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Zeitschrift fur Parasitenkunde (Berlin, Germany)最新文献

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A simple method for cloning leishmanial promastigotes. 克隆利什曼原虫的一种简易方法。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00925476
D A Evans, V Smith
{"title":"A simple method for cloning leishmanial promastigotes.","authors":"D A Evans,&nbsp;V Smith","doi":"10.1007/BF00925476","DOIUrl":"https://doi.org/10.1007/BF00925476","url":null,"abstract":"<p><p>A method of cloning leishmanial promastigotes is described in which mid-exponential phase cultures are diluted to contain approximately 3 X 10(3) promastigotes per ml. Hanging-drop preparations made from 0.2-0.4 microliter volumes of the diluted culture seen to contain a single promastigote are picked-up in glass capillary tubes. Additional culture medium is taken into the capillaries which are then heat-sealed and incubated at 22 degrees C. Growth of leishmanial promastigotes inside the sealed capillary tubes is followed by direct microscopic observation through the walls of the tubes. When active promastigotes are seen the contents of the tubes are inoculated into small volumes of culture medium. The method is extremely easy to use, requires no specialised apparatus, and has been successfully used with different strains and species of Leishmania, with up to 100 percent of the cloned organisms growing.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 5","pages":"573-6"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14893486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Naegleria fowleri and N. lovaniensis: differences in sensitivity to trimethoprim and other antifolates. 福氏奈格氏菌和洛瓦尼奈格氏菌:对甲氧苄啶和其他抗叶酸药物敏感性的差异。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00925478
L Cerva
{"title":"Naegleria fowleri and N. lovaniensis: differences in sensitivity to trimethoprim and other antifolates.","authors":"L Cerva","doi":"10.1007/BF00925478","DOIUrl":"https://doi.org/10.1007/BF00925478","url":null,"abstract":"<p><p>The growth of Naegleria fowleri cultures in a BCS medium was not affected either by trimethoprim at 400 micrograms/ml or by aminopterine, 3,5-diaminopterine and methotrexate at 500 micrograms/ml. N. lovaniensis propagation in the same medium was inhibited with 10 micrograms/ml of trimethoprim, 50 micrograms/ml methotrexate and 100 micrograms/ml 3,5-diaminopteridine. Aminopterine was ineffective at a concentration of 500 micrograms/ml. The inhibitory effect of trimethoprim on N. lovaniensis cultures depended on the medium composition and could be neutralized by an addition of folic or tetrahydrofolic acids and a suspension of heat-killed Enterobacter aerogenes. Thymine, thymidine, hypoxantine and 2-amino-4-hydroxy-6-(tetrahydroxybutyl)-pteridine did not have an adverse effect. Trimethoprim activity in N. fowleri cultures could not be enhanced by the addition of Triton X-100 and Polymyxine B. Cryolyzate of N. fowleri amoebae did not influence the trimethoprim inhibition of N. lovaniensis cultures. Deviation in dihydrofolatereductase chemical structure or thymine dependency seems to be the probable explanation for N. fowleri antifolate resistance.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 5","pages":"585-90"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14893487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scanning electron microscopy and chemical excystation of Fascioloides magna (Trematoda) metacercariae. 大片形吸虫(吸虫)囊蚴的扫描电镜和化学清除。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00925484
B Fried, T S Vates, N Wisnewski, B E Stromberg
{"title":"Scanning electron microscopy and chemical excystation of Fascioloides magna (Trematoda) metacercariae.","authors":"B Fried,&nbsp;T S Vates,&nbsp;N Wisnewski,&nbsp;B E Stromberg","doi":"10.1007/BF00925484","DOIUrl":"https://doi.org/10.1007/BF00925484","url":null,"abstract":"<p><p>Scanning electron microscopy was used to study encysted metacercariae and newly excysted juveniles of Fascioloides magna. The outer cyst was rough, coarse and discontinuous in the ventral aspect; the inner cyst was smooth. The newly excysted metacercaria was plump and contained numerous tegumentary spines; large dome-shaped papillae were prominent around the oral sucker and on the rim of the acetabulum. Encysted metacercariae with outer cysts were excysted in an alkaline bile salts-trypsin medium at an elevated temperature in the absence of acid saline or acid pepsin pretreatment. Pretreatment in acid saline slightly decreased subsequent excystation, while pretreatment in acid pepsin slightly enhanced subsequent excystation in the alkaline bile salts-trypsin medium.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 5","pages":"631-4"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925484","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14893488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A biometric study of Onchocerca volvulus microfilariae from Nigeria using the nuclear counting method. 尼日利亚盘尾丝虫微丝虫的核计数生物特征研究。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00925105
T Mimori, I Tada, K Shiwaku, G O Ufomadu, B E Nwoke
{"title":"A biometric study of Onchocerca volvulus microfilariae from Nigeria using the nuclear counting method.","authors":"T Mimori,&nbsp;I Tada,&nbsp;K Shiwaku,&nbsp;G O Ufomadu,&nbsp;B E Nwoke","doi":"10.1007/BF00925105","DOIUrl":"https://doi.org/10.1007/BF00925105","url":null,"abstract":"","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 6","pages":"835-6"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14916798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The effect of precocene II and 20-OH-ecdysone on Litomosoides carinii and Dipetalonema viteae in vivo. 早熟II和20- oh -蜕皮素对石首虫和石首虫体内的影响。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00925107
H Mehlhorn, K D Spindler, M Spindler-Barth, V Walldorf, P Andrews, H Thomas
