{"title":"The effect of Helisoma duryi on the cercarial production of Schistosoma mansoni-infected Biomphalaria alexandrina: evaluation of chemical interferences and direct competition.","authors":"H Madsen","doi":"10.1007/BF00932920","DOIUrl":"https://doi.org/10.1007/BF00932920","url":null,"abstract":"<p><p>The effect of Helisoma duryi or uninfected Biomphalaria alexandrina conditioning of water, as well as the effect of direct competition with these, on the total cercarial production, growth and survival of Schistosoma mansoni-infected B. alexandrina was investigated. The total cercarial production varied considerably between experimental groups. No differences were found between groups maintained in water-conditioned by H. duryi or by uninfected B. alexandrina with regard to the total cercarial production, growth and survival. The cercarial production was correlated with snail size, but showed no differences between the two treatments. Direct competition with H. duryi resulted in reduced cercarial production and growth of infected B. alexandrina as compared with snails in competition with uninfected conspecifics.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 1","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00932920","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15097004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Solubilization and partial purification of a cell surface component of Leishmania braziliensis.","authors":"J A Misle, M E Márquez, A G Hernández","doi":"10.1007/BF00928344","DOIUrl":"https://doi.org/10.1007/BF00928344","url":null,"abstract":"<p><p>A simple procedure that allowed the extraction and partial purification of a component of 65,000 mol.wt. from the surface of Leishmania braziliensis promastigotes is described. Iodinated cell surface membrane fractions were solubilized using Triton X-100 followed by Nonidet P-40. The macromolecular components were then freed of the detergents by passage of the extracts through a column of DE52 cellulose. The component of 65,000 mol.wt. was eluted from the column with 1 m NaCl. This component in whole parasites was immunoprecipitated by sera from patients with cutaneous, mucocutaneous, and diffuse cutaneous leishmaniasis and kala-azar. None of the major surface determinants reacted with sera from normal individuals with antibovine rabbit serum. The relevance and the possible applications on the immunoprophylaxis of the disease are briefly discussed.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 4","pages":"419-28"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00928344","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15137306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association between intracellular rickettsial-like infections of midgut cells and susceptibility to trypanosome infection in Glossina spp.","authors":"I Maudlin, D S Ellis","doi":"10.1007/BF00925601","DOIUrl":"https://doi.org/10.1007/BF00925601","url":null,"abstract":"","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 5","pages":"683-7"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925601","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15162659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Interaction of aminoglycosides and ionophores in the killing of Crithidia fasciculata.","authors":"M A Millgram, C L Greenblatt, J Shlomai","doi":"10.1007/BF00926795","DOIUrl":"https://doi.org/10.1007/BF00926795","url":null,"abstract":"<p><p>Crithidia fasciculata was utilized as a prescreen to determine the antiprotozoal action of aminoglycoside antibiotics alone and in combination with surface-altering agents. Paromomycin was tested with the carrier ionophores nigericin and valinomycin, the channel ionophore gramicidin and the polyene antibiotics amphotericin B and nystatin. After exposure to the drugs in suspension, organisms were plated out to determine the survival of C. fasciculata. Killing was time dependent for both the antibiotic and the ionophore. Paromomycin action was found to be potentiated by all the surface altering agents. The aminoglycosides kanamycin, gentamycin and streptomycin were studied alone and in combination with nigericin. Synergistic effects were demonstrated both with kanamycin and gentamycin in combination with nigericin. Streptomycin was ineffective both alone and with surface-altering agents.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 6","pages":"699-704"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00926795","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15194566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Action of diethylcarbamazine citrate on protective immunity in rats infected with Nippostrongylus brasiliensis.","authors":"J C Katiyar, S Gupta, A B Sen","doi":"10.1007/BF00928342","DOIUrl":"https://doi.org/10.1007/BF00928342","url":null,"abstract":"<p><p>Rats made immune to Nippostrongylus brasiliensis and treated with diethylcarbamazine citrate (DEC) orally (250 mg/kg X 6) exhibited significant suppression of functional immunity. Similarly, administration of compound 48/80 (100 micrograms/rat i.p.) made the immune rats susceptible to challenge infection. Treatment of rats, with 22-day infection with compound 48/80, histamine (20 mg/rat, per os), or L-histidine (20 mg/rat, orally s.c.) did not accelerate worm expulsion. A massive complement-dependent adherence of peritoneal cells (1 X 10(8], isolated from immune DEC-treated and untreated rats, to infective larvae (L3) was observed. Likewise, heavy congregation of normal peritoneal cells to larvae was noticed when the cells were incubated with sera obtained from immune, DEC-treated or untreated rats. The rats receiving mesenteric lymph node cells (125 X 10(6) i.v.) or sera (0.5 ml or 1 ml X 3 i.p.), obtained from immune DEC-treated rats and challenged with infective larvae developed 50% more worms than those which received cells or serum from untreated immune donors. DEC appears to cause suppression of functional immunity and worm expulsion is not histamine mediated.