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Identification and characterization of an avian reovirus isolated from black-tailed gull (Larus crassirostris). 黑尾鸥(Larus crassirostris)禽呼肠孤病毒的鉴定和特性分析。
The Kitasato archives of experimental medicine Pub Date : 1989-12-01
K Takehara, C Kawai, A Seki, N Hashimoto, M Yoshimura
{"title":"Identification and characterization of an avian reovirus isolated from black-tailed gull (Larus crassirostris).","authors":"K Takehara,&nbsp;C Kawai,&nbsp;A Seki,&nbsp;N Hashimoto,&nbsp;M Yoshimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An isolated virus from the feces of nestlings of Black-tailed gulls living on the Kabu-Island, Hachinohe-city, Aomori-prefecture, was identified as an avian reovirus from its morphological, physicochemical and biological features. Serologically, the isolate has a 62% of relatedness to TS-17 strain, a prototype of avian reovirus in Japan. The isolate showed no significant virulences to one-day-old SPF chickens, and showed low mortalities to chicken embryos, although it formed remarkable lesion onto chorioallantoic membrane.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"187-98"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas fluorescens isolated from the diseased rainbow trout, Oncorhynchus mykiss. 患病虹鳟荧光假单胞菌的分离。
The Kitasato archives of experimental medicine Pub Date : 1989-12-01
M Sakai, S Atsuta, M Kobayashi
{"title":"Pseudomonas fluorescens isolated from the diseased rainbow trout, Oncorhynchus mykiss.","authors":"M Sakai,&nbsp;S Atsuta,&nbsp;M Kobayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pseudomonas fluorescens was isolated from the diseased rainbow trout, Oncorhynchus mykiss, in Iwate Prefecture. Typical symptoms of the diseased fish were hemorrhage at the bases of fin and anal region, and internally, petechia of each organ, especially the intestine. This bacterium was pathogenic to rainbow trout and Tilapia, Sarotherodon niloticus.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"157-62"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolutionary aspects of pituitary hormones. 垂体激素的进化方面。
The Kitasato archives of experimental medicine Pub Date : 1989-12-01
H Kawauchi
{"title":"Evolutionary aspects of pituitary hormones.","authors":"H Kawauchi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"139-55"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13842587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of Hymenolepis nana infection on antibody responses to sheep red blood cells in mice. 微小膜膜绦虫感染对小鼠羊红细胞抗体反应的影响。
The Kitasato archives of experimental medicine Pub Date : 1989-12-01
M Shinoda, K Asano
{"title":"The influence of Hymenolepis nana infection on antibody responses to sheep red blood cells in mice.","authors":"M Shinoda,&nbsp;K Asano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of Hymenolepis nana infection on the primary antibody responses to both sheep red blood cells (SRBC) and dinitrophenylated Ficoll-coated SRBC (DNP-coated SRBC) was examined in BALB/c mice. The in vitro cultures of spleen cells (Sp C) obtained from mice inoculated with either 100 or 1,000 eggs developed fewer plaque forming cells (PFC) than those of Sp C from non-infected mice, when the cells were exposed to SRBC. Thereafter, the number of PFC was gradually increased and almost reached the initial levels by day 21. On the other hand, the ability of Sp C obtained from mice infected with 10,000 eggs to produce PFC gradually dropped as the course of infection proceeded. However, H. nana infection did not reduce the capacity of Sp C to produce PFC to DNP-coated SRBC. Addition of T-cell enriched population from infected mice, which was obtained by passing through a nylon wool column, to anti-Thy-1.2 antibody treated non-infected Sp C significantly inhibited the production of PFC to SRBC. These results strongly suggest that H. nana infection impairs T-cell function in mice.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"163-9"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13678607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Survival of microfilariae released in vitro by the filarial worm, Dipetalonema viteae. 微丝虫体外释放微丝虫的存活。
The Kitasato archives of experimental medicine Pub Date : 1989-12-01
J Maki, P P Weinstein
{"title":"Survival of microfilariae released in vitro by the filarial worm, Dipetalonema viteae.","authors":"J Maki,&nbsp;P P Weinstein","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"203-5"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13783237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Shibasaburo Kitasato and Sahachiro Hata]. [shiwet buro Kitasato和Sahachiro Hata]。
The Kitasato archives of experimental medicine Pub Date : 1989-09-01
W Köhler
{"title":"[Shibasaburo Kitasato and Sahachiro Hata].","authors":"W Köhler","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 2-3","pages":"85-106"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13837629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reactions of the lectin anti-AHP from the edible snail Helix pomatia with N-acetyl-D-galactosamine of streptococci. 食用螺蛳凝集素抗ahp与链球菌n -乙酰- d -半乳糖胺的反应。
The Kitasato archives of experimental medicine Pub Date : 1989-09-01
