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Acta microbiologica Hungarica最新文献

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Latex agglutination and adenoviruses. I. Detection of polyclonal antibodies by latex agglutination test. 乳胶凝集和腺病毒。1 .乳胶凝集试验检测多克隆抗体。
Acta microbiologica Hungarica Pub Date : 1992-01-01
A Lengyel, I Nász, E Adám
{"title":"Latex agglutination and adenoviruses. I. Detection of polyclonal antibodies by latex agglutination test.","authors":"A Lengyel,&nbsp;I Nász,&nbsp;E Adám","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A latex agglutination (LA) test was developed for the detection and measurement of polyclonal anti-adenovirus antibodies. Latex particles, coated with purified hexon, penton or fibre antigens displayed specific agglutination with 21 rabbit immune sera directed against the different antigens and the complete virus of 10 human and 2 animal adenovirus types. The sensitivity of LA was compared to that of passive haemagglutination, complement fixation (CF) and immunodiffusion reactions. In experiments with immune sera as well as with human serum samples the sensitivity of LA test proved to be in the same range as that of CF test. Because of its easy and rapid performance (slide agglutination within 4 min) LA method may substitute for CF reaction in serological adenovirus diagnosis.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 3-4","pages":"303-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12517851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Colonization of infant mice with flagellar variants of Campylobacter jejuni. 空肠弯曲杆菌鞭毛变异幼鼠的定植。
Acta microbiologica Hungarica Pub Date : 1992-01-01
K S Diker, G Hascelik, S Diker
{"title":"Colonization of infant mice with flagellar variants of Campylobacter jejuni.","authors":"K S Diker,&nbsp;G Hascelik,&nbsp;S Diker","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The role of flagella in the colonization of the intestine by Campylobacter jejuni was investigated by challenging infant mice with two flagellated strains and their nonflagellated variants. The intestinal tracts of infant mice were regularly colonized with motile strains, but not by nonmotile variants. Colonization of mice with motile C. jejuni occurred with as few as 1000 bacteria per mouse.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 2","pages":"133-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12482274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiple enlargements in the right inverted terminal repeat of the DNA of canine adenovirus type 2. 犬腺病毒2型DNA右端反向重复序列多次扩增。
Acta microbiologica Hungarica Pub Date : 1992-01-01
G Fejér, G Berencsi, Z Ruzsics, S Belák, T Linné, I Nász
{"title":"Multiple enlargements in the right inverted terminal repeat of the DNA of canine adenovirus type 2.","authors":"G Fejér,&nbsp;G Berencsi,&nbsp;Z Ruzsics,&nbsp;S Belák,&nbsp;T Linné,&nbsp;I Nász","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Manhattan strain of canine adenovirus type 2 (CAV 2) was examined. Restriction endonuclease analysis and blot hybridization experiments revealed the heterogeneity of the viral DNA. At least 9 unequally expanded species of the viral genome have been recognized. This diversity is caused by different enlargements in the right inverted terminal repeat (ITR) of the virus. The differences between the individual enlargements were shown to be the different multiples of 150 base pairs. Relatedness of CAV 2 DNA to the DNA of bovine adenovirus type 2 (BAV 2) and human adenovirus type 2 (HAV 2) has also been observed during DNA hybridization experiments.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 2","pages":"159-68"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12482277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishment of an MT4 cell line persistently producing infective HIV-1 particles. 持续产生HIV-1病毒颗粒的MT4细胞系的建立。
Acta microbiologica Hungarica Pub Date : 1992-01-01
A Gyuris, G Vajda, I Földes
