Zeitschrift fur Parasitenkunde (Berlin, Germany)最新文献

{"title":"Trypanosoma cruzi: inhibition of host cell uptake of infective bloodstream forms by alpha-2-macroglobulin.","authors":"T C de Araujo-Jorge,&nbsp;E P Sampaio,&nbsp;W de Souza","doi":"10.1007/BF00928742","DOIUrl":"https://doi.org/10.1007/BF00928742","url":null,"abstract":"<p><p>The infection of murine macrophages and fibroblasts by recently isolated infective bloodstream trypomastigotes of Trypanosoma cruzi is inhibited by the addition of human plasma protease inhibitor alpha-2-macroglobulin (alpha 2M) or of soybean trypsin inhibitor. The ingestion of the non-infective epimastigotes by macrophages is not affected by the physiological protease inhibitor. Incubation of bloodstream trypomastigotes for 20 h in a serum-free axenic medium enhances their ability to infect macrophages in a process influenced by the temperature and sensitive to alpha 2M. After this period the infectivity of the parasites to cells was not sensitive to alpha 2M. These observations suggest that proteases located on the surface and/or secreted by the bloodstream trypomastigote form of T. cruzi may modulate its ability to infect host cells.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 3","pages":"323-9"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00928742","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13570854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of Trypanosoma cruzi with macrophages: effect of previous incubation of the parasites or the host cells with lectins.","authors":"T C de Araújo-Jorge,&nbsp;W de Souza","doi":"10.1007/BF00931143","DOIUrl":"https://doi.org/10.1007/BF00931143","url":null,"abstract":"<p><p>The effect of incubation with lectins of the macrophages or two evolutive stages of Trypanosoma cruzi (noninfective epimastigotes and infective trypomastigotes) on the ingestion of the parasites by mouse peritoneal macrophages was studied. Lectins which bind to residues of mannose (Lens culinaris, LCA), N-acetyl-D-glucosamine or N-acetylneuraminic acid (Triticum vulgaris, WGA), beta-D-galactose (Ricinus communis, RCA), N-acetyl-D-galactosamine (Phaseolus vulgaris, PHA; Dolichos biflorus, DBA; and Wistaria floribunda, WFA), fucose (Lotus tetragonolobus, LTA), and N-acetylneuraminic acid (Limulus polyphemus, LPA) were used. By lectin blockage we concluded that, alpha-D-mannose-like, beta-D-galactose and N-acetyl-D-galactosamine (PHA, reagent) residues, located on the macrophage's surface are required for both epi- and trypomastigote uptake, while N-acetylneuraminic acid and fucose residues, impede trypomastigote ingestion but do not interfere with epimastigote interiorization. Macrophages' N-acetyl-D-glucosamine residues are required for epimastigote uptake. On the other hand, from the T. cruzi surface, mannose residues prevent ingestion of epi- and trypomastigotes. Galactose residues participate in endocytosis of trypomastigotes, but hinder epimastigote interiorization. Exposed N-acetyl-D-glucosamine residues are required for uptake of the two evolutive forms. N-acetylneuraminic acid residues on the trypomastigote membrane prevent their endocytosis by macrophages. These results together with those reported previously showing the effect of monosaccharides on the T. cruzi-macrophage interaction, indicate that (a) sugar residues located on the parasite and on macrophage surface play some role in the process of recognition of T. cruzi, (b) different macrophage carbohydrate-containing receptors are involved in the recognition of epimastigotes and trypomastigotes forms of T. cruzi, (c) N-acetylneuraminic acid residues located on the surface of trypomastigotes or macrophages impede the interaction of the parasite with these host cells, and suggest that (d) sugar-binding proteins located on the macrophage surface participate in the recognition of beta-D-galactose and N-acetyl-D-galactosamine residues located on the surface of trypomastigotes and exposed after blockage or splitting off of N-acetylneuraminic acid residues. Some lectins which bind to macrophages and block the ingestion of parasites did not interfere with their adhesion.