Epithelial cell biology最新文献_第9页

Identification of an 85-100 kDa glycoprotein as a cell surface marker for an advanced stage of urothelial differentiation: association with the inter-plaque ('hinge') area. 鉴定85-100 kDa糖蛋白作为尿路上皮分化晚期的细胞表面标志物:与斑块间(“铰链”)区域相关。

Epithelial cell biology Pub Date : 1992-01-01

J Yu, M Manabe, T T Sun

{"title":"Identification of an 85-100 kDa glycoprotein as a cell surface marker for an advanced stage of urothelial differentiation: association with the inter-plaque ('hinge') area.","authors":"J Yu, M Manabe, T T Sun","doi":"","DOIUrl":"","url":null,"abstract":"Although bladder cancers account for almost 5% of all human cancer deaths, little is known about the biochemistry of urothelial differentiation. We have recently identified three major protein subunits ('uroplakins') of asymmetric unit membranes (AUM), which form rigid-looking plaques covering up to 70% of the apical surface of urothelial superficial (umbrella) cells. The ordinary-looking plasma membranes that interconnect these plaques are believed to be functionally specialized, serving as flexible but durable 'hinges'. Whether these hinge membranes are biochemically unique is unknown. Using a new monoclonal antibody (AE32) we have identified an 85-100 kDa glycoprotein (UGP85) which appears to be urothelium-specific. In both normal urothelium and cultured urothelial colonies this cell surface protein is associated mainly with superficial cells, suggesting that its expression is differentiation dependent. Results from in vitro translation experiments indicated that this glycoprotein contains a core polypeptide of about 55 kDa. Using immunogold localization techniques, we showed that in cultured urothelial colonies--which are known to lack mature AUM plaques--UGP85 is distributed relatively uniformly on the apical surface of some differentiated cells. However, in superficial cells of normal urothelium UGP85 is mainly associated with the hinge areas. These results raise the possibility that UGP85 is a plasma membrane component which can be excluded, to varying extents, from the plaque region as 12 nm protein particles are assembled into a tightly packed paracrystalline AUM structure. The identification of UGP85 provides the first evidence that the hinge areas interconnecting the urothelial plaques are biochemically distinguishable from the plasma membranes of the relatively undifferentiated urothelial cells of the lower cell layers.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"1 1","pages":"4-12"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12481753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of ornithine decarboxylase messenger RNA in human hepatocellular carcinoma by in situ hybridization. 原位杂交法检测人肝癌中鸟氨酸脱羧酶信使RNA。

Epithelial cell biology Pub Date : 1992-01-01

F Y Gan, M S Gesell, J A Moshier, M Alousi, G D Luk

{"title":"Detection of ornithine decarboxylase messenger RNA in human hepatocellular carcinoma by in situ hybridization.","authors":"F Y Gan, M S Gesell, J A Moshier, M Alousi, G D Luk","doi":"","DOIUrl":"","url":null,"abstract":"Ornithine decarboxylase (ODC) has been shown by biochemical analysis, to be important for cell proliferation and carcinogenesis in a variety of tissues, including the liver. We detected messenger RNA (mRNA) specific for the enzyme ODC in 18 patients with hepatocellular carcinoma by an in situ hybridization technique using a radiolabelled ODC probe on formalin-fixed liver specimens. Adjacent uninvolved liver tissues were used as controls. Among the adjacent uninvolved liver tissues, five showed evidence of cirrhosis. Poorly differentiated hepatocellular carcinoma has significantly higher levels of ODC mRNA than does well-differentiated hepatocellular carcinoma, which in turn has a significantly higher ODC mRNA level than adjacent uninvolved liver tissues; tissues showing evidence of cirrhosis, on the other hand, had a significantly lower ODC mRNA level than adjacent uninvolved liver tissue. This pattern of ODC gene expression in hepatocellular carcinoma is similar to the pattern of expression of other oncogenes in liver tumours. The quantitative detection of ODC mRNA in hepatocellular carcinoma by in situ hybridization may help elucidate the potential role of ODC in hepatocarcinogenesis.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"1 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12512683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of stroma in oestrogen-induced epithelial proliferation. 基质在雌激素诱导的上皮细胞增殖中的作用。

Epithelial cell biology Pub Date : 1992-01-01

G R Cunha, P Young

{"title":"Role of stroma in oestrogen-induced epithelial proliferation.","authors":"G R Cunha, P Young","doi":"","DOIUrl":"","url":null,"abstract":"To examine the role of stromal-epithelial interactions in the response of epithelial cells to oestrogens, tissue recombinations were prepared with epithelium (E) and stroma (S) from the vagina (V) and urinary bladder (BL), that is between oestrogen target tissues (VS and VE) and non-target tissues (BLS and BLE). Following 3 weeks of growth in intact female hosts, ovariectomy was performed and 1 week later the hosts subjected to various hormonal treatments. Whereas homotypic vaginal tissue recombinations (VS+VE) exhibited epithelial cornification and mucification (cycling), this activity was not observed in homotypic bladder recombinants (BLS+BLE) or in heterotypic tissue recombinants between vaginal and bladder tissues (VS+BLE and BLS+VE). In BLS+VE recombinants the epithelium remained atrophic and failed to respond to exogenous oestrogen alone or in combination with progesterone. This lack of hormonal responsiveness of vaginal epithelium was completely reconstituted when the epithelium of BLS+VE recombinants was recovered and reassociated with fresh vaginal stroma (VS). Examination of epithelial proliferative activity ([3H]thymidine labelling index) demonstrated a marked oestrogen-induced increase in epithelial proliferation in VS+VE recombinants. BLS+BLE recombinants were unresponsive to oestrogen as were recombinants composed of BLS+VE. However, when bladder epithelium was grown in association with vaginal stroma (VS+BLE) the epithelium exhibited an 8-fold oestrogen-induced increase in labelling index over oil-treated specimens. The lack of an oestrogen-induced proliferative response of vaginal epithelium in BLS+VE recombinants was reversed when the vaginal epithelium of these recombinants was recovered and reassociated with fresh vaginal stroma. These results indicate that the effects of oestrogen and progesterone on both epithelial differentiation and proliferation are critically dependent upon the appropriate stromal environment.","PeriodicalId":77116,"journal":{"name":"Epithelial cell biology","volume":"1 1","pages":"18-31"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12481845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circadian variations in cell cycle phase progression of mouse epidermal cells measured directly by bivariate BrdUrd/DNA flow cytometry. 双变量BrdUrd/DNA流式细胞术直接测量小鼠表皮细胞周期阶段进展的昼夜节律变化。

Epithelial cell biology Pub Date : 1992-01-01

B Kirkhus, O P Clausen

引用次数: 0