{"title":"Establishment of an MT4 cell line persistently producing infective HIV-1 particles.","authors":"A Gyuris,&nbsp;G Vajda,&nbsp;I Földes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human T cell Lymphotropic Virus-I (HTLV-I) carrying human T cell line MT4 is highly sensitive to Human Immunodeficiency Virus-1 (HIV-1). After HIV-1 infection cell clusters characteristic of intact MT4 rapidly disintegrate, syncytia appear and the cells die. Surviving MT4 cells were subcultured following HIV-1 infection of high multiplicity. We succeeded to establish an MT4 cell line continuously producing infective HIV (MT4/HIV-1). The original and the HIV-1 infected MT4 cells were morphologically similar. The MT4/HIV-1 cells proved to be nearly 100% positive in indirect immunofluorescence assay using the serum of an HIV-1 antibody positive individual. OKT4 surface antigen could not be demonstrated on MT4/HIV-1 cells. On electron microscopic pictures typical and atypical virus particles could be seen near the surface of the cell membrane. The persistently produced virus particles were infective for H9 and MT4 cells. The antigenic structure of the virus produced by MT4 cells was similar to that produced by H9 cells.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 3-4","pages":"271-9"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12537279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Annual Meeting of the Hungarian Society for Microbiology. Szekesfehervar, July 7-9, 1992. Abstracts. 匈牙利微生物学会年会。1992年7月7日至9日，谢克斯费瓦尔。摘要。
Acta microbiologica Hungarica Pub Date : 1992-01-01
{"title":"Annual Meeting of the Hungarian Society for Microbiology. Szekesfehervar, July 7-9, 1992. Abstracts.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 3-4","pages":"327-87"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12537280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Model examination of selective media for isolation of Listeria strains. 李斯特菌选择培养基的模型检验。
Acta microbiologica Hungarica Pub Date : 1991-01-01
H Domján Kovács, B Ralovich
{"title":"Model examination of selective media for isolation of Listeria strains.","authors":"H Domján Kovács,&nbsp;B Ralovich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>During the Tenth International Symposium on Listeriosis (Pécs, Hungary, 1988) the Working Party on Culture Media of IUMS-ICFMH suggested comparative examination of nine enrichment broths and nine solid selective media. On the basis of this proposal the following media were studied: LiCl-phenylethanol-moxalactam agar (LPM), polymyxin-acriflavine-LiCl-ceftazidime-aesculin-mannitol agar (PALCAM) No. 1 (home made) and No. 2 (Merck), acriflavine-ceftazidime agar (AC), Oxford agar, tripaflavine-nalidixic acid serum agar (TNSA) and Forray's agar. The study was performed as described in \"Testing methods for use in quality assurance of culture media\". Oxford agar proved to be the best medium. LPM, AC and Forray's agars were somewhat more inhibitory than Oxford medium. In productivity TNSA and PALCAM media were weakest but the latter one was more selective. When 43 sausage samples were enriched in UVM broths and subcultured on the above mentioned media the number of positive samples was the same on Oxford, LPM, AC and TNSA agars but it was lower on PALCAM agar No. 1. When 103 milk samples were subcultured on TNSA and PALCAM agar No. 2, the number of positive samples was the same.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 2","pages":"141-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12967210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of chromogenic and fluorogenic substances for differentiation of coliforms and Escherichia coli in soft cheese. 软奶酪中大肠菌群和大肠杆菌的显色和荧光物质鉴别比较。
Acta microbiologica Hungarica Pub Date : 1991-01-01
G Hahn, E Wittrock
{"title":"Comparison of chromogenic and fluorogenic substances for differentiation of coliforms and Escherichia coli in soft cheese.","authors":"G Hahn,&nbsp;E Wittrock","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In food hygiene the differentiation of Escherichia coli and coliforms as index resp. indicator organisms is very important as a basis for the assessment of good manufacturing practice (gmp). Using a fluorogenic (MUG) and chromogenic (X-gal) substrate the laborious methods have become more simple and reliable. A new German DIN standard method (10.183, part 3) was used as a base to examine 200 soft cheese samples for coliforms and Escherichia coli comparing different media, parameters and incubation times. It could be shown that an incubation of 48 h is absolutely necessary for E. coli and coliforms in both media, that X-gal is a quicker and more sensitive parameter for total coliforms than gas production and that the combination of fluorescence and indole is slightly superior to fluorescence and gas for the identification of E. coli. To sum up, the new fluorescence principle, integrated in new standard methods will be an excellent tool to simplify the differentiation of E. coli and coliforms in food hygiene. Additionally, X-gal as a chromogenic substrate for all coliforms, including E. coli, may be integrated in further standard methods.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 3-4","pages":"265-71"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid identification of Escherichia coli from urine by using Fluorocult media. 用荧光培养基快速鉴定尿液中的大肠杆菌。
