{"title":"Analyses of the tidemark on human femoral heads. I. Histochemical, ultrastructural and microanalytic characterization of the normal structure of the intercartilaginous junction.","authors":"R Oettmeier, K Abendroth, S Oettmeier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The tidemark as a boundary between non-calcified and calcified articular cartilage has a complicated structure and fulfils various physiological functions. 25 human femoral heads from healthy joints were examined. In non-decalcified preparations three structural components of the tidemark were demarcated: (1) the PAS-positive, proteoglycan-containing tidemark line adjacent to the basal cartilage, (2) the sublinear light-coloured zone and (3) the demarcation line to the calcified cartilage. Using scanning electron microscopy, the tidemark was characterized as an electron-dense impression between hyaline and calcified cartilage. The presence of specific architecture and orientation of collagenous fibres could not be shown. Concentrations of calcium, phosphorus and sulphur could not be detected in the tidemark by means of an X-ray microanalyzer. A morphological model of the tidemark region was established, which represents the intercartilaginous junction as a double layer of substantially and functionally different surfaces.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 3-4","pages":"155-68"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphology of supravital brain slices pre-incubated in a physiological solution prior to fixation.","authors":"F Hajós, J Garthwaite, G Garthwaite, A Csillag","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Slices of 400 microns thickness were prepared from various regions of the adult rat brain using a vibratome, and incubated in oxygenated Krebs-Henseleit medium for 3 h prior to processing for light and electron microscopy. Previous findings in cerebellar slices suggest that the structural recovery requires at least a 1 h period of incubation. In the present study, vibratome-cut slices of other brain areas such as the hippocampus, parietal cortex, striatum and median eminence were shown to display after this period a gross and fine structure comparable to in situ fixed tissues, and to retain it for at least 3 h of incubation. These areas are thought to be promising for further in vitro functional morphological experimentation.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 3-4","pages":"181-99"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relative growth of the human temporal bone in the prenatal period.","authors":"C A Mandarim-de-Lacerda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Thirty right temporal bones of Brazilian fetuses (ranging from 14th to 36th weeks) were studied by the allometric method in order to define the pattern of the whole temporal bone weight growth and the weight growth of the temporal bone portions: squamous, petrous and tympanic. These data were compared to fetal weight. The increase of the data of the temporal bone shows a positive allometry except for the tympanic portion (isometry). The squamous portion shows the highest allometric coefficient. These data are important to the knowledge of the human skull growth.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 1-2","pages":"65-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13662064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimentally induced acute changes in veins studied by scanning microscopy.","authors":"U Fuchs, H Jellinek, A Kádár","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Endothelial damage caused by hypoxia and the local administration of Varicocid, or by using vascular clamps on the jugular vein of rabbits, was studied under a scanning electron microscope. Adhering platelets and fibrin were found only in the acutely damaged veins with endothelial defects. Swelling and vacuolization of endothelial cells did not induce intravascular coagulation. Adhering thrombocyte aggregates and fibrin strands can be found on the venous wall after acute changes under a scanning microscope only if part or all of the endothelial lining has been lost. Intravascular coagulation is not induced by swelling or vacuolization. The jugular vein of rabbits was studied after hypoxia, the administration of Varicocid and the use of vascular clamps.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 1-2","pages":"93-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immune complex nephropathies in patients with malignant tumours.","authors":"L Kun, J Nagy, M Schmelczer, C Trinn, T Burger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors have studied the incidence of changes related to immune complex glomerulonephritis in postmortem kidney specimens from 23 patients with malignant tumours (18 solid tumours and 5 leukaemias), using light microscopy and immunofluorescence. As revealed by light microscopy, 4 kidneys had diffuse, 2 focal mesangial proliferative glomerulonephritis and 1 IgA glomerulonephritis. Granular deposition of immunoglobulin in the mesangium was observed in 5 kidneys, on 3 occasions together with C3. In only 2 patients were, in addition to glomerular damage, clinical symptoms observed. No CEA and alpha FP were found in the diseased kidneys. The results suggest that glomerular damage with no or only occasional clinical symptoms is not rare in patients with malignant tumours.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 1-2","pages":"101-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13678502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Vojtísek, D Hulínská, D Bittnerová, M Cikrt, V Hulínský
