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Acta microbiologica Hungarica最新文献

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Heat resistance of Listeria monocytogenes in naturally infected and inoculated cow's milk. 自然感染和接种的牛奶中单核细胞增生李斯特菌的耐热性。
Acta microbiologica Hungarica Pub Date : 1991-01-01
I Kovincić, M Mrdjen, V Komnenov-Pupovac, I F Vujicić, M Vulić, M Svabić-Vlahović, J T Tierney
{"title":"Heat resistance of Listeria monocytogenes in naturally infected and inoculated cow's milk.","authors":"I Kovincić,&nbsp;M Mrdjen,&nbsp;V Komnenov-Pupovac,&nbsp;I F Vujicić,&nbsp;M Vulić,&nbsp;M Svabić-Vlahović,&nbsp;J T Tierney","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A two phase slug flow tubular heat exchanger was used for the thermal inactivation of Listeria monocytogenes in natural infected milk from seven cows. L. monocytogenes serotype 4b inoculated UHT sterilized milk was monitored in a parallel study. The two milks were heated at 71.1 degrees C for holding times of 2, 4, 10, 15, 20 and 30 s. Milk was assayed for survivors immediately after heat treatment and weekly thereafter for 4 weeks during storage at 4 degrees C. No survivors were detected in the naturally infected milk at any of the holding times. Survivors were found at 2 and 4 s in the inoculated UHT milk with initial titres of 8 x 10(2) to 7.1 x 10(3) c.f.u./ml, only after storage at 4 degrees C for 28 days. No survivors were detected for 10 through 30 s holding times.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 1","pages":"3-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12972865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathogenic Escherichia coli O157:H7 and their detection. 致病性大肠杆菌O157:H7及其检测。
Acta microbiologica Hungarica Pub Date : 1991-01-01
M Pawelzik
{"title":"Pathogenic Escherichia coli O157:H7 and their detection.","authors":"M Pawelzik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enterohaemorrhagic Escherichia coli O157:H7 is of major concern to the food industry due to high pathogenicity of this foodborne organism. For the detection of these bacteria a special agar medium with a fluorogenic substrate has been developed. The medium uses the characteristics of this E. coli serotype not to ferment sorbitol and not to produce beta-glucuronidase. In contrast, approximately 96% of all other strains of E. coli are sorbitol-positive and nearly all of them are beta-glucuronidase-positive. For discrimination between Proteus and E. coli O157:H7 which are both sorbitol- and beta-glucuronidase-negative, sodium thiosulphate and ferrio ammonium citrate were added. This leads to a brownish colour of the Proteus colonies due to their production of hydrogen sulphide. Growth of the gram-positive flora was inhibited by the addition of sodium deoxycholate.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 3-4","pages":"315-20"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relative surface hydrophobicity, antigen K1 and haemagglutinating activity are associated in Escherichia coli. 相对表面疏水性，抗原K1和血凝活性与大肠杆菌有关。
Acta microbiologica Hungarica Pub Date : 1991-01-01
M Herpay, E Czirók, E Szöllösy, J Fekete, I Gadó, H Milch
{"title":"Relative surface hydrophobicity, antigen K1 and haemagglutinating activity are associated in Escherichia coli.","authors":"M Herpay,&nbsp;E Czirók,&nbsp;E Szöllösy,&nbsp;J Fekete,&nbsp;I Gadó,&nbsp;H Milch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hydrophobic property of 136 Escherichia coli strains was examined by salt aggregation test (SAT). Out of the tested strains 61 were SAT positive. The correlations among the surface properties characterized by SAT and other phenotypical properties, e.g. mannose resistant haemagglutinating activity (MRHA), mannose sensitive haemagglutinating activity (MSHA), presence of antigen K1 and adsorption to Al(OH)3 gel were examined. The results showed that (i) Possession of antigen K1 provides the bacterial cell a hydrophilic character and covers its relative surface hydrophobicity; (ii) Correlation exists between the relative hydrophobicity of the bacteria determined by SAT and their haemagglutinating activity. SAT values are also influenced by non haemagglutinating fimbriae and also by other non fimbrial structures; (iii) The hydrophilic surface characters are mainly expressed by the results of adsorption to Al(OH)3 gel and the hydrophobic characters rather by the SAT values.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 1","pages":"17-28"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12972862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Connective tissue content of fibrolamellar carcinoma and other human liver tumors. 纤维板层癌及其他人类肝脏肿瘤的结缔组织含量。