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 2","pages":"153-71"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00931143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14216982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural localization of the circulating cathodic antigen in the digestive tract of various life-cycle stages of Schistosoma mansoni.","authors":"R de Water,&nbsp;J A Fransen,&nbsp;A M Deelder","doi":"10.1007/BF00925485","DOIUrl":"https://doi.org/10.1007/BF00925485","url":null,"abstract":"<p><p>In the present study the ultrastructural localization of the schistosome-derived circulating cathodic antigen (CCA) within the digestive tract of various life-cycle stages of the blood fluke Schistosoma mansoni has been determined. Use was made of CCA-specific monoclonal antibodies in a two-step gold-labeling procedure. The following results were obtained: In cercariae gold label was found in the cytoplasm and in the luminal surface coat of the gut epithelium. The oesophagus of this life-cycle stage of the parasite showed minimal CCA reactivity, as gold label was limited to the luminal surface coat, locating proximally to the gut. In 3 1/2-week-old worms and in adult male and female worms CCA was demonstrable in the Golgi apparatus, in cytoplasmic vesicles and in the luminal surface coat; in the caudal quarter of the gut of adult worms CCA was also present in lysosome-like bodies. The oesophagus of these worm preparations did not show any CCA reactivity. In miracidia CCA was not present.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 5","pages":"635-46"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14657094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"14C-desferrioxamine B: uptake into erythrocytes infected with Plasmodium falciparum.","authors":"G Fritsch,&nbsp;A Jung","doi":"10.1007/BF00925092","DOIUrl":"https://doi.org/10.1007/BF00925092","url":null,"abstract":"<p><p>Plasmodium falciparum-infected human erythrocytes (early trophozoite stages) and non-infected erythrocytes were incubated in 1.7 mM 14C-desferrioxamine B (specific activity 1 microCi/2.6 mg desferrioxamine B). After 270 min the cells were washed and the radioactivity was measured in the cell pellet and, after lysis, in cytoplasm and membranes. The results indicate that Desferrioxamine B can the red blood cell and pass through the parasite membrane and that the parasites are killed by the intracellular action of the chelator.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 6","pages":"709-13"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14663962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Giardia lamblia: identification of different strains from man.","authors":"S H Korman,&nbsp;S M Le Blancq,&nbsp;D T Spira,&nbsp;J el On,&nbsp;R M Reifen,&nbsp;R J Deckelbaum","doi":"10.1007/BF00931144","DOIUrl":"https://doi.org/10.1007/BF00931144","url":null,"abstract":"<p><p>Four axenically cultured human Giardia lamblia isolates from Jerusalem (KC-1, 2, 3 and 4) and one from Bethesda (WB) were compared. Three distinct groups were defined by agglutination response to rabbit anti-G. lamblia sera viz. WB; KC-3; and KC-1, 2 and 4. The same major groups were identified by isoenzyme analysis using thin-layer starch-gel electrophoresis, each group differing from the others in three or more of five enzymes studied. In addition, a single enzyme difference distinguished KC-2 from KC-1 and 4. These findings reveal significant heterogeneity in G. lamblia isolates both from widely separated areas and within a single region. Immunoassays for diagnosis of giardiasis should take into account the differences between strains. Heterogeneity among G. lamblia strains may explain the variable clinical manifestations, host response and treatment efficacy characteristic of human giardiasis.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 2","pages":"173-80"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00931144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14825324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine structure of adult Litomosoides carinii (Nematoda: Filarioidea).","authors":"M Franz,&nbsp;P Andrews","doi":"10.1007/BF00927897","DOIUrl":"https://doi.org/10.1007/BF00927897","url":null,"abstract":"<p><p>The fine structure of the body wall and the intestine of male and female Litomosoides carinii was studied in sections through the middle and posterior regions of the worms. In the sublateral cells of the female hypodermis the organelles are arranged in regular layers. The zone with the basal labyrinth is the most prominent layer. The intestinal epithelium of the female worm varies considerably in thickness. The cytoplasm of these cells contains many large droplets. The lateral hypodermal chords of the male worm are very narrow bands. The muscle cells of the male worm are of the circomyarian type, without an afibrillar portion. The intestinal lumen of the male worm is very narrow, and the epithelial cells contain very large droplets. In both sexes the hypodermis contains bacteria. Larger glycogen deposits were absent in the hypodermal chords and in the muscle cells of the midbody regions.