Development (Cambridge, England). Supplement最新文献_第10页

The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila sensory organ precursors cells 在果蝇感觉器官前体细胞中，网格蛋白接头AP-1和Stratum通过平行通路控制Notch的激活

Development (Cambridge, England). Supplement Pub Date : 2020-01-01 DOI: 10.1101/2020.04.08.033092

K. Bellec, M. Pinot, I. Gicquel, R. Le Borgne

{"title":"The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila sensory organ precursors cells","authors":"K. Bellec, M. Pinot, I. Gicquel, R. Le Borgne","doi":"10.1101/2020.04.08.033092","DOIUrl":"https://doi.org/10.1101/2020.04.08.033092","url":null,"abstract":"ABSTRACT Drosophila sensory organ precursors divide asymmetrically to generate pIIa/pIIb cells, the identity of which relies on activation of Notch at cytokinesis. Although Notch is present apically and basally relative to the midbody at the pIIa-pIIb interface, the basal pool of Notch is reported to be the main contributor for Notch activation in the pIIa cell. Intra-lineage signalling requires appropriate apico-basal targeting of Notch, its ligand Delta and its trafficking partner Sanpodo. We have previously reported that AP-1 and Stratum regulate the trafficking of Notch and Sanpodo from the trans-Golgi network to the basolateral membrane. Loss of AP-1 or Stratum caused mild Notch gain-of-function phenotypes. Here, we report that their concomitant loss results in a penetrant Notch gain-of-function phenotype, indicating that they control parallel pathways. Although unequal partitioning of cell fate determinants and cell polarity were unaffected, we observed increased amounts of signalling-competent Notch as well as Delta and Sanpodo at the apical pIIa-pIIb interface, at the expense of the basal pool of Notch. We propose that AP-1 and Stratum operate in parallel pathways to localize Notch and control where receptor activation takes place. Summary: The Notch pathway activation relies on the correct localization of the Notch signalling actors. We report that AP-1 and Stratum ensure the basolateral targeting of Notch during asymmetric cell division.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77985167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Pineal progenitors originate from a non-neural territory limited by FGF signalling 松果体祖细胞起源于受FGF信号限制的非神经区域

Development (Cambridge, England). Supplement Pub Date : 2019-11-15 DOI: 10.1242/dev.171405

N. Staudt, F. Giger, Triona Fielding, J. A. Hutt, Isabelle Foucher, Victoria Snowden, Agathe Hellich, C. Kiecker, C. Houart

{"title":"Pineal progenitors originate from a non-neural territory limited by FGF signalling","authors":"N. Staudt, F. Giger, Triona Fielding, J. A. Hutt, Isabelle Foucher, Victoria Snowden, Agathe Hellich, C. Kiecker, C. Houart","doi":"10.1242/dev.171405","DOIUrl":"https://doi.org/10.1242/dev.171405","url":null,"abstract":"ABSTRACT The embryonic development of the pineal organ, a neuroendocrine gland on top of the diencephalon, remains enigmatic. Classic fate-mapping studies suggested that pineal progenitors originate from the lateral border of the anterior neural plate. We show here, using gene expression and fate mapping/lineage tracing in zebrafish, that pineal progenitors originate, at least in part, from the non-neural ectoderm. Gene expression in chick indicates that this non-neural origin of pineal progenitors is conserved in amniotes. Genetic repression of placodal, but not neural crest, cell fate results in pineal hypoplasia in zebrafish, while mis-expression of transcription factors known to specify placodal identity during gastrulation promotes the formation of ectopic pineal progenitors. We also demonstrate that fibroblast growth factors (FGFs) position the pineal progenitor domain within the non-neural border by repressing pineal fate and that the Otx transcription factors promote pinealogenesis by inhibiting this FGF activity. The non-neural origin of the pineal organ reveals an underlying similarity in the formation of the pineal and pituitary glands, and suggests that all CNS neuroendocrine organs may require a non-neural contribution to form neurosecretory cells. Highlighted Article: Gene expression and fate mapping/lineage tracing in zebrafish reveals that the pineal organ develops from the non-neural pre-placodal ectoderm under the control of FGF signalling.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78716536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Crosslinking activity of non-muscle myosin II is not sufficient for embryonic cytokinesis in C. elegans 非肌球蛋白II的交联活性不足以进行秀丽隐杆线虫的胚胎细胞分裂

