Thymus最新文献_第7页

Thymus histogenesis in the lizard, Chalcides ocellatus. 壁虎胸腺的组织发生。

Thymus Pub Date : 1993-09-01

A H Saad, S el-Deeb, M Mobarez, M A Soliman

引用次数: 0

The role of peptide in the positive selection of CD8+ T cells in the thymus. 肽在胸腺CD8+ T细胞阳性选择中的作用。

Thymus Pub Date : 1993-09-01

P G Ashton-Rickardt

引用次数: 0

Untransfected and SV40-transfected fetal and postnatal human thymic stromal cells. Analysis of phenotype, cytokine gene expression and cytokine production. 未转染和转染sv40的胎儿和出生后人胸腺基质细胞。表型、细胞因子基因表达及细胞因子产生分析。

Thymus Pub Date : 1993-08-01

A H Galy, R de Waal Malefyt, A Barcena, S M Peterson, H Spits

{"title":"Untransfected and SV40-transfected fetal and postnatal human thymic stromal cells. Analysis of phenotype, cytokine gene expression and cytokine production.","authors":"A H Galy, R de Waal Malefyt, A Barcena, S M Peterson, H Spits","doi":"","DOIUrl":"","url":null,"abstract":"The thymic stromal network is complex and heterogeneous, containing thymic epithelial cells which are thought to play an important role during T-cell development and thymic fibroblasts which role is less defined. We herein present a phenotypic and functional comparison between defined thymic stromal cell populations. We transfected SV40 ori- into fetal and postnatal thymic stromal cell cultures and obtained SV40-immortalized clones of epithelial and fibroblastic nature as demonstrated by expression of intracellular keratin. These various clones were characterized in detail and compared to their untransfected bulk culture counterparts for phenotype, cytokine gene expression and cytokine production. All the different thymic stromal cells examined, constitutively expressed ICAM-1, LFA-3, MHC class I antigens, CD44, and the genes coding for IL-7, SCF and TGF-beta, but not TNF-alpha. After IL-1 stimulation, epithelial cells seemed to produce more GM-CSF than fibroblasts, and that trend was also seen for IL-6 secretion. SV40 cells were also regulated by IFN-gamma which induced MHC class II antigens and inhibited the IL-1 induced GM-CSF production. SV40 cells differed from their untransfected counterparts by an atypical expression of CD40 and lacked constitutive IL-1 alpha gene expression. We isolated clones with distinct properties, 24SV48, a highly proliferative CD34 positive TEC secreting low levels of GM-CSF and lacking constitutive IL-1 alpha and beta gene expression, and CT1SV93, an epithelial clone of postnatal origin with a high IL-1-induced cytokine production. In spite of differences with untransfected bulk cultures, the various SV40 immortalized clones may represent useful tools to further study the human thymic stroma.","PeriodicalId":76738,"journal":{"name":"Thymus","volume":"22 1","pages":"13-33"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18515421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural study of macrophages of the rat thymus after cyclosporin treatment. 环孢素对大鼠胸腺巨噬细胞超微结构的影响。

Thymus Pub Date : 1993-08-01

N M Milićević, Z Milićević, M Colić, E W Kamperdijk, E C Hoefsmit

引用次数: 0

IL-1 mRNA expression detected by in situ hybridization and polymerase chain reaction in human thymus and thymoma. Correlation with late T-cell maturation in normal versus neoplastic thymus. 原位杂交和聚合酶链反应检测人胸腺和胸腺瘤组织中IL-1 mRNA的表达。正常胸腺与肿瘤胸腺晚期t细胞成熟的相关性。