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 3","pages":"401-8"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00928342","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15125305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunity to Litomosoides carinii in Mastomys natalensis. I. Effect of immunization with microfilariae and existing primary infections on the parasitaemia after microfilariae injection and challenge infection.","authors":"H Zahner, P H Wegerhof","doi":"10.1007/BF00925591","DOIUrl":"https://doi.org/10.1007/BF00925591","url":null,"abstract":"<p><p>Subcutaneous injections of intrauterine stages of Litomosoides carinii into Mastomys natalensis induced strong immunity to i.v. injected blood microfilariae. Immunity, developed after boostering with an i.p. and an i.v. injection of microfilariae, did not totally suppress the parasitaemia of a challenge infection but reduced significantly the microfilaraemia level. No effect was found on number and size of the worms of the challenge infection, the number of microfilariae or the number of leucocytes in the pleural cavity. Delayed type hypersensitivity reactions in challenged animals were similar to those in non-immunized, infected controls. Sera of immunized animals agglutinated microfilariae and mediated cell attachment to microfilariae. Challenge infections did not change this until the end of the fourth week post infection but sera taken 32 days after challenge and later failed to induce such reactions. Challenge infections performed 120 or 240 days after a primary infection did not increase the parasitaemia of recipients. Dissections carried out 130 days after the challenge showed that (a) the developmental rate of the challenge infection was reduced by about 50%; (b) the size of the challenge parasites was reduced; and (c) that these worms produced significantly less embryonic stages in comparison to worms of primary infections, of which about 90% were abnormal.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 5","pages":"583-93"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925591","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15162035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunofluorescent localization of Schistosoma mansoni circulating cathodic antigen in tissues of infected mice using monoclonal antibody.","authors":"A M Deelder, I el-Dosoky, E A Van Marck, Z L Qian","doi":"10.1007/BF00928334","DOIUrl":"https://doi.org/10.1007/BF00928334","url":null,"abstract":"<p><p>In the present study the kinetics of the uptake and deposition of the major circulating cathodic antigen (CCA) of Schistosoma mansoni in liver, spleen, and kidney of S. mansoni infected Swiss mice was investigated in relation to the duration of infection and infection dose (50, 100, 200 cercariae). The presence of antigen was studied with a direct immunofluorescence reaction on frozen sections of the mouse organs, using a fluorescein isothiocyanate (FITC)-labelled mouse IgM monoclonal antibody recognizing a repeating epitope of CCA. CCA was demonstrable from 2 weeks post infection (p.i.) onwards in Kupffer cells in the liver, from 3-4 weeks p.i. onwards in macrophages in the marginal zones in the spleen and from 8 weeks p.i. onwards in kidney glomeruli. The immunofluorescence reactions on CCA in kidney glomeruli, however, remained relatively weak.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 3","pages":"317-23"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00928334","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15011025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Agarwal, J K Saxena, J C Katiyar, S Ghatak, R D Walter
{"title":"Nippostrongylus brasiliensis: occurrence of multiple protein kinases.","authors":"A Agarwal, J K Saxena, J C Katiyar, S Ghatak, R D Walter","doi":"10.1007/BF00926276","DOIUrl":"https://doi.org/10.1007/BF00926276","url":null,"abstract":"<p><p>The presence of cyclic AMP-dependent protein kinase and phosvitin kinases, with activity independent of cyclic nucleotides, was shown in the intestinal nematode Nippostrongylus brasiliensis. The activity of the cyclic AMP-dependent protein kinase was found to be enhanced about 8-fold in the presence of 10(-7) M cyclic AMP; the apparent Km values were determined to be 20 microM and 80 microM for ATP and kemptide, respectively. The molecular weight of the holoenzyme was about 170 000. Two phosvitin kinases could be isolated and distinguished by their molecular weights of 600 000 and 40 000. The activity of the high-molecular-weight phosvitin kinase was effectively inhibited by suramin and heparin. The apparent Km values were found to be 30 microM and 0.1 mg/ml for ATP and phosvitin, respectively. In the case of the low-molecular-weight phosvitin kinase the apparent Km values for ATP and phosvitin were found to be 30 microM and 0.6 mg/ml, respectively. The investigation of different developmental stages of N. brasiliensis revealed a marked higher level of protein kinase activity in the L4 larvae compared to L3 larvae and adults.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 2","pages":"259-64"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00926276","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15108611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunocytochemical localization of gut-associated circulating anodic antigen in Schistosoma japonicum.","authors":"T Fujino, M Hirata, Y Ishii, H Tsutsumi","doi":"10.1007/BF00926799","DOIUrl":"https://doi.org/10.1007/BF00926799","url":null,"abstract":"<p><p>The localization of the gut-associated circulating anodic antigen in Schistosoma japonicum adults was revealed by means of the immunofluorescence and a peroxidase-anti-peroxidase method with the electron microscope. The reaction sites were confined to amorphous material in the cecal lumen. The cecal lumen generally was infolded with lamellae. The antigenic material appeared to be secreted by the rough endoplasmic reticulum, probably through the Golgi apparatus, into the lumen. Observations of male and female worms showed that there was a clear difference between the sexes in antigen concentration. The thick epithelium of the female worm, with well-developed cisternae on the endoplasmic reticulum, produced a lot more antigen than the male. Positive staining with ruthenium red confirmed that the antigenic material was a negatively charged polysaccharide as had been previously reported.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"71 6","pages":"739-45"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00926799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13563412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}