Development (Cambridge, England). Supplement Pub Date : 2019-11-01 DOI: 10.1242/dev.179150

D. S. Osório, Fung-Yi Chan, J. Saramago, J. Leite, A. M. Silva, A. F. Sobral, R. Gassmann, A. Carvalho

{"title":"Crosslinking activity of non-muscle myosin II is not sufficient for embryonic cytokinesis in C. elegans","authors":"D. S. Osório, Fung-Yi Chan, J. Saramago, J. Leite, A. M. Silva, A. F. Sobral, R. Gassmann, A. Carvalho","doi":"10.1242/dev.179150","DOIUrl":"https://doi.org/10.1242/dev.179150","url":null,"abstract":"ABSTRACT Cytokinesis in animal cells requires the assembly and constriction of a contractile actomyosin ring. Non-muscle myosin II is essential for cytokinesis, but the role of its motor activity remains unclear. Here, we examine cytokinesis in C. elegans embryos expressing non-muscle myosin motor mutants generated by genome editing. Two non-muscle motor-dead myosins capable of binding F-actin do not support cytokinesis in the one-cell embryo, and two partially motor-impaired myosins delay cytokinesis and render rings more sensitive to reduced myosin levels. Further analysis of myosin mutants suggests that it is myosin motor activity, and not the ability of myosin to crosslink F-actin, that drives the alignment and compaction of F-actin bundles during contractile ring assembly, and that myosin motor activity sets the pace of contractile ring constriction. We conclude that myosin motor activity is required at all stages of cytokinesis. Finally, characterization of the corresponding motor mutations in C. elegans major muscle myosin shows that motor activity is required for muscle contraction but is dispensable for F-actin organization in adult muscles. This article has an associated ‘The people behind the papers’ interview. Highlighted Article: The motor activity of non-muscle myosin II is essential for cytokinesis and contributes to all stages of the process in C. elegans embryos.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79314336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Principles and applications of optogenetics in developmental biology 光遗传学原理及其在发育生物学中的应用

Development (Cambridge, England). Supplement Pub Date : 2019-10-15 DOI: 10.1242/dev.175067

Daniel Krueger, Emiliano Izquierdo, Ranjith Viswanathan, Jonas Hartmann, Cristina Pallares Cartes, S. De Renzis

引用次数: 73

Spatiotemporal regulation of multipotency during prostate development 前列腺发育过程中多能性的时空调控

Development (Cambridge, England). Supplement Pub Date : 2019-10-15 DOI: 10.1242/dev.180224

Elisavet Tika, Marielle Ousset, Anne Dannau, C. Blanpain

{"title":"Spatiotemporal regulation of multipotency during prostate development","authors":"Elisavet Tika, Marielle Ousset, Anne Dannau, C. Blanpain","doi":"10.1242/dev.180224","DOIUrl":"https://doi.org/10.1242/dev.180224","url":null,"abstract":"ABSTRACT The prostate is formed by a branched glandular epithelium composed of basal cells (BCs) and luminal cells (LCs). Multipotent and unipotent stem cells (SCs) mediate the initial steps of prostate development whereas BCs and LCs are self-sustained in adult mice by unipotent lineage-restricted SCs. The spatiotemporal regulation of SC fate and the switch from multipotency to unipotency remain poorly characterised. Here, by combining lineage tracing, whole-tissue imaging, clonal analysis and proliferation kinetics, we uncover the cellular dynamics that orchestrate prostate postnatal development in mouse. We found that at an early stage of development multipotent basal SCs are located throughout the epithelium and are progressively restricted at the distal tip of the ducts, where, together with their progeny, they establish the different branches and the final structure of prostate. In contrast, pubertal development is mediated by unipotent lineage-restricted SCs. Our results uncover the spatiotemporal regulation of the switch from multipotency to unipotency during prostate development. Highlighted Article: A combination of lineage tracing and whole-mount imaging uncovers how the multipotency of basal stem cells is regulated during postnatal prostate development in mouse.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75275800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis 活细胞分析显示，在斑马鱼合子基因组激活过程中，组蛋白H3K27乙酰化先于活性转录

Development (Cambridge, England). Supplement Pub Date : 2019-09-30 DOI: 10.1242/dev.179127