{"title":"The effect of precocene II and 20-OH-ecdysone on Litomosoides carinii and Dipetalonema viteae in vivo.","authors":"H Mehlhorn,&nbsp;K D Spindler,&nbsp;M Spindler-Barth,&nbsp;V Walldorf,&nbsp;P Andrews,&nbsp;H Thomas","doi":"10.1007/BF00925107","DOIUrl":"https://doi.org/10.1007/BF00925107","url":null,"abstract":"","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 6","pages":"843-5"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14916800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Evidence of the growth factor in mouse serum infected with Spirometra erinacei plerocercoids. 小鼠血清中生长因子感染羊角螺蚴的证据。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00927738
K Shiwaku, K Hirai, M Torii, T Tsuboi
{"title":"Evidence of the growth factor in mouse serum infected with Spirometra erinacei plerocercoids.","authors":"K Shiwaku,&nbsp;K Hirai,&nbsp;M Torii,&nbsp;T Tsuboi","doi":"10.1007/BF00927738","DOIUrl":"https://doi.org/10.1007/BF00927738","url":null,"abstract":"<p><p>To investigate the action of the growth factor secreted by Spirometra erinacei plerocercoids, various organ weights, body weight and head-body length were measured in Snell normal and dwarf mice after injection with the serum from mice and rats. Serum from mice infected with the plerocercoids caused significant increases in the weights of the liver and spleen, in the same manner as mice infected with the plerocercoids. However, serum from rats infected with plerocercoids did not cause significant changes in these parameters. The growth factor in the serum of mice infected with plerocercoids was stable at -20 degrees C for at least 6 months and easily passed through the peritoneum.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 1","pages":"83-7"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00927738","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15075904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Comparative analysis of the Schistosoma mansoni and Schistosoma bovis cercarial production under the influence of praziquantel. 吡喹酮对曼氏血吸虫和牛血吸虫产虫量影响的比较分析。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00928745
R Touassem, C Combes
{"title":"Comparative analysis of the Schistosoma mansoni and Schistosoma bovis cercarial production under the influence of praziquantel.","authors":"R Touassem,&nbsp;C Combes","doi":"10.1007/BF00928745","DOIUrl":"https://doi.org/10.1007/BF00928745","url":null,"abstract":"<p><p>Snails infected with Schistosoma mansoni and S. bovis and fed with a food-Praziquantel mixture stop shedding cercariae for several days. The cercarial production restarts at different levels after stopping treatment: for some Biomphalaria glabrata, the restarting phase of production reaches a high level whereas for other B. glabrata and all of the P. metidjensis, the level of production remains low. Histological studies revealed that at the exact moment of this treatment, there is a total destruction of many nearly mature cercariae whereas the young cercarial embryos and the tegument of the sporocyst remain unharmed. When treatment stops, there is a pronounced proliferation of third-generation sporocysts (Sp III) which invade the available space of the snails' digestive gland.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 3","pages":"345-51"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00928745","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14836151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Serum protein from Leishmania brasiliensis infected hamster that suppress lymphocyte response of normal hamster lymphocytes. 巴西利什曼原虫感染的仓鼠血清蛋白抑制正常仓鼠淋巴细胞的淋巴细胞反应。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00928738
J A O'Daly, Z Cabrera
{"title":"Serum protein from Leishmania brasiliensis infected hamster that suppress lymphocyte response of normal hamster lymphocytes.","authors":"J A O'Daly,&nbsp;Z Cabrera","doi":"10.1007/BF00928738","DOIUrl":"https://doi.org/10.1007/BF00928738","url":null,"abstract":"<p><p>Immunosuppression in Leishmania brasiliensis (LB) or L. donovani (LD) infected hamsters is correlated with the appearance of two serum protein bands found at 21, 60, 68 and 76 days post LB-infection and with eight bands at 21 days post-LD-infection probably of host origin. A protein band from LB-infected hamster serum isolated by electrofocusing, suppressed the blastogenic response of normal lymphocytes to T and B cell mitogens.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 3","pages":"293-8"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00928738","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14836238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathogenesis of lymphatic filariasis in man. 人淋巴丝虫病的发病机制。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00927731
D H Connor, J R Palmieri, D W Gibson
{"title":"Pathogenesis of lymphatic filariasis in man.","authors":"D H Connor,&nbsp;J R Palmieri,&nbsp;D W Gibson","doi":"10.1007/BF00927731","DOIUrl":"https://doi.org/10.1007/BF00927731","url":null,"abstract":"","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 1","pages":"13-28"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00927731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14638370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Migration of two Egyptian strains of Schistosoma mansoni in hamsters. 两株埃及曼氏血吸虫在仓鼠中的迁移。
Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1986-01-01 DOI: 10.1007/BF00925108
N S Mansour, G N Soliman, F M el Assal, K Garo
{"title":"Migration of two Egyptian strains of Schistosoma mansoni in hamsters.","authors":"N S Mansour,&nbsp;G N Soliman,&nbsp;F M el Assal,&nbsp;K Garo","doi":"10.1007/BF00925108","DOIUrl":"https://doi.org/10.1007/BF00925108","url":null,"abstract":"","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 6","pages":"847-50"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14231514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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