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Reproducibility of a fractionation procedure for fish muscle proteomics 鱼类肌肉蛋白质组学分离程序的可重复性
American biotechnology laboratory Pub Date : 2009-01-01 DOI: 10.6084/M9.FIGSHARE.1301104.V1
T. Silva, Odete D. Cordeiro, F. Jessen, Jorge Dias, P. Rodrigues
{"title":"Reproducibility of a fractionation procedure for fish muscle proteomics","authors":"T. Silva, Odete D. Cordeiro, F. Jessen, Jorge Dias, P. Rodrigues","doi":"10.6084/M9.FIGSHARE.1301104.V1","DOIUrl":"https://doi.org/10.6084/M9.FIGSHARE.1301104.V1","url":null,"abstract":"One of the challenges of comparative proteomics is the ability to separate and quantify all the components of highly complex mixtures of proteins, despite the fact that the concentration of their components can span several orders of magnitude. Subcellular fractionation procedures for muscle tissue have been used for some time in both gel and MS-based proteomics to address this situation, simplifying analysis by reducing the number of proteins in a given extract and improving the dynamic range by allowing larger loads per protein. Additionally, interpreting the observed changes in protein abundance may be eased by isolating a subpopulation of the whole tissue proteome. Specifi c examples of the use of these techniques for proteomic analysis include organelle and nucleus isolation, enrichment of extracts for secretory or membranar proteins, and albumin/immunoglobulin depletion of serum samples. On the other hand, since its use implies a greater number of sample processing steps than doing the whole extract, it is expected that some noise may be introduced by a fractionation procedure. Therefore, it seems important for comparative proteomic experiments to simultaneously ensure that no interbatch bias is introduced and that intrabatch noise is minimized, so that the benefits of performing this step outweigh its disadvantages.","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"28 1","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71206197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Total internal reflection fluorescence microscopy 全内反射荧光显微镜
American biotechnology laboratory Pub Date : 1999-01-01 DOI: 10.1007/springerreference_67846
Y. Kawano, R. Enders
{"title":"Total internal reflection fluorescence microscopy","authors":"Y. Kawano, R. Enders","doi":"10.1007/springerreference_67846","DOIUrl":"https://doi.org/10.1007/springerreference_67846","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"17 1","pages":"28-30"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52983533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experimental strategies enabled by C-terminal protein sequence analysis. c端蛋白序列分析实现的实验策略。
American biotechnology laboratory Pub Date : 1996-06-01
C G Miller, J M Bailey, K Swiderek, J W Kenny, P C Andrews, C Mitchell, J Tropea
{"title":"Experimental strategies enabled by C-terminal protein sequence analysis.","authors":"C G Miller, J M Bailey, K Swiderek, J W Kenny, P C Andrews, C Mitchell, J Tropea","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"14 7","pages":"8, 12"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19677073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Automated peptide mapping using a multidimensional chromatography workstation. 使用多维色谱工作站的自动肽图谱。
American biotechnology laboratory Pub Date : 1996-04-01
N Gordon, T Nadler, C Blackburn, H Wang, J Speak
{"title":"Automated peptide mapping using a multidimensional chromatography workstation.","authors":"N Gordon, T Nadler, C Blackburn, H Wang, J Speak","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"14 5","pages":"18, 20"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19898267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Affinity chromatography: production and regulatory considerations. 亲和色谱法:生产和监管方面的考虑。
American biotechnology laboratory Pub Date : 1996-04-01
S J Burton
{"title":"Affinity chromatography: production and regulatory considerations.","authors":"S J Burton","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"14 5","pages":"64-6"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19898269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytotoxicity quantification with a cell counter and analyzer system. 用细胞计数器和分析系统进行细胞毒性定量。
American biotechnology laboratory Pub Date : 1996-04-01
B Glauner
{"title":"Cytotoxicity quantification with a cell counter and analyzer system.","authors":"B Glauner","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"14 5","pages":"24, 26"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19898268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vitamin analysis using capillary zone electrophoresis. 毛细管区带电泳分析维生素。
American biotechnology laboratory Pub Date : 1996-03-01
R Neubert, J Schiewe
{"title":"Vitamin analysis using capillary zone electrophoresis.","authors":"R Neubert, J Schiewe","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"14 4","pages":"12, 14"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19834375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HPCE separation of protected amino acids and peptides. 保护氨基酸和肽的高效液相色谱分离。
American biotechnology laboratory Pub Date : 1996-01-01
B L Zhang, R H Yang, S Jin
{"title":"HPCE separation of protected amino acids and peptides.","authors":"B L Zhang, R H Yang, S Jin","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"14 1","pages":"32"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19559816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An enzyme array for sialylated N-glycans. 用于唾液化n -聚糖的酶阵列。
American biotechnology laboratory Pub Date : 1995-12-01
J Bruce, A Venton, N Shipston, S Prime
{"title":"An enzyme array for sialylated N-glycans.","authors":"J Bruce, A Venton, N Shipston, S Prime","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"13 13","pages":"26-8"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19535548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expanding the scope of applications for C-terminal sequencing. 扩大了c端测序的应用范围。
American biotechnology laboratory Pub Date : 1995-10-01
C G Miller, J M Bailey
{"title":"Expanding the scope of applications for C-terminal sequencing.","authors":"C G Miller, J M Bailey","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76989,"journal":{"name":"American biotechnology laboratory","volume":"13 11","pages":"36-7"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18582171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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