Y. Sato, Lennart Hilbert, H. Oda, Yinan Wan, John M. Heddleston, T. Chew, V. Zaburdaev, Philipp J. Keller, T. Lionnet, N. Vastenhouw, H. Kimura

{"title":"Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis","authors":"Y. Sato, Lennart Hilbert, H. Oda, Yinan Wan, John M. Heddleston, T. Chew, V. Zaburdaev, Philipp J. Keller, T. Lionnet, N. Vastenhouw, H. Kimura","doi":"10.1242/dev.179127","DOIUrl":"https://doi.org/10.1242/dev.179127","url":null,"abstract":"ABSTRACT Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms. Summary: FabLEM, an endogenous labeling technique that uses modification-specific antigen-binding fragments, is used to examine changes in histone modification levels and transcription during zygotic genome activation in live zebrafish embryos.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"134 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76871283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

The Hox gene Abdominal-B uses DoublesexF as a cofactor to promote neuroblast apoptosis in the Drosophila central nervous system Hox基因Abdominal-B使用doubesexf作为辅助因子促进果蝇中枢神经系统的神经母细胞凋亡

Development (Cambridge, England). Supplement Pub Date : 2019-08-15 DOI: 10.1242/dev.175158

Neha Ghosh, Asif Bakshi, Risha Khandelwal, Sriivatsan G. Rajan, R. Joshi

{"title":"The Hox gene Abdominal-B uses DoublesexF as a cofactor to promote neuroblast apoptosis in the Drosophila central nervous system","authors":"Neha Ghosh, Asif Bakshi, Risha Khandelwal, Sriivatsan G. Rajan, R. Joshi","doi":"10.1242/dev.175158","DOIUrl":"https://doi.org/10.1242/dev.175158","url":null,"abstract":"ABSTRACT Highly conserved DM domain-containing transcription factors (Doublesex/MAB-3/DMRT1) are responsible for generating sexually dimorphic features. In the Drosophila central nervous system, a set of Doublesex (Dsx)-expressing neuroblasts undergo apoptosis in females whereas their male counterparts proliferate and give rise to serotonergic neurons crucial for adult mating behaviour. Our study demonstrates that the female-specific isoform of Dsx collaborates with Hox gene Abdominal-B (Abd-B) to bring about this apoptosis. Biochemical results suggest that proteins AbdB and Dsx interact through their highly conserved homeodomain and DM domain, respectively. This interaction is translated into a cooperative binding of the two proteins on the apoptotic enhancer in the case of females but not in the case of males, resulting in female-specific activation of apoptotic genes. The capacity of AbdB to use the sex-specific isoform of Dsx as a cofactor underlines the possibility that these two classes of protein are capable of cooperating in selection and regulation of target genes in a tissue- and sex-specific manner. We propose that this interaction could be a common theme in generating sexual dimorphism in different tissues across different species. Highlighted Article: Drosophila DoublesexF collaborates with Abdominal-B to generate a sexually dimorphic central nervous system.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82844670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Precision of tissue patterning is controlled by dynamical properties of gene regulatory networks 基因调控网络的动态特性控制着组织模式的精度

Development (Cambridge, England). Supplement Pub Date : 2019-07-31 DOI: 10.1101/721043

Katherine Exelby, Edgar Herrera-Delgado, Lorena Garcia Perez, R. Pérez-Carrasco, A. Sagner, Vicki Metzis, Peter Sollich, J. Briscoe

{"title":"Precision of tissue patterning is controlled by dynamical properties of gene regulatory networks","authors":"Katherine Exelby, Edgar Herrera-Delgado, Lorena Garcia Perez, R. Pérez-Carrasco, A. Sagner, Vicki Metzis, Peter Sollich, J. Briscoe","doi":"10.1101/721043","DOIUrl":"https://doi.org/10.1101/721043","url":null,"abstract":"ABSTRACT During development, gene regulatory networks allocate cell fates by partitioning tissues into spatially organised domains of gene expression. How the sharp boundaries that delineate these gene expression patterns arise, despite the stochasticity associated with gene regulation, is poorly understood. We show, in the vertebrate neural tube, using perturbations of coding and regulatory regions, that the structure of the regulatory network contributes to boundary precision. This is achieved, not by reducing noise in individual genes, but by the configuration of the network modulating the ability of stochastic fluctuations to initiate gene expression changes. We use a computational screen to identify network properties that influence boundary precision, revealing two dynamical mechanisms by which small gene circuits attenuate the effect of noise in order to increase patterning precision. These results highlight design principles of gene regulatory networks that produce precise patterns of gene expression. Summary: Experiments and modeling reveal sharp boundaries of gene expression in the vertebrate neural tube depend on the dynamics of the gene regulatory network that patterns the tissue.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81217600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Neural tube development depends on notochord-derived sonic hedgehog released into the sclerotome 神经管的发育依赖于脊索衍生的超音hedgehog基因释放到硬核组