Thymus Pub Date : 1993-08-01

C Aime, L Bristol, S Cohen-Kaminsky, S Berrih-Aknin, S Durum, L Takács

{"title":"IL-1 mRNA expression detected by in situ hybridization and polymerase chain reaction in human thymus and thymoma. Correlation with late T-cell maturation in normal versus neoplastic thymus.","authors":"C Aime, L Bristol, S Cohen-Kaminsky, S Berrih-Aknin, S Durum, L Takács","doi":"","DOIUrl":"","url":null,"abstract":"Interleukin-1 has long been suggested to participate in the development of T-cells in the thymus. To determine whether IL-1 was produced in the human thymus, we used the technique of in situ hybridization and PCR amplification of mRNA. High levels of IL-1 mRNA were detected in cells at the cortico-medullary border, which separates immature from mature T-cells, suggesting a role for IL-1 in a late stage in T-cell maturation. IL-1 transcripts were detectable by a single step PCR reaction in these samples. We examined a series thymomas (human thymic epithelial tumors) that induce partial T-cell maturation: In this group of thymomas T-cells develop to the cortical stage but not beyond. We failed to detect high levels of IL-1 mRNA expression in these tumors by in-situ hybridization, IL-1 mRNA was detected only in one of these samples by a single step PCR amplification. However, all of the samples were positive, when analyzed with a more sensitive, two step amplification of IL-1 mRNA by the use of nested primers. These results are consistent with the hypothesis that higher level of IL-1 expression is either the consequence of, or required for late T-cell maturation.","PeriodicalId":76738,"journal":{"name":"Thymus","volume":"22 1","pages":"45-54"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19291931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MHC-I non-restricted cytotoxic activity in Macaca sylvana experimentally inoculated with HIV2 and SIV/mac. 实验接种HIV2和SIV/mac的猕猴的MHC-I非限制性细胞毒活性。

Thymus Pub Date : 1993-08-01

B Charaf, K Sanhadji, S Sekkat, B Farouqui, J L Touraine, A Benslimane

{"title":"MHC-I non-restricted cytotoxic activity in Macaca sylvana experimentally inoculated with HIV2 and SIV/mac.","authors":"B Charaf, K Sanhadji, S Sekkat, B Farouqui, J L Touraine, A Benslimane","doi":"","DOIUrl":"","url":null,"abstract":"The anti-retrovirus cell-mediated immunity was repeatedly investigated in seven monkeys (Macaca sylvana). Four of these animals were injected with cell-free supernatants containing human immunodeficiency viruses: two monkeys received HIV1 Bru (2.5 x 10(6) cpm), two received HIV2 Rod (1.5 x 10(6) cpm). Two additional animals were injected with a cell-free supernatant containing simian immunodeficiency virus SIV/mac 251 (1.5 x 10(6) cpm) and the last animal served as control. The four macaques infected with HIV2 Rod and SIV/mac 251 seroconverted. Freshly isolated and non stimulated peripheral blood mononuclear cells from these infected macaques and from the uninfected control were repeatedly assessed for cytolytic activity. Target cells consisted of heterologous human cell lines expressing HIV1 Bru, HIV2 Rod or SIV/mac proteins. A significant cytotoxic activity, non-restricted at the major histocompatibility complex class I (MHC-I), was demonstrated in one HIV2 Rod-infected animal (F8) and in one SIV/mac 251-infected animal (M1). This last animal showed progressively diminishing cytolytic activity that was correlated with a pronounced decrease in CD4+ lymphocytes. An AIDS-like disease developed in M1, with presence of lymphadenopathy, weight loss, diarrhea and opportunistic infections. Cytotoxic activity was active against SIV and HIV2-infected target cells in an MHC-unrestricted manner; it was specific to virus-infected cells and there was cross-reactivity between HIV2 and SIV. Cytotoxic effectors appeared to be mainly CD8+ cells. This model may prove to be very useful in evaluating the capacity of candidate AIDS vaccines to elicit effective cell-mediated immune responses.","PeriodicalId":76738,"journal":{"name":"Thymus","volume":"22 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18904730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane translocation and relationship with MHC class I of a human thymic neurophysin-like protein. 人胸腺神经磷脂样蛋白的膜易位及其与MHC I类的关系。

Thymus Pub Date : 1993-08-01

V Geenen, E Vandersmissen, N Cormann-Goffin, H Martens, J J Legros, G Degiovanni, A Benhida, J Martial, P Franchimont

