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Acta microbiologica Hungarica最新文献

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The course of LCMV infection in euthymic and athymic mice pretreated with immunomodulatory agents. 免疫调节剂预处理健康型和胸腺型小鼠的LCMV感染过程。
Acta microbiologica Hungarica Pub Date : 1992-01-01
P Anderlik, I Szeri, Z Bános, Z Barna
{"title":"The course of LCMV infection in euthymic and athymic mice pretreated with immunomodulatory agents.","authors":"P Anderlik,&nbsp;I Szeri,&nbsp;Z Bános,&nbsp;Z Barna","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Balb/c (euthymic) and nu/nu (athymic) mice were treated intraperitoneally with TP-4 (a synthetic tetrapeptide, thymopoietin sequence analog) or with Mannozym (1% zymosan suspension), and were infected intracerebrally with LCM virus. Both of the agents contributed to the development of fatal choriomeningitis, consequently stimulated the cellular immune response in euthymic mice, but the athymic mice either treated or not, survived the infection, consequently the agents had no effect on the course of LCM virus infection. Both agents exerted a thymus-dependent cellular immune response stimulating effect. That is, an immunostimulatory effect can be realized only in the presence of the thymus or the T-dependent lymphoid system.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 3-4","pages":"235-9"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12517137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of cell culture and storage conditions on HIV-1 infectivity and fusogenic activity. 细胞培养和储存条件对HIV-1感染和融合活性的影响。
Acta microbiologica Hungarica Pub Date : 1992-01-01
J Ongrádi, L Ceccherini-Nelli, J F Szilágyi, S Specter, M Pistello, H M Laird, M Bendinelli
{"title":"The influence of cell culture and storage conditions on HIV-1 infectivity and fusogenic activity.","authors":"J Ongrádi,&nbsp;L Ceccherini-Nelli,&nbsp;J F Szilágyi,&nbsp;S Specter,&nbsp;M Pistello,&nbsp;H M Laird,&nbsp;M Bendinelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have previously demonstrated that acidic medium inhibits the replication of HIV-1. The present study was designed to examine the effects of other growth conditions and infection of fibroblasts by coculture with HIV infected lymphoid cells. Several lymphoblastoid cell lines normally grown in RPMI-1640 were grown in Eagle's MEM. These cells supported virus replication to higher titres than did RPMI-1640. Peak viral titres were achieved within 24-48 h after newly infected or chronically infected cells were placed in fresh medium. When virus was stored in liquid medium either frozen or at higher temperatures, virus titres were retained for several months while frozen but decreased upon storage at 4 degrees C or higher. If cells were passaged after trypsinization in Ca(++)-depleted medium, then a decreased susceptibility of cells for HIV-1 by 2 log10 at 24 h post infection was observed. Infectivity of cell-free and cell-associated HIV-1 was measured using syncytium formation, reverse transcriptase activity and p24 antigen. No fusion between HIV-1 infected CD4+ lymphoblasts and CD4- fibroblasts was observed but HIV-1 infected lymphoid cells, even in the absence of syncytium formation, exerted a strong toxic effect on fibroblasts. This study extends previous findings that medium acidity was inhibitory to virus replication and survival. Thus, conditions for study of HIV must be well controlled in buffered medium so that misleading results are not obtained regarding virus multiplication and possibly regarding transmission to and pathogenesis in CD4- cells.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 3-4","pages":"207-21"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12459198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacteriocin-like antagonism in Yersinia enterocolitica. 小肠结肠炎耶尔森菌的细菌素样拮抗作用。
Acta microbiologica Hungarica Pub Date : 1992-01-01
K Csiszár, I Tóth
{"title":"Bacteriocin-like antagonism in Yersinia enterocolitica.","authors":"K Csiszár,&nbsp;I Tóth","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 121 Yersinia enterocolitica O3 isolates from patients with gastroenteritis and 37 Y. enterocolitica reference strains with different O antigens were tested for bacteriocine production and sensitivity. By using cross-streaking method strains belonging to serogroups of O5; O7,8; O7,13; O11; O11,23; O13,27; O17; O19,8 and O34 produced bacteriocin-like substances. None of the Y. enterocolitica O3 strains produced bacteriocin-like material and most of them were uniformly sensitive against the bacteriocin-like material produced by strains of serogroups O7,8; O7,13; O13,27 and O19,8. By sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) significant differences were demonstrated in the whole cell protein patterns of Y. enterocolitica reference strains belonging to different serogroups in the range of 33-47 kilodalton (kDa). Out of the ten examined bacteriocin-like material producer strains only one strain harboured a plasmid of about 60 megadalton (MDa).</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 2","pages":"193-201"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12459628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combined effects of flavonoids and acyclovir against herpesviruses in cell cultures. 黄酮类化合物和无环鸟苷在细胞培养中对疱疹病毒的联合作用。