Development (Cambridge, England). Supplement Pub Date : 2019-05-15 DOI: 10.1101/639831

N. Kahane, Chaya Kalcheim

{"title":"Neural tube development depends on notochord-derived sonic hedgehog released into the sclerotome","authors":"N. Kahane, Chaya Kalcheim","doi":"10.1101/639831","DOIUrl":"https://doi.org/10.1101/639831","url":null,"abstract":"ABSTRACT Sonic hedgehog (Shh), produced in the notochord and floor plate, is necessary for both neural and mesodermal development. To reach the myotome, Shh has to traverse the sclerotome and a reduction of sclerotomal Shh affects myotome differentiation. By investigating loss and gain of Shh function, and floor-plate deletions, we report that sclerotomal Shh is also necessary for neural tube development. Reducing the amount of Shh in the sclerotome using a membrane-tethered hedgehog-interacting protein or Patched1, but not dominant active Patched, decreased the number of Olig2+ motoneuron progenitors and Hb9+ motoneurons without a significant effect on cell survival or proliferation. These effects were a specific and direct consequence of Shh reduction in the mesoderm. In addition, grafting notochords in a basal but not apical location, vis-à-vis the tube, profoundly affected motoneuron development, suggesting that initial ligand presentation occurs at the basal side of epithelia corresponding to the sclerotome-neural tube interface. Collectively, our results reveal that the sclerotome is a potential site of a Shh gradient that coordinates the development of mesodermal and neural progenitors. Summary: Loss- and gain-of-function, and floor plate deletions, reveal that Shh that transits through the sclerotome is presented to the neuroepithelium from its basal aspect to affect motoneuron development.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80482679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis FGF信号在蛇尾丝状Amphiura filiformis骨骼的发育和再生中起着类似的作用

Development (Cambridge, England). Supplement Pub Date : 2019-05-09 DOI: 10.1101/632968

A. Czarkwiani, D. Dylus, L. Carballo, P. Oliveri

{"title":"FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis","authors":"A. Czarkwiani, D. Dylus, L. Carballo, P. Oliveri","doi":"10.1101/632968","DOIUrl":"https://doi.org/10.1101/632968","url":null,"abstract":"Regeneration is an adult developmental process considered to be an epiphenomenon of embryonic development. Although several studies have shown that various embryonic genes are expressed during regeneration, there have been no large-scale, direct and functional comparative studies between the development and regeneration of a specific structure in one animal. Here, we use the brittle star Amphiura filiformis to characterise the role of the FGF signalling pathway during skeletal development and regeneration. In both processes, we find the ligands expressed in ectodermal cells flanking underlying mesodermal cells, and the receptors expressed specifically by these skeletogenic cells. Perturbation of FGF but not VEGF signalling during skeletogenesis completely inhibited skeleton formation in both embryogenesis and regeneration, without affecting other key developmental processes like cell migration or proliferation. Transcriptome-wide differential analysis identified a highly similar cohort of skeletogenic differentiation genes downstream of the FGF signalling pathway, whereas upstream transcription factors involved in the initial specification of the skeletogenic lineage where unaffected. Comparison to the sea urchin indicated that many of the affected genes are associated with differentiation. Moreover, several genes showed no homology to a cohort from other species, leading to the discovery of brittle star specific, downstream skeletogenic genes. In conclusion, our results show that the FGF pathway is crucial for skeletogenesis in the brittle star, as it is in other deuterostomes, and for the first time provide evidence for the re-deployment of a gene regulatory module during both regeneration and development.","PeriodicalId":77105,"journal":{"name":"Development (Cambridge, England). Supplement","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80886699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20