Acta microbiologica Hungarica Pub Date : 1991-01-01
I Sárosi, A Kiss, Z Schaff, K Lapis
{"title":"Connective tissue content of fibrolamellar carcinoma and other human liver tumors.","authors":"I Sárosi,&nbsp;A Kiss,&nbsp;Z Schaff,&nbsp;K Lapis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The connective tissue content of four different human liver tumors (Fibrolamellar carcinoma, FLC; hepatocellular carcinoma, HCC; focal nodular hyerplasia, FNH and hepatocellular adenoma, HCA) was investigated by computer aided morphometry. A significantly higher connective tissue content was found in FNH and FLC as compared to HCA and HCC. The distribution of the connective tissue was homogeneous in the cases of FNH, HCA and HCC, while a highly unhomogeneous distribution was observed in FLC cases.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 4","pages":"321-31"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunoelectron microscopic localization of the p21 protein in HT1080 human fibrosarcoma cell lines with altered N-ras gene expression. N-ras基因表达改变的HT1080人纤维肉瘤细胞系中p21蛋白的免疫电镜定位
Acta microbiologica Hungarica Pub Date : 1991-01-01
J Tímár, H Paterson
{"title":"Immunoelectron microscopic localization of the p21 protein in HT1080 human fibrosarcoma cell lines with altered N-ras gene expression.","authors":"J Tímár,&nbsp;H Paterson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cellular localization of the p21 protein was studied by immunoelectron microscopy in human fibrosarcoma cells with different N-ras gene expression. The tumorigenic HT1080 cells--expressing equally the normal and mutant N-ras genes--contained the p21 in the plasma membrane, in pinocytotic areas as well as in the intracellular vesicles. The non-tumorigenic revertants--expressing more normal N-ras gene than the mutant one--contained p21 in the plasma membrane, especially at the cell junctions. These results suggest, that the mutant p21 may locate to the membrane areas connected with pino- or endocytotic functions.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 4","pages":"259-65"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13005418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure and macromolecular composition of the myotendineal junction. Histochemical, immunohistochemical and electron microscopic study of the rat calf muscles. 肌腱连接的结构和大分子组成。大鼠小腿肌肉组织化学、免疫组织化学及电镜研究。
Acta microbiologica Hungarica Pub Date : 1991-01-01
L Józsa, M Kvist, P Kannus, T Vieno, M Järvinen, M Lehto
{"title":"Structure and macromolecular composition of the myotendineal junction. Histochemical, immunohistochemical and electron microscopic study of the rat calf muscles.","authors":"L Józsa,&nbsp;M Kvist,&nbsp;P Kannus,&nbsp;T Vieno,&nbsp;M Järvinen,&nbsp;M Lehto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The macromolecular composition and ultrastructure of the myotendineal junction (MTJ) of slow-twitch (type 1) and fast-twitch (type 2) muscle fibers were studied in the gastrocnemius-soleus-Achilles unit of the rat. Both proteoglycans and glycosaminoglycans, type III collagen, fibronectin and laminin could be detected at the MTJ. Due to membrane folding, finger-like processes were seen at the myotendineal junction. The processes of the type 1 fibers are greater in size, however, due to subdivisions, the processes of type 2 muscle fibers had a greater surface than type 1 fibers. The macromolecular composition is similar in both type 1 and type 2 muscle fibers.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 4","pages":"287-97"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13006048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of an infectious oncogenic adenovirus strain from the stimulated lymphocytes of a patient with bladder tumour. 从膀胱肿瘤患者受刺激淋巴细胞中分离出一株感染性致癌腺病毒。
Acta microbiologica Hungarica Pub Date : 1991-01-01
G Kulcsár, S Csata, I Nász