{"title":"Membrane translocation and relationship with MHC class I of a human thymic neurophysin-like protein.","authors":"V Geenen, E Vandersmissen, N Cormann-Goffin, H Martens, J J Legros, G Degiovanni, A Benhida, J Martial, P Franchimont","doi":"","DOIUrl":"","url":null,"abstract":"Thymic epithelial and nurse cells (TEC/TNC) synthesize an oxytocin (OT)-like peptide in association with a neurophysin (NP)-related protein in a way similar to in the hypothalamo-neurohypophysial (NHP) system. The central T-cell tolerance of the NHP neuroendocrine functions have been proposed to be mediated through these thymic NHP-related peptides due to their close homology with the NHP neurohormones OT and vasopressin (VP). In order to investigate their putative presentation by proteins of the major histocompatibility complex (MHC), human thymic membranes were purified and passed through an immunoaffinity column using mAb B9.12 directed to the monomorphic determinant of human MHC class I proteins. This methodology provided the following observations: (1) a NP-like protein is translocated in human thymic membranes and is retained by B9.12 on the column; (2) the MW of this NP-like material (50-55 kD) is quite different from the MW of hypothalamic NP proteins (10 kD), and (3) this thymic NP-like protein could be identified on Western blots with mAb B9.12. The precise extent of this relationship between the thymic NP-like protein and the Ig/MHC superfamily is actually investigated through the characterization of the genetic mechanisms responsible for the thymic expression of NHP-related peptides. Given the physiological importance of OT and of its binding to NP for transport along the axonal processes of the NHP tract, we postulate that, somewhat analogously, the thymic NP-/MHC class I-related protein could be involved in the presentation of the OT-like peptide to immature T-cells.","PeriodicalId":76738,"journal":{"name":"Thymus","volume":"22 1","pages":"55-66"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19291932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thymic stroma-derived T-cell inhibitory factor (TSTIF). 2: TSTIF acts on the antigen-presenting cell to inhibit antigen-stimulated T-cell proliferation. 胸腺基质源性t细胞抑制因子。2: TSTIF作用于抗原提呈细胞，抑制抗原刺激t细胞增殖。

Thymus Pub Date : 1993-06-01

X G Tai, Y Kita, K Toyooka, T Hamaoka, H Fujiwara

{"title":"Thymic stroma-derived T-cell inhibitory factor (TSTIF). 2: TSTIF acts on the antigen-presenting cell to inhibit antigen-stimulated T-cell proliferation.","authors":"X G Tai, Y Kita, K Toyooka, T Hamaoka, H Fujiwara","doi":"","DOIUrl":"","url":null,"abstract":"Culture supernatant (SN) was obtained from the monolayer of the MRL104.8a thymic stromal cell clone. This SN alone induced proliferation of helper T-cell (Th) clones because it contained IL-7. However, addition of the SN to cultures of Th stimulated with antigen plus antigen-presenting cells (APC) resulted in potent inhibition of their proliferation. This suppression was ascribed to a factor (designated thymic stroma-derived T-cell inhibitory factor, TSTIF) that is contained in the MRL104.8a SN and distinct from IL-7. TSTIF affected antigen-stimulated proliferation of both type 1 helper (Th1) and type 2 helper (Th2) T-cell clones. The TSTIF effect was also observed by the presence of the MRL104.8a SN only in the initial 24 hr pre-culture during the entire course (48-72 hr) of antigenic stimulation. Pre-exposure of Th cells to the SN in the absence of Ag/APC induced their proliferation upon stimulation with Ag/APC in the next 48 hr cultures. However, pre-cultures of Th cells with the SN in the presence of APC alone (without antigen) resulted in potent inhibition of the subsequent Ag/APC-stimulated proliferation. Interaction of TSTIF with APC but not with responding Th cells was further demonstrated in the following experiment: APC alone were exposed to the MRL104.8a SN and used for stimulation of Th that had not been exposed to the SN. Such an APC population exhibited a remarkably reduced capacity to induce antigen-stimulated Th proliferation when compared to that induced by freshly prepared APC or APC cultured in the absence of the MRL104.8a SN. These results indicate that TSTIF exerts its inhibitory effect on the antigen-stimulated T-cell proliferation by acting on APC.","PeriodicalId":76738,"journal":{"name":"Thymus","volume":"21 4","pages":"247-58"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19273506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cloned lymphoid Thy1+ tumor line derived from murine yolk sac cells maintained in long-term cell culture in the absence of a thymic microenvironment expresses an unusual cell surface phenotype. 来源于小鼠卵黄囊细胞的克隆淋巴细胞Thy1+肿瘤系在缺乏胸腺微环境的长期细胞培养中表达了一种不寻常的细胞表面表型。

Thymus Pub Date : 1993-06-01

C P Liu, A Globerson, R Auerbach

引用次数: 0

Two novel monoclonal antibodies reactive with different components of the rat thymic epithelium. 两种新型单克隆抗体与大鼠胸腺上皮不同成分反应。

Thymus Pub Date : 1993-06-01

M D Pavlović, M Colić, D Vucević, A Dujić

引用次数: 0