Acta microbiologica Hungarica Pub Date : 1991-01-01
T Mori, H Takahashi, E Maehata, H Naka
{"title":"Rapid identification of Escherichia coli from urine by using Fluorocult media.","authors":"T Mori,&nbsp;H Takahashi,&nbsp;E Maehata,&nbsp;H Naka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>For rapid identification of Escherichia coli, we evaluated Fluorocult MacConkey Agar, Fluorocult Laurylsulfate Broth and Bactident E. coli, which are incorporating fluorogenic substrate, MUG (4-methylumbeliferyl-beta-D-Glucuronide) that specifically reacts with E. coli. To assess the specificity and sensitivity of Fluorocult MacConkey Agar and Laurylsulfate Broth, beta-D-glucuronidase; beta-GUR activities of 264 strains from urine including 72 of E. coli were investigated. For both media, sensitivity was 92% and specificity was 100%. When there was 10(8) c.f.u./ml of E. coli in urine specimen, incubation times required for positive fluorescence by Fluorocult MacConkey Agar, Laurylsulfate Broth, and Bactident E. coli were 8 h, 4 h and 15 min, respectively. Influence of drugs in urine to fluorescence reaction was not observed.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 3-4","pages":"273-81"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
beta-D-glucuronidase (BDG) activity of gram-negative bacteria. 革兰氏阴性菌β - d -葡萄糖醛酸酶(BDG)活性。
Acta microbiologica Hungarica Pub Date : 1991-01-01
B Ralovich, G A Ibrahim, A Fábián, M Herpay
{"title":"beta-D-glucuronidase (BDG) activity of gram-negative bacteria.","authors":"B Ralovich,&nbsp;G A Ibrahim,&nbsp;A Fábián,&nbsp;M Herpay","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>BDG is an inducible enzyme that is encoded by the uidA gene in Escherichia coli. Genetic sequences of this gene are present in most if not all E. coli strains regardless of the BDG phenotype. Expression of BDG activity can be influenced by lactose-induced catabolite repression or genetic mutations. Salmonella, Shigella and Yersinia strains frequently exhibit positive BDG reaction. BDG activity of strains belonging to genus Edwardsiella, Serratia, Yersinia, Vibrio, Erwinia, Alcaligenes, Acinetobacter, Moraxella, Plesiomonas, Achromobacter, Flavobacterium, Chromobacterium and Pasteurella awaits examination.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 3-4","pages":"283-91"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resistance of Escherichia coli to some antibiotics and biocides in the intestinal biofilm of mice. 小鼠肠生物膜中大肠杆菌对某些抗生素和杀菌剂的耐药性。
Acta microbiologica Hungarica Pub Date : 1991-01-01
I Kétyi
{"title":"Resistance of Escherichia coli to some antibiotics and biocides in the intestinal biofilm of mice.","authors":"I Kétyi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An artificial monoflora of Escherichia coli in mice, as well as their autochtonous E. coli, exhibited enhanced resistance to streptomycin, chloramphenicol, sodium hypochlorite and silver nitrate. The level of resistance of the monoflora, which was 10-32 times higher than the in vitro determined Minimal Bactericidal Dose (MBCD), reached its maximum on the 7th-9th day after implantation. This latency is a requirement for the stabilization of the monoflora. Formaldehyde and carbenicillin were equally effective in the planktonic and in the biofilm mode of growth. In the case of carbenicillin the pieces of mouse colon contained about 60% of the dose used for exposure, in contrast to the 3% rate of streptomycin, showing the excellent penetrating ability of carbenicillin into the intestinal biofilm.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 1","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12972866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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