{"title":"Isolation of an infectious oncogenic adenovirus strain from the stimulated lymphocytes of a patient with bladder tumour.","authors":"G Kulcsár,&nbsp;S Csata,&nbsp;I Nász","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In attempts to grow viruses from tumour cells and circulating lymphocytes, we isolated an oncogenic infectious adenovirus type 18 strain from the phytohaemagglutinin-stimulated peripheral T-lymphocytes of a patient with bladder tumour. The virus genome is assumed to play certain role in the development of the tumour in interaction with other DNA viruses and the damaged immune system.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 2","pages":"91-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12967214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binding of lectins (Con-A, lens, helix, PHA) to binding sites induced in Tetrahymena by insulin and lectins. 胰岛素和凝集素诱导四膜虫结合位点的凝集素(Con-A，透镜体，螺旋体，PHA)结合。
Acta microbiologica Hungarica Pub Date : 1991-01-01
G Csaba, P Kovács
{"title":"Binding of lectins (Con-A, lens, helix, PHA) to binding sites induced in Tetrahymena by insulin and lectins.","authors":"G Csaba,&nbsp;P Kovács","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Concanavalin-A (Con-A) gave rise to a moderate aggregation in untreated (control) Tetrahymena pyriformis GL populations. The cells pretreated (imprinted) with insulin or lectins underwent aggregation earlier and to a greater degree than the untreated control cells. Lens lectin, although of the same sugar specificity as Con-A, was considerably less active than the latter. Helix lectin and phytohaemagglutinin (PHA), although of similar sugar specificity, accounted for a dissimilar extent of cell aggregation, in that Helix lectin was comparable in activity to Con-A. Pretreatment (imprinting) with insulin enhanced the aggregation inducing effect of those lectins which were capable of overlapping on the insulin receptor owing to similar specificity.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 1","pages":"29-32"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12972863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A mathematical method for the identification of the ATGCAT recognition sequence of a Streptococcus mutans restriction endonuclease. 变异链球菌限制性内切酶ATGCAT识别序列的数学鉴定方法。
Acta microbiologica Hungarica Pub Date : 1991-01-01
P Geck, A Molnár, I Nász
{"title":"A mathematical method for the identification of the ATGCAT recognition sequence of a Streptococcus mutans restriction endonuclease.","authors":"P Geck,&nbsp;A Molnár,&nbsp;I Nász","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Taking advantage of a mathematical equation applied so far in different fields the calculation of ATGCAT recognition sequence of restriction endonuclease from Streptococcus mutans serotype C (SmuC I) is reported together with confirming computer and physical mapping data.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 1","pages":"43-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12972867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of neonatal benzpyrene pretreatment (imprinting) on the hepatic 3H-benzpyrene intake and output in adult rat liver. Investigation into the hepatic localization of benzpyrene in adult rats. 新生儿苯并芘预处理(印迹)对成年大鼠肝脏3h -苯并芘摄入和输出的影响。苯并芘在成年大鼠肝脏定位的研究。
Acta microbiologica Hungarica Pub Date : 1991-01-01
A K Fülöp, G Csaba
{"title":"Impact of neonatal benzpyrene pretreatment (imprinting) on the hepatic 3H-benzpyrene intake and output in adult rat liver. Investigation into the hepatic localization of benzpyrene in adult rats.","authors":"A K Fülöp,&nbsp;G Csaba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One hour after treatment with 3H-benzpyrene, grains appeared for the most part above the hepatocellular surface, heterochromatin and dense vacuoles, whereas 24 h later they were associated mainly with the dense vacuoles and smooth-surfaced endoplasmic reticulum and only, a few were still present above the heterochromatin. Neonatal pretreatment (imprinting) with benzpyrene accounted for an earlier appearance of benzpyrene in the hepatocytes relative to the non-pretreated control, but while labelled benzpyrene had practically disappeared from the liver of imprinted rats within 24 h, it still persisted in the liver of control rats at a level approximating the 4 h value.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 4","pages":"279-85"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13006046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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