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Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology最新文献

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Production of potent antivenin against cobra venom with conjugated-cobrotoxin. 结合眼镜蛇毒素制备抗眼镜蛇毒强效抗蛇毒血清。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-09-01
H C Huang, M Y Liau, Y C Lee
{"title":"Production of potent antivenin against cobra venom with conjugated-cobrotoxin.","authors":"H C Huang,&nbsp;M Y Liau,&nbsp;Y C Lee","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cobra venom (Naja naja atra) and its fractions obtained by ammonium sulfate precipitation were subjected to chromatography on CM-Cellulose colum. A highly purified cobrotoxin obtained by the repeated chromatography on preparative CM-Cellulose column was 6.7 times more toxic than the original cobra venom. The toxin was detoxified by a bifunctional reagent, glutaraldehyde, to about 99.8% and utilized for immunization in animals. Mice received 4 weekly immunization with detoxified cobrotoxin and challenged one week after the last injection showed 60% protection in rabbits by immunization with detoxified cobrotoxin reached 360 LD50 neutralizing level against the cobra venom within 30 days. The results indicate that it is feasible and promising to prepare potent antivenin in animals by glutaraldehyde-treated cobrotoxin.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 3","pages":"93-8"},"PeriodicalIF":0.0,"publicationDate":"1979-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11340795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Studies on staphylococcal enterotoxin B. III. Purification with absorbents]. 葡萄球菌肠毒素b的研究[j]。用吸收剂净化]。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-06-01
C P Chen, N L Wang, F H Tai, J S Chung
{"title":"[Studies on staphylococcal enterotoxin B. III. Purification with absorbents].","authors":"C P Chen,&nbsp;N L Wang,&nbsp;F H Tai,&nbsp;J S Chung","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The biological and immunological activities of Staphylococcal enterotoxin B are stable in pH 2.0 approximately 11.0, and also resistant to proteolytic enzyme (trypsin, pepsin) digestion for 3 approximate 4 hours. Therefore, the toxin could be purified with trypsin digest and then absorbed on kaolin and Kieselgel, followed by eluting the different pH buffer solutions. Further purifications was chromatographied on CM-Sephadex column.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 2","pages":"65-9"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11599182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Site specific mutagenesis (author's transl)]. [位点特异性诱变(作者译)]。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-06-01
C T Chu
{"title":"[Site specific mutagenesis (author's transl)].","authors":"C T Chu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 2","pages":"51-64"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11444920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A trial on nutritionally forced mating of conventional glutamate-producing bacteria]. 传统谷氨酸产生菌的营养强迫交配试验
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-06-01
H H Wang, C Y Hong, J Y Chen, T H Huang
{"title":"[A trial on nutritionally forced mating of conventional glutamate-producing bacteria].","authors":"H H Wang,&nbsp;C Y Hong,&nbsp;J Y Chen,&nbsp;T H Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By nutritionally forced mating, we tried to find the sex factor in glutamate producing bacteria with some auxtrophic mutants of Corynebacterium glutamicum, Micrococcus glutamicus and Brevibacterium divaricatum. We failed to find true mates in this trial, even when the cells cured by acridine orange were used to mate with non-cured ones. There were some positive growth when two densed cell suspensions were dropped on the minimum medium, but after subcultured on the minimum medium no growth was found. It seem likely that the bacteria used here, at least, require an enforced cell adhesion, unless true mating is not feasible. The evidence that Corynebacterium renale has pili might be an interesting reference, but we could not find pili in the bacteria used here.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 2","pages":"70-80"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11788711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Newcastle disease virus in Taiwan: II, Relationship between polykaryocytosis and virus virulence. 台湾新城疫病毒:II，多核性与病毒毒力的关系。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-06-01
W F Chen, Y S Lu, M C Chen
{"title":"Newcastle disease virus in Taiwan: II, Relationship between polykaryocytosis and virus virulence.","authors":"W F Chen,&nbsp;Y S Lu,&nbsp;M C Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fifteen selcted local isolates and five known Newcastle disease virus strains were examined for their cytopathic effects in chick embryo kidney (CEK) cells, egg-infectious units in chick embryos (CE), virulence by mean death time, intracerebral and intravenous pathogenicity indexes for CE and chicks, and ability to cause polykaryocytosis of fusion from within (FFWI) or fusion from without (FFWO) in CEK and BHK-21 monolayer cells. The capacity of the different virus strains to induce cell FFWI at 15 hr post-infection was related to their virulence for CE and chicks, but cell FFWO did not seem to be any relationship with the virulence of the strains.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 2","pages":"43-50"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11786646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Factors contributing to impairment of the mixed lymphocyte reaction in leprosy. 导致麻风病混合淋巴细胞反应受损的因素。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-06-01