{"title":"Cadmium influence on the rat liver.","authors":"M Vojtísek, D Hulínská, D Bittnerová, M Cikrt, V Hulínský","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Influence of cadmium on the intestinal and hepatic tissue of rat was studied in acute injection and oral as well as subchronic and chronic oral experiments with the purpose of better understanding of the penetration mechanisms of cadmium into these tissues. Acute single oral dose of CdCl2 32.5 mg.kg-1 inhibited in vitro the respiration of intestinal scrapings (44%) and in liver slices (25.7%). In combined oral doses of 32.5 mg.kg-1 + 162.5 mg.kg-1 inhibition in the intestine increased with exposure time, while in the liver it decreased. Amount of presumable metallothionein in the liver was approximately ten times higher than that in the intestine. Respiration was measured by a Clark electrode. Binding of cadmium to metallothionein was determined by column chromatography and spectrophotometry. Physiological findings are in agreement with morphological ones. In cases when, following an acute single oral dose, a 50% change was detected in the absorptive jejunal zone, a 10% change was observed in the liver parenchyma. In the case of combined oral dose of 60-70% of absorptive villi zone is damaged by cadmium, which penetrates by passive diffusion into lamina propria and by blood to the liver, where it acts toxically in 30%. Active transport of cadmium after chronic application is preserved. Correlation of physiological and morphological findings was evident.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 3-4","pages":"201-17"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13631131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dendritic changes in the hippocampus of aged rats.","authors":"I Lolova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Age-related changes in dendritic branches in stratum lacunosum-moleculare of the CA1 hippocampal field were examined using quantitative ultrastructural methods. A decrease by 38% was demonstrated in the number of dendritic branch profiles per tissue square area in the group of aged (24-month-old) rats as compared to that in the group of young adult (3-month-old) rats. The total volume fraction and total surface of dendrites per volume neuropil were decreased by 23% and 35%, respectively in aged rats. The percentage area of neuropil occupied by dendritic branch profiles was smaller in aged rats (by 22%) than that in young adult rats. The width of the layers including the apical dendrites of the CA1 hippocampal pyramidal cells was unchanged in the group of aged rats. The degree of the changes in different parameters examined suggests that the atrophy and loss of dendrites in aging hippocampus affect preferentially terminal branches.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 1-2","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13662062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Alteration of the submandibular glands of female mice during pregnancy and lactation. A histological, histometric and histochemical study.","authors":"J A Rossi, L C Bruschi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The submandibular glands of mice show \"masculinization\" during pregnancy and lactation. In this work involving morphological, morphometric and histochemical analyses we observed an increase in the volume fraction and area of the granular ducts and the apical granulation of their cells. The maximum response to the masculinization occurred on the 15th day of pregnancy. From this day on a decrease in the volume fraction of the granular ducts starts, even though the gland keeps the \"masculine pattern\" throughout the end of the lactation phase. Our observations support the intermodulation between striated and granular ducts. In our opinion, the \"masculinization\" process of the submandibular gland is related to the progesterone increase which takes place during pregnancy; its maintenance during lactation is also connected to prolactin.</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 3-4","pages":"135-45"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13630415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Haemophagocytic sinus histiocytosis with massive lymphadenopathy: early lesions, progression and involution.","authors":"G Kelényi, L Olasz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In a 26-year-old male suffering from haemophagocytic sinus histiocytosis with massive lymphadenopathy (SHML) five biopsies were performed during a period of 60 months. It seemed that the first change in SHML is the appearance of foamy histiocytes in the subcapsular sinuses at a time when accumulation of and haemophagocytosis by the histiocytes in the intermediary and medullary sinuses are not conspicuous. Progression of the lesions is indicated by the accumulation of haemophagocytic histiocytes in all sinuses and by the development of subcapsular xanthofibrous tissue areas, first focal, later confluent. Regression of the lymphadenomegaly may be due to gradual obliteration of the lymph node parenchyma by the subcapsular xanthofibrous tissue and by scarring. Even in the early phases of the disease the paracortical areas were underdeveloped. The immunophenotype of the foamy histiocytes populating the sinuses and the xanthofibrous areas is S-100+, Leu-M3+, VIM-13+ (CD 14), MAC 387+ N-PNA+, OKIa+, Leu-M1- (CD 15) and OKM1- (CD 11).</p>","PeriodicalId":76971,"journal":{"name":"Acta morphologica Hungarica","volume":"37 3-4","pages":"265-75"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13631135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}