Acta microbiologica Hungarica Pub Date : 1992-01-01
I Mucsi, Z Gyulai, I Béládi
{"title":"Combined effects of flavonoids and acyclovir against herpesviruses in cell cultures.","authors":"I Mucsi,&nbsp;Z Gyulai,&nbsp;I Béládi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The combined antiviral effects of some flavonoid compounds and acycloguanosine (acyclovir, Zovirax) were studied on the multiplication of herpes simplex virus types 1 and 2 in HEp-2 cells and on pseudorabies (Aujeszky) virus in chick embryo fibroblast cells by the yield reduction method. The flavonoids quercetin, quercitrin (quercetin-3-L-rhamnoside) and apigenin exhibit antiviral activity against these herpesviruses, and acyclovir is currently one of the most effective antiherpetic agents. In these studies, the simultaneous application of flavonoids with acyclovir resulted in an enhanced antiviral activity. A mathematical formula was used to interpret the drug interaction, resulting in FIC (fractional inhibitory concentration) indices. Meaning a synergic interaction, all combinations exhibited synergy, FIC values of 0.6-0.8 being commonly observed.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 2","pages":"137-47"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12512281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The mouse ligated intestinal loop assay for the studies on enteroinvasive Escherichia coli. 小鼠结扎肠袢法研究肠侵入性大肠杆菌。
Acta microbiologica Hungarica Pub Date : 1992-01-01
T Sakaguchi, S Sakaguchi, L Nakamura, Y Kudo
{"title":"The mouse ligated intestinal loop assay for the studies on enteroinvasive Escherichia coli.","authors":"T Sakaguchi,&nbsp;S Sakaguchi,&nbsp;L Nakamura,&nbsp;Y Kudo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enteroinvasive Escherichia coli exhibited a positive reaction in the mouse intestinal loop assay except for noninvasive mutant strains. These mean values of fluid weight per gut length of mouse loops inoculated with enteroinvasive E. coli were significantly higher than that given by brain heart infusion broth. Oedema and swelling in all positive loops, increased bacterial cell numbers within intestinal loops were observed.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 3-4","pages":"223-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12517135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological changes of Neisseria gonorrhoeae in amniotic fluid of pregnant women in their midtrimester. 妊娠中期孕妇羊水淋病奈瑟菌的形态变化。
Acta microbiologica Hungarica Pub Date : 1992-01-01
J Péc, M Mlyncek, P Moravcík, K Belej, Z Lazárová
{"title":"Morphological changes of Neisseria gonorrhoeae in amniotic fluid of pregnant women in their midtrimester.","authors":"J Péc,&nbsp;M Mlyncek,&nbsp;P Moravcík,&nbsp;K Belej,&nbsp;Z Lazárová","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ten selected samples of amniotic fluid obtained through transabdominal amniocentesis from 10 pregnant women in their 17th-19th weeks of pregnancy were investigated for the survival of Neisseria gonorrhoeae in amniotic fluid. In 8 cases an antibacterial effect was observed with morphological changes comparable to the effect of benzylpenicillin. When N. gonorrhoeae was inoculated in amniotic fluid at 10(5)-10(7) cells/ml, it survived as an average 2 h longer than after inoculation of 10(2)-10(4) cells/ml. Electron microscopic pictures of gonococci taken after 2.6 and 24 h incubation in amniotic fluid correlated with the growth curves. Electron microscopically there were marked morphologic changes of N. gonorrhoeae, viz. vacuolar degeneration of their cytoplasm with a damage to the bacterial wall up to its complete destruction and lysis of the cell.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 1","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12797858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Legionella pneumophila-specific antibody by indirect immunofluorescence assay. 间接免疫荧光法检测嗜肺军团菌特异性抗体。
Acta microbiologica Hungarica Pub Date : 1992-01-01
M J Casal, M J Linares Sicilia, J Martinez Nebreda, F Solis Cuesta