S H Han, Z N Chang, L C Tsai, K H Hsu
{"title":"Factors contributing to impairment of the mixed lymphocyte reaction in leprosy.","authors":"S H Han,&nbsp;Z N Chang,&nbsp;L C Tsai,&nbsp;K H Hsu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Whereas the mixed lymphocyte reaction was essentially normal in inactive lepromatous leprosy and tuberculoid leprosy, it was severely impaired in active lepromatous leprosy. The impairment was found to be contributed by certain unknown factors in their plasma and subnormal reactivity of their T lymphocytes. The plasma derived from active lepromatous leprosy patients depressed the reaction of normal cells and normal plasma enhanced the reaction of active lepromatous lymphocytes. The cellular factor was studied by using a one-way reaction in which one of the two lymphocyte preparations was inactivated with mitomycin C. The impairment of blastogenesis of active lepromatous lymphocytes was partially reversed by substituting inactivated normal cells for similarly treated leprous cells, and conversely the response of normal allogeneic lymphocytes was depressed by substituting inactivated leprous lymphocytes as the stimulator cells.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 2","pages":"35-42"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11378263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hepatitis B surface antigen in pregnant women. 孕妇乙型肝炎表面抗原。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-03-01
F J Hsieh, S O Hsieh, K Y Wang
{"title":"Hepatitis B surface antigen in pregnant women.","authors":"F J Hsieh,&nbsp;S O Hsieh,&nbsp;K Y Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Screening of HBsAg in 289 randomly selected normal pregnant women was done with counterelectrophoresis. Ten percent were found to be HBsAg positive. All of the 289 cord blood samples were HBsAg negative. Babies born to the HBsAg positive mothers were followed 2-8 months (average 3.5 months) after delivery and 37.5% were positive for HBsAg. Accordingly, the vertical transmission of HBsAg was postulated to occur during labor or after birth.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11786641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of a cellulase producing mold, Trichoderma sp. W-10 isolated from infected mushroom spawn. 从受感染菌种中分离的产纤维素酶霉菌Trichoderma sp. W-10的特性。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-03-01
C F Lin
{"title":"Characterization of a cellulase producing mold, Trichoderma sp. W-10 isolated from infected mushroom spawn.","authors":"C F Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A potent cellulase producing mold, Tichoderma sp. W-10 suitable for enzyme production with solid state culture was isolated from infected mushroom spawn, its characteristics and taxonomic studies were carried out. As a result, this mold was identified as Trichoderma koningii (Oudemans) according to the keys of Gilman and Rifai's classification system and the morphological features described by Komatsu. Meanwhile, a detail survey of physiological, cultural and biochemical characteristics were also described.</p>","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 1","pages":"6-12"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11786645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Protozan and helminthic infections among the government workers and students of Miao-Li District in Miao-Li County: a highly endemic area of clonorchiasis in Taiwan (author's transl)]. 台湾省支睾吸虫病高流行区苗丽县政府工作人员和学生的原虫和寄生虫感染(作者译)。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-03-01
S J Ong, S C Lu
{"title":"[Protozan and helminthic infections among the government workers and students of Miao-Li District in Miao-Li County: a highly endemic area of clonorchiasis in Taiwan (author's transl)].","authors":"S J Ong,&nbsp;S C Lu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 1","pages":"13-20"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11786642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Bi-weekly treatments of Enterobius infections with pyrantel pamoate (author's transl)]. [双周用帕莫酸吡喃酯治疗肠杆菌感染[作者简介]。
Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology Pub Date : 1979-03-01
H H Lee
{"title":"[Bi-weekly treatments of Enterobius infections with pyrantel pamoate (author's transl)].","authors":"H H Lee","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76873,"journal":{"name":"Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology","volume":"12 1","pages":"21-6"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11786643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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