Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology最新文献

{"title":"Goodbye Analytical Cellular Pathology, Hello Cellular Oncology!","authors":"Van Diest, J. Paul","doi":"10.1155/2003/379168","DOIUrl":"https://doi.org/10.1155/2003/379168","url":null,"abstract":"","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 1","pages":"209-209"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/379168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64134066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Presentations","authors":"S. Briggs, J. Finch, Aayesha Mulla, Lorraine Ainscow, Hannah Linford","doi":"10.1155/2003/473736","DOIUrl":"https://doi.org/10.1155/2003/473736","url":null,"abstract":"Background and Aim: Neoplastic cells need essential metals such as iron and copper for cellular functions and rapid growth. In breast cancer cells, human epidermal growth factor receptor 2 (HER2) is overexpressed around 30% with poor prognosis and this results in elevated the proportion of cancer stem cells (CSCs) that are in charge of cancer recurrence. Metal chelation and changing their redox cycle in favor of oxidative stress may be a critical to make these cells vulnerable to cell death. Investigating whether metal chelation alters HER2-induced CSC population may provide a new tools for breast cancer therapy. Material and Methods: MCF7-HER2, overexpressing HER2, and MCF7-vec control cells were used to evaluate the effect of HER2. Also, we have used other breast cancer cell lines; HCC1954, MDA-MB-435 and Hs578T in order to substantiate our results. DFO and Dp44mT were used as metal chelators. ROS production, iron levels and CSC survival in response to chelators were detected by flow cytometry and cell viability was measured by MTT assay. Results: MCF7-HER2 cells require iron more than their vector counterparts and HER2-increased CSCs are vulnerable to iron chelation. Additionally, this sensitivity of CSCs to iron reduction is obviously indicated in other breast cancer cell lines. Finally, the concept also in neoplastically transformed breast cancer cell line, HMLER. ROS were relatively increased by Dp44mT in the cells this was reversed by combination of iron while copper combination further induced ROS. Parallel changes were observed in the inhibition of cell growth by Dp44mT and this was partially rescued by NAC supplement. Discussion Conclusion: study cancer cells fluorescence which covers pathogenic or beneficial Mc. We focused on do Mc play any role in carcinogenesis. Neuroblastoma (NB) is the most common extracranial solid cancer in childhood. Cancer stem cells (CSCs) are thought to be associated with micrometastasis, cause of cancer,drug resistance and recurrences. Recent studies showed that sanguinarine (Sng) could used as an anti-cancer due to its apoptosis inducing mechanism. FBS is used as a common supplement in most of the in vitro studies however there are other factors interacting with tumor cells and CSCs in in vivo conditions. Thus, the aim of this study was to evaluate the effect of Sng on NB CSCs in different culture conditious. patients with breast cancer is not satisfactory. In recent years, this failure attributed to cancer stem cells (CSCs) as they show and/or gain resistance to therapies. Thus, compounds that target CSCs are urgently needed. The aim of this study is to investigate the cytotoxic activity of tingenin b (or 22β-hydroxytingenone, a quinone-methide triterpenoid structurally related to tingenone) in combination with paclitaxel against breast CSCs. Material&Methods: The anti-growth activity was investigated by the ATP assay. Mode of cell death was evaluated using fluorescence microscopy (Hoechst 33342+Propidium ","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"248 1","pages":"217 - 247"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75043334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA extraction from bronchial aspirates for molecular cytology: which method to take?","authors":"Hans Jürgen Grote,&nbsp;Viola Schmiemann,&nbsp;Mario Sarbia,&nbsp;Alfred Böcking","doi":"10.1155/2003/354796","DOIUrl":"https://doi.org/10.1155/2003/354796","url":null,"abstract":"<p><strong>Objective: </strong>To date, there are only few systematic reports on the quality of DNA extracted from routine diagnostic cytologic specimens. It was the aim of the present study to evaluate the ability of 50% ethanol/2% carbowax (Saccomanno fixative) to preserve bronchial secretions with high quality genomic DNA as well as to compare different DNA extraction methods.</p><p><strong>Methods: </strong>DNA was extracted from 45 bronchial aspirates by four different extraction protocols. Beside DNA yield, DNA quality with regard to purity, integrity, and PCR success rate were investigated.