{"title":"Detection of Legionella pneumophila-specific antibody by indirect immunofluorescence assay.","authors":"M J Casal,&nbsp;M J Linares Sicilia,&nbsp;J Martinez Nebreda,&nbsp;F Solis Cuesta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibodies to Legionella pneumophila were found by indirect immunofluorescence assay in 525 samples of human serum. The samples were obtained from 451 patients who were suspected of having an acute infectious illness, with mainly respiratory symptoms; 90 patients had antibodies to L. pneumophila (19.9%). The results suggest that the prevalence of L. pneumophila is greater than had previously been supposed.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 1","pages":"55-9"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12797862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A 4-kilobase congo red binding plasmid DNA fragment of Shigella dysenteriae 1 suppresses the growth and cell differentiation in Escherichia coli. 志贺氏痢疾杆菌1的4千碱基刚果红结合质粒DNA片段抑制大肠杆菌的生长和细胞分化。
Acta microbiologica Hungarica Pub Date : 1992-01-01
D Biswas, N M Shaikh, A N Ghosh, R Kumar
{"title":"A 4-kilobase congo red binding plasmid DNA fragment of Shigella dysenteriae 1 suppresses the growth and cell differentiation in Escherichia coli.","authors":"D Biswas,&nbsp;N M Shaikh,&nbsp;A N Ghosh,&nbsp;R Kumar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A 4-kilobase congo red binding plasmid DNA fragment of pCAT 120 of Shigella dysenteriae 1 was transferred to an Escherichia coli K12 strain by transformation. Transformants were unable to grow in any liquid broth medium. Electron microscopic studies revealed that the transformants grown on tryptic soy agar were associated in clusters after cell division. Normal cell separation among the transformants in comparison with recipient E. coli K12 was only observed when the growth medium was supplemented with sterile culture filtrate of the recipient strain. An unknown factor(s) required for cell separation located on the chromosome was suppressed by a 4-kb congo red binding plasmid DNA (pCAT 120) fragment of S. dysenteriae 1.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 2","pages":"203-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12482149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shigella-type pathomechanism in the "mouse model". “小鼠模型”中的志贺氏菌型病理机制。
Acta microbiologica Hungarica Pub Date : 1992-01-01
I Kétyi, J Fischer
{"title":"Shigella-type pathomechanism in the \"mouse model\".","authors":"I Kétyi,&nbsp;J Fischer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A strain of Escherichia coli K-12 carrying the 140-Megadalton virulence plasmid of the enteroinvasive E. coli--J53(pSPl)--showed high virulence in the \"mouse model\", in chick embryos, but not in the Serény test. It expresses the outer membrane proteins thermoregulatedly, encoded also by the virulence plasmid. In orally infected streptomycin-pretreated mice this strain infects only the large bowel, shows adherence to the epithelial surface, but in its first step preferentially to the mucus excreted by the goblet cells. Epithelial penetration and intracellular multiplication occurs with a characteristic localization of bacteria in the depth of crypts. Consequence of the infection is degeneration of the epithelial surface, its denudation.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12797861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plasmids encoding for erythromycin ribosomal methylase of Staphylococcus epidermidis and Staphylococcus simulans. 表皮葡萄球菌和拟葡萄球菌红霉素核糖体甲基化酶编码质粒。
Acta microbiologica Hungarica Pub Date : 1992-01-01
I Barcs, L Jánosi
{"title":"Plasmids encoding for erythromycin ribosomal methylase of Staphylococcus epidermidis and Staphylococcus simulans.","authors":"I Barcs,&nbsp;L Jánosi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two 1.7 Md plasmids of Staphylococcus epidermidis and three ones of Staphylococcus simulans determining inducible macrolide-lincosamide resistance are identical as judged by restriction endonuclease fingerprinting. These plasmids designated pEI2101, pEI9105, pE1107, pEI1108 and pEI6104, respectively, belong to the incompatibility group 12. Dot-blot hybridization by photobiotin-labelled gene probe developed from S. aureus erythromycin ribosomal methylase gene showed cross hybridization between methylase-coding reference plasmids and the tested ones. The examined plasmids proved to be no transmissible in mating experiments into S. aureus recipients.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"39 1","pages":"85-92"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12799255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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