression of keratins K6 and K16 in regenerating mouse epidermis is less restricted by cell replication than the expression of K1 and K10.","authors":"A Heyden, C Lützow-Holm, O P Clausen, P Brandtzaeg, H S Huitfeldt","doi":"","DOIUrl":"","url":null,"abstract":"Biosynthesis of the hyperproliferation-associated keratins K6 and K16 was studied in mouse epidermis following a single topical application of the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). An epidermal cell cohort was followed by pulse-labelling with the thymidine analogue 5-bromodeoxyuridine (BrdUrd). Mice were injected intraperitoneally with BrdUrd 1 h before a single topical application of TPA. TPA induced regenerative epidermal hyperplasia with hyperproliferation and shortened suprabasal transit time. The BrdUrd-labelled cell cohort was followed for 96 h after TPA-treatment by two-colour immunofluorescence staining with antibodies to BrdUrd, keratin K6 and K16. In control animals, K6 and K16 were found only in the hair follicles. K6 expression was immediately induced in all epidermal cell layers of TPA-treated epidermis, including actively replicating cells and it was expressed during the whole observation period. K16 was only present in post-mitotic cells and was transiently expressed 8-72 h after TPA treatment. Our results suggest that the expression of K6 and K16 is less restricted by cellular replication than the normally occurring K1 and K10 keratins.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"3 3","pages":"96-101"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18541096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epithelial monolayers from human epididymal and efferent duct tubules; testosterone metabolism and effects of culture conditions on cell height and confluence. 人附睾和传出管小管上皮单层;睾酮代谢及培养条件对细胞高度和融合的影响。

Epithelial cell biology Pub Date : 1994-07-01

S Raczek, C H Yeung, A Wagenfeld, L Hertle, H Schulze, T G Cooper

引用次数: 0

Histochemical study of human nasopharyngeal epithelium by horseradish peroxidase conjugated lectins. 辣根过氧化物酶结合凝集素对人鼻咽上皮组织化学的研究。

Epithelial cell biology Pub Date : 1994-01-01

M Gulisano, S Gheri Bryk, G Gheri, E Sgambati, A Curreli, W Masala, P Pacini

引用次数: 0

Cytokeratin expression in epithelial cells isolated from the crypt and villus regions of the rodent small intestine. 从啮齿动物小肠隐窝和绒毛区分离的上皮细胞中细胞角蛋白的表达。

Epithelial cell biology Pub Date : 1994-01-01

N Flint, P W Pemberton, R W Lobley, G S Evans

{"title":"Cytokeratin expression in epithelial cells isolated from the crypt and villus regions of the rodent small intestine.","authors":"N Flint, P W Pemberton, R W Lobley, G S Evans","doi":"","DOIUrl":"","url":null,"abstract":"Many physiological and structural features of epithelium in the small intestine are regulated during their transit from the crypt base to the villus tip. This crypt-villus axis is an important model for the study of the regulation of cell proliferation and differentiation. We have investigated the expression of cytokeratins in purified epithelial cells from the proliferative (crypt) and differentiated (villus) regions of this tissue. Three polypeptides were identified (cytokeratins 8, 18 and 19) as well as a fourth, 46 kDa polypeptide with similar electrophoretic characteristics to the recently identified cytokeratin 20. The distribution of these molecules was found to vary along the crypt-villus axis, with cytokeratin 18 being restricted to the proliferative crypt and cytokeratins 8 and 19 demonstrating more uniform distributions. The 46 kDa component was found to be expressed predominantly within the villus epithelium. Although there is no substantial evidence of a direct role for cytokeratins in the process of epithelial differentiation, these data suggest that differential expression of cytokeratins is associated with changes in intestinal epithelial differentiation.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"3 1","pages":"16-23"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18521897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of cytokeratin proteins during tumour progression in oral mucosa. 口腔黏膜肿瘤进展过程中细胞角蛋白的差异表达。

Epithelial cell biology Pub Date : 1994-01-01

S Kannan, P Balaram, G J Chandran, M R Pillai, B Mathew, K R Nalinakumari, M K Nair

{"title":"Differential expression of cytokeratin proteins during tumour progression in oral mucosa.","authors":"S Kannan, P Balaram, G J Chandran, M R Pillai, B Mathew, K R Nalinakumari, M K Nair","doi":"","DOIUrl":"","url":null,"abstract":"The expression pattern of cytokeratin filaments in epithelia has been shown to be dependent on their type and grade of differentiation. The type of expression of cytokeratin in a cell may also be altered during carcinogenesis or other pathological conditions. The present study examined the alterations in expression of various cytokeratin types during different stages of tumour progression in the oral mucosa. Six monoclonal anti-cytokeratin antibodies were used for the study. Conspicuous staining differences with these antibodies were evident between normal keratinizing and non-keratinizing mucosa. These differences can be correlated to the differentiation pattern of the normal mucosa types. Antibodies specific to cytokeratin types 10/11, 19 and 14 showed significant correlation with stage of tumour progression. In addition cytokeratin type 18 also showed prominent differences in expression between different stages of tumour progression. These alterations in cytokeratin expression suggest that the terminal differentiation pathway of keratinocytes is disturbed during oral carcinogenesis. The results of the present study also emphasize the potential of using cytokeratin filaments as markers in the biological staging of oral premalignant and malignant lesions.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"3 2","pages":"61-9"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18536249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional activity and ultrastructure of morphologically different types of nucleoli isolated from hepatocytes of normal and hepatectomized rats. 正常和去肝大鼠肝细胞不同形态核仁的转录活性和超微结构。

Epithelial cell biology Pub Date : 1994-01-01

D V Dzidziguri, P V Chelidze, M A Zarandia, E C Cherkezia, G D Tumanishvili

引用次数: 0