W Köhler, T Nagai
{"title":"Reactions of the lectin anti-AHP from the edible snail Helix pomatia with N-acetyl-D-galactosamine of streptococci.","authors":"W Köhler,&nbsp;T Nagai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The lectin anti-AHP obtained from the albumen gland of the edible snail, Helix pomatia, was used for the routine identification of group C streptococci. Without any exception all 1,045 strains of group C streptococci (S.equisimilis, and S.zooepidemicus, and S.dysgalactiae) were agglutinated by the Helix lectin, but none of the 12,264 strains of group A, 1,346 strains of group G, 330 strains of group B as well as that of other streptococcal groups. The presence of type-specific T antigens of group A streptococci in group C streptococci does not influence the agglutinability of S.equisimilis (group C). A few but not all strains of S.milleri either carrying or not group G specific carbohydrate and S.sanguis with group H antigen were agglutinated by the Helix lectin Anti-AHP. This suggests that terminal N-acetyl-D-galactosamine of these bacteria reacted with the Helix lectin (Anti-AHP). Therefore the Helix lectin is very useful tool for identification of group C streptococcus as well as for immunological studies.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 2-3","pages":"107-13"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13763949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Measurement of small quantities of alpha 2-macroglobulin in cell cultures by enzyme labelled immunoassay. 用酶标记免疫分析法测定细胞培养中少量α 2巨球蛋白。
The Kitasato archives of experimental medicine Pub Date : 1989-09-01
M Takasaki, K Hachimura, T Funato, T Nishikawa, H Ohtani
{"title":"Measurement of small quantities of alpha 2-macroglobulin in cell cultures by enzyme labelled immunoassay.","authors":"M Takasaki,&nbsp;K Hachimura,&nbsp;T Funato,&nbsp;T Nishikawa,&nbsp;H Ohtani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We established an enzyme labelled immunoassay (EIA) for the determination of alpha 2-macroglobulin (alpha 2M). The sensitivity was high enough to measure trace amount of alpha 2M secreted into the medium of cell culture. The assay range in medium was from 2 to 140 ng/ml and the within-assay coefficients of variation (CV) were 10.1% and 6.7% at 31.2 ng/ml and 62.5 ng/ml, respectively. Between-day CV ranged from 9.3% to 15.9%. Using this method, alpha 2M was detected in the medium of a cultured human melanoma cell line. In addition, the immunoreactive alpha 2M in the medium was identified with serum alpha 2M by the method of SDS-PAGE and Western blotting. Our results indicate that the EIA method could be a useful tool for the determination of alpha 2M in cell cultured medium.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 2-3","pages":"115-22"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low responsiveness of lymphocytes from normal individuals against the stimulation with pokeweed mitogen. 正常人淋巴细胞对商陆有丝分裂原刺激的低反应性。
The Kitasato archives of experimental medicine Pub Date : 1989-09-01
T Funato, Y Bandou, H Ohtani
{"title":"Low responsiveness of lymphocytes from normal individuals against the stimulation with pokeweed mitogen.","authors":"T Funato,&nbsp;Y Bandou,&nbsp;H Ohtani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The responsiveness of peripheral blood lymphocytes stimulated with pokeweed mitogen (PWM) and purified protein derivative of tuberculin (PPD) as B cell activator was studied in adult healthy subjects by immunoglobulin synthesis system in culture. We have shown that lymphocytes from some healthy subjects have low responsiveness in the polyclonal antibodies secretion following PWM stimulation but not PPD stimulation by Protein A-reverse hemolytic assay. In this system, when irradiated T cells (CD4+) as helper T cell were added to B cells from autologous or allogeneic subjects, helper T cell function of immunoglobulin synthesis on the low response groups to PWM significantly decreased. Whereas normal responses to PPD stimulation were observed in the presence of helper T cells (CD4+). The low responsiveness of lymphocytes stimulated with PWM but not PPD may be related to reduced functions of helper T cell populations. Thus the healthy subjects were classified into high, normal and low response groups with PWM on the basis of antibody production.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 2-3","pages":"129-33"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13763951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological observations of Babesia ovata in bovine lymphocyte. 牛淋巴细胞卵形巴贝斯虫的形态学观察。
The Kitasato archives of experimental medicine Pub Date : 1989-09-01
S Higuchi, K Tazawa, K Matuda, S Kawamura, Y Yasuda
{"title":"Morphological observations of Babesia ovata in bovine lymphocyte.","authors":"S Higuchi,&nbsp;K Tazawa,&nbsp;K Matuda,&nbsp;S Kawamura,&nbsp;Y Yasuda","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 2-3","pages":"135-8"},"PeriodicalIF":0.0,"publicationDate":"1989-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13763952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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