</p><p><strong>Results: </strong>No fragmentation of sample DNA due to the fixative was detected. It was preserved as high molecular weight DNA. DNA yield, purity, and integrity were dependent on the DNA extraction method to some extend. Irrespective of the DNA extraction method the PCR success rate for amplification of beta-globin gene fragments (268, 536, and 989 bp) was 100%.</p><p><strong>Conclusion: </strong>A fixative containing 50% ethanol/2% carbowax preserves high quality DNA which is well suited for PCR-based assays regardless of the extraction protocol used. The selection of the DNA extraction protocol has to be adjusted to the circumstances of application.</p>","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 2","pages":"83-8"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/354796","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22286305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of FISH-procedures: summary of the first discussion workshop.","authors":"Michael Hausmann,&nbsp;Christoph Cremer","doi":"10.1155/2003/427509","DOIUrl":"https://doi.org/10.1155/2003/427509","url":null,"abstract":"Currently, fluorescent in situ hybridization (FISH) is widely used to assess the localization of genetic elements in tissues, nuclei of cultured cells, and spreads of metaphase chromosomes. The advent of commercially available probes and labelling kits for whole or partial chromosome painting and pathogenetically important gene loci has allowed FISH to enter routine work. Still, however, FISH procedures vary grossly from laboratory to laboratory, and are far from optimal for many questions in modern biology and medicine. Here, a report on the 1st discussion workshop on standardization of FISH-procedures held at Schloss Elmau, Bavaria, October 9–10, 2002 is given. This meeting was organised by Christoph Cremer (Heidelberg), Michael Hausmann (Freiburg) and Hans J. Tanke (Leiden) as a satellite workshop to the 2nd Elmau conference on nuclear organization. The workshop was convented to discuss recent developments, problems of routine applications, and future requirements in the intriguing subject of specific fluorescence DNA labelling especially in the interphase cell nucleus. The idea was to bring together applicants of diagnostic routine, applicants in basic cytogenetic research, and developers of FISH techniques. The 10 participants very lively supported the discussion and elaborated some future aspects for methodological research and requirements to FISH probe manufacturers.","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 4","pages":"201-5"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/427509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40894312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of quantitative pathology in clinical decision making for Barrett's oesophagus.","authors":"Wojciech Polkowski,&nbsp;J Jan B van Lanschot,&nbsp;Johanna W van Sandick,&nbsp;Jan P A Baak","doi":"10.1155/2003/496828","DOIUrl":"https://doi.org/10.1155/2003/496828","url":null,"abstract":"Intestinal-type columnar epithelium in the (distal) oesophagus, known as Barrett’s oesophagus (BO), is a well-defined premalignant condition [32]. The risk for the development of oesophageal adenocarcinoma in a patient with BO is at least 30 times higher as compared to the general population [9]. Invasive cancer in BO, so called Barrett cancer, is preceded by stages of progressively severe dysplastic changes [24]. For a symptomatic Barrett cancer, long-term survival rates are low. Therefore, attention should be focused on early detection of neoplastic changes, preferably in a preinvasive phase, i.e. dysplasia. An accurate and reproducible diagnosis of dysplasia in BO might ultimately lead to targeted therapeutic interventions or cancer prevention in the future. At present, dysplasia in BO is the only clinically accepted marker of neoplastic potential. Strategies for endoscopic surveillance of BO are dictated by the grade of dysplasia on endoscopic biopsy [17,23]. When a diagnosis of low-grade dysplasia is made, surveillance should be intensified by shortening the time intervals between consecutive endoscopies and by applying more aggressive biopsy sampling [35,36]. High-grade dysplasia may indicate imminent progression into invasive carcinoma or even its occult presence [1,5,10,18,27,30]. When the diagnosis of (persis-","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 3","pages":"123-7"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/496828","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22409635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A feature set for cytometry on digitized microscopic images.","authors":"Karsten Rodenacker,&nbsp;Ewert Bengtsson","doi":"10.1155/2003/548678","DOIUrl":"https://doi.org/10.1155/2003/548678","url":null,"abstract":"<p><p>Feature