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Australian journal of biotechnology最新文献

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A TAFE strategy for biotechnology skills training. TAFE生物技术技能培训战略。
Australian journal of biotechnology Pub Date : 1990-01-01
R Stark
{"title":"A TAFE strategy for biotechnology skills training.","authors":"R Stark","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The outline of a strategy for training technicians who will undertake biotechnology-oriented tasks as employees is the focus of this review. Technical and Further Education (TAFE) in Western Australia has recently restructured electives offered in certificate and diploma courses of Applied Science. The aim of restructuring was to allow a broader choice catering for specialized streams. With a few additions it would be possible to list a set of biotechnology options for technicians specializing in this area. After identifying employers in Western Australia, data on technician employment characteristics and training needs was gathered through a survey and used to formulate a future development strategy. In the past little attention has been given anywhere in Australia to the training needs of this group. The availability of such appropriately trained employees will increasingly be recognized as a vital link in the success of any biotechnology enterprise.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"4 1","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12538523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Animal health and production in the 21st Century. A conference held at Sydney University, 9-10 November 1989. 21世纪的动物卫生和生产。1989年11月9日至10日在悉尼大学举行的会议。
Australian journal of biotechnology Pub Date : 1990-01-01
V Sarma
{"title":"Animal health and production in the 21st Century. A conference held at Sydney University, 9-10 November 1989.","authors":"V Sarma","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"4 1","pages":"15, 17"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12541312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Environmental issues in the planned release of genetically-engineered organisms. 基因工程生物计划释放中的环境问题。
Australian journal of biotechnology Pub Date : 1989-01-01
V Sarma
{"title":"Environmental issues in the planned release of genetically-engineered organisms.","authors":"V Sarma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper examines issues concerned with the environmental release of genetically-engineered micro-organisms. Besides the obvious social and economic benefits from the technology, genetically-engineered micro-organisms can have considerable beneficial effects on environmental concerns, such as the degradation of pollutants and toxic chemical wastes, and less use of hazardous pesticides and chemicals. There may be uncertain negative effects arising from the technology. The hazards of the technology and possible policy approaches are discussed. It is concluded that, overall, the applications arising from this technology are likely to be benign, because its effects on the environment can largely be anticipated by an increase in scientific knowledge gained from field trials and greater experience with releases.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"13-6"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using chromatography in downstream processing. 在下游加工中使用色谱法。
Australian journal of biotechnology Pub Date : 1989-01-01
C Becker
{"title":"Using chromatography in downstream processing.","authors":"C Becker","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This article concludes the series on the use of chromatography for downstream processing. Although it has only scratched the surface when considering the number of parameters involved in process chromatography, it does give a broad overview including the choice of components through process standards. Pharmacia LKB Biotechnology has had more than 15 years experience in the design development and running of large scale chromatographic processes. During this time the company has gathered a vast amount of experience and information on the key points to successful product purification. Pharmacia LKB can advise on the choice of techniques and the development of a separation process up to full production scale.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"18-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Megabase DNA electrophoresis--recent advances. 百万碱基DNA电泳——最新进展。
Australian journal of biotechnology Pub Date : 1989-01-01
S Ferris, L Sparrow, A Stevens
{"title":"Megabase DNA electrophoresis--recent advances.","authors":"S Ferris,&nbsp;L Sparrow,&nbsp;A Stevens","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"33-5"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The formation of biologically active beta-galactosidase inclusion bodies in Escherichia coli. 生物活性β -半乳糖苷酶包涵体在大肠杆菌中的形成。
Australian journal of biotechnology Pub Date : 1989-01-01
D M Worrall, N H Goss
{"title":"The formation of biologically active beta-galactosidase inclusion bodies in Escherichia coli.","authors":"D M Worrall,&nbsp;N H Goss","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Culture conditions affecting the formation of beta-galactosidase inclusion bodies in E. coli were examined. High temperature, early induction, high salt concentration and low aeration were all found to favour an increase of insoluble beta-galactosidase and the formation of visible inclusion bodies. The ratio of soluble to insoluble beta-galactosidase decreased during the course of cell growth. When assayed for beta-galactosidase activity, the inclusion bodies were enzymatically active with a specific activity of one third that of soluble beta-galactosidase. The activity remained associated with the inclusion bodies on washing with detergent and high ionic strength buffers. These results suggest that inclusion bodies can contain correctly folded protein.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"28-32"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enterovax breakthrough in cholera vaccine trial. 霍乱疫苗试验取得突破性进展。
Australian journal of biotechnology Pub Date : 1989-01-01
{"title":"Enterovax breakthrough in cholera vaccine trial.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"27"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biotechnology information sources. 生物技术信息源。
Australian journal of biotechnology Pub Date : 1989-01-01
{"title":"Biotechnology information sources.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"40"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kinetic properties and contribution to cellulose saccharification of a cloned Pseudomonas beta-glucosidase. 克隆假单胞菌-葡萄糖苷酶的动力学性质及其对纤维素糖化的贡献。
Australian journal of biotechnology Pub Date : 1989-01-01
P A Rickard, B A Ghani, R J Lucas, N W Dunn
{"title":"Kinetic properties and contribution to cellulose saccharification of a cloned Pseudomonas beta-glucosidase.","authors":"P A Rickard,&nbsp;B A Ghani,&nbsp;R J Lucas,&nbsp;N W Dunn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The plasmid pND71, which encodes beta-glucosidase (cellobiase) activity, cloned from the cellulolytic Pseudomonad, PS2-2, was mobilized by conjugation into 10 Pseudomonas strains. The highest specific activity was produced by 17498 (pND71) and the properties of the enzyme produced from this transconjugant were studied. The enzyme was shown to be cell associated, to have a temperature optimum of 37 degrees C, a pH optimum of 7.0 and Km values of 1.33 and 2.94 mM for pNPG and cellobiose respectively. It was competitively inhibited by glucose, with a Ki of 30 mM. Evidence was obtained which suggested that the enzyme was produced constitutively and that synthesis was not repressed by glucose. When culture preparations were used in combination with Trichoderma reesei QM9414 and C30 enzyme preparations to saccharify cellulose, 17498 (pND71) was more effective than preparations of PS2-2 in acting synergistically with T. reesei to solubilize more carbohydrate and produce more glucose.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"43-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Downstream processing of monoclonal antibodies. 单克隆抗体的下游加工。
Australian journal of biotechnology Pub Date : 1989-01-01
J M Gunnersen, L F Fowles, J M Snelgar, C A Mitchell, J V Patava, K J Osborne, S M Mahler
{"title":"Downstream processing of monoclonal antibodies.","authors":"J M Gunnersen,&nbsp;L F Fowles,&nbsp;J M Snelgar,&nbsp;C A Mitchell,&nbsp;J V Patava,&nbsp;K J Osborne,&nbsp;S M Mahler","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"3 1","pages":"69-71"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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