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Australian journal of biotechnology最新文献

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Genetic manipulation enquiry--the way ahead. 基因操纵调查，未来的方向。
Australian journal of biotechnology Pub Date : 1991-04-01
M J Sleigh
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引用次数: 0
A single step method for the solubilization and refolding of recombinant protein from E. coli inclusion bodies. 一种从大肠杆菌包涵体中提取重组蛋白的增溶和再折叠的单步方法。
Australian journal of biotechnology Pub Date : 1991-04-01
E Crivelli, M Cardamone, N K Puri
{"title":"A single step method for the solubilization and refolding of recombinant protein from E. coli inclusion bodies.","authors":"E Crivelli,&nbsp;M Cardamone,&nbsp;N K Puri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Expression of recombinant porcine growth hormone (rpGH) in E. coli cells resulted in the accumulation of the rpGH within inclusion bodies (IBs). The IBs were solubilized and the rpGH refolded in a single step using a cationic surfactant, N-cetyl pyridinium chloride (CPC; C21H38ClN) in the absence of reducing agents. No additional dialysis or rapid dilution steps of the solubilizing agent were required to obtain a 30% yield of refolded and oxidized rpGH monomer at protein concentrations of up to 15-20 mg/mL. In contrast, the refolding in vitro of rpGH in the absence of CPC resulted in the formation of significant amounts of higher molecular weight aggregate at the expense of the biologically active monomer. The fluorescence spectrum of the purified refolded rpGH was indistinguishable from that of biologically active, pituitary derived porcine GH and reverse-phase HPLC analysis of the purified rpGH showed similar retention times to that of pituitary GH. It is likely that the use of cetylpyridinium chloride is generally applicable to the simplified high yield recovery of biologically active recombinant proteins from IBs.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"78-80, 86"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel ion-exchangers for the chromatographic purification of biopolymers--chemistry and column technology. 用于生物聚合物色谱纯化的新型离子交换剂——化学与柱技术。
Australian journal of biotechnology Pub Date : 1991-04-01
R Ditz, G Hauke, W Muller
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引用次数: 0
Analytical biotechnology: applications for downstream processing. 分析生物技术:下游加工的应用。
Australian journal of biotechnology Pub Date : 1991-04-01
K L Williams, A A Gooley, P A Haynes, M Batley, J H Curtin, M C Stuart, A C Champion, D D Sheumack, J W Redmond
{"title":"Analytical biotechnology: applications for downstream processing.","authors":"K L Williams,&nbsp;A A Gooley,&nbsp;P A Haynes,&nbsp;M Batley,&nbsp;J H Curtin,&nbsp;M C Stuart,&nbsp;A C Champion,&nbsp;D D Sheumack,&nbsp;J W Redmond","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"96-100"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Continuous flow centrifugation. 连续流离心。
Australian journal of biotechnology Pub Date : 1991-04-01 DOI: 10.1002/9783527678679.dg02387
G. Burge
{"title":"Continuous flow centrifugation.","authors":"G. Burge","doi":"10.1002/9783527678679.dg02387","DOIUrl":"https://doi.org/10.1002/9783527678679.dg02387","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"19 2","pages":"93-5"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50802487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Recovery and purification of polysaccharides from microbial broth. 微生物发酵液中多糖的回收纯化。
Australian journal of biotechnology Pub Date : 1991-04-01
M R Johns, E Noor
{"title":"Recovery and purification of polysaccharides from microbial broth.","authors":"M R Johns,&nbsp;E Noor","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Current industrial practice to recover extracellular microbial polysaccharides from the broth usually requires dilution to permit cell removal followed by precipitation, typically using alcohol. This paper presents a discussion on the solvent precipitation of xanthan and the results of research performed to investigate the behaviour of xanthan solutions during membrane processing using a microporous membrane. Using crossflow microfiltration, flux rates of up to 120 L/m2h were achieved for pure xanthan solutions, with complete rejection of the polysaccharide by the membrane. The thin film model underpredicted flux for xanthan solutions. In fact, flux was independent of xanthan concentration up to 20-25 g/L, and strongly dependent on crossflow velocity. Considerable benefits in terms of purification and reduced solvent requirements can be obtained by the use of an intermediate crossflow microfiltration step during xanthan recovery.</p>","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"73-7"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viral contamination of therapeutic proteins: a challenge for downstream processing. 治疗性蛋白的病毒污染:对下游加工的挑战。
Australian journal of biotechnology Pub Date : 1991-04-01
C Harbour, G Woodhouse, J P Barford, J Spencer, A J MacLeod
{"title":"Viral contamination of therapeutic proteins: a challenge for downstream processing.","authors":"C Harbour,&nbsp;G Woodhouse,&nbsp;J P Barford,&nbsp;J Spencer,&nbsp;A J MacLeod","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"81-6"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Opportunities in New Zealand for biotechnology process development and contract research. The Biotechnology Group of DSIR Industrial Development, New Zealand. 新西兰生物技术工艺开发和合同研究的机会。新西兰DSIR工业发展的生物技术小组。
Australian journal of biotechnology Pub Date : 1991-04-01
M J Kennedy, J Davies
{"title":"Opportunities in New Zealand for biotechnology process development and contract research. The Biotechnology Group of DSIR Industrial Development, New Zealand.","authors":"M J Kennedy,&nbsp;J Davies","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Continuous flow centrifugation. 连续流离心。
Australian journal of biotechnology Pub Date : 1991-04-01
G Burge
{"title":"Continuous flow centrifugation.","authors":"G Burge","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"93-5"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The commercialization of biotechnology: important aspects of technology licensing. 生物技术的商业化:技术许可的重要方面。
Australian journal of biotechnology Pub Date : 1991-04-01
S Irvine
{"title":"The commercialization of biotechnology: important aspects of technology licensing.","authors":"S Irvine","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77022,"journal":{"name":"Australian journal of biotechnology","volume":"5 2","pages":"105-8"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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