extraction is a crucial step in most cytometry studies. In this paper a systematic approach to feature extraction is presented. The feature sets that have been developed and used for quantitative cytology at the Laboratory for Biomedical Image Analysis of the GSF as well as at the Center for Image Analysis in Uppsala over the last 25 years are described and illustrated. The feature sets described are divided into morphometric, densitometric, textural and structural features. The latter group is used to describe the eu- and hetero-chromatin in a way complementing the textural methods. The main goal of the paper is to bring attention to the need of a common and well defined description of features used in cyto- and histometrical studies. The application of the sets of features is shown in an overview of projects from different fields. Finally some rules of thumb for the design of studies in this field are proposed. Colour figures can be viewed on http://www.esacp.org/acp/2003/25-1/rodenacker.htm.</p>","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 1","pages":"1-36"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/548678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22249406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of the cell adhesion molecule CD146/MCAM in non-small cell lung cancer.","authors":"Glen Kristiansen,&nbsp;Yongwei Yu,&nbsp;Karsten Schlüns,&nbsp;Christine Sers,&nbsp;Manfred Dietel,&nbsp;Iver Petersen","doi":"10.1155/2003/574829","DOIUrl":"https://doi.org/10.1155/2003/574829","url":null,"abstract":"<p><p>Expression of MCAM is observed in a variety of human malignancies. We aimed to determine the rate of MCAM expression in our non-small cell lung cancer (NSCLC) collection and to clarify its correlation with clinicopathological parameters and patient survival. 85 NSCLC were analysed immunohistochemically using a monoclonal MCAM antibody (clone N1238) on an NSCLC tissue micro array. The staining was semiquantitatively scored. We found MCAM expression in 51% of NSCLC, preferentially squamous cell carcinomas (p=0.004). No other correlations to tumour size, grade, or stage were found. Univariate survival analysis showed no significant differences of MCAM positive and negative tumours. In adenocarcinomas however, MCAM positivity was significantly associated with shorter patient survival (p=0.016). We conclude, that MCAM is expressed in a high proportion of NSCLC and might be predictive of shortened patient survival in adenocarcinomas of the lung. Colour figure can be viewed on http://www.esacp.org/acp/2003/25-2/kristiansen.htm</p>","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 2","pages":"77-81"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/574829","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22286304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenetic evolution of human ovarian cell lines associated with chemoresistance and loss of tumorigenicity.","authors":"Stéphanie Struski,&nbsp;Martine Doco-Fenzy,&nbsp;Michael Koehler,&nbsp;Ilse Chudoba,&nbsp;Francis Levy,&nbsp;Linda Masson,&nbsp;Nicole Michel,&nbsp;Evelyne Ulrich,&nbsp;Nadine Gruson,&nbsp;Jean Bénard,&nbsp;Gérard Potron,&nbsp;Pascale Cornillet-Lefebvre","doi":"10.1155/2003/151042","DOIUrl":"https://doi.org/10.1155/2003/151042","url":null,"abstract":"<p><p>In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex-FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug-resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)).</p>","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 3","pages":"115-22"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/151042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22409634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of grade of urothelial cell carcinomas and DNA histogram features assessed by flow cytometry and automated image cytometry.","authors":"Marco G W Bol,&nbsp;Jan P A Baak,&nbsp;Bianca Diermen,&nbsp;E A M Janssen,&nbsp;Susanne B K Buhr-Wildhagen,&nbsp;Kjell-Henning Kjellevold","doi":"10.1155/2003/410239","DOIUrl":"https://doi.org/10.1155/2003/410239","url":null,"abstract":"<p><strong>Objective: </strong>To analyse how DNA ploidy and S-phase fraction (SPF) by flow cytometry (FCM) and an optimised fully automatic DNA image cytometer (ICM) correlate with grade in TaT1 urothelial cell carcinomas (UC) of the urinary bladder.</p><p><strong>Materials and methods: </strong>Two-hundred-and twenty-eight consensus cases were analysed. Single cell suspensions were stained (DAPI for FCM, Feulgen for ICM). There was enough material for both FCM and ICM in 202 of these cases. FCM and optimised ICM measurements were performed on the 202 UCs. To discriminate between different grades, single- and multivariate analyses was performed on DNA histogram features obtained with the MultiCycle program (using DNA index (DI) and SPF).