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 4","pages":"537-47"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00927897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14871035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental toxocariasis and hyperactivity in mice.","authors":"J Hay,&nbsp;M A Arnott,&nbsp;P P Aitken,&nbsp;A T Kendall","doi":"10.1007/BF00927742","DOIUrl":"https://doi.org/10.1007/BF00927742","url":null,"abstract":"<p><p>An observational study using videorecordings and computer-assisted data analysis was undertaken in order to investigate the behaviour of mice infected with larvae of Toxocara canis. The findings indicated that the infection had a marked effect on five readily and reliably differentiable categories of murine behaviour. A marked increase in the number of shorter bouts of each of the five behaviours was also associated with the infection. These results support previous findings and further suggest that T. canis infection affects the way in which mice respond to their environment. In particular the infection appears to be associated with hyperactivity in mice. Possible causes of such behavioural abnormalities as well as implications of these findings for clinical studies concerned with relationships between T. canis infection and hyperactivity in children are discussed.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 1","pages":"115-20"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00927742","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14872977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of specific antigens from Angiostrongylus cantonensis. 2. By affinity chromatography.","authors":"W W Kum,&nbsp;R C Ko","doi":"10.1007/BF00927894","DOIUrl":"https://doi.org/10.1007/BF00927894","url":null,"abstract":"<p><p>The crude adult worm extract of Angiostrongylus cantonensis was subjected to a series of affinity chromatography for selective removal of host antigens as well as cross-reactive components with other helminths. The purified fraction designated as AC(p) with molecular weights ranging from 10,000-42,000 was found to contain antigenic components specific to A. cantonensis as determined by immunoelectrophoresis and enzyme-linked immunosorbent assay.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 4","pages":"511-6"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00927894","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13574208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The distribution of inhibited early third stage Cyathostominae larvae in the large intestine of the horse.","authors":"M Eysker,&nbsp;M H Mirck","doi":"10.1007/BF00925101","DOIUrl":"https://doi.org/10.1007/BF00925101","url":null,"abstract":"<p><p>The distribution of inhibited early third stage Cyathostominae larvae in different parts of the large intestine of the horse was studied in 20 Shetland ponies necropsied in autumn 1982, 1983 and 1984. The location of the larvae in the large intestinal wall was studied by histological examination of the intestines of the eight ponies from 1984. Inhibited larvae were located predominantly and more or less equally in the caecum and the ventral colon. Generally fewer early L3 were in the dorsal colon. In 1984 a considerable proportion (mean 17%, range 9.7-36.9%) of the inhibited larvae was found in the contents instead of in the mucosa of the large intestine, despite a housing period under helminth free conditions of 5 weeks. These larvae probably had been overlooked in 1982 and 1983. In sections cut from the intestinal wall most early L3 were found in the lamina propria. They were surrounded by a small fibrous capsule. Some were found in the lumen or epithelium of the Lieberkühn's crypts and a small proportion in the submucosa.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 6","pages":"815-20"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00925101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14916795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced development of Plasmodium falciparum in beta-thalassaemic erythrocytes.","authors":"R Kaminsky,&nbsp;N Krüger,&nbsp;E Hempelmann,&nbsp;W Bommer","doi":"10.1007/BF00927899","DOIUrl":"https://doi.org/10.1007/BF00927899","url":null,"abstract":"","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 4","pages":"553-6"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00927899","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14652266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}