</p><p><strong>Results: </strong>Overall measurement time of the adapted ICM method was 10.7 minutes per case (range 5.9-29.8 min.) and required little additional interactive object rejection (average 152 objects (84-298) on 3000 objects per case measured, which took 9.9 minutes on average, range 8.3-15.5 minutes). The ICM histograms looked much \"cleaner\" with less noise than the FCM graphs. The coefficient of variation (CV) of the diploid peak for ICM (5.4%) was significantly lower than for FCM (5.9%) (p<0.0001). ICM features were more strongly correlated to grade than FCM features. In multivariate analysis, the best discriminating set of features was DNA ploidy and SPF (both by ICM).</p><p><strong>Conclusions: </strong>The adapted fully automated DNA ICM works very well for UCs. Low CV DNA ICM histograms are obtained in a time comparable to FCM. The DNA ICM results have stronger discriminative power than DNA FCM for grade in TaT1 UCs.</p>","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 3","pages":"147-53"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/410239","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22409638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstracts of the International Conference on Applied Genomics, 9th ESACP/16th ISDQP Meeting. Amsterdam, The Netherlands, 1-4 October 2003.","authors":"","doi":"10.1155/2003/207868","DOIUrl":"https://doi.org/10.1155/2003/207868","url":null,"abstract":"On behalf of the European Society for Analytical Cellular Pathology and the International Society for Diagnostic Quantitative Pathology, we warmly welcome you to the International Conference on Applied Genomics in Amsterdam. The genomics revolution has had an enormous impact on biomedical sciences in general and several disease areas in particular. Genomics research has yielded unprecedented insights into basic mechanisms of diseases such as cancer, degenerative diseases and congenital disorders. The current challenge to the medicalscientific community is to carry these new insights further and to translate them into new diagnostic, prognostic and therapeutic applications in patient management. Indeed, in diagnostic surgical pathology, which has traditionally depended on the use of phenotypical classification systems, genomics-based classifications are now emerging. In cancer patients, features like histological type and tumor differentiation grade, sometimes combined with additional markers like proliferation, DNA ploidy or immunohistochemical markers, have routinely been employed for classification and as determinants of prognosis and therapy. Now we are witnessing the emergence of genetic tumor profiling as the basis for tumor classification and clinical decision making in oncology leading to revolutionary shifts in diagnostic and therapeutic practice. The results of translational research on the application of genomics will urge us to substantially rewrite parts of pathology, oncology and other medical disciplines in the years to come. Genetic tumor profiling has already led to, and will continue to lead to the identification of new molecular targets for innovative anticancer agents. Pharmaceutical and biotechnology companies have introduced high-throughput micro-array based screening to identify new anticancer drugs acting at newly identified molecular targets. Indeed, several new agents designed to act at specific genes or gene products that are frequently altered in cancer, have entered clinical evaluation in cancer patients. In the near future, several of these agents are likely to become available for use in daily practice. For physician involved in the treatment of cancer patients, it will therefore become increasingly important to obtain information on the tumor’s genetic and proteomic profile as pivotal input for the patient’s treatment plan. Also the evolution of the European Society of Analytical Cellular Pathology and the International Society for Diagnostic Quantitative Pathology into one new society fits in this scheme. The new International Society for Cellular Oncology that will be founded during this meeting, exactly focuses on the challenges to medical sciences described above, and will provide a powerful forum for scientist in this area. The International Conference on Applied Genomics brings together a broad variety of biomedical and clinical disciplines involved in, and affected by the genomics revolution. The latest genom","PeriodicalId":76996,"journal":{"name":"Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology","volume":"25 5-6","pages":"215-317"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2003/207868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}